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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... followed by a 1 h incubation at room temperature with a 1:5,000 dilution of secondary anti-rabbit-HRP antibody (Invitrogen) in TBST ...
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bioRxiv - Biophysics 2020Quote: ... the double-cysteine mutant of ClpB (or ΔNClpB) was incubated for 1 h with 1:1.2 molar ratio of Alexa Fluor (AF) 594 (C5 maleimide, Invitrogen) under native conditions (25 mM HEPES ...
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bioRxiv - Bioengineering 2020Quote: ... Actin filaments were fluorescently labeled with AlexaFluor-488-conjugated phalloidin (Life Technologies; 1: 200 dilution, 1 h at RT) for visualization ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were incubated for 1 h at room temperature with Alexa-dye-conjugated secondary antibodies (1:1000; Invitrogen) in a blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were incubated for 1 h at room temperature with Alexa-dye-conjugated secondary antibodies (1:1000; Invitrogen) in a blocking solution ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Electroporated slices were placed for 1 h in MEM medium (10% FBS, 0.5% glucose, 1% Pen/Strep, in MEM with glutamine, all Gibco) in a 5% CO2 humidified incubator at 37 °C and then incubated in Neurobasal/B27 medium for 18-24 h followed by the time lapse imaging (Denaxa et al. ...
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bioRxiv - Genetics 2022Quote: ... Sheep IgG (H+L) Highly Cross-Adsorbed Donkey anti-Ovine HRP (1:2000-1:6000, ThermoFisher Scientific, catalogue #A16047)
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer), and subsequently washed four times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer) and washed four times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were continued for another 3.5 h prior to addition of 1 µM NAO and/or 1 µM ReAsH-EDT2 reagent (Invitrogen). Cultures were incubated for 1 h at room temperature and washed with 1X BAL wash buffer per manufacturer kit instructions ...
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bioRxiv - Neuroscience 2023Quote: ... then subjected to a 1 h incubation with secondary antibody (Goat anti-Rabbit IgG, 1:200, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated for 1 h at room temperature with Alexa-dye-conjugated secondary antibodies (1:1000; Invitrogen) in a blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in TBS-T and further incubated for 1 h with goat anti-rabbit at 1:3,000 (ThermoFisher) and goat anti-mouse (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were successively incubated with indicated primary antibodies in PBS/BSA 1% saponin 0.1% for 2 h at RT and with Alexa Fluor-conjugated secondary antibodies in PBS/BSA 1% saponin 0.1% for 1 h raised against mouse or rabbit immunoglobulins (Invitrogen). After washes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated with 1 µM Rhonox-M for 1 h or lysotracker deep red (Thermo Fisher Scientific L12492) according to the manufacturer’s instructions for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... anti-rabbit HRP antibody (1:5000) or with anti-goat HRP antibody (1:5000) was performed for 1 h and ECL (Pierce, Thermo Scientific, Rockford, IL, USA) visualized detection ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:200 dilution in 1% BSA/PBS for 1 h at RT followed by incubation with streptavidin-conjugated 488 (Thermo Fisher Scientific, Cat. # S11223) at 1:1000 dilution in PBS for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were stained for 3 min with Hoechst (Invitrogen, Cat #H-3570). Cells were then analyzed by confocal microscopy on a Zeiss LSM700 inverted microscope.
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bioRxiv - Molecular Biology 2021Quote: ... cells were selected with 1 μg ml-1 and 2 μg ml-1 puromycin (Thermo Fisher Scientific) respectively for 10 days.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge, Thermo Fisher Scientific, Darmstadt, Germany). The fluorescence intensity of the supernatant was measured measured (485 nm excitation ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM Mg(OAc)2 (Invitrogen), 0.55 mM spermidine (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... at a ratio of 1:1.5:1.75:2 (FAM155A-3×FLAG:NALCN-1077-3×HA-GFP:UNC79-3×FLAG:UNC80-3×FLAG) using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 40-48 hours ...
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bioRxiv - Plant Biology 2020Quote: General ROS were detected using 2’-7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA, Invitrogen). CM-H2DCFDA was dissolved in DMSO to give a concentration of 1 mM and further diluted to a final concentration of 50 μM in water ...
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bioRxiv - Cell Biology 2022Quote: The cell-permeant reagent H2DCFDA (2’, 7’-dichlorodihydrofluorescein diacetate) (Thermo Fisher Scientific) was employed to represent the ROS levels in HeLa cells ...
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bioRxiv - Cancer Biology 2020Quote: ... for 7 minutes at 20 V using iBlot 2 (Thermo Fisher Scientific). Blots were blocked in 5% dried milk (AppliChem ...
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bioRxiv - Neuroscience 2021Quote: ... or 10 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA; Thermo Fisher Scientific #D399), an MRP family substrate ...
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bioRxiv - Cell Biology 2022Quote: ... + 1.6 mM Tris pH 7) and mounted in 1.2-2% agarose (Invitrogen) in 35mm #1.5 glass-bottom dishes (CellVis D35-20-1.5N and D35C4-20-1.5N) ...
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bioRxiv - Cell Biology 2022Quote: ... + 1.6 mM Tris pH 7) and mounted in 1.2-2% agarose (Invitrogen) in 35mm #1.5 glass-bottom dishes (CellVis D35-20-1.5N) ...
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bioRxiv - Molecular Biology 2023Quote: ... by the iBlot 2 transfer system (Invitrogen, 20 V for 7 min). The membranes were blocked with 3% nonfat milk or ECL PrimeTM blocking agent (Cytiva ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Physiology 2024Quote: ... Tim-4 (1:1000 dilution, rabbit anti-TIM-4 antibody, Invitrogen, PA5-116045, RRID:AB_2900679) and Lumican (1:1000 dilution ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM 3-methyl-2-oxobutanoic acid (Fisher Scientific, Hampton, NH) and 1 mM acetyl-CoA (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 1 - 2 μg ml-1 poly(dA:dT) (InvivoGen) (transfected with Lipofectamine 2000, Invitrogen), 100 µM imiquimod (R837 ...
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bioRxiv - Neuroscience 2023Quote: ... containing 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Cultured neurons were fed with 250 μl NB+ media on days in vitro (DIV ...
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bioRxiv - Evolutionary Biology 2023Quote: ... supplemented with 1/2 x N2 and 1 x B27 (Thermo Fisher Scientific), 1% Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... and then loaded with fluorescent Ca2+ probes (3 μM of fura-2 AM or 5.69 μM of Fluo-4 AM, Life Technologies) in HBSS for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with RNAScope H2O2 for 4 min at RT and subsequently washed 2 x 3 min with UltraPure Distilled Water (Invitrogen) at RT ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Developmental Biology 2021Quote: ... were crossed for 2-3 days and then transferred to fly food made from 0.6 g of Carolina Formula 4-24 (Fisher Scientific) and 2 mL of ddH2O ...
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bioRxiv - Biophysics 2022Quote: ... N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (NBD-PE) was purchased from ThermoFisher (Waltham, MA). Cholesterol Oxidase from Streptomyces was purchased from MP Biomedicals (Santa Ana ...
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bioRxiv - Genomics 2024Quote: ... and transfer plasmids were transfected at a mass ratio of 2:3:4 with Lipofectamine 3000 (Thermo Fisher Scientific L3000001) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Neuroscience 2021Quote: ... we re-suspended cells in PBS/ 0.2% human serum containing 2 µg/mL 7-aminoactinomycin D (7-AAD) (Invitrogen, Cat# A1310). We carried out isotopic controls with irrelevant mouse IgG1-APC ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TIGIT PE Cy 7 (1:50, clone MBSA43, Invitrogen, cat # 25-9500-42), Anti-human CD94 APC (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 µl of ERCC RNA spike-in mix at 10:7 dilution (Life Technologies) and barcoded reverse transcription (RT ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Amphiregulin PE Cy 7 (1:25, clone AREG559, Invitrogen, cat # 25-5370-42), Anti-mouse Nur77 APC (1:25 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT) in 7 kDa Slide-A-Lyzer MINI Dialysis Devices (ThermoFisher Scientific, 69562). Samples were placed in 400 ml high-salt buffer ...