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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Target preparation was performed using an Eberwine-based amplification method with Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion, AM1753) to generate amino-allyl antisense RNA (aa-aRNA) ...
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bioRxiv - Cell Biology 2021Quote: To perform a bi-parametric analysis of cell cycle based on DNA content (DAPI) and DNA synthesys (EdU) we used the Click-it-EdU Imaging kits (Invitrogen). 0.5 106 mESC were plated on MEF-derived feeders plated on 0.1% gelatin 2 days before the experiment ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was subsequently determined using the fluorescence-based EZQ™ Protein Quantitation Kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Heathy human donor PBMCs or skin fibroblasts were reprogrammed into iPSCs using the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Low passage primary fibroblasts were used to increase reprogramming efficiency ...
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bioRxiv - Microbiology 2019Quote: ... more precise and accurate quantification is performed with sensitive fluorescent-based quantitation assays (“Quant-It” assays kit and QuBit fluorometer, Invitrogen).
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bioRxiv - Cell Biology 2019Quote: Fibroblasts isolated either from human fetal liver tissues or human adult skin after collagenase dissociation were reprogrammed into iPSCs with the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Fibroblasts were used at low population doubling (less than 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... The linearized plasmid was transcribed in vitro based on the procedures of mMessage mMachine T7 Ultra Kit (Thermo Fisher Scientific). DNAse-treated and poly(A)-tailed Cas13d mRNA was purified by using Mega-Clear Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: Cell-cycle experiments were based on the use of the Click-iT Alexa Fluor 647 imaging kit (Thermo Fisher C10340). Reagents were reconstituted according to the user manual ...
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bioRxiv - Neuroscience 2023Quote: ... More precise and accurate concentration estimates were obtained with a fluorescent-based quantitation assay (QuBit fluorometer, “Quant-It” assays kit, Invitrogen). Concentrations were normalized to 2 nM ...
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bioRxiv - Microbiology 2023Quote: Multiplex immunoassay was performed using a Luminex bead-based multiplex ELISA kit (ProcartaPlex Mouse Cytokine & Chemokine Panel 1 26plex, reference # EPXR260-26088-901, Invitrogen). Each sample was normalized to the total protein concentration determined by Bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2023Quote: Blunt-end topoisomerase-based cloning was performed using the PCR products (Zero Blunt TOPO PCR Cloning Kit (ThermoFisher Scientific #K2875J10)) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... From the preculture plate we used 5 μl to inoculate each of four different wells of a 384-well plate (Sigma-Aldrich, Nunc®, flat-bottom) each containing 55 μl of SM containing:
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was examined by 5-bromo-2-deoxyuridine (BrdU) cell proliferation enzyme-linked immunosorbent assay (ELISA) according to the kit manufacturer’s protocol (Life Technologies, Grand Island, NY, USA). The cells were incubated in presence of various concentrations of CLEFMA (0 – 10 µM) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell proliferation was also assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay using Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... 3’ adenine overhangs were added (AmpliTaq DNA Polymerase Kit, Life Technologies). PCR amplification was performed via Kapa Hifi DNA Polymerase (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen) was used according to the manufacturer’s protocol with modest modifications ...
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bioRxiv - Developmental Biology 2019Quote: ... for 3 hrs was isolated using the RNAqueous-Micro Kit (Ambion) and reverse transcribed ...
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bioRxiv - Plant Biology 2019Quote: ... amplified and labeled with GeneChip 3’ IVT Express Kit Assay (Affymetrix). The labeled cDNA was hybridized ...
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bioRxiv - Biochemistry 2023Quote: Pierce™ RNA 3’ Ed Biotinylation Kit (ThermoFisher, cat. no. 20160) was used to label the 3’ end of 1-kb RNA duplexes (produced as described above ...
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bioRxiv - Plant Biology 2023Quote: ... and scanning were performed using the 3¢ IVT Express Kit (Affymetrix) and GeneChip Arabidopsis Genome ATH1 Array (Affymetrix ...
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bioRxiv - Molecular Biology 2023Quote: ... For library generation the Collibri 3’-mRNA Prep Kit (ThermoFisher, A38110024) was used ...
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bioRxiv - Immunology 2020Quote: ... After the fourth EDTA incubation the pieces were cut into 2 mm2 pieces and placed in 5 mL digestion solution containing 5% fetal bovine serum (Gibco), 10 mM HEPES (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples (5 μl) were injected on a C18 PepMap trap column (5 μm, 100 μm I.D. x 2 cm, Thermo Scientific) at 10 μl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Samples (5 μl) were injected on a C18 PepMap trap column (5 μm, 100 μm I.D. x 2 cm, Thermo Scientific) at 20 μl/min ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated for 2-24hrs at 37°C 5% CO2 +/- myristoylated aPKC pseudosubstrate inhibitor (5 μM; Invitrogen; Product number 77749) in IMDM+0.1% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... 1/500.Detection by enhanced chemiluminescence (ECL plus western blotting detection reagents, Thermo Scientific) was performed after incubation with appropriate HRP-coupled secondary antibodies (1:4000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotin detection was performed using the Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific 89880) and imaged with the Omega Lum G (GelCompany ...
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bioRxiv - Physiology 2023Quote: ... Secondary detection was performed with Clean- Blot IP detection reagent (ThermoFisher, 21230, 1:1500).
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bioRxiv - Microbiology 2020Quote: ... Terminal sequences were obtained using 5’ RACE Kit (ThermoFisher) and an anchored oligo-dT primer for the 3’end ...
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bioRxiv - Plant Biology 2023Quote: 5’RACE was performed using the GeneRacer kit (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL Quantitect SYBR Green PCR Kit (Thermo Fisher), and RNA/Nuclease free water ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were visualized using the Pierce ECL 2 Western Blotting Substrate detection method (0.5 pg, lower limit sensitivity; Thermo Scientific). The images were obtained and analyzed with ChemiDoc™ Touch Imaging System (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... 2.6 μL nuclease-free water and 5 μL cDNA template on the ABI Prism 7500 Fast Sequence Detection System (Life Technologies). Thermo-cycling conditions were as follows ...
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bioRxiv - Microbiology 2022Quote: The reverse transcription quantitative PCR (RT-qPCR) assays for the detection of TMPRSS2 were performed using the Quantstudio 5 Real-time PCR system (Applied Biosystems). Premixes were prepared for each amplification reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... real-time PCR of RNA templates using hybridization probe detection chemistries (e.g., TaqMan® 5’-hydrolysis probes) on ABI7500 (Applied Biosystems) was used ...
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bioRxiv - Immunology 2020Quote: ... washed 5 times in TBS-T at room temperature and signals were detected using West Pico Plus ECL detection reagent (Thermo Scientific). Chemiluminescence was subsequently imaged on a BioRad gel imager.
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bioRxiv - Developmental Biology 2021Quote: ... The sections were washed once for 5 min in PBS and Lab Vision™ MultiVision Polymer Detection System (Thermo Fisher Scientific) was used according to manufacturer’s protocol with the following specifications ...
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bioRxiv - Neuroscience 2023Quote: The incorporation of biotin moieties for downstream detection of nanobody constructs was achieved using a 5-fold molar excess of EZ-link-NHS-PEG4-biotin (#21330, Thermo Fisher) with nanobody constructs in 50mM sodium carbonate buffer ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplification and detection of 5′ tRNA-fMet halves were performed using the TaqMan Universal PCR Master Mix (#4304437, ThermoFisher Scientific) as well as custom primers and probe design to target both 5′ tRNA-fMet halves specifically.
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bioRxiv - Neuroscience 2024Quote: ... and Tris-stock (1x 5min, 50mM Tris, pH = 7.6) and incubated for 5 minutes in ECL solution for chemiluminescent detection (Thermo Fisher Scientific). Proteins were visualized using the BioRad Imaging system with Chemi visualization.
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 2-3 hours at room temperature with 2 µg/ml donkey anti-goat Alexa Fluor 488 (Cat# A21206, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 568 (Cat# A10037 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Immunology 2019Quote: ... and IL-2 concentration was measured using a mouse IL-2 ELISA kit (Invitrogen). For CFSE dilution assays ...
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bioRxiv - Neuroscience 2021Quote: ... Relative quantification of Caprin1 was based on triplicate samples using the 2−ΔΔCt method with eukaryotic 18S rRNA as the endogenous control (Applied Biosystems, Cat. No: 4319413E).
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bioRxiv - Neuroscience 2021Quote: ... The chemiluminescent Western blot detection kit (SuperSignal West Femto Chemiluminescent Substrate) and ATP determination kit were from Thermo Scientific (Rockford, IL, USA). The ELISA Kit for rat/mouse insulin ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptotic cells were analyzed with the Ebioscience™ Annexin V Apoptosis Detection Kit APC according to the kit instructions (Cat. 88-8007-74, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Protein-containing fractions were concentrated to ∼15 mg/ml based on a priori sequence-based extinction coefficients 74 and OD280nm values measured using a Nanodrop spectrophotometer (ThermoFisher, Waltham, MA), and the concentrated protein was immediately flash-frozen in aliquots in liquid nitrogen prior to storage at −80 °C pending use.
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bioRxiv - Cancer Biology 2021Quote: ... using a 10 min loading at 3 µL/min flow rate to a trap column (Acclaim™ PepMap™ 100, 2 cm × 75 µm, 3 µm, 100 Å - ThermoFisher Scientific). The separation was performed on an EASY-Spray™ C18 analytical column (25 cm × 75 µm ...