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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cell lines were transfected with pOG44 and pcDNA5/FRT/TO plasmids harboring respective FLAG constructs using Lipofectamine3000 (Invitrogen) as transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney line 293 (HEK293) wildtype and HTT-null cells were grown in Dulbecco’s modified Eagle’s medium (Thermofisher #41965) supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher #10500064) ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI- cells were grown to approximately 90 % confluency on 24-well tissue culture plates (cat# 11391704, Fisher Scientific) before the medium was exchanged for serum reduced DMEM and the transfection mixture was added ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were generated by integrase-mediated homologous recombination of the CFTR sequence into the T-REx HEK293 background (ThermoFisher Scientific). This expression system allows the tetracycline-inducible expression of genes of interest from one defined insertion site ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Parental T-REx-HEK293 cells were cultivated in growth medium: Dulbecco’s modified Eagle’s medium (DMEM) + GlutaMAX-I (#31966, Life Technologies), 10% Dialyzed FBS (Gibco ...
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bioRxiv - Bioengineering 2022Quote: Flasks of HEK293 cells were pre-labelled red or green using cell tracker labels (C7025 and C34552, Thermofisher Scientific) or blue using Hoescht 33342 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... All cell lines were maintained in HEK293 culture medium – Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate; Gibco, #41966029) with 10% foetal calf serum (FCS ...
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Synthetic Biology 2023Quote: ... Hek293-T cell lines were cultured in DMEM (ATCC; 30-2002) supplemented with 10% FBS (Thermo Fisher Scientific; 10082147), 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and ATG13 KO GFP-LC3B HEK293 cells (a kind gift from O. Florey) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO Life Technologies) containing 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Cell Biology 2023Quote: ... wild type HEK293 or HEK293A (for figure 1A) cells were transfected with the indicated plasmids with Lipofectamine2000 Reagent (Invitrogen) as per manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: NIH3T3 derived Bmal1-luc31 and HEK293/T17 cells were cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Gibco: Cat #11965118) supplemented with 10 % fetal bovine serum (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... Stable cell lines were made by transfecting HEK293 cells with the respective plasmids using Lipofectamine 2000 (Invitrogen, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium 5 g/L glucose (DMEM; Gibco, UK, 11965084). Cell culture media were supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Polymerase chain reaction (PCR) was conducted using a Veriti 96-well Thermal Cycler (Applied Biosystems, Foster City, USA) following the protocols described in Barfuss et al ...
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bioRxiv - Plant Biology 2022Quote: ... DNA amplifications were performed by polymerase chain reaction (PCR) using the Phusion high-fidelity DNA polymerase (Thermo Scientific). PCR products of the appropriate size were electrophoretically separated in 1% agarose gels and recovered by elution ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the heavy and light chains were co-transfected into FreeStyle™ CHO-S™ cells (Invitrogen) in serum free FreeStyle™ CHO™ Expression medium ...
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bioRxiv - Genomics 2020Quote: ... Real-time quantitative polymerase chain reaction (PCR) was performed on QuantStudio 5 Real-Time PCR Instrument (Applied Biosystems). All experiments were done in triplicate with SYBR-GreenER pPCR SuperMix (Life Technologies) ...
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bioRxiv - Physiology 2019Quote: ... Real-time quantitative polymerase chain reaction (RT-qPCR) was performed on a StepOnePlus RT-qPCR machine (Applied Biosystems) with each reaction consisting of triplicate samples containing iTaq Universal Probes Supermix (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... The antibody variable heavy and light chain variable regions were PCR amplified using AmpliTaq360 Master Mix (Applied Biosystems). PCR products were purified (Qiagen ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... or a long-chain fatty acid (Hexadecanoic Acid, C16; BODIPY™ FL C16, D3821, Thermo Fisher Scientific Inc.) were added at a concentration of 4 µM to the culture medium as defined by culture mode (table 3) ...
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bioRxiv - Cell Biology 2021Quote: The primary antibodies used were mouse anti-clathrin light chain (AB CON.1, MA5-11860, Thermo Fisher Scientific), mouse anti-clathrin heavy chain (AB X-22 ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using PowerUp SYBR Green master mix (Applied biosystems, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using PowerUp SYBR Green master mix (Applied biosystems, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The Fab heavy and light chains of AI774 and AI878 were individually cloned into a pcDNA3 vector (Invitrogen). A 6x-His tag was added to the C-terminus of each Fab heavy chain sequence ...
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bioRxiv - Microbiology 2022Quote: ... The polymerase chain reaction (PCR) was performed as described previously (60) using Pfu turbo polymerase (Invitrogen, Burlington, ON). Site directed mutagenesis was performed using the QuikChange method (Stratagene Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time reverse transcriptase polymerase chain reaction (qPCR) was performed using SYBR Green (Thermo Scientific, #4309155 Germany). Primer sequences are listed in Supplementary Table S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The quantitative polymerase chain reaction was performed using the Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... Expi293 cells were transfected with plasmids encoding Ig heavy and light chain pairs with Expi-Fectamine (ThermoFisher Scientific) and hmAbs were purified from the culture supernatants using Sepharose Protein A (Pierce).
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bioRxiv - Microbiology 2022Quote: ... quantitative polymerase chain reaction (qPCR) was performed using TaqMan Fast virus 1 Step PCR Master Mix (Applied Biosystems); a standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative Polymerase Chain Reaction (qPCR) was performed in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using SYBR Green Real-Time PCR Master mix (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: Gene expression was analysed via reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using TaqMan probes (Life Technologies) and SSO Universal Probes Master Mix (Bio-Rad) ...
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bioRxiv - Physiology 2019Quote: All polymerase chain reactions (PCRs) were carried out in ABI StepOnePlus Real-Time PCR Systems (Applied Biosystems, USA) using the RealQ Plus 2x Master Mix Green Kit (Ampliqon ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using PowerUp™ SYBR™ green (Life technologies) and assayed for genes associated with stages of development and eventual cell types (Chondrogenic ...
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bioRxiv - Physiology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using Power SYBR Green Master Mix (Life Technologies) on a QuantStudio 12K Flex real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative polymerase chain reaction (qPCR) assessed RNA amount using a StepOnePlusTM Real-Time PCR System (Thermo-Fisher Scientific) relative to the internal control of 18s ribosomal RNA (18s rRNA) ...
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bioRxiv - Microbiology 2020Quote: ... Real-time polymerase chain reactions (qPCR) were performed using the Ambion Ag-Path master mix (Life Technologies, USA) and the ABI 7500 instrument ...
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bioRxiv - Cell Biology 2020Quote: ... Polymerase chain reactions (PCR) for cloning were carried out with Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific). An initial selection of positive transformants by colony PCR was carried out using Phire polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Heavy and light chain plasmids for each monoclonal antibody were co-transfected into Expi293F cells (Thermo Fisher Scientific). After incubating for 5 days at 37°C with 8% CO2 ...
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bioRxiv - Genomics 2022Quote: ... Quantitative real-time polymerase chain reaction (RT-qPCR) was performed using Fast SYBR Green Master Mix (Applied Biosystems) and custom intron-spanning primers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2023Quote: DNA sequences coding for full-length TCR chains were codon optimized and synthesized by GeneArt (Thermo Fisher Scientific) or Telesis Bio as strings ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The codon-optimized for CHO variable light and heavy chains (02B11, atezolizumab, and durvalumab) were synthesized (ThermoFisher Scientific) and then cloned into pTRIOZ-mIgG2a/mkappa (κ ...