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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 1 nM TO-PRO™-3 Iodide (642/661 nm, Thermofisher) and 8 ng/ml calcofluor-white (CW ...
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bioRxiv - Developmental Biology 2019Quote: ... Nuclei were labeled using TOPRO-3 (1:1000, T3605, Life Technologies). Brains were mounted for confocal microscopy in Vectashield (Vector Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were collected into FAD media (DMEM/F12 3:1 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2019Quote: ... 1 cm pieces and washed 3 times with 1x HBSS (Gibco) containing 15 mM HEPES (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: ... for RPE1] in 1:3 ratio in Opti-MEM media (Gibco), incubated for 15 min at RT ...
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bioRxiv - Neuroscience 2022Quote: Neurons were transfected at DIV 1-3 using Lipofectamine 2000 (Invitrogen). For one Ø18-mm coverslip seeded with 100,000 neurons ...
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bioRxiv - Immunology 2021Quote: ... and goat anti-mouse Cyanine 3 (Life technologies, A10521, 1:1000). Images for BrdU staining were taken using a Zeiss Confocal (LSM710 META) ...
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bioRxiv - Microbiology 2020Quote: ... 3 μl of DNA ladder (1 Kb Plus DNA Ladder; Invitrogen), 6 μl of positive control and 10μl of template DNA were ran on 2.5% (w/v ...
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bioRxiv - Genomics 2021Quote: ... Linker oligo sequences were: 5’ – TTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNCAGGCTACTCCGCTTAAGGGAC-3’ (linker 1, Invitrogen, UK) and 5’-GTCCCTTAAGCGGAGTAGCCTG/3AmMO/-3’ (linker 2 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μM FluoZin-3 tetrapotassium salt (Thermo Fisher Scientific, Waltham, MA) was then added to each reaction ...
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bioRxiv - Cell Biology 2022Quote: ... Incubation with TO-PRO-3 (1:100, ThermoFisher Scientific Ref. T3605) was performed along with secondary antibody incubation to stain nucleic acid ...
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bioRxiv - Immunology 2022Quote: ... 3 µl of Dynabeads MyOne Carboxylic Acid (1 μm; ThermoFisher Scientific) were added at a concentration of 0.5 mg/ml to each of the samples ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was labeled with ToPro-3 (1:5000; Invitrogen, Cat #T3605) in 0.3% PBST for 30 min at RT ...
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bioRxiv - Immunology 2024Quote: ... were coated with 3 µg ml-1 of streptavidin (Thermo Fisher) diluted in carbonate-bicarbonate buffer (E107 ...
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bioRxiv - Genomics 2022Quote: ... at a 1:3 ratio in Opti-MEM medium (Gibco, 11524456), incubated for 15 minutes at room temperature and added dropwise to the cells ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-14-3-3γ 1:2000 (Thermo fisher PA5-29690) and mouse anti-GAPDH (CSB-MA000195 ...
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bioRxiv - Microbiology 2023Quote: ... HSV-1 Probe FAM-5’-CGGCCCAACATATCGTTGACATGGC-3’-MGBNFQ (Thermo Fisher Scientific). The efficiency of each round of PCR was determined using 10-fold dilutions of Topo TA plasmids (Invitrogen AB ...
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bioRxiv - Physiology 2023Quote: ... counterstained with 1:30,000 TO-PRO-3 Iodide (Life Technologies, T3605), and then mounted in SlowFade Diamond mounting medium (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... Cy-3 conjugated anti-rabbit secondary antibody (Invitrogen, 1:300 dilution) was added and incubated for 0.5 hr ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were immersed in 3 µM FM 1-43FX (ThermoFisher, F25255) in E3 for 30 s ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 2 h at RT with an Alexa Fluor 555 nm-conjugated goat anti-mouse secondary antibody (1:500; Molecular Probes, ThermoFisher Scientific cat#A21127; RRID: AB_141596) in blocking solution ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 1 h using with secondary antibodies conjugated to Alex Fluor-546 or 647 of appropriate species (1:500, ThermoFisher Scientific). After several washes in PBS sections were mounted on glass slides using Fluoromount-G ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mg of striatal lysates was pre-cleared for 1 h with 80 ul Protein G Sepharose coated beads (ThermoFisher Scientific), then centrifuged at 4°C for 5 min at 1000 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... antibody at 5 μg mL−1 in BSA 1% m v−1 in PBS for 1 h at room temperature, then with anti-rabbit Alexa 568 (LifeTech, A11036) and Hoechst (Invitrogen, H3570) 1 μg mL−1 in BSA 1% m v-1 in PBS for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... and incubated for 1 h at RT with a mixture of Alexa Fluor 594 conjugated anti-rabbit IgG (1:500; A11012; ThermoFisher, USA) and Alexa Fluor 488-conjugated anti-mouse IgG (1:500 ...
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bioRxiv - Genetics 2020Quote: ... then incubated with DAPI (1:5000) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibodies (1:200; Thermo Fisher). After incubation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 × 10 min with PBS and incubated for 1 h at room temperature with the secondary antibody (goat anti-rabbit Alexa Fluor 488, 1:1000; Invitrogen, Germany) diluted in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... bound antibody was detected by incubation for 1 h with Alexa Fluor 546-IgGs or Alexa Fluor 488-IgGs (1:200 for both, Invitrogen, USA).
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bioRxiv - Cell Biology 2020Quote: ... cells were subsequently incubated with secondary antibodies for 1 h and nuclei visualized with 1 μg/ml DAPI (Invitrogen, D-1306). All incubations were performed at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... sections were washed and incubated with for 1 h at room temperature with secondary antibodies goat anti-rabbit Alexa Flour 568 (1:1000, A11011; Life Technologies) for Nestin ...
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bioRxiv - Microbiology 2020Quote: ... followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific). Bands were detected by chemiluminescence using ECL Western blotting detection reagent (Amersham Bioscience ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM 2-mercaptoethanol and incubated for 1 h at 37 °C with 1 mM EDTA (ethylenediaminetetraacetic acid) and RNase A/T1 (ThermoFisher Scientific) (1:100) ...
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bioRxiv - Microbiology 2019Quote: ... A final washing step after 1 h incubation at TR was followed by addition of 100 µl 1-Step Slow TMB-ELISA substrate (Thermo Scientific) to each well and absorbance was measured after 10 min incubation at TR using the Bio-Rad Model 680 Microplate Reader (655 nm ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were washed for 15 min in TBST and then incubated for 1 h with HRP-conjugated secondary antibodies (1:5,000; Pierce Antibodies; Thermo Fisher Scientific) in TBST at RT ...
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bioRxiv - Cell Biology 2019Quote: ... in 0.5% BSA PBS at room temperature for 1 h prior to incubation with AlexaFluor 594 or 488 anti-rabbit secondary antibodies (1:5000, Molecular Probes) in 0.5% BSA PBS ...
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bioRxiv - Systems Biology 2019Quote: ... Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 647-conjugated goat anti-mouse IgG diluted in washing solution for 1 h (1:100, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated in absence of light for 1 h with fluorescent goat anti-mouse (1:500, Alexa Fluor 488, Life Technologies) diluted in TBS with 3% BSA ...
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bioRxiv - Physiology 2021Quote: ... The muscle sections were then washed three times in PBS before being incubated for 1 h in the dark at room temperature with Alexa Fluor 488 IgG secondary antibody (A-11008, 1:500; ThermoFisher Scientific) diluted in blocking solution ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-FLAG antibody was detected using an anti-mouse Alexa 647 secondary antibody (Alexa 488 goat anti-mouse, 1:500 dilution, RT, 1 h, Invitrogen, #A11029). The images were captured with an InCell2000 imager.
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bioRxiv - Plant Biology 2024Quote: ... the membrane was blocked with 5% bovine serum albumin in PBS with 0.05% Tween-20 for 1 h and then incubated with HRP-conjugated streptavidin (1:20000, Thermo Fisher Scientific) with shaking for 1 h at room temperature or 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with TBS-T and incubated for 1 h at room temperature with an HRP-conjugated anti-mouse (dilution 1:50,000, Invitrogen, G-21040) or anti-rabbit (dilution 1:50,000 ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_2893433), tdTomato (SICGEN, 1:500, RRID: AB_2722750) donkey anti-goat IgG (H+L) Alexa Fluor 488 (Invitrogen, 1:1000, A11057), Cy3 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: Age synchronized worms were washed thrice with 500 μl M9 buffer in 1.5 ml microfuge tube and incubated in dark for 1 h in 1 ml M9 containing 5 µl DiD dye (Vybrant™ DiD Cell-Labeling Solution, ThermoFisher) at ∼20-30 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed with TBST and incubated with the required fluorescent-dye conjugated secondary antibody (1:1000) for 1 h at RT (Alexafluor, Invitrogen, Thermofisher). Slides were mounted with DAPI Pro-LongDiamond antifade mountant overnight and analyzed the next day ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies were used at 1:1000 at RT for 1 h with rotation (Anti-rabbit-HRP, Invitrogen, anti-Armenian Hamster-HRP, Thermo Fisher). Blots were imaged using an Amersham Imager 600 ...
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bioRxiv - Bioengineering 2022Quote: ... sections were incubated for 1 h at RT with goat anti-mouse Alexa 555-conjugated antibody (1:300; A21434, Life Technologies). After counterstaining with hematoxylin slides were mounted under coverslips in Prolong Gold anti-fade reagent (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 h (Western blot) or 30 min (MS) at 170 V in 1× NuPAGE MOPS SDS running buffer (Thermo Scientific). The Colloidal blue staining kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000, Thermo Fisher Scientific). Images were acquired using the Keyence BZ-X800.