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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... coupled to an Acclaim PepMap 100 column (2 cm x 75 μm, 3 μm particle size, Thermo Fisher Scientific) coupled in line with an EASY-Spray PepMap RSLC C18 reversed-phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2023Quote: ... Muropeptides were separated using a C18 analytical column (Accucore TM C18, 2.6µm particles, 100 × 2.1 mm; Thermo Fisher Scientific), column temperature at 50°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were injected onto an Easy Spray PepMap C18 column (75μm id × 25 cm, 2μm particle size) (Thermo Scientific) and separated over a 2 hr method ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed from non-bound particles by centrifugation and resuspended in 400 µL of colorless FluoroBrite DMEM medium (Gibco, USA). Next ...
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bioRxiv - Physiology 2023Quote: ... The analysis was performed on a Accucore Vanquish C18+ column (100 x 2.1 mm, 1.5µm particle size; ThermoFisher Cat. #20073385). The following mobile phases were used ...
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bioRxiv - Biophysics 2023Quote: ... Negatively stained virus particles were observed on a 30 kV scanning electron microscope (Thermo Fisher Scientific, Quanta FEG 650) in scanning transmission electron microscopy (STEM ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were then separated (EASY-Spray; PepMap RSLC C18, 75 μm × 50 cm, 2 μm particles, Thermo Fisher Scientific) at a flow rate of 250 ηL min-1 employing a two-step gradient ...
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bioRxiv - Bioengineering 2023Quote: ... HILIC separation was employed with a Waters ACQUITY UPLC BEH amide column (2.1 ×150 mm, 1.7 μm particle size) on a Vanquish (ThermoFisher Scientific) chromatograph ...
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bioRxiv - Cancer Biology 2023Quote: ... reverse-phase EASY-Spray column ES802A (250 mm long) packed with 2 μm C18/silica particles (Thermo Fisher Scientific). Column equilibration was performed with buffer A (H2O 96 % ...
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bioRxiv - Bioengineering 2024Quote: ... and treated with purified viral particles resuspended in 0.8 mL DMEM containing 8 µg/mL polybrene (Thermo Fisher Scientific). The cells were spinfected by centrifuging the six-well plates at 1050 x g for 90 min at 30 °C and the returned to the 37 °C incubator for another 48 h ...
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bioRxiv - Systems Biology 2023Quote: ... Separation of lipid compounds in both positive and negative ion mode was achieved by reverse-phase liquid chromatography on an Accucore C30 column (250 x 2.1 mm, 2.6 µm particle size, Thermo Scientific) at 35°C column temperature with a 200 µL/min flow rate ...
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bioRxiv - Molecular Biology 2024Quote: ... Digested nucleosides were separated using a Hypersil GOLD aQ C18 column (100-mm length × 2.1-mm inner diameter, pore size 175 Å, particle size 1.9 µm; (ThermoFisher) at a flow rate of 0.4 mL/min using 0.1% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μm particle size) using a gradient of increasing acetonitrile delivered by a Vanquish Neo UHPLC-System (Thermo Scientific) operating at a flow rate of 500nL/min ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were injected onto a 300μm x 5mm PepMap Neo Trap Cartridge peptide trap column (C18, 5μm particle size, 100Å pore size, ThermoFisher Scientific) and separated on an EASY-Spray 75μm x 50cm PepMap HPLC column (C18 ...
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bioRxiv - Biochemistry 2024Quote: ... and separated on an EASY-Spray 75μm x 50cm PepMap HPLC column (C18, 2μm particle size, 100Å pore size, ThermoFisher Scientific) at a flow rate of 300 nL/min at 40°C using a multistep gradient from 2-30% mobile phase B (80% MeCN in 0.1% formic acid ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were injected onto a 300μm x 5mm PepMap Neo Trap Cartridge peptide trap column (C18, 5μm particle size, 100Å pore size, ThermoFisher Scientific) and separated on an EASY-Spray 75μm x 50cm PepMap HPLC column (C18 ...
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bioRxiv - Biochemistry 2024Quote: ... and separated on an EASY-Spray 75μm x 50cm PepMap HPLC column (C18, 2μm particle size, 100Å pore size, ThermoFisher Scientific) at a flow rate of 250 nL/min at 40°C using a multistep gradient from 2-30% mobile phase B (80% MeCN in 0.1% formic acid ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with a trapping column (3 µm C18 particle, 2 cm length, 75 µm ID, Acclaim PepMap, Thermo Scientific) and a 50 cm long separation column (2 µm C18 particle ...
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bioRxiv - Microbiology 2024Quote: ... VSV particles were subsequently PEG-precipitated form the cleared medium and resuspended in FluoroBirte DEMEM medium (Thermo Fisher Scientific) containing 5% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2021Quote: ... 5% human serum and 5% AlbuMAX-II (Gibco)) was replaced daily until reaching maturity at day 14-post induction ...
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bioRxiv - Immunology 2023Quote: ... 2mg of 5-ethynyl uridine (5-EU, Thermofisher) was injected intraperitoneally at D12 following SRBC immunisation and similar preparation and labelling steps described for the ex vivo 5-EU assay were followed ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T were transfected with lentiviral and packaging vectors (psPAX2 and p-CMV-VSV-G) using Lipofectamine 2000 ® reagent and OPTI-MEM media (ThermoFisher Scientific, Massachusetts, EEUU) for 4h following the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... OFTu cells were plated in a 24-well plate and 24h later transfected with 500 ng of the pBS185-CMV-Cre plasmid using Lipofectamine 3000 (Invitrogen, catalog num: L3000-075) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The NFE2L2 ORF was cloned into the pLenti-CMV-Puro vector using a Gateway LR Clonase II kit (Cat# 11791-020, Thermo Fisher Scientfic, Waltham, MA). The cytosolic GPX4 (cGPX4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The MCL1 ORF was cloned into the pLenti CMV vector using a Gateway LR Clonase II kit (Cat# 11791-020, Thermo Fisher Scientfic, Waltham, MA). The resulting plasmid is missing a stop codon ...
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bioRxiv - Molecular Biology 2020Quote: ... driven by the human cytomegalovirus (CMV) immediate early enhancer and promoter was assembled using the GeneArt CRISPR Nuclease Vector system (Thermo Fisher Scientific, Waltham, MA), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Immunology 2024Quote: ... Luciferase-labeled cell lines were generated through infection with pTomo-CMV-luciferase-IRES-puro lentivirus (MOI≈10) and subsequently selected with puromycin (Life Technologies, 1 µg/ml) for 2 weeks ...
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bioRxiv - Microbiology 2024Quote: ... All redesigned RSV-F and hMPV-F constructs were subcloned into a CMV/R vector and transiently expressed in ExpiCHO cells (Thermo Fisher, catalog no. A29133) as previously reported (69 ...
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bioRxiv - Plant Biology 2019Quote: ... The database search was limited to “Only Columbia-0 Wild Type from Affymetrix Arabidopsis ATH1 genome array” ...
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bioRxiv - Synthetic Biology 2021Quote: ... The vector was digested with the Type IIS restriction enzyme AarI (Thermo Fisher) for 4 hours at 37°C and treated with Antarctic phosphatase (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... DQ™-collagen type I (Life Technologies/Thermo Fisher Scientific, Carlsbad, CA, USA) was mixed with collagen gels at a final concentration of 25 μg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... Wild-type zygotes were injected with Alexa-488 dextran (Thermo Fisher Scientific, D22910) and used for donor embryos ...
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bioRxiv - Cell Biology 2020Quote: ... Probes used in this study are ViewRNA Type 6 probes from Thermo Fisher: CDC42EP1 (VA6-3170107-VC) ...
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bioRxiv - Developmental Biology 2021Quote: ... and then exposed to Type IV Collagenase (2 mg/mL; Thermo Fisher Scientific) at 35°C for 20 minutes with periodic shaking ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were incubated with 1 mg/ml of collagenase Type I (Invitrogen) at 37°C for 15-30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and digestion with a solution containing type II collagenase (0.2% w/v; Gibco) and trypsin (0.25% w/v ...
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bioRxiv - Genomics 2020Quote: ... and bacterial DapB (negative control, Type 1 Probe, VF1-11712, Thermo Fisher Scientific). LECs were additionally co-stained with mouse anti-human CD31 antibody (clone JC70A ...
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bioRxiv - Physiology 2020Quote: Heart were dissociated using 2 mg/mL type IV collagenase (Gibco™, ThermoFisher) for 1 hour at 37°C and the resulting dissociated cells were filtrated on a 30 µm strainer ...
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bioRxiv - Physiology 2020Quote: Heart were dissociated using 2 mg/mL type IV collagenase (Gibco™, ThermoFisher) for 1 hour at 37°C and the resulting dissociated cells were filtrated on a 30 µm strainer ...
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bioRxiv - Genetics 2019Quote: Total RNA was extracted from 35 wild-type larvae using TRIzol (Invitrogen, USA) in successive days from 5dpf to 8dpf ...
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bioRxiv - Developmental Biology 2020Quote: ... Collagen Type I (DSHB at the University lowa, SP1-D8 and Invitrogen, PA529569), EOMES (R&D Systems ...
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bioRxiv - Genetics 2021Quote: Wild-type human FAN1 gene was cloned in a derivative of pFASTBAC1 (Invitrogen) with GST- ...
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bioRxiv - Bioengineering 2020Quote: ... and 50 µL cell type-specific medium (RPMI1640 – mouse; complete DMEM/F12 (Gibco) – human) ...
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bioRxiv - Neuroscience 2020Quote: ... and 250 U/mL DNase I type IV in neurobasal medium (Life Technologies)] ...
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bioRxiv - Cancer Biology 2020Quote: ... orthotopic pancreatic tumors were harvested and enzymatically digested with Type IV Collagenase (Gibco) and Deoxyribonuclease I (Alfa Aesar) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellets were incubated with 0.2% Collagenase type 2 (Thermo Fisher Scientific, 17101015) in high glucose DMEM for 90 mins in a 37°C shaking water bath ...
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bioRxiv - Biophysics 2020Quote: ... low-confluency (∼30%) wild-type HEK293T cells were transiently transfected (Liopfectamine 3000, ThermoFisher) with 1 µg of the relevant pX458 plasmid and 2 µg of ssDNA repair template ...
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bioRxiv - Cell Biology 2022Quote: ... The gel was coated with type I collagen (25 μg/ml) (Invitrogen; A1048301) using Sulfo-SANPAH based conjugation and kept at 4°C overnight(41).