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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Genetics 2021Quote: ... Slides were washed 3 times in Phosphate Buffered Saline (PBS) prior to mounting coverslips using Prolong Gold Antifade Mountant with DAPI (Invitrogen). Slides were imaged on a Nikon Confocal A1R microscope for image processing using NIS-Elements or ImageJ software.
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bioRxiv - Bioengineering 2021Quote: ... decellularized apple scaffolds (prior to MC3T3-E1 cell seeding) (n=3) were thoroughly washed with phosphate buffered saline (PBS; ThermoFisher) and incubated in 1 mL of 10% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... After primary and secondary antibody incubation the membrane was washed 3×15 minutes in PBS-T (Phosphate buffered saline (Gibco), 0.1% Tween-20) ...
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bioRxiv - Microbiology 2020Quote: ... with one or two steel beads of 3 mm (Dejay Distribution, UK) [66] in 200 µl Dulbecco’s phosphate buffered saline (PBS, 14190094, Life Technologies), supplemented with penicillin-streptomycin and amphotericin B ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or ribosomal protein large P0 (RPLP0) endogenous transcripts (Thermo Fisher Scientific; 4310884E; 4310879E) and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... permeabilized with cold methanol for 3 min and 0.3% Triton X-100 in phosphate buffered saline (PBS, pH 7.4, Thermo Fisher Scientific) and blocked with 10% normal goat serum (NGS ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissolved tissue was subsequently washed 3 times with 1% FBS solution in 1x DPBS (Dulbecco’s phosphate-buffered saline, ThermoFisher Scientific) and passed through 75 μm and 35 μm cell strainers to remove debris.
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bioRxiv - Bioengineering 2023Quote: ... A separate qPCR reaction for each sample was performed to detect the NHP cDNA of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) using the Taqman Gene Expression Assays 20x mix (assay ID Mf04392546_g1, ThermoFisher Scientific). This probe spans exons ...
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bioRxiv - Molecular Biology 2024Quote: ... we sectioned brains as 30μm free floating sections and then blocked for 1 hour in phosphate-buffered saline with 0.1% Triton X-100 and 3% normal donkey serum (Fisher Scientific). We incubated the sections overnight at room temperature in chicken anti-GFP (GFP-1020 ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were also probed with a mouse monoclonal antibody targeting glyceraldehyde-3-phosphate dehydrogenase (mAb-α-GAPDH; 1:8000; ThermoFisher).
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bioRxiv - Systems Biology 2024Quote: ... fresh medium was added to dilute the tryplE and cells were centrifuged at 400 x g for 3 min then washed twice with Phosphate Buffered Saline (PBS) (Gibco) with centrifugation in-between ...
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bioRxiv - Genomics 2021Quote: ... cells were seeded in 6-well plates or 6 cm dishes and reverse transfected with 5 nM Silencer Select siRNAs (Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...
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bioRxiv - Genomics 2021Quote: ... 600,000 cells were plated in 6 well plates and transfected with 2 μg plasmid DNA using 6 μl Lipofectamine LTX reagent (Thermo Fisher). Medium was exchanged after 16 h and harvested 24 h later ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 was depleted using siRNA oligo #14 5’-CGGAAGACCUGCCGUUACA-3’ (ThermoFisher). siRNA oligos for PP2A-B55 and PP2A-B56 have been described (Hayward et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 3-5 × 105 cells were settled on Polysine Slides (Thermo Fisher), fixed with 4% ...
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bioRxiv - Neuroscience 2020Quote: ... FIVNC-555p (5’-FAM-CATGGCCACATTAATAATGG CGCA -TAMRA-3’ (Applied Biosystems, CA). These reactions were performed with a Bio-Rad iCyclerTM iQ and analyzed using the manufacturer’s software ...
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bioRxiv - Cell Biology 2022Quote: ... DLP1-sense strand: 5’-UCCGUGAUGAGUAUGCUUUdTdT-3’ 31 (Ambion, Austin, TX, USA).
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bioRxiv - Cell Biology 2020Quote: ... siRNA against MyoVa was obtained from Invitrogen (s9207, 5’ GUAUAGUCCUAGUAGCUA 3’) as this was shown to work well by Wu et al (2018) ...
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bioRxiv - Immunology 2020Quote: ... Reactions were run on a Quantstudio 3 or 5 instrument (ThermoFisher). Cycling conditions for Quantifast reagents were ...
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bioRxiv - Genomics 2021Quote: ... Linker oligo sequences were: 5’ – TTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNCAGGCTACTCCGCTTAAGGGAC-3’ (linker 1, Invitrogen, UK) and 5’-GTCCCTTAAGCGGAGTAGCCTG/3AmMO/-3’ (linker 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were passaged every 3-5 days using TryplE (Gibco 12604054). Naïve and primed hPSCs were expanded and induced into different lineages in a 5% CO2 incubator at 5% O2 at 37C.
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bioRxiv - Neuroscience 2023Quote: ... or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) using the 4D-Nucleofector (P3 Primary Cell Solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5’- TTGGATCAGCTCAGACATATT-3’ or a nonspecific “scrambled” control (Invitrogen, United States). 72 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 3 days after transfection by 5 µl of lipofectamine 2000 (Invitrogen) with 2 µg of the plasmid and regularly re-sorted to maintain expression of myr/palm-mCherry.
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bioRxiv - Microbiology 2023Quote: ... HSV-1 Probe FAM-5’-CGGCCCAACATATCGTTGACATGGC-3’-MGBNFQ (Thermo Fisher Scientific). The efficiency of each round of PCR was determined using 10-fold dilutions of Topo TA plasmids (Invitrogen AB ...
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bioRxiv - Cell Biology 2024Quote: ... The siRNA oligonucleotide targeting RIF1 sequence (Invitrogen; Sense: 5’-GAAUGAGCCCCUAGGGAAATT-3’) 138 was used ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Cell Biology 2024Quote: Isolated CDX2mCherry cells (n=5) mCherry-negative cells (n=4) and hES cells (n=2 pooled) were centrifuged and 200uL Tri reagent (Thermo Fisher Scientific, USA) was added and incubated at room temperature for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... Intestinal samples were fixed in paraformaldehyde (PFA 32%, Fischer Scientific) 4% in phosphate buffer saline (PBS, Gibco, Waltham, MA) for 1 hour at 20°C ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed with 4% formalin solution (neutrally buffered) and kept in phosphate buffered saline (PBS, Gibco no. 10010023) during the experiment ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 4% formalin solution (neutrally buffered) and kept in phosphate buffered saline (PBS, Gibco no. 10010023) during the experiment.
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 4% formalin solution (neutrally buffered) and kept in phosphate buffered saline (PBS, Gibco no. 10010023) during the experiment.
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bioRxiv - Physiology 2024Quote: ... Excised parotid glands were fixed in 4 % of paraformaldehyde (PFA) in phosphate-buffered saline (PBS) (J19943-K2, Thermo scientific) for 48 hours at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and then were fixed in 4% paraformaldehyde in 0.1M phosphate-buffered saline (PBS; Fisher Scientific, Fair Lawn, NJ, USA) at pH 7.4 with 10-12% sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... in phosphate buffered saline (PBS) for 15 min at room temperature or in 100% methanol (A412-4, Fisher Scientific) for 5 min at -20°C ...
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bioRxiv - Cell Biology 2023Quote: ... in phosphate buffered saline (PBS) for 15 min at room temperature or in 100% methanol (A412-4, Fisher Scientific) for 5 min at -20°C ...
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bioRxiv - Biochemistry 2023Quote: NMA1982 phosphatase activity was measured using a standard fluorescence intensity phosphatase assay utilizing 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP; Invitrogen) as the substrate [26] ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM MnCl2 with a final assay concentration of 75 µM 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) (Invitrogen D6567). Generation of 6,8-difluro-4-methylumbelliferone (DiFMU ...
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bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5 × Vilo reaction mix (Life Technologies, 11754), and 2 μl of 2 × Superscript III enzyme blend (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Neutrophils were loaded with Fluo-4/AM (5 μM, Invitrogen) in the presence of Pluronic F-127 (0.02% ...
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bioRxiv - Immunology 2023Quote: ... High 5 cells (BTI-Tn-5B1-4; Thermo Fisher Scientific) were infected with virus particles ...
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bioRxiv - Genetics 2024Quote: ... were passaged every 4-5 days using Accutase (Thermo Fisher) in the presence of 1uM Thiazovivin (THZ ...
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bioRxiv - Cell Biology 2024Quote: ... and passaged every 4-5 days with EDTA (ThermoFisher Scientific). Media change was performed every 24 hours (h ...
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bioRxiv - Developmental Biology 2023Quote: ... the corneas were cut into 2×2 mm pieces and sterilized by soaking in 1X Gibco phosphate buffered saline (PBS) (Fisher Scientific, 10-010-023) containing 1% HyClone penicillin streptomycin 100X solution (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The RNA of 500 µL iRBCs at 3-5% parasitaemia was isolated using 3 mL TRIzol reagent (Invitrogen) followed by phenol-chloroform phase separation ...