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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... in PBS containing 0.3 % Triton X-100 and incubated overnight at 4°C with a rabbit anti-Iba1 antibody (1:500, Wako) in PBS containing 1 % FBS (Gibco), 0.1 % BSA (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Blot sections were probed overnight at 4°C 1:5000 with the ATXN1 antibody 11750 (Servadio et al., 1995) or 1:2500 with H1 antibody (Invitrogen) diluted in 5% milk PBST ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Pathology 2020Quote: ... The aqueous phase was separated by centrifugation (21,000 g for 15 min at 4 °C) and incubated with isopropanol (1:1 ratio) and 25 μg of Glycoblue (ThermoFisher) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked in 5% normal goat serum + 2% BSA and 2% Triton-X for 1 h and incubated with the following primary antibodies overnight at 4°C: rabbit anti-GFP (1:1000, Invitrogen) and chicken anti-TH (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at 30V for 1 hour at 4°C in 1X NuPAGE™ buffer (REF NP0006-1, Life Technologies). After transfer ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...
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bioRxiv - Molecular Biology 2024Quote: ... mono-Methylated K35 overnight (1:100) at 4°C followed by 1:100 Alexa-Fluor 488 and Alexa-Fluor 555 (both Invitrogen) for 2 h at RT.
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bioRxiv - Cancer Biology 2022Quote: ... then incubated overnight at 4 °C with the following antibody: ERα (Dilution 1:1 µg/mL; Invitrogen, CAT: MA5-13191). IRDye secondary antibody (Dilution 1:20,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275; Phosphorylated-cofilin 1:500 Invitrogen #44-1072G and β-actin 1:3000 ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubating at 37°C in a 5% CO2 humidified incubator in DFGM supplemented with 5 ng mL−1 TGF-β1 (Thermo Fisher Scientific) and 100 μg mL−1 ascorbic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated overnight at 4°C with 1 mg/ml BSA and 4% sheep serum in PBST and one or more of the following: Alexa-fluor 488 Phalloidin 1:300 (Molecular Probes), anti-beta tubulin antibody 1:100 (E7 ...
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Cell Biology 2021Quote: ... hRobo1-FL-3xHA(C)/pcDNA3 or hRobo1ΔCC0-3-3xHA(C)/pcDNA3 using Lipofectamine2000 (Thermo Fisher Scientific) and incubated for 24 hours and then sub-cultured into 12-well culture dishes and allowed to bed down overnight ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were digested overnight at 37°C with 1-2 µL of the Lys-C (125-05061, Wako) and trypsin mixtures (Pierce, Thermo Fisher Scientific) (10 ng/μL each) ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Immunology 2021Quote: ... then 40 cycles at 95°C for 15s and 62°C for 1min on a Step One Plus Instrument (Applied Biosystems). All qPCR was performed in technical triplicate and the average of these replicates was used to generate one data point per mouse assessed ...
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bioRxiv - Molecular Biology 2022Quote: ... beads were washed five times with RIP buffer for 5 min at 4°C and RNA was extracted using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... to obtain images and then stored at 4°C in 5% acetic acid and 10% glycerol solution in a heat-sealable roll stock pouches (Fisher Scientific, #0181226E). Proteins in gels used for Western Blotting were then transferred to polyvinylidene difluoride membranes (Whatman) ...
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bioRxiv - Biochemistry 2021Quote: ... and then heated at 95 °C for 5 min before loading onto a pre-cast 4-12 % Bis-Tris polyacrylamide gel (Thermo Fisher Scientific). Samples were run at 200 V for 45 min before staining with Coomassie Blue.
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bioRxiv - Cancer Biology 2021Quote: ... Samples were heated at 95 °C for 5 min and run on a 4-12% Bis-Tris NuPage gel (Thermo Fisher, #NP0321). Samples were then transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... mouse splenic B-cells were added to coverslip chambers containing PLB coated with AF488-conjugated mono-biotinylated Fab’ goat-anti-mouse IgM+G and incubated at 37°C with 5% CO2 in the presence of 10 µg/ml FM 4-64FX (Thermo Fisher Scientific) for varying lengths of time ...
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bioRxiv - Physiology 2022Quote: 60 x 106 of mouse splenocytes were incubated for 15 min at 4°C in PBS containing 5% Normal Rat Serum (Thermo Scientific, 10710C) and anti-CD16/32 (BioLegend ...
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bioRxiv - Genomics 2021Quote: ... One OD600 absorbance unit (1.25 - 2.5 ml) of cells were collected and lysed at 95°C in 15 µL 4× NuPage LDS Sample Buffer (Thermo Fisher Scientific) with 5% β-mercaptoethanol for 10 minutes ...
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bioRxiv - Biophysics 2019Quote: ... heated at 100°C for 5 min and run on a 4 to 12% Bis-Tris SDS-polyacrylamide gel (Life Technologies, CA). Loading amounts of proteins were 1 μg for Coomassie staining and 0.5 μg – for Western Blot ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared at 16,000g for 15 min at 4 °C and supernatants were supplemented with 5 μl/ml of RNase A/T1 (Thermo Fisher Scientific) for 30 min at 4 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were washed twice with cold phosphate-buffered saline and lysed at 4 °C (on ice) in RIPA buffer (Wako) containing 5 mM EDTA and protease inhibitor cocktail (Thermo Fisher Scientific). The cell lysates were cleared by centrifugation ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were collected by centrifuging 5 min at 300 g and 4°C and resuspended in PBS containing 0.04% BSA (Thermo Fisher Scientific, #AM2616). The cell suspension was filtered to remove cell aggregates and particles with a diameter greater than 35 µm ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor488-labeled human transferrin was dissolved in PBS 5 at mg/ml and stored at 4°C (Thermo Fisher, ref. no. 13342). TexasRed-labelled neutral 3’000 and 40’000 Da dextran was dissolved in PBS at 10 mg/ml and stored at -20°C (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... 1% Triton X-100 and 5% goat serum and incubated overnight at 4°C and DNA was labeled using Hoechst (Thermo Fisher, H3570). Images were subsequently captured with confocal microscope ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by centrifugation at (21,000 g, 5 min, 4 °C) and endogenous metabolites were removed with Zeba Spin Desalting Columns (ThermoFisher, cat. no 89882). The lysate was then split into sample replicates for PISA (four replicates for each treatment) ...
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bioRxiv - Cell Biology 2024Quote: ... The crypts were centrifuged for 5 min at 300 × g at 4 °C and the pellets were gently resuspended in 800 μl TrypLE Express (Thermo Fisher Scientific) supplemented with 200 μl PBS followed by incubation in a water bath at 32 °C for 1.5 min ...
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bioRxiv - Cell Biology 2024Quote: ... the grids were blotted for 3.5 s at a humidity of 100% and 4°C and plunge-frozen in liquid ethane using a Vitrobot (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was then centrifuged at 300 × g for 5 min at 4 °C and cells resuspended in 100 µL of stain buffer (BD Biosciences, supplemented with 5 mM EDTA (Thermo Fisher Scientific)) ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were centrifuged at 400 g for 5 min at 4 °C with no brake and resuspended in staining buffer (DPBS (without Ca2+ and Mg2+) (Gibco, Life Technologies) containing 0.1 % low endotoxin BSA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were boiled at 95°C for 5 min and 10 µl of each sample was separated by 4-12% Bis-Tris SDS-PAGE (Thermo Scientific NW04122BOX) in 1×MES buffer (Thermo Scientific B0002 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Genomics 2023Quote: Adults were relaxed by incubating for 3–16 hours at 4 °C in about 400 mL ASW with approximately 5 g of 99% L-menthol crystals (Thermo Scientific A10474) (3 adults per 400 mL volume) ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were heated at 99 °C for 5 min and separated using Novex Precast 4–20% gels (Invitrogen, Waltham, MA, #WXP42020BOX) in SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were washed five times with RIP buffer for 5 min at 4°C and RNA was extracted using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Reduced samples (by addition of 2-mercaptoethanol) were heated at 95°C for 5 minutes then loaded onto a 4-12% tris-glycine gradient gel (Thermo Fisher, XP04122BOX) and ran at 125 volts for 1-1.5 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Four μl of sample with an OD260 of 7.5 were applied to the grids and vitrified at 4 °C and 100 % humidity using a Vitrobot Mk IV (Thermo Fisher Scientific) (blot 6s ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then boiled at 95°C for 5 min and electrophoresed using a NuPAGE Bis-Tris Gel (4-12%) with NuPAGE SDS Running Buffer (Thermo Fisher Scientific) for 2 hr at 130 V ...
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bioRxiv - Genetics 2024Quote: ... Samples were denatured at 99°C for 5 minutes and separated on a 4-20% tris-glycine protein gel (Thermo Fisher Scientific) at 150 V for 75 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... DAPI ((4’,6-diamidino-2 phenylindole, Invitrogen) was added and samples were covered with coverslips ...
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bioRxiv - Immunology 2020Quote: ... and Fluo-4 AM (Invitrogen, 2 μM) probes for 30 min before fixation with 1% PFA for 4 hours ...