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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... TGF-β and RANTES using custom ProcartaPlex assay kits (ThermoFisher Scientific). The analyte concentrations were determined using a Luminex 100/200 Flexmap3D instrument coupled with the Luminex XPONENT software.
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bioRxiv - Microbiology 2022Quote: ... and 1:100 dilution of β-actin (rabbit polyclonal antibody, Invitrogen). A 1:3000 dilution of goat-anti-mouse IRDye 800CW ...
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bioRxiv - Molecular Biology 2023Quote: ... were induced with 1mM IPTG (Isopropyl β-d-1-thiogalactopyranoside, ThermoFisher) at 25°C for approximately 16 hours ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% β-mercaptoethanol (Thermo Fisher Scientific, 21-985-023). Full-length cDNA and sequencing libraries were prepared from 1 ng RNA using the Smart-Seq2 protocol as previously described (Picelli et al. ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin and 1/1000 β-mercapto-ethanol (Life Technologies) in 96-well flat-bottom plates 24 hour prior to co-culture ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin and 1/1000 β-mercapto-ethanol (Life Technologies) in 96-well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each ...
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bioRxiv - Biochemistry 2023Quote: ... N-dodecyl β-D- maltoside (DDM,10% stock, Thermo Fisher Scientific) was added to the sample to a final concentration of 1% and the mixture was incubated on ice for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... β-lactamase activity was detected using the ToxBLAzer Dual Screen (Invitrogen), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... β-lactamase activity was detected using the ToxBLAzer Dual Screen (Invitrogen), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... UK) and an antibody to β-Actin was obtained from ThermoFisher. Antibodies to hemagglutinin (HA) ...
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bioRxiv - Immunology 2023Quote: ... Cells were co-stained for β-tubulin (480011, Thermo Fisher Scientific) with signal detected by Alexa Fluor 555 (AF555 ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-β-actin (cat# AM4302 or cat# MA1-744 Thermo Fisher), RNF8 (cat# sc-271462 ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit anti-β-gal (1:200, Thermo Fisher Scientific #A111-32). Alexa-conjugated goat-anti-mouse and anti-rabbit antibodies were used as secondary antibodies (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... 2.5 mM HEPES and 50 µM β-mercaptoethanol (all from Gibco) and divided into unstimulated and stimulated wells ...
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bioRxiv - Microbiology 2023Quote: ... We used an anti-β actin antibody from ThermoFisher (MA1-140) at 1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and β-Actin monoclonal HRP (Thermo Fisher Scientific MAF-15739-HRP).
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2.75 mM β-mercaptoethanol (Thermo Fisher Scientific, 21985-023) and denatured for 5 min at 90° C ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin were cloned into human expression vectors driven by the CMV promoter (Table 3) and co-transfected into FreeStyle 293-F cells (Thermo Fisher Scientific). The empty 5Myc plasmid (CS2P ...
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bioRxiv - Immunology 2021Quote: ... the mutated NLRP3 and WT NLRP3 were transferred into CS-IV TRE CMV KT (kindly provided by Dr. H. Miyoshi, RDB12876, RIKEN BRC, Tsukuba, Japan) with LR clonase (Thermo Fisher Scientific). To develop NLRP3-mNeonGreen or ASC-BFP-expressing lentiviral vector ...
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bioRxiv - Microbiology 2019Quote: ... the molecular clone (pBR322(CMV)-JEV-RP9) was transfected into C6/36 cells using Lipofectamine 2000 (Life Technologies; catalog no. 11668-019). Once a cytopathic effect was visible ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were infected with lentivirus carrying the pLenti CMV rtTA3 Blast (w756-1) plasmid and selected with blasticidin (Cat# A1113902, Thermo Fisher Scientific). Genes of interest were cloned into the pLenti CMVTRE3G eGFP Neo (w821-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... MCF10A cells were co-transfected with 1000 ng of pHrD-IRES-Luc plasmid 16 and 0.1 ng of a CMV-Renilla constitutive internal control plasmid using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids containing EGFP expression cassette under the control of AdV-C5 E1A or cytomegalovirus (CMV) promoter and enhancer regions were transfected at an amount of 100 ng / well using Lipofectamine 2000 transfection method (ThermoFisher Scientific, 11668019). Cells were fixed 48 h post transfection ...
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bioRxiv - Neuroscience 2022Quote: ... L19N-RhoA (dominant negative inactive)], as well as, the reporter vector (GST::PEGB, MYC::PRK5, Flag::CMV using lipofectamine 2000 reagent (1mg/ml) (Invitrogen, 11668-019). Opti-MEM (Minimal Essential Medium) ...
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bioRxiv - Genetics 2022Quote: ... 500ng of pRK5-CMV-Myc-Pum1 or pRK5-CMV-Myc-Empty plasmids were transfected in antibiotic-free DMEM (GenDepot, #CM002-320) using Lipofectamine LTX with Plus Reagent (Thermo Fisher, #15338030) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... CRE-nls-GFP ORF was amplified from addgene plasmid #49056 and inserted into the adenoviral expression vector pAd/CMV/V5-DESTTM Gateway Vector plasmid (Thermo Fisher Scientific). After production and amplification of the adenovirus ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: H1299 cells were transfected with the pCDNA3-mCherry-SEpHluorin (Koivusalo et al., 2010) (mCh-pHl) or pLenti-CMV-Blast-PIP-FUCCI using Lipofectamine2000 (Life Technologies, 11668019) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... HER2 with a C-terminal tail truncation (λ−1030–1255) followed by maltose binding protein (MBP) and twin-strep tags was cloned into pFastBac1 with a CMV promoter (Thermo Fisher Scientific). Point mutations were introduced in the HER2 kinase domain ...
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bioRxiv - Cell Biology 2023Quote: ... The LR reaction of the Gateway system was performed to recombine pENTR3c-Dusp13-IRES-EGFP into pAd/CMV/V5-DEST (Thermo Fisher Scientific). In total ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Cell Biology 2020Quote: ... a 0.075 × 150 mm-EASY-Spray column (3-μm particle diameter, 100 Å pore size, Thermo Fisher Scientific) with mobile phases of 0.1% formic acid and 0.1% formic acid/80% acetonitrile was used ...
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bioRxiv - Molecular Biology 2021Quote: ... Assessment of Ion Sphere Particle quality was undertaken using the Ion Sphere Quality Control Kit (4468656, ThermoFisher Scientific). Triplicate libraries were pooled together to give treated and untreated pools and sequenced initially using Ion 314 chips V2 (4482261 ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were separated using an analytical C18 Acclaim PepMap column (75µm x 250 mm, 2µm particles; Thermo Scientific) at a flow rate of 300 µl/min using a 58-min gradient ...
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bioRxiv - Biochemistry 2021Quote: Single-particle cryo-EM data was collected on a Titan Krios 300 kV cryo-electron microscope (ThermoFisher Scientific) in the Pacific Northwest CryoEM Center (PNCC ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were generated with pCMV-VSVG and psPAX2 packaging plasmids according to the manufacturer (Thermo Fisher Scientific). Viruscontaining supernatant was collected 48 hours post transfection and used at a multiplicity of infection of 5 ...
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bioRxiv - Cancer Biology 2021Quote: ... along with 0.5μg pCMV-VSV-G-RSV-Rev and 1μg pCAG-HIVgp for viral particle preparation using Lipofectamine 2000 (Invitrogen)-mediated transfection ...
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bioRxiv - Cell Biology 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 µm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Microbiology 2020Quote: ... A Hypersil Gold C18 column (1.9 μm particle size, 50 × 2.1 mm internal diameter; Thermo Fisher Scientific, Australia) was used for elution ...
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bioRxiv - Microbiology 2022Quote: ... place tubes containing streptavidin MagneSphere paramagnetic particles on a magnetic stand (DynaMag™-2 Magnet, Life Technologies 12321D) for 30s – 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... equipped with an Acclaim Pepmap C18 trap column (2 cm * 75 µm, particle size: 3 µm; Thermo Fisher) and a C18 analytical column (50 cm * 75 µm ...
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bioRxiv - Genomics 2022Quote: ... the dialyzed cell particles were mixed with 300 μl of Pierce™ NHS-Activated Magnetic Beads (Thermo Fisher), which was pre-washed with 1 mM hydrochloride solution ...
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bioRxiv - Immunology 2022Quote: ... RAW 264.7 cells were transduced with the lentiviral particles and then selected with 4 μg/mL puromycin (Gibco). After 5 days of selection ...
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bioRxiv - Biophysics 2019Quote: ... coli OP50 mixed with 1 μm-diameter paramagnetic particles (Dynabeads MyOne Streptavidin C1, Invitrogen, Thermo Fisher Scientific, USA), Group 1 ...
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bioRxiv - Biophysics 2019Quote: ... coli OP50 mixed with 1 μm-diameter paramagnetic particles (Dynabeads MyOne Streptavidin C1, Invitrogen, Thermo Fisher Scientific, USA), Group 1 ...
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bioRxiv - Genomics 2020Quote: ... HEK293FT cells for production of viral particles were maintained in DMEM supplemented with 10% FBS (Thermo Fisher Scientific), 1% Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HCMV DNA inside cell-free particles was purified using the PureLink Viral RNA/DNA mini kit (Thermo Scientific). A region within UL83 conserved among ME ...
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bioRxiv - Microbiology 2021Quote: ... The enriched template-positive Ion sphere particles were loaded onto an Ion 318 chip v2 (Thermo Fisher Scientific) and sequenced on an Ion PGM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Single chitin particles (85.0±24.0 µm in diameter) were transferred to sterile 96-well plates (Thermo Fisher, #AB0600L), with one chitin particle per well ...
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bioRxiv - Cell Biology 2019Quote: HEK293doxIRE1β cells were established by subsequent transduction of low passage HEK293 cells (obtained from ECACC) with lentiviral particles encoding the pLenti3.3 doxycycline-controlled regulator and pLenti6.3/V5/ERN2-FLAG (Invitrogen). ERN2-FLAG coding sequence was introduced by Gateway cloning ...