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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: A 3 or 5 wt% solution of GelMAL was prepared by dissolving GelMAL in PBS (Gibco Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with siRNA (5’-GGACTGAGGUUGUCAAGAA-3’) for PRC1 using Oligofectamine (Life Technologies, Carlsbad, CA) as previously described (Udy et al. ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Microbiology 2022Quote: ... Amplifications were achieved using a 3-step program on a QuantStudio 5 system (Thermo Fisher Scientific). Transcript abundance was calculated using the ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was carried out on a QuantStudio 3 or QuantStudio 5 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged approximately every 3-5 days (70-85% confluency) using Accutase (Gibco, #11105-01) to detach cells ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Physiology 2023Quote: ... R: 5’ TGCCTTCGTGCCGTACTG 3’) and confirmed by DNA sequencing following sub-cloning into pCRTM-Blunt (Invitrogen). Further genotypes were determined by real-time PCR with specific probes designed for each gene (Transnetyx ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Physiology 2023Quote: ... were incubated for 20 minutes with 5 µM of Di-3-ANEPPDHQ (Thermo Fisher Scientific, USA) membrane dye ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid containing a sgRNA targeting APC (5‘-GGCACTCAAAACGCTTTTGA-3‘) using lipofectamin-2000 (Thermo Fisher Scientific, 11668027). WT organoids were grown in WENR medium and APC truncated organoids were grown in ENR medium and are later refererred to as mCol WT and mCol APC ...
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bioRxiv - Neuroscience 2021Quote: ... The assay was performed using technical duplicates per sample in 384-well optical plates with ABI Prism 7900 Sequence Detection system (Applied Biosystems, Life Technologies) following the supplier’s parameters ...
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bioRxiv - Cancer Biology 2021Quote: ... Single clones were obtained by serial dilution in 96 well plates and KO clones were identified by Indel Detection by Amplicon Analysis (IDAA) using ABI PRISM™ 3010 Genetic Analyzer (Thermo Fisher Scientific) and Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR was conducted with the ABI PRISM 7900HT Sequence Detection System for a 384-well plate loaded with SYBR green (ThermoFisher Scientific, catalog #4309155), cDNA ...
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bioRxiv - Molecular Biology 2019Quote: Constructs based on the pcDNA5 vector (Thermo Fisher Scientific) were used to express fulllength HSPA1A and HSPA8 with N-terminal V5 and BirA epitope tags and a C-terminal in-frame K48R ubiquitin (pTP3923 and pTP3921 ...
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bioRxiv - Cell Biology 2019Quote: ... miRNA expression was assessed by TaqMan-based qPCR (ThermoFisher, Part Number 4427975 ...
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bioRxiv - Cell Biology 2022Quote: ... A Tali Image-based Cytometer (ThermoFisher Scientific; Waltham, MA) was used to calculate the portion of cells in suspension that incorporated propidium iodide ...
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bioRxiv - Cancer Biology 2022Quote: ... eluted in PBS-based antibody stabilizer (Thermo Fisher, #NC0436689) containing 5 mM EDTA and 0.1% NaN3 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... permeabilized with a saponin-based reagent (Life Technologies, C10419) before intracellular staining with an anti-KRT5 antibody (AF-488 ...
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bioRxiv - Neuroscience 2020Quote: A fluorescence-based KDalertTM GAPDH assay (Invitrogen, Life Technologies) was used to measure enzymatic activity of GAPDH protein of extracellular vesicles ...
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bioRxiv - Neuroscience 2020Quote: A fluorescence-based KDalertTM GAPDH assay (Invitrogen, Life Technologies) was used to measure enzymatic activity of GAPDH protein of extracellular vesicles ...
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bioRxiv - Genetics 2020Quote: ... we used the lipid-based Lipofectamine 3000 (Thermo Fisher) transfection method due to its low cost and feasibility ...
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bioRxiv - Evolutionary Biology 2021Quote: ... based medium supplemented with N2 (Invitrogen, cat. no. 17502048), B27 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... A SYBR Green-based master mix (ThermoFisher Scientific, A25778) was applied for RT-PCR analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... and transfected by the Calcium Phosphate-based method (Invitrogen). To study the effects of different Cas13 variants ...
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bioRxiv - Cell Biology 2022Quote: ... the resazurin-based solution Alamar Blue (Thermo Fisher Scientific) is added and the cells are incubated for 3h at 37°C to allow for the metabolic conversion of resazurin to resorufin ...
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bioRxiv - Neuroscience 2022Quote: ... Differentiation medium was G-MEM-based (Gibco, 21710-025) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Plant Biology 2022Quote: ... an LR gateway-based recombination reaction (Invitrogen, CA, USA) with either pYFC43 or pYFN43 was carried out ...
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bioRxiv - Microbiology 2023Quote: DNA-based staining using SYBR Green I (SG; Invitrogen) in combination with propidium iodide (PI ...
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bioRxiv - Neuroscience 2023Quote: ... based on E8 Flex/VTN (ThermoFisher, #A2858501 and #A14700), CRISPR/Cas9-based genome editing ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in 1x DMEM based media (ThermoFisher Scientific) as described above ...
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified with SYBR-based qPCR (ThermoFisher #AB0765). Circularized libraries were denatured and diluted for a target loading concentration of 6 pM with 2% PhiX ...
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bioRxiv - Immunology 2023Quote: ... Multiplex bead-based measurements of cytokines (Luminex, Thermo Fisher) were performed by the UCLA Immune Assessment Core.
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bioRxiv - Genetics 2024Quote: ... based EB medium included 20% Knock-out Serum (Gibco), 3% ES-FBS (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 2 hours prior to splenocyte harvest ...
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bioRxiv - Developmental Biology 2021Quote: ... with 50-100 nl of 1 mM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) at stage 41 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were counterstained with 2 μM CellTracker Green (5-chloromethylfluorescein diacetate dye) (ThermoFisher) followed by NucBlue (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... NPE-caged ATP (adenosine 5’-triphosphate, P3- (1-(2-nitrophenyl ethyl) ester) (Invitrogen) dissolved in attachment buffer was photolyzed in the AFM chamber using an UV light source at 340 nm ...
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Microbiology 2020Quote: ... 5 mM tris(2-carboxyethyl)phosphine (TCEP, Bond-Breaker, neutral pH, Thermo Scientific) was added and samples were incubated at room temperature for 20 min ...
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bioRxiv - Immunology 2019Quote: ... cells were loaded with 5 mM Fura-2-AM/neurobasal media (Thermo Fisher) at 37°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 3.5 hours prior to splenocyte harvest ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... monensin (5 μM) and retro-2 (100 μM) were obtained from Molecular Probes [B7450] ...