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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... All drug treatments were performed for 3 – 5 hours prior to imaging with a final concentration of 5 nM of actinomycin D (Gibco, 11805-017) or 1 µM of BMH-21 (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were plated in 24-well plates and the plasmids (psi-check-2-WT, psi-check-2-mut) were transfected using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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Trade-offs between antiseptic cytotoxicity and efficacy in a human ex vivo wound contamination modelbioRxiv - Microbiology 2021Quote: ... each bisect was rinsed in PBS and placed in an individual well of a 6-well plate with 2 mL MTT solution (2 mg/mL, Invitrogen) in each well ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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bioRxiv - Genomics 2020Quote: ... based on Sanger sequencing method using BigDye™ Terminator v3.1 cycle sequencing kit (Applied Biosystems, Thermo Fischer Scientific, Inc.). Sequencing was done using 3500xl genetic analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... the shRNA construct was obtained by cloning the sequence into pcDNA6.2-GW/EmGFP-miR plasmid using a microRNA (miR)-based expression vector kit (BLOCK-iT Pol II miR; Invitrogen), thereby creating an expression cassette consisting of the 5′ miR flanking region ...
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bioRxiv - Genomics 2021Quote: ... based purification of cDNA and quantification using Qubit dsDNA HS assay kit (Cat. No. Q32854, Invitrogen, Carlsbad, California, USA).
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bioRxiv - Developmental Biology 2024Quote: ... In vitro transcription (IVT) of the sgRNA-based PCR template was performed using the MAXIscript T7 Kit (AM132, Ambion) with an overnight reaction run at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... YFP-RPB1-26xCTD and T4E/S7E mutants were generated by PCR-based cloning performed by a kit from ThermoFisher. For HeLa cell transfection & imaging ...
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bioRxiv - Bioengineering 2023Quote: ... Viable cells were selected based on the staining with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Thermo Fisher) prior to the Fc-blocking.
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bioRxiv - Neuroscience 2024Quote: ... the samples underwent normalization based on protein content using the BCA protein assay kit (#23225, Thermo Scientific Pierce, US) per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed thrice with PBS-T and imaged with an enhanced luminol-based chemiluminescent substrate kit (Thermo Fisher) on a gel imager (Bio-rad).
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bioRxiv - Cell Biology 2024Quote: ... followed by Trizol based extraction of total cellular RNA using the Purelink RNA extraction kit (Invitrogen, cat. no. 12183018A). cDNA conversion of the cellular mRNA was performed using SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Molecular detection of SARS-CoV-2 was performed with TaqMan™ Gene Expression Assay (Cat. N. 4331182, ThermoFisher®) with specific primers/probes previously described40 ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The immuno-detection of proteins was carried out using mouse anti-HA monoclonal antibody (2-2.2.14, #26183, ThermoFisher Sci) and secondary anti-mouse IgG antibodies conjugated with horseradish peroxidase (#A9044 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with each sample being prepared from 5 individuals (3 karyotypes x 2 temperatures x 9 isochromosomal lines = 54 samples) using the MagMAX™-96 Total RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) on a MagMAX™ Express Magnetic Particle Processor (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized by saponin-based permeabilization buffer (Thermo Fisher) for 15 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... based on Nanodrop® 1000 (Thermo Fisher Scientific), were analysed on a 1% agarose gel ...
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bioRxiv - Plant Biology 2020Quote: Vectors based on the Gateway cloning technology (Invitrogen) were used for all manipulations ...
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bioRxiv - Plant Biology 2020Quote: ... A capillary-based ABI3730xl sequencing system (Applied Biosystems) at the sequencing facility of IPK was used to separate the fluorescently terminated extension products ...
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bioRxiv - Microbiology 2021Quote: ... and normalised based on Quant-iT PicoGreen (Invitrogen) quantifications ...
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bioRxiv - Neuroscience 2021Quote: ... gateway recombination-based cloning was performed (Life Technologies) using the Tol2kit99 ...
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bioRxiv - Microbiology 2021Quote: ... a plasmid-based promoter fusion from Thermo Scientific E ...
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bioRxiv - Microbiology 2021Quote: ... and normalised based on Quant-iT PicoGreen (Invitrogen) quantifications ...
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bioRxiv - Immunology 2021Quote: ... RRID:AB_465322) based on the isotype control (Thermo Fisher Scientific Cat# 11-4888-81 ...
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bioRxiv - Molecular Biology 2021Quote: ... and the lipid-based transfection reagent RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: A pDONR223-based entry clone (Thermo Fisher Scientific) containing the sequence encoding canonical full-length BIRC6 (aa1-4857 ...
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bioRxiv - Neuroscience 2022Quote: ... Glasgow’s MEM (GMEM)-based (Gibco, Cat. no. 11710035) CDMI includes 20% KnockOut Serum Replacement (KOSR ...
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bioRxiv - Microbiology 2023Quote: ... Sample concentrations were normalized based on PicoGreen (Invitrogen) quantification and pooled together ...
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bioRxiv - Microbiology 2023Quote: ... then subjected to DNAzol-based DNA extraction (Invitrogen). Extracted midgut DNA was amplified using Plasmodium genus-specific primers in a conventional PCR as a first round reaction [25] ...
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bioRxiv - Plant Biology 2023Quote: Vectors based on the Gateway cloning technology (Invitrogen) were used for all manipulations ...
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bioRxiv - Immunology 2023Quote: ... Phalloidin-based F-Actin (Thermofisher, cat no: A30107) staining was performed using BD Perm/Wash reagent (BD ...
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bioRxiv - Cancer Biology 2024Quote: ... SYBR-green based RT-qPCR (Applied Biosystems, 4472908) was performed using CFX96 Real-Time System ...
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bioRxiv - Developmental Biology 2021Quote: A dam at E16.5 in gestation was given an intraperitoneal (IP) injection with EdU (5-ethynyl-2’-deoxyuridine) from the Click-iTTM EdU Alexa FluorTM 647 Imaging Kit (Invitrogen, Carlsbad, CA, USA) at a dosage of 0.25mg /10g body weight ...
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bioRxiv - Developmental Biology 2020Quote: Cumulative curve of the thymidine analog 5-Ethynyl-2’-deoxyUridine (EdU) incorporation is performed using Click-iT™ Plus EdU Alexa Fluor™ 647 Imaging Kit (ThermoFisher; C10640). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... 5 μg of each RNA sample was incubated twice with 2 units of TURBO DNase (TURBO DNA-free Kit, Ambion, Austin, TX, USA) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: Proliferation of pericytes was determined by incorporation of the thymine analogue 5-ethynyl-2’-deoxyuridine (EdU) with the Click-iT®Assay Kit (Life Technologies C10340) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Cell cycle changes were additionally assessed using the 5-ethynyl-2’-deoxyuridine (EdU) assay using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10418). HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher ...
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bioRxiv - Cancer Biology 2019Quote: ... T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher, C10423) in 96 well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Immunology 2021Quote: ... Detection was performed using the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Chemiluminescent detection was performed with ECL western blotting detection reagent (Westdura, Thermo Scientific) using FluoroChem 8800 ...
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bioRxiv - Plant Biology 2023Quote: ... Signal detection was performed using the Chemiluminescent Nucleic Acid Detection Module (ThermoFisher Scientific). Images were taken using Amersham AI680 Western blot Imaging System (Cytvia).
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bioRxiv - Genetics 2019Quote: ... HEK293T cells were grown to 70-90% confluency in a 6-well plate and then transfected with 5 µg of the constructed DNA (or 5 µL ddH2O) using Lipofectamine 3000 (ThermoFisher Scientific; L3000008). 3.75 and 7.5 µL of Lipofectamine 3000 reagent and 10 µL P3000 reagents were used for transfection ...
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bioRxiv - Microbiology 2019Quote: ... Strains were routinely grown at 37°C and 5% CO2 overnight on tryptic soy agar plates supplemented with 5% sheep blood (TSAII) (Thermo Fisher Scientific). Growth in broth culture was performed in BHI (Becton ...
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bioRxiv - Biophysics 2021Quote: ... 5 % CO2 incubator for 5 hours before replacing the culture media with 2 mL B-DMEM (Thermofisher, cat. # 10566016) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were rinsed 2 times for 5 minutes with PBS++ and incubated with 5 drops of NucBlue (Life Technologies) for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... A single bolus of 50μL 0.5% Texas-red dextran solution (70,000 MW, 5 mg/mL in 0.9% NaCl, t1/2 ∼ 25min, Termo Fisher Scientific) was injected ...
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bioRxiv - Neuroscience 2023Quote: ... A 50μl bolus of 0.5% Texas-red dextran solution (70,000 MW, 5 mg/mL in 0.9% NaCl, t1/2 ∼ 25min, Termo Fisher Scientific) was injected at a constant rate of 30μl/sec using a syringe infusing pump (GenieTouch ...