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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: Adherent HEK293 cell lines were grown in 10 mLs of DMEM (ATCC 302002) containing 10% FBS (Gibco 16000044) and 1% Pen/Strep (Gibco 15070063) ...
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bioRxiv - Microbiology 2022Quote: ... as well as the HEK293 cell line were grown in Dulbecco’s Modified Eagle’s Medium F-12 50/50 (DMEM/F-12, GIBCO) supplemented with 10% FBS at 37°C with 5% CO2 in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentivirus production was performed according to the manufacturer’s recommendations in HEK293 E cells using Lipofectamine 3000 (ThermoFisher Scientific). Virus-containing supernatant was collected after 24 and 48 h and combined ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Stable HEK293 cell lines were achieved by antibiotic selection with G-418 (1mg/ml, Gibco-BRL, Paisley, UK) added to the medium 3 days after transfection ...
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bioRxiv - Bioengineering 2023Quote: ... which then were co-transfected into HEK293 6E cells for expression together following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-6E cells (used for protein expression) were cultured in expression medium (FreeStyle F17 (Gibco, Cat#A13835-01)) supplemented with 1% FBS (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells were transiently transfected with 1 µg of each cDNA using Lipofectamine 2000 (Invitrogen, Carlsbad CA, USA). After 12 h of transfection ...
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bioRxiv - Genomics 2023Quote: ... HEK293 cells and MCF7 cells purchased from ATCC were cultured in DMEM (high glucose, Life Technologies Corporation, 11965084) containing 15% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: Hek293T and Hek293 Flp-In T-REx cells were maintained without antibiotics in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... HEK293 cells were seeded and grown on poly-lysine-coated Lab-Tek II eight-well chamber slides (NUNC) overnight and transfected with plasmid DNA using the Lipofectamine™ 3000 Transfection System (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: Lentivirus was made by transfecting HEK293 cells with ViraPower packaging plasmids (pLP1, pLP2, and pLP/VSVG) from Invitrogen along with the viral vector using JetPrime ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 105 HEK293 cells were seeded in flat-bottom 24-well plates (Thermo Fisher Scientific or FALCON) and incubated overnight ...
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bioRxiv - Genomics 2023Quote: ... sgRNA-transduced HEK293-idCas9 cells were cultured in high glucose DMEM medium supplemented with L-Glutamine (Gibco 11965092) and 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293) cells obtained from ATCC (Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK293 cells were cultured in a 2L suspension using FreeStyle 293 Expression Medium (Life Technologies, Carlsbad, CA, USA) with a cell density maintained at 1 × 10^6 cells/mL on the day of transfection ...
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bioRxiv - Microbiology 2024Quote: ... HEK293 ACE2 and lung carcinoma epithelial cells A549 ACE2 were cultivated with Dulbecco’s Modified Eagle Medium (DMEM; Gibco) with 10% fetal bovine serum (Sigma-Alrich ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC CRL-1573) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Paneco) supplemented with glutamine (Gibco), 10% fetal bovine serum (HyClone) ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Immunology 2022Quote: ... The resulting plasmids of heavy and light chain pairs were co-transfected in Expi293F cells (Thermo Fisher) using Turbo293 transfection reagent (Speed BioSystems ...
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bioRxiv - Bioengineering 2021Quote: ... TaqMan™ reagents and primers for real-time polymerase chain reaction (PCR) were purchased from Applied Biosystems by Thermo Fisher (Waltham ...
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bioRxiv - Synthetic Biology 2021Quote: ... amplified via polymerase chain reaction (PCR) with Phusion High-Fidelity PCR Master Mix (Cat #: F531L) from ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding Ig heavy and light chain pairs were co-transfected using Polyethylenimine into 293FreeStyle cells (Invitrogen). Supernatants were assayed for secreted rAb and for HA reactivity using the HA CBA described above ...
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bioRxiv - Microbiology 2021Quote: Quantitative polymerase chain reaction (qPCR) was completed on a QuantStudio™ 6 Flex System (Applied Biosystems™) with samples prepared on a 384-well (0.2 mL ...
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bioRxiv - Cell Biology 2021Quote: ... ARPE-1 (RPE) cells stably expressing clathrin light chain-eGFP were grown in DMEM/F12 medium (Gibco) supplemented with 10% FBS [74] ...
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bioRxiv - Microbiology 2022Quote: Polymerase chain reactions (PCR) for local DNA sequencing were performed using 2x PCR Master mix (Thermo Scientific). The following PCR program was used ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and infection status was determined using a polymerase chain reaction (PCR) assay (Simpliamp ThermoCycler, Applied Biosystems, Singapore). We amplified the Wolbachia-specific wsp gene (Forward ...
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bioRxiv - Physiology 2020Quote: ... and the nucleotide chains were extracted from the gel using the GeneJET Gel Extraction Kit (Thermo Scientific). Overhang adenines were added to the 3′-ends of PCR products using Dynazyme II DNA polymerase (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time quantitative polymerase chain reaction (qPCR) was performed using Fast SYBR Green Master Mix (Life Technologies) on QuantStudio 7 Flex system (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed using Fast TaqMan Master Mix (Applied Biosystems, #44456) with an Applied Biosystems 7900HT Fast Real-Time PCR System ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were transfected into 96-well cultures of ExpiCHO cells (ThermoFisher Scientific, A29127) for microscale expression ...
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bioRxiv - Immunology 2021Quote: DNA constructs encoding anti-SCARB2 antibody heavy and light chain variable domains (clone JL278) were synthesized (ThermoFisher) and cloned into mammalian expression vectors containing a mouse IGHV signal peptide and IgG1 constant regions ...
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bioRxiv - Immunology 2021Quote: ... The constant regions of heavy (HC) and light chain (LC) were cloned separately into pcDNA3.4 (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... Real-time quantitative polymerase chain reaction (qPCR) was performed using PowerTrack SYBR Green master mix (Life Technologies). Equivalently diluted mRNA without the qScript cDNA mix were used as negative controls ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative polymerase chain reaction (qPCR) was done using TaqMan™ Fast Advanced Master Mix (Thermo Fisher Scientific) and the real time-PCR machine (ABI7500fast ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were transfected with plasmids encoding Ig heavy and light chain pairs with ExpiFectamine (ThermoFisher Scientific). Monoclonal antibodies were purified from the cell supernatant using sepharose Protein A (Pierce).
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bioRxiv - Plant Biology 2023Quote: ... Yeast colony polymerase chain reaction (PCR) was done using the Phire polymerase following manufacturer’s instructions (Thermo Fisher).
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bioRxiv - Plant Biology 2023Quote: ... and Alexa Fluor 647 goat anti-rat IgM (μ chain) were purchased from Invitrogen (Eugene, OR, USA).
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bioRxiv - Immunology 2023Quote: ... Quantitative polymerase chain reaction (qPCR) was then performed using Power SYBR Green PCR Master Mix (ThermoFisher Scientific) on a CFX384 Real-Time PCR detection system (Biorad ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using Taqman PCR Master Mix (Applied Biosystems) and samples were amplified on an ABI Prism 7900HT Fast Detection System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time quantitative polymerase chain reaction (qPCR) was performed using PowerTrack SYBR Green master mix (Life Technologies) or iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Emulsion polymerase chain reaction (PCR) based target enrichment was performed using Ion OneTouch 2 system (ThermoFisher Scientific). Enriched libraries were sequenced using an Ion Torrent Personal Genome Machine (PGM ...
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bioRxiv - Biochemistry 2023Quote: Thermal shift assays were performed with a StepOnePlus real-time polymerase chain reaction (PCR) machine (Life Technologies) using SYPRO Orange dye (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... reverse transcription polymerase chain reaction (RT-PCR) was performed utilizing SuperScript III (Thermo Fisher, Waltham, MA, USA). The reverse transcription process occurred at 58 °C for 50 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Polymerase chain reaction (PCR) was conducted with the Taqman® Fast Universal PCR Master Mix (Applied Biosystems). The TaqMan® probes and primers that were used are as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Respiratory chain complexes and supercomplexes were separated on Native PAGE Novex 3%–12% Bis-Tris gels (Invitrogen) for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Polymerase chain reactions (PCR) for cloning were carried out with Phusion High-Fidelity DNA Polymerase (Thermo Scientific). Colony PCR was carried out using Phire polymerase (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... and were transiently transfected with an equimolar plasmid mixture of heavy and light chain using Expifectamine (Invitrogen). For a typical 100mL size transfection 100µg amount of total DNA and 270µL of lipofectamine were used ...
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bioRxiv - Immunology 2024Quote: pCMV-mCD8 carries the mouse CD8 α-chain driven by the CMV immediate early promotor/enhancer (Invitrogen). The Adenoviral expression vectors ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...