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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were transfected with pX330 containing the target site using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Knockout clones were obtained by limiting dilution and KO confirmed by sequencing the target sites in the genome DNA and by flow cytometric analysis of GPI-APs ...
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bioRxiv - Immunology 2020Quote: ... HEK293 T cells were transfected with lipofectamine 3000 following the manufacturer’s procedure (Invitrogen, Carlsbad, CA, USA). Prefusion F (BV2129 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were transfected with the indicated expression vectors using Lipofectamine 3000 (Life Technologies L3000-015). 48 h after transfection ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the control and ADARB1 encoding pcDNA3 constructs using Lipofectamine 3000 (Invitrogen) and analyzed 72hrs post-transfection.
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral supernatants were generated by transient transfection of HEK293 cells according to Lipofectamine ™ 2000 (Invitrogen) transfection protocols and harvested 48 h after transfection ...
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bioRxiv - Genomics 2022Quote: HUVECs and pB-circRNA transfected HEK293 were grown in media supplemented with exosome-depleted (Life Technologies). HEK293 were induced with 3 μg/ml doxycycline (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... BAC DNA was transfected into HEK293/ACE2/TMPRSS2 cells using lipofectamine 3000 (Thermo Fisher, cat. L3000001). Two days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: Culturing of HEK293 (RRID:CVCL_0045) and HeLa cells (RRID:CVCL_0030) and their transfection using TurboFect (Thermo Fisher Scientific) was essentially done as described (Kessels et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific, Cat. #11995040). pCDH-EF1a-mCherry-EGFP-LC3B (RRID ...
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bioRxiv - Physiology 2024Quote: ... These cells were generated by transfecting HEK293 cells using Lipofectamine 2000 (Invitrogen; 2 μl per well) with D1ER cDNA (0.5 μg/well ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
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bioRxiv - Biophysics 2023Quote: 15 mL of HEK293 cells were plated at 0.625 × 106 cells / mL in DMEM (Gibco 11995065) supplemented with 10% FBS in a T75 flask ...
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bioRxiv - Biophysics 2023Quote: 15 mL of HEK293 cells were plated at 0.625 × 106 cells / mL in DMEM (Gibco 11995065) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: COS-7 and HEK293 cells were grown in on plastic dishes in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Hek293 Flp-In T-REx cells were additionally supplemented with 100 µg/ml hygromycin B (Invitrogen) and 10 µg/ml blasticidine (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected at 70 to 80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015) and incubated at 37°C for 2 days ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC;CRL-1573) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965-092) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Genetics 2023Quote: ... HEK293 cells were transfected with PX459 and pCR-Blunt II TOPO plasmids by Lipofectamine 3000 (Invitrogen) for 72 hr ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321 ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa RIEP (Jurkin et al. 2014) and HEK293 Flip-In™ T-REx™ (FITR, Invitrogen) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM high glucose ...
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bioRxiv - Neuroscience 2023Quote: HEK293S GnTIˉ cells (ATCC CRL-3022) were cultured in FreeStyle™ 293 Expression Medium (Gibco, 12338018) containing 1% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 and Lenti-X 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 and Lenti-X 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... both plasmids containing sgRNAs were transfected into naïve HEK293 cells by Lipofectamine 3000 (Thermo Fisher Scientific) as instructed by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting constructs were transfected into HEK293 cells using the Lipofectamine 3000 Reagent (Invitrogen, Cat#L3000001) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using ABI7900HTFast (Thermofisher, Waltham, MA). The data were normalized to the β-actin expression level and are expressed as the fold change relative to control (2−ΔΔCt).
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bioRxiv - Molecular Biology 2021Quote: ... Polymerase chain reactions (PCR) were prepared with Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific). PCR products were purified with the Wizard SV Gel and PCR Cleanup System (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... Pooled sequencing libraries were quantified with digital polymerase chain reaction (PCR) (QuantStudio 3D, Thermo Fisher) and sequenced on an Illumina HiSeq 1500 platform ...
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bioRxiv - Neuroscience 2020Quote: ... Expression of specific genes was assessed using quantitative Real Time Polymerase Chain Reaction (Applied Biosystems). Each qPCR reaction used 100 ng of cDNA with QuantiTec primers for tumor necrosis factor α (TNFα) ...
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bioRxiv - Immunology 2020Quote: ... Heavy and light chain sequences of the b12 antibody were ordered at GeneArt (Thermo Fisher). All sequences were verified using Sanger sequencing.
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bioRxiv - Immunology 2021Quote: ... the corresponding heavy and light chain plasmids were chemically co-transfected into ExpiCHO cells (Gibco) and transiently expressed for 9 days ...
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bioRxiv - Biochemistry 2022Quote: ... Polymerase chain reaction was carried out using Phusion U Hot Start polymerase (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Immunoglobulin epsilon (ε) heavy chain transcripts were amplified (StepOnePlus Real-Time PCR System, Applied Biosystems) using custom TaqMan gene expression kits (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... real-time polymerase chain reaction was performed with the 7900HT Sequence Detection System (Applied Biosystems) using the KAPA PROBE FAST Universal qPCR kit (Peqlab) ...
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bioRxiv - Bioengineering 2022Quote: ... Quantitative reverse transcription–polymerase chain reaction (qPCR) was performed with PowerUp SYBR green (ThermoFisher Scientific) using the StepOne Plus system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Polymerase chain reaction (PCR) was performed with Phire Hot Start II DNA polymerase (Thermo Scientific), Phusion High-Fidelity DNA Polymerase ...
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bioRxiv - Plant Biology 2022Quote: ... The polymerase chain reaction (PCR) utilizing Phusion© polymerase enzyme (Thermo Fisher Scientific;Darmstadt, Germany) was used to amplify specific DNA fragments by using gene specific primer pairs depicted in Table S1.
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bioRxiv - Physiology 2022Quote: ... Quantitative polymerase chain reactions (qPCRs) contained 1X PowerUp SYBR Green PCR Master Mix (Applied Biosystems), 500 nM appropriate primers ...
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bioRxiv - Plant Biology 2024Quote: ... Polymerase chain reaction (PCR) was performed using PhusionTM High-Fidelity DNA Polymerase (ThermoFisher Scientifc, USA), the bands of desired sizes were gel-purified using Promega Wizard® SV Gel and PCR Clean-Up System (Promega ...
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bioRxiv - Immunology 2023Quote: ... 12 μg plasmid of each chain were mixed into 3 ml Opti-MEM (Gibco 31985070), followed by adding 24 μl FectoPRO transfection reagent (116-040 ...
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bioRxiv - Physiology 2023Quote: Quantitative real-time polymerase chain reaction was performed using Power SYBR Green (Thermo Fisher Scientific) and an optical 384-Well Reaction Plate (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... J chain Western blots were performed using iBlotTM 2 Dry Blotting System (Thermo Fisher Scientific) and iBindTM Flex Western system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... antibody heavy and light chain expression vectors were transiently transfected into Expi293 suspension cells (ThermoFisher). For the generation of the human IgG1 afucosylated Fc-domain variant ...
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bioRxiv - Genetics 2023Quote: ... Polymerase chain reaction products were amplified using Phusion HS II DNA polymerase (F549; Thermo Fisher). Gibson Assembly was conducted using Gibson Assembly Master Mix (E2611 ...
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bioRxiv - Cell Biology 2023Quote: ... Cysteine side chains were alkylated by adding 5 mM Iodoacetamide (IAA, Thermo Fisher Scientific # A39271) and incubated in the dark for 20 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... NAb™ Protein L Spin Kit for all Kappa chain antibodies (all from Thermo Scientific). Purified intact antibodies (40 μg IgG ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was then used for quantitative Polymerase Chain Reaction (qPCR) using PowerTrack SYBR Green (ThermoFisher) with the pair of primers for mouse glyceraldehyde-3-phosphate dehydrogenase (mGapdh ...
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bioRxiv - Immunology 2019Quote: ... MRC5 human fibroblast cell line and A375 human melanoma cell line are cultured at 37°C under 5% CO2 in DMEM medium (Life Technologies) containing 1% glutamine ...
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bioRxiv - Genomics 2019Quote: ... were immortalized with human telomerase reverse transcriptase (hTERT) and cultured in complete AmnioMAX C-100 Basal Medium (ThermoFisher Scientific, Carlsbad, CA) supplemented with 15% AmnioMAX supplement (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...