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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Western blots were carried out probed with anti-His mAb (1:1000, Invitrogen) followed by anti-mice IgG-HRP (1:20000 ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed using anti-His antibody (Thermo Fisher, Cat. No. MA1-21315) at a dilution of 1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... neutralized by adding RPMI-1640 with 10% heat inactivated FBS (HI FBS, Gibco), and spun down at 200 x g for 10 minutes to pellet the cells ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Synthetic Biology 2024Quote: ... then diluted at 1:100 in Hi-Def Azure Media (Fisher Scientific # 3H5000) supplemented with 1% Glucose and various concentrations of peptide in 100 µl in a 96well plate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were placed in HI buffer containing 1:1,000 CellMask Deep Red (Invitrogen) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... excluding the HI-FBS and supplementing with 0.1% BSA (Life Technologies 15260-037). CHIM was used for the experiments in Figures 4 ...
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bioRxiv - Microbiology 2024Quote: ... Bio-His-FIKK13 was incubated with different quantities of magnetic streptavidin beads (Invitrogen) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Cancer Biology 2021Quote: ... and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, California, USA) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration ...
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bioRxiv - Cell Biology 2019Quote: ... In immunofluorescence and western blot study we used the following antibodies: Beta-actin (Thermofisher cat no. MA515739) Beta Tubulin (BT7R ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with primary Cholera Toxin beta polyclonal antibody (rabbit; 1:1000, RRID: AB_779810, Invitrogen) diluted in PBS-T and for approximately 1h30 with donkey antirabbit secondary antibody AlexaFluor647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... In immunofluorescence and western blot study we used the following antibodies: Beta-actin (Thermofisher cat no. MA515739), Anti Alpha Tubulin Antibody Clone DM1A (Thermofisher cat no-62204) ...
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bioRxiv - Cancer Biology 2019Quote: ... 25ng cDNA was amplified using the Oncomine TCR Beta-LR Assay (Thermo Fisher Scientific Cat. No. A35386) and protocol as described in the Oncomine TCR Beta Assay User Guide MAN0017438 Revision A.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Flour 594 Cholera toxin beta subunit conjugate (CTb-594, Invitrogen; 0.5% w/v, 400–500 nl) was injected in dHPC of CD-1 mice ...
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bioRxiv - Biochemistry 2023Quote: ... Membrane defects are blocked for 15 minutes with a 1 mg/mL beta casein (ThermoFisher, Cat# 37528) diluted in 1x PBS [pH 7.4] ...
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bioRxiv - Biochemistry 2023Quote: ... Membrane defects are blocked for 10 minutes with a 1 mg/mL beta casein (ThermoFisher, Cat# 37528) diluted in 1x PBS [pH 7.4] ...
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bioRxiv - Cell Biology 2022Quote: ... and nuclear beta-galactosidase (nβGal) were synthesized with linearized templates using SP6 polymerase (Ambion mMessage mMachine Kit). All experimental procedures were performed according to USDA Animal Welfare Act Regulations and have been approved by Institutional Animal Care and Use Committee ...
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bioRxiv - Immunology 2023Quote: IL-1β secretion was detected by IL-1 beta Mouse Uncoated ELISA Kit (88-7013-88; Invitrogen) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... Human PAR4 containing an N-terminal V5-epitope were stably expressed using the HEK293 Flp-In T-Rex cells according to the manufacturer’s protocol (Invitrogen). The concentration of tetracycline was titrated (700 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.
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bioRxiv - Molecular Biology 2020Quote: ... GFP-N was immunoprecipitated from two independent HEK293 cell pellets using 5μg of anti-GFP antibody (Life Technologies G10362). A total of 2% input material was obtained for size-matched control libraries (SMI ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (Invitrogen catalogue number R780-07). Cell cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Genetics 2019Quote: HEK293 cells (CRL-1573, ATCC, VA, USA) were cultured in high-glucose DMEM (Cat. No. 11965092, Life Technologies, USA) supplemented with 10% FBS at 37 and 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... The HEK293 cells were transfected with 100 ng/well of pGL3-Fluc plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol at around 75-90% confluency in a 96 well plate ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides from HEK293 and HeLa cells were respectively injected into LTQ-Orbitrap Velos and Q Exactive (Thermo Fisher) onto a 75 μm i.d ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney (HEK293) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L Glucose and GlutaMAX™ (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with GFP constructs (250 ng/well) for 24 (HEK293) or 48 (SH-SY5Y) hours using Lipofectamine 3000 (0.75 µL/well; Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells, HEK293 or MEF cells were cultured in 10% FBS (Hyclone, SH3007103) in high glucose DMEM (Gibco 11965118) with 1% penicillin/streptomycin at 37°C ...
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bioRxiv - Immunology 2021Quote: HEK293 freestyle cells were transfected with this expression construct in combination with pBu-BirA using 293Fectin (Thermo fisher scientific) transfection reagent at 1μg DNA/10^6 cells ...
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bioRxiv - Neuroscience 2022Quote: ... which were used to directly transfect HEK293-Kir2.1 cells with the linear product using lipofectamine 3000 (Thermo Fisher Scientific). For patch clamp characterization in HEK293A cells ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293E or HEK293-6E cells were maintained in suspension cultures in Freestyle 293 Expression Media (Gibco, Waltham, MA, USA) supplemented with heat-inactivated fetal calf serum (1% ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: Cells (primary neurons or HEK293 cells) were transfected with 1.6 µg/mL of a pIRES2-EGFP plasmid vector (Invitrogen) harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number ...
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bioRxiv - Immunology 2019Quote: ... we performed immunoprecipitation of CT-DNA stimulated HEK293 cells using the antibody to IRF3 pS386 crosslinked to Dynabeads (ThermoFisher) overnight at 4 C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T and Flp-In HEK293 T-REx were maintained in DMEM-GlutaMAX at 4.5 g*ml-1 D-glucose (Gibco) supplied with sodium-pyruvate ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting vector was transiently transfected into HEK293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific # A14525). After enhancement ...
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bioRxiv - Neuroscience 2020Quote: ... were subcloned into the polylinker of the pcDNA3.1 vector (Invitrogene, USA) by standard techniques for transiently transfecting HEK293-F cells (ThermoFisher). Cells were transiently transfected with the plasmids containing the desired GABAAR subunit cDNAs (α ...