Labshake search
Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... and labeled using the Pierce RNA 3′ End Desthiobiotinylation Kit (ThermoFisher Scientific). HOXC13-AS pulldown assay was performed using the Pierce Magnetic RNA-Protein Pull-Down Kit (ThermoFisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... The Silencer™ siRNA Labeling Kit with Cy™3 dye (Invitrogen) was used to label dsRNA following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... which were biotin-labeled using a GeneChip 3’ IVT express kit (Affymetrix). Microarray analysis was performed at TaKaRa-Bio using high-density oligonucleotide array (Human Genome U133 Plus 2.0 ...
-
bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
-
bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
-
bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
-
bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
-
bioRxiv - Biophysics 2023Quote: 50 µl of AP205 VLP solution (5 µg/ml) was pipetted into an appropriate number of wells of ELISA plates (4 Nunc MaxiSorp flat bottom 96-well plates) and the VLP allowed to adsorb overnight at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5′ capping (mMESSAGE mMACHINE™ T7 Transcription Kit, Thermo Fisher), and 3′ poly(A ...
-
bioRxiv - Microbiology 2023Quote: ... the 5’ RACE (Rapid Amplification of cDNA Ends, Invitrogen kit) technique was used ...
-
bioRxiv - Cancer Biology 2020Quote: ... The purified RNA obtained from IVT was labelled with biotin at the 3’end using the PierceTM RNA 3′End Desthiobiotinylation Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2020Quote: AAG AGC TCT TCA GAA ATG GAT GCC ATG TGT GTC TCC TCT TCC TGA-3’) derived from the intron 10 of PIK3C2B were biotin-labeled using Biotin 3’-End DNA Labeling Kit (Thermo Scientific). Biotin-labeled ssODNs were then annealed with reverse complimentary ssODNs to generate biotin-labeled probes ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... and IL-2 using ELISA kits provided by ThermoFisher. The characterizations followed the manufacturer’s protocols ...
-
bioRxiv - Microbiology 2022Quote: ... the TOPO XL-2 Complete PCR Cloning Kit (Invitrogen) was used according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... the IL-2 Mouse ProQuantum Immunoassay Kit (Invitrogen, A42892) was used.
-
bioRxiv - Cell Biology 2019Quote: ... using 3–5 µg of primary antibody against each protein and protein-G magnetic Dynabeads (10003D, Invitrogen) and incubated overnight at 4°C on rotation ...
-
bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... HCT116 cells complemented with chromosomes 3 and 5 were cultured with 400 μg/mL geneticin (G418, Gibco) and 6 ug/mL blasticidine (Invivogen) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Staining of cells was performed using 5 μl of DiBAC4(3) (Invitrogen, 0.025 mg/ml in DMSO) followed by incubation for 15 minutes at room temperature in dark ...
-
bioRxiv - Microbiology 2019Quote: ... samples were washed 3 times in PBS (5 min each time) and counterstained with SYTO9 (S34934, ThermoFisher). ProLong™ gold antifade mountant (Thermofisher ...
-
bioRxiv - Physiology 2019Quote: ... Cardiac perfusion with DiIC18(5)-DS (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine-5,5’-Disulfonic Acid; Thermo Fisher Scientific) diluted in 4% paraformaldehyde (PFA ...
-
bioRxiv - Bioengineering 2020Quote: Before staining with either 5-bromo-4-chloro-3’-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, ThermoFisher) or Alizarin Red S (ARS ...
-
bioRxiv - Genetics 2019Quote: ... 5’-6FAM-AGC CTA CCT TGA CAA GCA ATC AGA CAC TCA A-MGB-3’ (Life Technologies). ZIKV HD78788 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5’-CAGGAAACAGCTATGAC-3’) and was subsequently recombined with the destination vector using LR Clonase (Thermo Fisher Scientific). The plasmids were then transformed in Agrobacterium tumfaciens strain GV3101.
-
bioRxiv - Cell Biology 2021Quote: ... Sections were washed 3-5 times with TBSTX buffer and then mounted on Superfrost slides (Fisher Scientific) with Slow fade Diamond mounting solution (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... Mouse ENKD1 shRNA (nucleotides targeting 403-423 bp, 5′-CGCTCACCCAAGTATGACAAT-3′) were cloned into pLKO.1 (Invitrogen).
-
bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
-
bioRxiv - Molecular Biology 2020Quote: ... while the EASY-Spray C18 column (Thermo Scientific, 15 cm×75 μm ID, 3 μm, 5 μm) was used as the analytical column ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5’-\56-FAM\ CCT ACC TTA ACC TCC C-3’ with Minor Groove Binder (MGB; ThermoFisher Scientific). PCR was performed using 10X Master Mix to yield a final volume of 25 µl and final working concentrations of 16.6mM (NH4)2SO4 ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections were thawed at room temperature and washed 3 times 5 minutes in DPBS (Gibco, 14190-094). Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL ...
-
bioRxiv - Neuroscience 2022Quote: ... 5′-CCCAAGACTTCCACCTACATTC -3′ (Tm = 62°C) and subsequently subcloned into PCR II-TOPO-TA cloning vector (Invitrogen). Riboprobes were generated with a BIOT-NTP labeling kit (Roche) ...
-
bioRxiv - Microbiology 2022Quote: ... Primers used to amplify the 5’/3’ junction with Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific) are listed in Table S3 ...
-
bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (version v1.5.1, Applied Biosystems). Cq values were obtained via automated threshold determination ...
-
bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... 6-carboxyfluorescein (FAM)-5= CCG TCA ATC AAG GAG CGC CTC 3=-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2021Quote: ... 6-carboxyfluorescein (FAM)-5’ CCG TCA ATC AAG GAG CGC CTC 3’-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
-
bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
-
bioRxiv - Microbiology 2020Quote: ... flanked by 5’ Nhel/3’Apal sites for subcloning into the pcDNA3.1(+) vector (Thermo Fisher Scientific, USA). HEK293T cells and Vero E6 cells were purchased from ATCC ...
-
bioRxiv - Genomics 2022Quote: ... for 3–5 s and then washed thoroughly in phosphate-buffered saline (PBS) (pH 7.4, Gibco, USA). Single zona pellucida-free embryos were carefully pipetted and placed into individual tubes containing 2 μL PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... 2.5 mM probenecid (Life-Technologies: catalog # P36400) and 5 µM Fluo-3 (Life Technologies; catalog # F-14218). Cells were incubated at 37°C for 30 minutes before being centrifuged and resuspended in modified L-15 media containing 2% FBS and 2.5 mM probenecid ...
-
bioRxiv - Cell Biology 2019Quote: ... PKC-specific custom siRNA targeting to endogenous human PKCα (5’-CAGAAGAACTGTATGCAAT-3’) was purchased from Ambien (ThermoFisher). To perform knockdowns ...
-
bioRxiv - Immunology 2020Quote: ... containing a 5′-CACC-3′ sequence required for directional TOPO cloning in pENTR/D-TOPO (Invitrogen, USA), and a reverse primer [“Tgd057 R HA stop” 5’-TTACGCGTAGTCCGGGACGTCGTACGGGTACTCGACCTCAATGTTGTATTC-3’] ...
-
bioRxiv - Microbiology 2021Quote: ... for 3 hours at 37 °C before transforming 5 μL into TOP 10 chemically-competent bacteria (Invitrogen). Mutations were confirmed in selected clones by DNA sequencing ...
-
bioRxiv - Cell Biology 2019Quote: ... actin was incubated with 3-5 molar excess amount of Alexa Fluor 488 C5 maleimide (Invitrogen, #A10254) or EZ-Link Maleimide-PEG2-Biotin (Invitrogen ...
-
bioRxiv - Genomics 2020Quote: ... (RV-5′-GCCGTGGGTACTGAGAAAG-3′) and the fragments were bi-directional sequenced using (3130 Genetic Analyser; Applied Biosystems). Further the results were compared with the reference sequence of ARHGEF40 gene using NCBI-BLAST program and the chromatogram was analysed using Chromas lite (2.1 ...