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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen, Waltham, Massachusetts, USA) made in ddH2O for 30 min at 24 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed with 2% PFA and subsequently transferred to V-bottom plates (ThermoFisher, 249570). Cells were quantified by flow cytometry using the Intellicyt iQue at a volume of 100 ul / sample ...
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bioRxiv - Bioengineering 2021Quote: ... on vitronectin-coated (0.5 μg·cm-2) plates and passaged with TrypLE Express (Thermo Fisher Scientific) twice weekly ...
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bioRxiv - Microbiology 2022Quote: ... discoideum amoebae were seeded (2 × 104 cells) in 96-well culture-treated plates (Thermo Fisher) and cultured in HL-5 medium at 23°C ...
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bioRxiv - Bioengineering 2021Quote: ... 2×105 cells per well were seeded in round-bottom 96-well plates (Fisher Scientific) in RPMI with 10% FBS and 2% penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2×105 cells were seeded per well in a 96-well plate (Thermo Fisher Scientific) in 200 µL of GM to reach full confluency ...
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bioRxiv - Immunology 2022Quote: ... at a 2:1 ratio in a 24 well plate in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... These moulds were prepared in 12-well plates using 2% UltraPure agarose (ThermoFisher Scientific, 16500500) (Figure S8B) ...
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bioRxiv - Cancer Biology 2023Quote: ... All plates included 8 wells of negative control siRNA (Ambion Silencer Select Negative Control #2), 8 wells of cell death control (Qiagen AllStars Positive Control siRNA) ...
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bioRxiv - Biochemistry 2024Quote: ... and the plates were washed with 2 mL of 1x PBS pH 7.4 (10010023, Gibco). The cells were treated with 1 mL of trypsin ...
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bioRxiv - Biochemistry 2024Quote: 384-well plates were coated with 2 µg/ml of streptavidin (Thermo Fisher Scientific, MA), followed by blocking ...
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bioRxiv - Microbiology 2024Quote: ... Detection of antibody antigen complexes was performed using the ECL chemiluminescence detection system (Thermo Scientific) in a chemiluminescence imager (ChemiDoc MP Imaging System ...
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bioRxiv - Immunology 2022Quote: Lung cells collected on day 2 and day 6 post-infection was cultured overnight and supernatants collected were assayed for cytokines and chemokines using the multiplex magnetic bead-based kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was detected by an E-gene based RT-qPCR (27) using the AgPath-ID-One-Step RT-PCR kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were used for in vitro transcription and subsequent column-based purification using the MEGAscript RNAi kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... Protein concentration of the extracts was estimated based on tissue weight or normalized using Pierce Coomassie Protein Assay Kit (ThermoFisher Scientific). Co-immunoprecipitation (coIP ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions) of genomic DNA (∼1000x shRNA representation) to PCR based barcoding and amplification with AmpliTaq Gold PCR kit (ThermoFisher Scientific). See Supplementary Table 3 for list of barcode adaptor primers ...
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bioRxiv - Developmental Biology 2022Quote: ... 6d ovaries underwent 1h of EdU labelling based on the protocol of the Click-iT™ Plus EdU Cell Proliferation Kit for Imaging (Invitrogen). Both 6d and 12d ovaries were stained for Fasciclin III (Fas3 ...
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bioRxiv - Microbiology 2020Quote: ... The copy number of AEFV RNA was determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) based on serial dilution of the AEFV NS4 RNA with Maxima H minus First Strand cDNA Synthesis kit (Thermo Fisher) and Fast SYBR Green Master Mix (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Purified small RNA sequencing libraries were quantified using the fluorescent dye-based Qubit ds DNA HS Assay Kit (Thermo Fisher Scientific) on a 2100 Bioanalyzer ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were diluted 1:100 in extraction buffer before quantification using a luciferase-based ATP kit (A22066, Thermo Fisher Scientific). Values were expressed relative to total protein concentration measured by using a BCA assay (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... The ATP content from 10 μL aliquots of lysed cells was then detected using a luminescence-based ATP determination kit from Life Technologies and ATP levels were calculated by extrapolation from a calibration curve constructed with known concentrations of ATP ...
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bioRxiv - Molecular Biology 2020Quote: ... cells with already detected EdU were resuspended in 400 μl 1x Click-iT saponin-based permeabilization and wash reagent from the Click-iT Plus EdU Alexa Flour 647 Flow Cytometry Assay Kit (Thermo Scientific). Subsequently ...
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bioRxiv - Genomics 2021Quote: ... Normalization and pooling of the enriched libraries were based on quantification using the Quant-iT dsDNA kit (Life Technologies, ON, Canada). Molar quantification of the pooled library was performed using GeneRead Library Quant Kit for Illumina (Qiagen Canada ...
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bioRxiv - Synthetic Biology 2020Quote: ... The vector and the 12-mer were joined using the homologous recombination-based method (GENEART Seamless Cloning and Assembly Kit, ThermoFisher Scientific). The resulting plasmid carries one copy of α21-IILacO/Gal4 12-mer accompanying unique NheI and unique SpeI site at the ends.
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bioRxiv - Microbiology 2020Quote: ... Total intracellular or cytoplasmic HCMV DNA was quantified with the UL83-based qPCR after extracting viral and cellular DNA from infected cells with the PureLink Genomic DNA mini kit (Thermo Scientific). We also measured the chromosomal DNA molecules present in these samples with an amplicon located in the human beta-2 microglobulin gene (80) ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was subjected to DNase treatment and magnetic bead-based mRNA enrichment using the Dynabeads™ mRNA Purification kit (Invitrogen™ ...
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bioRxiv - Developmental Biology 2020Quote: ... 6d ovaries underwent 1h of EdU labelling based on the protocol of the Click-iT™ Plus EdU Cell Proliferation Kit for Imaging (Invitrogen). Both 6d and 12d ovaries were stained for Fasciclin III (Fas3 ...
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bioRxiv - Microbiology 2023Quote: ... ModB R73A and rS1 mutants were generated by site-directed mutagenesis using a procedure based on the Phusion Site-Directed Mutagenesis Kit (Thermo Scientific). The resulting plasmids were sequenced and transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... according to manufacturer’s instructions and quantified using the fluorescent dye-based Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA). Samples were equimolarly pooled and sequenced using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used the Qubit dsDNA HS Assay Kit to quantify DNA concentrations based on the standard protocol on a Qubit 3.0 fluorometer (Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2023Quote: ... and pooled with similar molecular numbers based on measurements made with a Qubit dsDNA High Sensitivity kit (Thermo Fisher Scientific, Q32854) and a plate reader (Eppendorf ...
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bioRxiv - Molecular Biology 2023Quote: Cartilage explants viability before and after treatment with CHIR99021 was measured using the resazurin-based PrestoBlue™ Cell Viability Reagent kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Purified amplicons were then pooled based on the concentrations measured by the Quant-iT™ dsDNA Assay HS Kit (Thermo Fisher) and sequenced using Amplicon-EZ (Genewiz).
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bioRxiv - Biochemistry 2020Quote: ... the cells were passaged 3 to 4 times in DMEM based media with same supplements as above but with HEPES based CO2-independent buffering (5958 mg/L) (Thermo Fisher Scientific, 21063045) instead of phenol red.
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bioRxiv - Cell Biology 2021Quote: ... This served as the H2O-based media for experiments and the D2O-based media was made to an identical composition using RPMI media powder (Thermo Fisher Scientific, #31800022) and heavy water (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies, Darmstadt, Germany). Fold expression changes were determined using a standard 2−ΔΔCT method that compares CT (cycle threshold ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Biophysics 2022Quote: ... and 2-3 μL of the freshly phase-separated sample was placed into a chamber made on a glass slide (Fisher Scientific 3” × 1” × 1 mm). The chamber made by using double-sided tape was then sealed with a square coverslip to avoid evaporation of the sample ...
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bioRxiv - Immunology 2021Quote: ... PCR amplification and detection of 5′ tRNA-fMet halves were performed using the TaqMan Universal PCR Master Mix (#4304437, Thermo Fisher Scientific) as well as custom primers and probe design to target both 5′ tRNA-fMet halves specifically.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
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bioRxiv - Physiology 2022Quote: ... Expression of the various genes was determined by the comparative CT method (ABI Prism 7700 Sequence Detection System User Bulletin #2, Applied Biosystems). Primer sequences of dFOXO were as follows ...
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bioRxiv - Genetics 2021Quote: ... Expression of the various genes was determined by the comparative CT method (ABI Prism 7700 Sequence Detection System User Bulletin #2, Applied Biosystems). Primer sequences of PGC-p/_ were as follows ...
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bioRxiv - Immunology 2024Quote: ... and the cells were fixed for 10 minutes in 2% Formaldehyde at room temperature before detection on the Attune NxT Flow Cytometer (ThermoFisher Scientific). FCS files were analyzed using FlowJo v10.
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bioRxiv - Immunology 2021Quote: The IL-2 ELISA was performed using the IL-2 Human ELISA Kit by ThermoFisher scientific according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... and biotinylated using the Biotin 3′ End DNA Labeling Kit (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: Oligonucleotides were labelled using a 3’ biotin end-labelling kit (Thermo Scientific). Binding reactions were carried out at room temperature with 1µg of GBP2 in the presence of 50ng dIdC ...
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bioRxiv - Immunology 2020Quote: ... A beta release of the Collibri 3’ mRNA Library Prep Kit (Invitrogen) was used to prepare libraries ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was labelled using the 3’ end desthiobiotinylation RNA labelling kit (ThermoFisher), with 150ug/25nM RNA per reaction incubated at 16°C overnight ...
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bioRxiv - Immunology 2020Quote: ... kit in a QuantStudio™ 3 Reak-Time PCR Instrument (Applied Biosystems). The ΔΔCt method was used to analyze levels of transcripts and data were normalized to the levels of Gapdh or as indicated.