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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Sections were cut (4 µm) using a cryostat and labelled overnight at 4°C with COL6 antibody (1:500) (MA5-32412, Thermo Fisher Scientific, USA) after blocking nonspecific binding sites with 10% bovine serum albumin (A7906 ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C and normalized by BCA (Thermo Fisher Scientific) using BSA as standard ...
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bioRxiv - Microbiology 2020Quote: ... overnight at 4°C and donkey anti-rabbit (ThermoFisher) (1:400 ...
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bioRxiv - Microbiology 2021Quote: ... at 4°C (Nunc MaxiSorp flat bottom, ThermoFisher Scientific). Coated plates were blocked with PBS containing 5% low-fat milk and 1% BSA for 1 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... with 4 °C cold phosphate buffered saline (PBS, Gibco) and centrifuged at 4 C and 300 g ...
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bioRxiv - Synthetic Biology 2024Quote: ... digested with AarI (Thermo Fisher; 4 h, 37 °C) and ligated into the previously digested backbone plasmid using a T4 ligase (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... we loaded the cells with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Molecular Probes®) and incubated at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were stained with 2’,7’-Dichlorofluorescein diacetate (10 µM) (Thermo Fisher, USA) for one hour ...
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bioRxiv - Molecular Biology 2022Quote: ... then placed in 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (Invitrogen, Carlsbad, CA, USA) and incubated under vacuum for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... follicles were treated with 10μM of 2′,7′ dichlorodihydrofluorescein diacetate (H2DCFDA) (Invitrogen- D399) for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5A for 7 min using iBlotTM 2 dry Blotting Transfer system (Thermo Fisher). The membrane was blocked in Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... The GST-fused protein was eluted with 1 ml GST elution buffer and the eluted sample was dialyzed overnight at 4°C in 2 l 1x PBS in a Slide-A-Lyzer Dialysis Cassette G2 10 000 MWCO (Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Jurkats were treated with 150mJ of UV energy and incubated for 2-4 hours at 37°C in complete media consisting of RPMI 1640 Media (Gibco, 11875101), 10% FBS (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of the polar phase was collected and vacuum-dried at 4°C and subsequently derivatized with 2% (w/v) methoxyamine hydrochloride (Thermo Scientific) in pyridine for 60 min following by 30 min sialyation N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed for 15 min at 37°C in 4% paraformaldehyde and incubated with 2 μM Bodipy 493/503 (Bodipy 493, Molecular Probes) and 1 μg/ml of DAPI as published (9) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Physiology 2022Quote: ... The corpus was incubated in 50% betaisodona/PBS (Mundipharma GmbH) for 30 min at 4°C before being washed in different solutions for 2 min each: PBS (ThermoFisher Scientific), 0.1% octenidin in ddH2O (Serva Electrophoresis) ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20) replacing the buffer every 2 hours and incubated overnight at 4°C with the appropriate secondary antibodies (ThermoFisher Scientific), anti-Rabbit-Alexa 488 (RA3) ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Systems Biology 2020Quote: Similar to our recent report,16 1 mg nuclear protein extracted from Raw 264.7 macrophage cells was incubated overnight at 4°C with 2 nmole UNC0965 pre-coupled to 50 μl neutravidin-agarose (Thermo Fisher), and washed three times with 1 ml lysis buffer to remove non-specific bound proteins ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Developmental Biology 2020Quote: ... while the remainder was immunoprecipitated for 2 hr at 4°C in the following antibodies: rabbit anti-GFP (Invitrogen, A-11122), rabbit anti-Cen ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 ul/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in FACS buffer (PBS with 2% newborn calf serum ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VIPR particles were centrifuged at 14,500 xg for 3 minutes at 4°C and washed three times in PBS while the beads were immobilized in an Invitrogen DynaMag-2 magnet (Thermo Scientific). VIPR particles were resuspended in sterile PBS at a concentration of 5×108 particles per ml and stored at 4°C.
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 50 μL were incubated at 4°C for 2 h with 40 μg of pre-equilibrated HisPur Ni–NTA magnetic beads (Thermo Scientific) in a final volume of 200 μL ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded for 2 h at 4°C on a bi-directional rotator with 2 mM Ca2+ medium containing drugs 30 μM pancuronium (Fisher Scientific), or 38.9 μM curare (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Cells were loaded at 37°C for 60 minutes with 4 μM Fura-2-AM in the presence of 1X Power Load permeabilizing reagent (Thermo Fisher). Injection of AngII (10-8M) ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... (3) 65%-95% for 5 min and (4) washout for 15 min) using the EASY-nano LC 1200 system (Thermo Fisher Scientific) with a flow rate of 300 nL/min ...
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bioRxiv - Bioengineering 2024Quote: ... and mixed with 3 µ L of DMRIE-C (Invitrogen). After incubation at room temperature for 45 min ...
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bioRxiv - Bioengineering 2022Quote: ... The 24-well plates with infected BHK-21 cells were overlaid with 1 ml of 0.8% methylcellulose in complete DMEM medium with 2% FBS and incubated for 5 days in a cell culture incubator (Thermo Fisher Scientific, 37°C and 5% CO2). Plaques were fixed and developed with staining reagent (1% crystal violet in 1:1 methanol/acetone solution ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular 7-ketocholesterol was measured by fixing cells in 4% paraformaldehyde and staining with 7-ketocholesterol monoclonal antibody at 1:50 (3F7, Invitrogen, MA5-27561) diluted in 1X permeabilization buffer (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... + 2% heat inactivated fetal bovine serum Certified One Shot (Gibco A38400-01) + 1× N-2 supplement ...
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bioRxiv - Genomics 2021Quote: ... on the Ion One Touch™ 2 System (Thermo Fisher Scientific, USA). Template-positive ISPs were enriched on Ion One Touch™ ES system (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2024Quote: ... coli One Shot™ ccdB Survival™ 2 T1R Competent Cells (ThermoFisher). Single colonies were cultured for plasmid isolation and the cloned sequence was confirmed by DNA sequencing.
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bioRxiv - Microbiology 2022Quote: ... coli One Shot® ccdB SurvivalTM 2 T1R competent cells (Life Technologies), which was grown in LB agar plates (10 g l-1 NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated overnight at 4°C with the following primary antibodies at a concentration of 1:500: mouse anti-V5 (1:500, Invitrogen), rabbit anti-γ-H2AX (1:500 ...
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bioRxiv - Bioengineering 2019Quote: ... overnight at 4°C followed by AlexaFluor-568 (1:500, Invitrogen, A21099 and AlexaFluor-647 antibody (1:500, Invitrogen, A31573) respectively ...
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bioRxiv - Neuroscience 2020Quote: ... (iii) incubated overnight at 4 °C in the following affinity-purified rabbit KV7.2 (residues 1–70; 1:1000, Thermo Scientific), KV7.3 (residues 668–686 ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 1 mL of cold 1 X PBS (4°C) containing protease inhibitor (ThermoFisher; cat# 78429) and were transferred into tubes containing lysing matrix B (MP Biomedicals ...
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bioRxiv - Developmental Biology 2022Quote: ... with 0.1% (w/v) Tween20 and incubated overnight with primary antibodies at 4° C: anti-HIF1α (1:500, MA1-16504 Invitrogen); anti-STAT3 (1:1000 ...