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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... After 7 days the spheroids were incubated with DRAQ5 (62254, Thermo Fisher Scientific, Waltham, MA, USA) at 1μM for 15 minutes prior to imaging as described (50) ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized (Maxima H cDNA kit) and qPCR was performed (QuantStudio 7 flex, Applied Biosystems). Primers used for gene expression analysis were ...
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bioRxiv - Cell Biology 2022Quote: COS-7 (ATCC® CRL-1651™) cells were grown in DMEM medium (Thermo Fisher Scientific) supplemented with 10% FBS (fetal bovine serum ...
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bioRxiv - Cell Biology 2022Quote: ... MCF-7 cells were also supplemented with 1x NEAA (non-essential amino acids) (#11140-035, Gibco). Human male diploid lung WI-26 SV40 fibroblasts (WI-26 cells ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: COS-7 cells were transiently transfected with the relevant DNA constructs using Lipofectamine 2000 (Invitrogen, 11668). Cell lysates (1 mg ...
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bioRxiv - Synthetic Biology 2022Quote: ... following manufacturer’s recommendations in triplicate on a QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher). Primer sequences are listed in Supplementary File 2 – Primer List.
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bioRxiv - Microbiology 2022Quote: ... 24 hours and 7 days were labelled with 0.5 μM BODIPY FL (Thermo Fisher, Waltham, MA), a hydrophobic dye that stains lipids ...
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bioRxiv - Plant Biology 2022Quote: RT-qPCR experiments were performed with three biological replicates using a QuantStudio 7 cycler (Applied Biosystems). The 10 μl reaction mixture contained 5 μl 2X SYBR Green/ROX master mix (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... The homogenate was then sonicated 10 times on setting 7 (Sonic Dismembrator Model 100, Fisher Scientific) and incubated at 4°C while rocking for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... All RT-PCR assays were done using the Viia 7 Real-time PCR System (Applied Biosystems). A standard curve was generated in order to obtain the exact number of copies in the tested sample ...
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bioRxiv - Synthetic Biology 2022Quote: ... The concentration of the transcription was measured as 7 µM using a Qubit RNA assay (ThermoFisher). A splint oligonucleotide (Z_Splint ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The RT-PCR reaction was performed using a QuantStudio™ 7 Flex system (Thermo Fisher Scientific) and data analysis was performed using the manufacturer’s web-based software (https://geneglobe.qiagen.com/analyze) ...
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bioRxiv - Molecular Biology 2022Quote: ... All qPCR analyses were performed on an Applied Biosystems QuantStudio 7 Flex Cycler (Thermo Fisher Scientific), and relative expression values were calculated using the comparative CT method (Schmittgen and Livak 2008).
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bioRxiv - Immunology 2022Quote: ... Thermal cycling and fluorescence detection were performed using the ViiA 7 real-time PCR system (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... and reactions performed on a ViiA− 7 Real-Time PCR System (all, Applied Biosystems, Waltham, MA). Data were acquired using the system’s RUO software and analyzed using the 2−ΔΔCT method ...
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bioRxiv - Developmental Biology 2023Quote: ... testicular tissues were digested with 7 ml collagenase type IV (290U/ml, Gibco cat # 17104-019) for 10 min at 37 °C with gentle agitation (250 rpm ...
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bioRxiv - Biophysics 2023Quote: ... and Septin-7 (VectorBuilder, Cat# VB220525-1339dzw) was performed in Opti-MEM (Gibco, Cat# 31985-070) using SuperFect reagent (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were run in a ViiA 7 Real-Time PCR system (Applied Biosystems, Foster City, CA) using fast reaction presets ...
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bioRxiv - Neuroscience 2023Quote: ... the hiPSCs were maintained for 7 days in a medium containing Neurobasal (Thermo Fisher Scientific, 12348017) and 1% neural induction supplement (NIS – Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... qRT-PCR were run in a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative quantification was determined using the ΔΔCt method and normalized to the endogenous controls GAPDH and RPLP0 ...
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bioRxiv - Molecular Biology 2023Quote: ... or 15 nM siRNA Control using 7 ul of Lipofectamine™ RNAiMAX transfection reagent (ThermoFisher Scientific) for each well according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was carried out using a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) and KAPA SYBR Fast qPCR reagents (KAPA Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Analysis of qPCR amplifications was performed with QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using the 2-ΔΔCT method with 36B4 serving as an internal control ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
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bioRxiv - Developmental Biology 2023Quote: COS-7 and HEK293 cells were grown in on plastic dishes in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was run in a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific 4453545) with cycle settings following manufacturer’s protocols for the RT-qPCR kit ...
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bioRxiv - Genetics 2023Quote: ... and 7 μM sections were cut using a cryostat onto Colorfrost Plus slides (Thermo Fisher Scientific). Sections were permeabilized in 0.25% Triton X-100 in PBS at room temperature for 12 min ...
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bioRxiv - Biochemistry 2022Quote: ... These SCX fractions (n=7) were cleaned up using C18 spin columns (89870, Pierce, ThermoFisher, USA), dried using SpeedVac at 40 °C and resuspended in formic acid (0.1 % ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using ‘SnakeSkin’ dialysis tubing with a 7 kDa molecular weight cut-off (ThermoFisher). His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time qPCR was performed in a QuantStudio 7 Flex real-time PCR system (Thermo Fisher) using iQ SYBR Green Supermix (Bio-rad #1708880 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytotoxicity of CellTrace™ Far Red-labelled tumor cells was measured by 7-AAD staining (Invitrogen) and flow cytometry ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) containing N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM; Invitrogen) at a final concentration of 10 μM and incubated for 30 min in the dark on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Integrin αV was stained with a commercial antibody (ThermoFisher, USA, clone RMV-7, 1:1000 diluted). Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech ...
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bioRxiv - Genetics 2022Quote: ... The samples were analyzed with the ViiA 7 Real-Time PCR System and software (Applied Biosystems).
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bioRxiv - Developmental Biology 2023Quote: ... RNA and H2Omq according to the protocol on ViiA 7 Real-Time PCR System (Applied Biosystems). Primer sequences used are specified in Suppl ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR reactions were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: COS-7 (monkey kidney fibroblast) cells obtained from ATCC (RRID: CVCL_0224) were cultured in DMEM (Gibco) with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... a 200mM stock of dopamine hydrochloride (Code 122000100 Lot: A0427132, CAS: 62-31-7, Acros Organics) in water was prepared ...
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bioRxiv - Neuroscience 2023Quote: For lightsheet experiments 6-7 dpf fish are placed in 2.2% low-melting point agarose (Thermofisher) in a chamber optimized for our lightsheet microscope.
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bioRxiv - Synthetic Biology 2023Quote: Huh-7 cells were seeded at 10,000 per well in glass-bottom chamber slides (ThermoFisher; 155409) and incubated overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... Huh-7 and TCMK-1 cells were maintained in high glucose DMEM media (Gibco, 41966-029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction was carried out in QuantStudio 7 Flex Real-Time PCR System (Invitrogen Biosciences). All the primer sequences were published previously (Liddelow et al. ...
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bioRxiv - Cell Biology 2023Quote: COS-7 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... The dispersion was transferred into a dialysis bag with a MWCO of 7 kDa (ThermoFisher, USA) for dialysis against deionized water to remove organic solution and stored at 4 C° for further use ...
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bioRxiv - Neuroscience 2023Quote: ... All qPCR reactions were run in triplicate and used SYBR-green on a QuantStudio 7 (ThermoFisher). The 2^(-ΔΔCt ...
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bioRxiv - Biochemistry 2023Quote: ... 7 mM NaOH) followed by an incubation of 5 hrs with Dynabeads Protein G (Invitrogen, 10004D). Beads were then washed with Wash Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: The samples were run using an Applied Biosystems VIIA-7 Real Time PCR system (ThermoFisher Scientific) using FastStart universal SYBR green master mix (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using the QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). The setting of the cycles used was the default for comparative Ct studies ...
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bioRxiv - Molecular Biology 2023Quote: ... CD19 and 7-AAD according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Pathology 2023Quote: ... RT-qPCR reactions were conducted on the Viia 7 Real-time PCR System (Applied Biosystems, USA) with SYBR Premix Ex TaqTM II (TaKaRa ...