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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... These fragments were inserted into the pIZ-V5/His vector (Invitrogen) using the In-FusionHD Cloning Kit (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... ceylanicum were subcloned into the pMT/BiP/V5-His vector (ThermoFisher) in frame with the BiP signal sequence and the C-terminal V5 and six-histidine tags ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were directly incubated with DyLight680-conjugated anti-His antibody (Thermofisher), washed ...
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bioRxiv - Microbiology 2020Quote: ... The His-MspGH25 cloned into pGAPZαA vector (Invitrogen, Carlsbad, CA, USA) under the control of a constitutive promotor with an α-factor signal peptide for secretion ...
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bioRxiv - Microbiology 2021Quote: ... using an Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions to ensure a high degree of sensitivity required and the deal with the high complexity of our samples under investigation.
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bioRxiv - Evolutionary Biology 2021Quote: ... suspended in Hi-Di formamide (Applied Biosystems, Foster City, CA, USA) and run on an ABI 3730 at the University of Georgia ...
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bioRxiv - Immunology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS, Invitrogen, 12484028). DMEM supplemented media consisted of 2 mM GlutaMAX ...
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bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...
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bioRxiv - Molecular Biology 2020Quote: ... The empty coding vector pcDNA3.1/MYC-His was obtained from Invitrogen and pCMV-β-Galactosidase was obtained from Clontech/Takara Bio.
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bioRxiv - Microbiology 2023Quote: ... and inserted into similarly digested pcDNA4-To/myc-His B (Invitrogen), resulting in pcDNA4-L3HYPDH ...
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bioRxiv - Microbiology 2023Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Hyclone and Gibco), and 100 U/mL penicillin/streptomycin (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... His-HcpGA1 was purified using the ProBond Purification System (Life Technologies) and the eluted protein was dialyzed against PBS and used as immunogen for antiserum generation.
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (HI-FCS, Gibco) and 1% of Penstrep (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
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bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) and 1% penicillin-streptomycin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Western blot analysis using anti-His antibody (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... 2 µL of Novex Hi-Density TBE 5X Sample Buffer (Invitrogen) was mixed with 8 µL of the completed EMSA reaction and loaded on an 8% acrylamide gel ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...
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bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Initialization was performed using the IonTorrent Hi-Q View Kit (Thermofisher) with Ion 318 Chip Kit v2 BC (Thermofisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in 10% fetal bovine serum (FBS - HI; Gibco) in Dulbecco’s modified Eagle’s medium (DMEM) ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco). The HEK 293T cell line was used to make lentiviruses and was cultured at 37°C with 5% CO2 in DMEM supplemented with 10% HI-FBS ...
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bioRxiv - Biochemistry 2024Quote: His-MKK4 was overexpressed in and purified from Sf9 (Thermo Scientific) cells ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were bled and total IgG (mouse and human) was pulled down using protein A beads (ThermoFisher, Catalog # 20333). An in–house sandwich ELISA was used to measure human IgG (control IgG or MS-Hu6 ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: cDNAs encoding human full-length of CREB3L3 (NM_032607.2) and green fluorescent protein were cloned into pENTR4 vectors (Life Technologies). In addition ...
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bioRxiv - Microbiology 2022Quote: ... acquired MS/MS spectra were searched against a human uniprot protein database in Proteome Discoverer 3.2 (Thermo Fisher Scientific) using Label free Quantification with Minora feature detection ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA coding for the protein sequences was synthesized using codon-optimization for human host cells (Thermo Fisher Scientific). If the codon optimization for the lysine-stretches resulted in six or more consecutive adenines in the DNA ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Microbiology 2023Quote: ... and cytokine concentrations were quantified with the Human Cytokine 16-Plex Protein Standard (Thermo Fisher Scientific, Waltham, MA, USA). For quantification of BPI in human sera ...
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bioRxiv - Microbiology 2024Quote: ... the S protein labelling was visualized with an Alexa-488-conjugated goat anti-human antibody (ThermoFisher, cat. No.: A11013), while for the N protein labeling an Alexa-488-conjugated goat anti-rabbit secondary antibody (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: The human BEST1 protein (spanning amino acids 1-398) was expressed in Pichia pastoris using the pPICZ vector (Invitrogen) with a C-terminal green fluorescent protein (GFP ...
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bioRxiv - Genetics 2024Quote: ... we obtained constructs which were codon-optimized for Drosophila and encoded the human protein from GeneARTTM (Thermo Fisher Scientific) (See Supplemental Text) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the media was changed and two days later the cells were collected and plated on gamma irradiated MEF feeders (Gibco, A34181) in human ES media (DMEM/F12 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Eight days after subcutaneous immunisation of C57BL/6 mice with 50 μg of 4-Hydroxy-3-nitrophenyl acetyl hapten conjugated chicken gamma globulin (NP-CGG) in Imject Alum Adjuvant (ThermoFisher Scientific), 4.3 µg MAR hypoxia detecting probe (Goryo Chemical ...
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bioRxiv - Microbiology 2023Quote: ... Cells were always normalized to their optical density at 750 nm using a Helios Gamma UV-Vis Spectrophotometer (Thermo Fisher Scientific). Experiments in day-night conditions were performed in a separate day-night chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Bioengineering 2024Quote: ... under the proliferative condition at 33°C with 10 units/mL mouse interferon gamma supplemented in fresh in RPMI 1640 basal media containing HEPES and GlutaMAX (Gibco, #72400120) with 10% FBS and 1% pen-strep ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Genomics 2020Quote: ... as instructed using antibodies against HA-tag (Thermo Fisher cat#26183), MYC-tag (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse monoclonal anti-HA Tag (ThermoFisher Scientific 26183, 1:10,000), used with either IRDve® 680LT anti-mouse (LI-COR ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was incubated with GST-TAG antibody (1:1000, ThermoFisher) or anti-MC4 (rabbit ...
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bioRxiv - Cell Biology 2021Quote: ... the grids were incubated with anti-HA tag antibody (Invitrogen, 5B1D10), 10X diluted in 1% BSA in PBS for 3 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Phosphopeptides were labeled with Tandem Mass Tag (TMT) (Thermo Fisher Scientific), desalted using SDB-XC StageTips ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Dried Tandem Mass Tag (TMT) 10-plex reagent (Thermo Fisher Scientific) was reconstituted at 20 µg/µl in 100% anhydrous MeCN and added to samples at a 1:1 TMT to peptide mass ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... Tandem Mass Tag (TMT)11plex Isobaric Label Reagent Set (ThermoFisher Scientific) was utilized for this application ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana was prebound to Strep-tag®II beads (Thermo Scientific) for two hours under rotation at 4 °C ...