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Citations for Thermo Fisher :
1451 - 1500 of 6394 citations for GITR Human T45A HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... 70% confluent HEK-293T cells were cultured for 24 h w/o antibiotics and transfected (Lipofectamine, Thermo Fisher Scientific) with lentiviral transfection constructs and packaging plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Neuroscience 2023Quote: The plasmid for heterologous expression of fshr-1 in HEK cells was obtained by directionally cloning the fshr-1 cDNA into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). The cDNA sequence of the fshr-1a gene isoform was amplified by PCR using cDNA from mix-staged wild-type C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and HEK 293T AAVS1 LP (HEK293T-LP, kind gift from D.M. Fowler) were cultured respectively in RPMI medium 1640 (Gibco) or DMEM medium (Gibco ...
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bioRxiv - Immunology 2022Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: Human SH-SY5Y neural cells and HEK-293T cells were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... 5×104 HEK 293T and 2.5×104 MDCK-SIAT1 cells were plated in 12 well plates in growth media (DMEM, Invitrogen #19965-092 ...
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bioRxiv - Immunology 2023Quote: ... HEK 293T cells and HeLa cells were transfected with plasmids using lipofectamine 3000 according to the manufacturer’s instruction (Invitrogen).
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bioRxiv - Immunology 2023Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clonal genes were delivered as purified plasmid DNA ready for transient transfection in HEK Expi293 cells (Thermo Fisher Scientific). Cultures in a volume of 1.2 mL were grown to 4 days ...
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bioRxiv - Bioengineering 2023Quote: All cell culture experiments were performed with HEK-293T cells (ATCC, CRL-3216) grown in 10% FBS / 90% Dulbecco’s Modified Eagle Medium (DMEM; Gibco) in a 37°C / 5% CO2 incubator with 100% relative humidity ...
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Synthetic Biology 2023Quote: HEK 293T cells were lysed 16 hours post-transfection with 200 µL of M-PER lysis buffer (Thermo Scientific) plus protease inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... and HEK 293T-hACE2 (BEI Resources, NIAID, NIH, NR-52511) were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×105 HEK 293T cells were plated on each respective well of a 4 well chamber slide (Thermofisher Scientific) and incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Genomics 2024Quote: 1.5 x 107 HEK 293T cells were transfected in a 15 cm tissue culture dish using Lipofectamine 2000 (Invitrogen) with 14.1 μg Brunello_iT7 plasmid library ...
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bioRxiv - Neuroscience 2024Quote: ... HEK 293T cells reaching 40-60% confluency on 35mm culture plates were transiently transfected using Lipofectamine 2000 (Thermo Fisher) 36-48 hours before experiments ...
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bioRxiv - Microbiology 2024Quote: ... Z-23GUINEA-18 and Z-4GUINEA-18 fragments were transfected in HEK-293T cells using Lipofectamine 3,000 (ThermoFisher, France). After 4 days ...
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bioRxiv - Genetics 2023Quote: Lentiviral plasmids encoding pCMV-OMP25-tagBFP2-EF1a-NEO and pUbC-UCR-2.3- mNeonGreen-EF1a-Puro were transfected into HEK-293T cells with lentiviral packaging plasmids with Lipofectamine 3000 (Invitrogen) according to standard lentiviral production protocols ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human Embryonic Kidney (HEK)293T cells (ATCC) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Bodinco and Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: GAL4–PAX6 was cloned into the pcDNA5/FRT/TO expression vector by standard procedures and subsequently used for recombination into the genomic DNA of TREx 293 Flp-In cells (Invitrogen) as described in details previously41 ...
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bioRxiv - Molecular Biology 2019Quote: Human embryonic kidney 293 T (HEK293T) cells were maintained in MEM/EBSS (HyClone) supplemented with 10% fetal bovine serum (FBS, Gibco), 100U/ml penicillin and 100μg/ml streptomycin (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified cosmid DNA (2 μg) was digested with Pac1 and transfected into 293 cells with Lipofectamine 2000 (ThermoFisher 11668019). Adenovirus plaques appeared 7 days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: All ectodomains were produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m. ...
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bioRxiv - Microbiology 2021Quote: ... whilst the growth medium of T-REx-293- derived cell lines stably transfected with pcDNA4/TO-based plasmids was further supplemented with zeocin (100 µg/mL, Gibco).
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bioRxiv - Biochemistry 2020Quote: HEK293F cells were seeded in 24 well plates (1 million cells in each well) in a final volume of 1 mL of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 ug DNA as described before and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... these cells were adapted to suspension culture by growing the cells in a mix of FreeStyle(tm) 293 Expression medium (Gibco) and DMEM/FCS and decreasing the concentration of DMEM/FCS at each passage ...
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bioRxiv - Immunology 2021Quote: ... 50μg of the plasmid was then transfected into Expi293F cells at a density of 3×106 cells/mL in a 250mL shaking flask using the ExpiFectamine 293 Transfection Kit (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... n372361, n372362) or Negative Controls (4390843, 4390846) and transfected into 293 cell lines using Lipofectamine RNAiMAX Transfection Reagent (13778030, ThermoFisher). Transfections were performed in a 24-well plate with 5 pmol of siRNA and 1.5 μl RNAiMAX Transfection reagent per well ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells stably expressing FLAG/HA-tagged human PRPF4 was generated using the pcDNA5/FRT-PRPF4 plasmid and Flp-In-293 cells according to the manufacturer’s protocol (Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Flp-In-293 cells were transfected with a 9:1 ratio of pOG44:pcDNA5/FRT-PRPF4 plasmids using Lipofectamine 2000 (Invitrogen) and 48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was expressed in FreeStyle 293-F cells (Invitrogen). The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells were seeded in 24-well plates (1 million cells in each well) in a final volume of 1 ml of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 µg DNA of different hSARM1 constructs and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... mAbs were produced by transient transfection into Expi293F™ cells with equal amounts of paired heavy- and light-chain expression vectors using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (Thermo fisher). Five days post antibody expression ...
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bioRxiv - Biochemistry 2022Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). Plasmids were transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Microbiology 2021Quote: HEK293T-ACE2 cells were reverse-transfected with plasmids and TransIT-293 at a ratio of 1 µg DNA : 3 µLTransit-T293 in Opti-MEM (Gibco). For an experiment conducted in triplicate at a 48-well scale ...
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bioRxiv - Molecular Biology 2020Quote: ... and pcDNA5/FRT/TO-based plasmids carrying the GFP-RIF1-L or GFP-RIF1-L-pp1bs gene were mixed in 9:1 molar ratio and used for transfection of Flp-In T-Rex 293 cells (Invitrogen) with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells grown to a density of 2.5 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for two days at which point the supernatant was harvested ...
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bioRxiv - Biochemistry 2020Quote: A 100 ml culture (1 × 106 cells/ml) of 293F cells were transfected with 100 µg of pCMV-FLAG-His6-NPC1L1-MLD plasmid using 293-fectin (Invitrogen) according to the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: NiV prefusion glycoprotein and avi-tagged probes were expressed by transfection in 293 Freestyle (293F) cells (Thermo Fisher Scientific, MA) using Turbo293 transfection reagent (SPEED BioSystem ...
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bioRxiv - Immunology 2020Quote: ... YP_009724390.1) ectodomain was produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m ...