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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: 100μl of reovirus particles (from 1013 particles/ml stock) were mixed with 0.4μl of Alexa488 or Alexa647 NHS Ester (8mM starting concentration) (Thermo Fisher Scientific) for 1h at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and separated on a 150 mm PepMap C18 column (150 mm length, 75 μm i.d., 2 μm particle size, Thermo Scientific) heated to 65° C using a 30 minute gradient from 2 to 35% mobile phase B (95% acetonitrile ...
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bioRxiv - Cell Biology 2019Quote: ... Actin filaments and microtubules were stained with RFP fluorescent dyes using BacMam technology (CellLight®, Thermofisher, 7.5^105 particles/mL). All studies were performed between 3-4 days post-transduction or transfection.
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bioRxiv - Cancer Biology 2021Quote: ... Two microliters from each fraction were resolved in 2D on a nanocapillary reverse phase column (Acclaim PepMap C18, 2 micron particle size, 75 μm diameter × 50 cm length, ThermoFisher) using a 0.1% formic/acetonitrile gradient at 300 nL/min (2–22% acetonitrile in 150 min ...
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bioRxiv - Microbiology 2021Quote: ... Substrate and reaction products were separated by HPLC on an ODS-Hypersil 3 µm particle-size C18 column (250 × 4.6 mm; Thermo Scientific). Elutions were performed with 50 mM sodium phosphate buffer ...
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bioRxiv - Immunology 2021Quote: LC separations were performed with an EASY-nLC 1200 UHPLC system by using an EASY-Spray column ES802A (250 mm long, 75 μm i.d., 2 μm C18/silica particles, ThermoFisher Scientific) operated at 250 nL/min ...
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bioRxiv - Microbiology 2022Quote: ... Single-particle cryo-EM data were collected as described previously (26, 31, 37) on a Titan Krios G3 microscope (ThermoFisher) operating at 300 kV fitted with a Quantum energy filter (slit width 20 eV ...
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bioRxiv - Microbiology 2022Quote: ... connected to a PepMap C-18 trap-column (0.075 x 50 mm, 3 m particle size, 100 pore size; Thermo Fisher) and an in-house-packed C18 column μ (column material ...
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bioRxiv - Microbiology 2022Quote: ... was used to produce viral particles from infectious clones and were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (1X) + GlutaMax media (#61965-026, ThermoFisher Scientific) complemented with heat-inactivated Fetal Calf Serum (#A3840002 ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the trap column was switched in-line with an Acclaim PepMap nanoHPLC C18 analytical column (2.0 µm particle size, 75 µm ID x 500 mm, catalog number 164942, Thermo Scientific). The column temperature was maintained at 50 °C ...
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bioRxiv - Microbiology 2022Quote: ... Lysed viral-like particles were then treated for 30 min at 55 °C with Proteinase K (20 mg/mL, Lot No. 1112907, Invitrogen) to digest the viral protein capsid ...
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bioRxiv - Cell Biology 2022Quote: ... and control cell lines were obtained by transduction with retroviral particles generated in Hek293T cells and sustained selection with puromycin 2 μg ml-1 (A1113803, ThermoFisher). Specifically ...
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bioRxiv - Cancer Biology 2022Quote: ... (C18 PepMap100, 75 µm ID x 25 cm length, 2 µm particle size, 100 Å pore size, Thermo Fisher Scientific). The 120-minute gradient was programmed as ...
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bioRxiv - Neuroscience 2023Quote: ... particle size = 3 μm, C18, L = 2 cm; analytical column: particle size = 2 μm, C18, L = 50 cm; PepMap, Dionex/Thermofisher). Peptides eluting from the column were ionized using an Orbitrap Elite (Thermofisher).
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bioRxiv - Microbiology 2023Quote: DNA from microscopy-quantified blood stage parasites [33] was extracted from 5µL blood using a semi-automatic Kingfisher Flex Magnetic Particle Processor and MagMAXTM-96 DNA Multi-Sample Kit (ThermoFisher Scientific) as per [34] ...
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Visualizing translating dynamics in situ at high spatial and temporal resolution in eukaryotic cellsbioRxiv - Biophysics 2023Quote: Cryo-lamellae were collected by applying a single particles data collection method using FEI Titan Krios G3 (Thermo Fisher Scientific) equipped with a Gatan GIF K2 camera (Gatan ...
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bioRxiv - Cancer Biology 2023Quote: ... Lipids were separated on a C8 column (Accucore C8 column, 2.6 µm particle size, 50 × 2.1 mm, Thermo Fisher Scientific) mounted on an Ulitmate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Peptides were separated on a 40 cm x 75 µm inner diameter PepMap column packed with 1.9 µm C18 particles (Thermo Fisher) using Dionex nanoUHPLC ...
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bioRxiv - Molecular Biology 2023Quote: ... High titre (>3 × 108 viral particles per ml) recombinant baculovirus was obtained using the Bac-to-Bac expression system (Invitrogen) in Sf9 (Spodoptera frugiperda ...
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bioRxiv - Molecular Biology 2023Quote: ... then bound to an EasySpray C18 column (2 µm particle size, 75 µm ID x 50 cm length, ThermoFisher Scientific) and eluted over the following 60 min gradient ...
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bioRxiv - Microbiology 2023Quote: ... Milled particles were sieved through a series of different pore sizes (500 μm, 850 μm, 1250 μm) (Fisher Scientific, UK) and particles larger than 1250 μm were re-processed as above until they passed through the 1250 μm sieve.
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bioRxiv - Cancer Biology 2023Quote: Metabolites were separated using a XSelect HSS C18 column (2.1 mm x 150 mm, 3.5 μm particle size) (Waters, Milford, MA) in an UltiMate 3000 quaternary UHPLC (Thermo Scientific) equipped with a refrigerated autosampler (5°C ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse Phase separation was employed with an Accucore™ C30 column (2.1×150 mm, 2.6 μm particle size) on a Vanquish (ThermoFisher Scientific) chromatograph ...
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bioRxiv - Cell Biology 2023Quote: ... packed with Halo C18 reversed-phase resin (2.7 µm particle size, 90 nm pore size, MichromBioresources.) was used with EASY-nLC 1200 system (Thermo Fisher). The nanospray ESI was fitted onto the Thermo Q-Exactive plus mass spectrometer (Thermo Electron ...
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bioRxiv - Cell Biology 2022Quote: ... Three separate repeats of pQ-EV and pA-EV isolations were pooled upon normalization for particles/mL for ever sample and used for hydrophilic interaction liquid chromatography-mass spectrometry analysis (UltiMate 3000 RSLC, ThermoFisher, with a 150 x 4.6mm ZIC-pHILIC column running at 300µL/min-1 and Orbitrap Exactive) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells (a gift from H. Choe) used for generation of lentiviral particles were maintained in DMEM (Gibco #11995-065) supplemented with 10% fetal bovine serum (Sigma F0926 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV particles were captured by affinity chromatography using POROS™ CaptureSelect™ resins (AAV9 and AAVX, Thermo Fisher Scientific, MA). AAV suspension was concentrated using centrifugal devices (Amicon® Ultra-15 100 kDa centrifugal filter devices ...
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bioRxiv - Biochemistry 2023Quote: ... the trap column was switched in-line with an Acclaim PepMap nanoHPLC C18 analytical column (2.0 µm particle size, 75µm IDx500mm, # 164942, Thermo Fisher Scientific). The column temperature was maintained at 50 °C ...
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bioRxiv - Microbiology 2023Quote: ... 2 μL sample was loaded onto an Hypersil GOLD column (particle size 1.9 μm, 100 × 2.1 mm, Thermo Fisher Scientific) connected to an Hypersil GOLD guard column (particle size 3.0 μm ...
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were reprogrammed into iPS cells using transient overexpression of pluripotency-associated factors (OCT4, KLF4, and SOX2) via non-integrating Sendai virus particles (Cytotune 2.0 kit, Life Technologies). At least five clones per TSC2+/+ and TSC2+/− donor were established into iPS cell lines ...
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bioRxiv - Molecular Biology 2024Quote: Retroviral particles were produced by transfecting pLPC plasmids into low passage Phoenix cells (ATCC) at 90% confluence using Lipofectamine3000 (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... were dissolved in 100 μL isopropanol and 5 μL of each sample was applied to a C8 column (Accucore C8 column, 2.6 µm particle size, 50 x 2.1 mm, Thermo Fisher Scientific) at +40°C ...
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bioRxiv - Biochemistry 2024Quote: ... To separate phospho from non-phospho LRRKtide the following HPLC protocol was used: Hypersil Gold C18 (2.1×150 mm; 3 µm particle size, Thermo Scientific), Buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... lipids were separated on a C8 column (Accucore C8 column, 2.6 µm particle size, 50 x 2.1 mm, Thermo Fisher Scientific) mounted on an Ulitmate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Single-particle cryo-EM data of the stabilized ectodomain were acquired on a Glacios transmission electron microscope (Thermo Fisher Scientific) operating at 200 kV ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we incubated samples in the dark overnight and performed analysis of particle fluorescence and size on an Attune NxT V6 flow cytometer (ThermoFisher). We removed particles with extreme size or shape from the dataset ...
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bioRxiv - Cancer Biology 2023Quote: ... ITS (5 μg/ml insulin, 5 μg/ml transferrin, and 5 ng/ml sodium selenite, Gibco), and antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin) ...
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bioRxiv - Molecular Biology 2021Quote: ... wild-type or mutant IPMK cDNAs were subcloned into pFASTBAC1 plasmid (Thermo Fisher Scientific) with an N-terminal FLAG epitope ...
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bioRxiv - Developmental Biology 2020Quote: ... The paraffin embedded tissue was sectioned in Richard-Allan Type 9 paraffin (Thermo Scientific). Tissue was sectioned at 5 μm thickness ...
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bioRxiv - Cell Biology 2019Quote: ... or an untransfected wild type HEK Flp-in cell line (HEK wt, Invitrogen, R75007) were cultured in high-glucose DMEM (HyClone ...
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bioRxiv - Genetics 2019Quote: The total RNA of each organs type was extracted using Trizol reagent (Invitrogen, USA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Tissues were digested overnight in 0.1% collagenase Type I in PBS (Gibco, 17100-017) at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Other activation types assessed include: Dynabeads (1:1; 200,000/well) (ThermoFisher; Waltham, MA USA), Dynabeads (1:10 ...
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bioRxiv - Biophysics 2020Quote: Wild-type or truncated UMOD cDNA constructs were cloned in pcDNA3.1(+) (Thermo Fisher Scientific) and HA- or FLAG-tags were inserted after the signal peptide ...
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bioRxiv - Bioengineering 2020Quote: ... Chondrocytes were isolated by overnight digestion with 2 mg/mL Collagenase Type I (Gibco) for 14 h at 37 °C ...
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bioRxiv - Systems Biology 2020Quote: ... wild-type mouse NIH3T3 fibroblasts were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) complemented with 10% Foetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... a mixture of rat tail collagen type I (Cultrex) and Matrigel™ (Life Technologies) was mixed with CMs and gamma-irradiated human foreskin fibroblasts (HFFs ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were passaged with 1 mg/mL collagenase [(Type IV, Thermo Fisher Scientific, 17104019) in Gibco KnockOut DMEM/F-12 medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Fluorescently labeled wild-type PCSK9 was prepared using the DyLight800 Antibody Labeling Kit (ThermoFisher) as per manufacturer’s instructions followed by gel filtration chromatography on a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Bioengineering 2019Quote: Droplets were created with deionized (DI) water (Type II, Harleco; Fisher Scientific, Hampton, NH) tinted with either yellow or green dye (Spice Supreme ...