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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/mL, Invitrogen) for 5 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation with 2 μM calcein-AM and 4 μM ethidium homodimer-1 (Invitrogen) for 30 min to examine viability ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (D1306, 1:10,000, Invitrogen) diluted in 1X PBST (1:10,000) ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 4′,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were then stained with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, D1306, 1:5000) for five minutes to visualize DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:3000; #D3571, Invitrogen) then mounted on glass slides with Mowiol ...
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bioRxiv - Bioengineering 2022Quote: ... stained with 5 μM DRAQ5 (Thermo Scientific, nuclear dye, 5 minutes at 37°C), fixed in 4% paraformaldehyde for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 2 weeks of selection with 3 μg mL−1 of puromycin (Thermo Fisher Scientific). Then ...
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bioRxiv - Biophysics 2020Quote: ... hiPSCs were dissociated by incubating at 37°C for 7 minutes with TrypLE™ Express (ThermoFisher) and seeded at 125000 cells/cm2 on a Matrigel® coated 12 well plate ...
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bioRxiv - Cancer Biology 2020Quote: ... and finally at 72 °C for 7 min using AmpliTaq Gold PCR Master Mix (Applied Biosystems) with template cDNA equivalent to 15 ng of total RNA and a high concentration (0.75 mM each ...
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bioRxiv - Neuroscience 2024Quote: ... for 7 min at 37°C and the digestion was blocked with 10% FBS (#10500064, Gibco) in DMEM (#41966-029 ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Molecular Biology 2023Quote: ... and GlutaMAXTM at 37°C with 5% CO2 (Gibco). XP2YO ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Rev: 5’-TCATTGAGACACCATTTGTC-3’ were cloned into pCR™4-TOPO® TA vector using the TOPO-TA cloning kit (Thermo Fisher 450030) and sequence verified.
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bioRxiv - Genetics 2021Quote: ... at 85 V for 2 hours 15 minutes at an ambient temperature of 4 °C in NuPAGE™ Transfer Buffer (Invitrogen, UK) containing 10% 2-propanol (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Embryo hippocampi were dissected in 4 °C Hibernate E supplemented with 2% B27 supplements and 100 U/mL Penicillin/Strep (Thermo Fisher Scientific). Hippocampal tissues were digested in Hibernate E containing 20 U/mL papain ...
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bioRxiv - Cancer Biology 2019Quote: ... washed extensively with phenol red-free RPMI containing 2% FBS and stored overnight at 4°C prior to staining with CellEvent Senescence Green (Thermo Fisher Scientific). Flow cytometric analysis and sorting was performed at the Flow Cytometry shared Resource Facility at the Icahn School of Medicine at Mount Sinai ...
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bioRxiv - Biochemistry 2021Quote: ... Grids were then blotted with filter paper for 2 s at 100 % humidity at 4 °C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: The ubiquitylated worm CMG was then incubated for 30 minutes at 4°C with 2 µl (per 10 µl ubiquitylation reaction mix) magnetic beads (Dynabeads M-270 Epoxy; Life Technologies, 14302D) that had been coupled to anti-worm SLD-5 antibodies ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Microbiology 2021Quote: Three µL of purified virus particles were loaded on a glow-charged lacey carbon copper grid and flash frozen in liquid ethane after 2 s blotting in 100% humidity at 4°C using a Vitrobot Mark IV (Thermo Fisher Scientific). The particles images were collected using a 300 kV Titan Krios G2 microscope (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The intracellular Ca2+ concentration was assayed for 20 min at 37°C with 2 µM Fluo-4-AM (catalog number F14201, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and treated during 30 minutes at 37°C with 4 µg of RNAse and 2 U of Turbo DNAse (Ambion, Ref AM2239). Another incubation of 30 minutes at 37°C with an additional quantity of Turbo DNAse (2 U ...
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bioRxiv - Biochemistry 2024Quote: ... Quantifoil R2/2 200-mesh copper grids with 2 nm carbon (EM sciences) at 4 °C and 100% humidity using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). The sample was blotted for 5s ...
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bioRxiv - Neuroscience 2024Quote: ... Joachim Herz) for 2 h at 4°C followed by overnight incubation with 20 μL of protein G Ultralink resin (ThermoFisher Cat# 53128). The resins were washed with immunoprecipitation buffer and precipitated proteins were eluted with reducing SDS sample buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemistry was performed by incubating with primary antibodies (overnight, 4°C) and fluorophore-conjugated secondary antibodies (2 hr, room temperature; ThermoFisher, Jackson ImmunoResearch). Sections were mounted with VectaShield (VECTOR ...
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bioRxiv - Microbiology 2023Quote: ... David Steinhauer) at 4 °C overnight and 2 µg/ml AlexaFluor647 Donkey anti-rabbit IgG secondary antibody (ThermoFisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed with 6 mL Accutase for 10 sec and incubated in 4 mL TrypLE select for 2 min/37°C before addition of 200 μL of Neuronal isolation enzyme (Thermo Scientific, 88285) and further incubating 1 min/37°C ...
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bioRxiv - Biophysics 2023Quote: ... 0.5% NP-40 alternative) with protease inhibitors and cleared for 2 hours at 4 °C with magnetic Protein G Dynabeads (Thermo Fisher, #10009D) before overnight immunoprecipitation with 1 ug per 250ug of proteins of either normal serum IgGs or specific antibodies as listed above ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were blotted for 2 s at 100% humidity at 4 °C and flash frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... Scaffolds were washed in PBS at every timepoint (day 1, 4, 7, 14, 28) and placed in a solution of alamarBlue® (Invitrogen, California, USA) in an incubator at 37°C on a shaker ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of total RNA was estimated using a Nanodrop One C (Thermo Fisher Science) and RNA library formation was performed using the Illumina HiSeq 6000 (Illumina) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The DNA of each sample was quantified in a Nanodrop One C spectrophotometer (Thermo Scientific) and aliquoted at 20 ng/µL in ultra-pure water ...
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bioRxiv - Immunology 2024Quote: ... and one round of positive selection by anti-c-myc antibody conjugated beads (Thermo Fisher) were performed as previously reported [5] ...
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bioRxiv - Bioengineering 2019Quote: ... at 4°C followed by AlexaFluor-568 antibody (1:500) and concurrently with AlexaFluor-647-conjugated bungarotoxin (1:1000, Invitrogen, B35450) for two hours at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... samples were incubated with mouse-anti-Pax7 (1:10, DSHB) overnight at 4°C followed by AlexaFluor-647 antibody (1:500, Invitrogen, A31573) for two hours at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... NB100-2284) overnight at 4°C followed by AlexaFluor-568 antibody (1:500) or AlexaFluor-568 antibody (1:500, Invitrogen, A10087), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... the pooled anion exchange fractions were phosphorylated in vitro overnight at 4 °C with 1 mM ATP and 1 μg of PKR(252-551)-GST enzyme (Thermo Scientific) per mg of eIF2α ...
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bioRxiv - Microbiology 2021Quote: ... antibody 1:3000 in 1% PBS/BSA at 4°C followed by secondary antibody conjugated to Alexa Fluor 555 (Thermo Fisher) at 1:500 in PBS/BSA 1% was performed ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals, 48 hrs at 4°C; Th: anti-Th [rabbit] 1:1000, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (1% BSA/PBS-T, overnight at 4°C) at the following dilutions: mouse anti-SAG1 (1:1000, Thermo Scientific) and rat anti-HA (1:1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Larvae were rinsed in PBST three times for 20 min and incubated overnight at 4°C with secondary antibodies (anti-mouse 1:500 IgG-Alexa Fluor 488, 1:500 IgG Alexa Fluor 594, Molecular Probes; anti-rabbit IgG-Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041; AlexaFluor 647, 1:200, Invitrogen, A21236). Whole muscles were dehydrated in a series of 25% ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:250 dilution overnight at 4°C and anti-mouse AlexaFluor-555 secondary antibody (1:1000, ThermoFisher Scientific, #A-21424) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 1:600) antibody was incubated at 4°C overnight followed by secondary antibody anti-rabbit Alexa 488 (ThermoFisher Scientific, 1:600) for 1h at RT ...