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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Probe 2 (mutation; 5’ FAM – CTC CCG CCT GAT T 3’) and Platinum Quantitative PCR SuperMix-UDG w/ROX (Life Technologies). The products were amplified and analysed on an ABI StepOne PCR machine.
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bioRxiv - Cancer Biology 2024Quote: ... Recipient cells were incubated with either PKH67 dye-labelled or fluorescent-labelled sEVs for 2-3 days in media supplemented with 5% exosome-depleted FBS (Gibco, USA). Cells were imaged with time-lapse microscopy or washed with PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... 5% mercapto-2-ethanol and 1X Halt Protease Inhibitor Cocktail (Thermo Scientific; 1:200). Proteins were separated using SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 1xPBS supplemented with 2 % fetal bovine serum (FBS, Hybclone) and 1 μg/mL 7-AAD (Life Technologies), filtered through a 40 μm strainer ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was centrifuged at 1000 g for 3 min at 4 °C to remove debris and then the supernatant was incubated with anti-HA magnetic beads (Thermo Fisher Scientific, cat. # 88837) for 6 minutes at 20 °C ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Cell Biology 2020Quote: ... Gonads were incubated overnight at 4°C with 1 U/ml Texas Red-X Phalloidin (ThermoFisher Scientific) in staining solution (1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated overnight at 4°C with antibodies directed against CD44 (Thermo Scientific, MA4400; 1:200) and CD56 (Leica Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C and anti-mouse-HRP-coupled secondary antibody (1:3000, Life Technologies) was incubated for 1.5 h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated overnight at 4°C with monoclonal primary anti-GFP antibody from rabbit (1:2,000) (Invitrogen), and with secondary anti-rabbit antibody (1:30,000 ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed overnight at 4°C with monoclonal α-FLAG (#31430, ThermoFisher Scientific; 1:10,000) antibody diluted in 1×TBS-T buffer containing 3% bovine serum albumin ...
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bioRxiv - Physiology 2021Quote: ... The sections were then incubated overnight at 4°C with polyclonal anti-laminin (1:1000; ThermoFisher Scientific) primary antibody diluted in blocking solution ...
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bioRxiv - Genetics 2022Quote: ... at room temperature then incubated overnight at 4°C with mouse anti-v5 (1:1000, Invitrogen, RRID:AB_2556564), and rat anti-tubulin alpha (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... and for 48 hours at 4°C followed by 15 minutes of DAPI staining (1:5000; ThermoFisher). Retina whole mounts were also incubated with rabbit anti-Alexa-fluor 488 conjugated GFP for 4 days at 4°C followed by 15 minutes of DAPI staining ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4 °C followed by goat anti-rabbit Alexa-488 (1:200, Invitrogen, Waltham, MA, USA) for 2 hours at room temperature and DAPI (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with primary antibodies overnight at 4 °C (1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Systems Biology 2022Quote: ... The samples were centrifuged at 100,000×g for 1 h at 4°C (S55A2 rotor, Thermo fisher). The pellets were resuspended in SDS sample buffer and subjected to western blot analysis.
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bioRxiv - Cell Biology 2021Quote: ... primary antibodies overnight at 4°C followed by Alexa Fluor-647 secondary antibodies (ThermoFisher Scientific, 1:500) for 2hrs at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... PVDF membrane was incubated at 4 °C with 1:1000 ratio of PIKFYVE antibody (Invitrogen, PA5-13977) or 1:1000 ratio of α/β-Tubulin antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with primary antibodies overnight at 4 °C: pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:1000 dilution), STAT1 (Santa Cruz Biotechnology SC-417 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were probed with an SLC35A2 polyclonal antibody overnight at 4°C (1:2000, Invitrogen, PA5-77178), followed by incubation with goat anti-rabbit HRP-linked secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained using primary antibodies overnight at 4°C: tubulin (Invitrogen MA1-80017, 1:1000), aSyn (BD Transduction Laboratories 610786 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated at 4°C for 30 min with antibodies against CD45 (Invitrogen, #MCD4528, 1/25), CD31 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL (10μM) reverse primer (ldh4_R, 5’-AATCACAGCAGCCCCTTG-3’) and 1 μL (1 U/μL) Platinum Taq DNA polymerase (Invitrogen, 10966-018) in a 50 μL total reaction volume ...
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bioRxiv - Microbiology 2024Quote: ... Tenfold dilutions were used to infect confluent Vero E6 cells in a 96-well plate in MEM (5% FBS, 1% penicillin-streptomycin, 1% kanamycin, 3% amphotericin B (Gibco)) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml IL-4 (Life Technologies), and evaluated on day 6 of culture ...
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bioRxiv - Microbiology 2022Quote: ... 4 µL linear acrylamide (5 mg/mL, ThermoFisher), and 1 mL of ice-cold 96% ethanol followed by overnight incubation at −20°C.
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bioRxiv - Systems Biology 2023Quote: ... 4 μL 5× phusion HF buffer (Thermo Scientific), 4 μL 5 M Betaine (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Raji cells were then labeled with 10 μM CMAC (7-amino-4-chloromethylcoumarin, Molecular Probes), pulsed with 1 μg/ml SEE (Staphylococcus enterotoxin E ...
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bioRxiv - Bioengineering 2020Quote: HTM cells ± DEX at 7 d were fixed with 4% paraformaldehyde (PFA; Thermo Fisher Scientific) at room temperature for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μM Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) dye (Life Technologies, Carlsbad, CA) was added to 1 ml of mid-log cells 1 hour prior to imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Biochemistry 2020Quote: ... one µg of small RNAs was mixed with 2 µg of HPDP-Biotin (ThermoFisher Scientific, 1 mg/ml in DMSO). The reaction was incubated for three hours at room temperature protected from light and mixed every 15 minutes ...
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bioRxiv - Genetics 2019Quote: ... BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Bioengineering 2022Quote: Constructs were incubated for 1 hour at 37°C with 3 mM Fluo3 AM calcium indicator (ThermoFisher Scientific) in differentiation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... the cell pellets were incubated for an hour in 2 uM 2’,7’-dichlorodihydrogen-fluorescein diacetate (H2DCF-DA, Invitrogen) in phenol red free media (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Target cell killing was measured using CellEvent™ Caspase-3/7 Green Detection Reagent (Life Technologies) and analyzed by flow cytometry.
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bioRxiv - Neuroscience 2019Quote: Dead and apoptotic cells were detected using CellEvent Caspase-3/7 Kit (#C10423, Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase activity was assessed using CellEvent(tm) Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen) following manufactures protocol ...
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bioRxiv - Genomics 2021Quote: ... Laser Capture Microdissection (LCM; N=3; 7 µM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522), multiplex or regular immunohistochemistry (N≥3 4 µM glass slide ...
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bioRxiv - Developmental Biology 2024Quote: ... follicles were incubated with 500 nM CellEvent™ Caspase-3/7 Green Detection Reagent (C10427, Invitrogen). Oocytes were stained with 150 nM Sir-Tubulin (CY-SC002 ...
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bioRxiv - Cell Biology 2020Quote: ... and Y14 siRNA (5’-GGGUAUACUCUAGUUGAAUUUCAUAUUCAACUAGAG-3’) with Lipo2000 (Invitrogen) in Optimem (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5’-UGGUUUACAUGUCGACUAA-3’ KIF18A (Silencer Select s37882 – Ambion)8 5’-UCUCGAUUCUGGAACAAGCAG-3’ RAD51 (Silencer Select s11735 – Ambion) 5’-UGAUUAGUGAUUACCACUGCT-3’ RRM1 (On-Target plus SMARTpool – Dharmacon)
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bioRxiv - Genetics 2024Quote: ... 5′-UUAAUUUACGCGGUUUUUAUU-3′) using the RNAiMAX transfection reagent (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were fixed overnight at 4° C temperature in 4% paraformaldehyde in PBS buffer (Invitrogen) and incubated in 70% ethanol solution after three times’ wash with PBS buffer for paraffin section ...
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bioRxiv - Microbiology 2020Quote: ... incubated at 37°C (Cytomat 2, Thermo Scientific) with continuous shaking and OD595nm was measured every 30 min for 16 h in a Filtermax F5 multimode plate reader (Molecular Devices) ...