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Citations for Thermo Fisher :
1401 - 1450 of 6394 citations for GITR Human T45A HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Overnight culture medium was removed and cells were then serum starved in 40 μl of Freestyle 293 media (Invitrogen) per well for 4 hours at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... P2 viruses (8%) were used to infect tsA201 cells grown in Freestyle 293 Expression Medium in suspension culture (ThermoFisher). Infected cells were incubated for an initial 12 h at 37 °C before 10 mM sodium butyrate was added ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293F cells were cultured in serum-free Freestyle 293 Expression media using 125 mL disposable culture flasks (Thermo Fisher). Cells were shaken constantly at 125 rpm at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The two plasmids were transiently co-transfected into HEK293F cells in FreeStyle™ 293 Expression Medium (ThermoFisher Scientific, UK). The cells were cultured in suspension phase at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting vector was transiently transfected into HEK293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific # A14525). After enhancement ...
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bioRxiv - Cancer Biology 2020Quote: ... human embryonic kidney cell line 293 and 293FT were cultured in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 10% FBS (Life Technologies), penicillin/streptomycin (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... by transfecting 200×106 of these cells with purified DNA using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Supernatants from transfected cells were harvested 3 days post transfection by centrifugation at 300g for 10 min and filtration through a 22um filter ...
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bioRxiv - Genomics 2021Quote: HEK-293 cells (ATCC CRL-1573) were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% FBS (Omega Scientific ...
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bioRxiv - Immunology 2020Quote: ... This plasmid was used to transiently transfect freestyle 293-F cells using the FreeStyle MAX reagent (Thermo Fisher Scientific). The spike ectodomain was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2022Quote: ... cells were grown in suspension to 1×106 cells/mL with serum-free FreeStyle 293 expression medium (Thermo Fisher), supplemented with 25 µg/ml penicillin/streptomycin ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney line 293 (HEK293) wildtype and HTT-null cells were grown in Dulbecco’s modified Eagle’s medium (Thermofisher #41965) supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher #10500064) ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Immunology 2023Quote: ... four expression vectors were transiently transfected into Expi293 cells using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (ThermoFisher). Antibody containing cell culture supernatants were harvested after 5 days of cultivation in shake flasks ...
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bioRxiv - Biochemistry 2022Quote: ... Glycoproteins were produced by transient transfection of exponentially growing Freestyle 293-F suspension cells (Thermo Fisher Scientific, Waltham, MA) using polyethylenimine (PEI ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Immunology 2024Quote: FreeStyle™ Expi293F™ cells were cultured in FreeStyle™ 293 expression medium according to the manufacturer’s instructions (Invitrogen). Additional cell lines were obtained from the American Type Culture Collection (ATCC) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: pcDNA4TO-TS-SNAP-β2AR or pcDNA4TO-TS-SNAP-β2AR-nLuc were stably transfected into T-RexTM-293 cells (Invitrogen) using polyethylenimine (PEI) ...
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bioRxiv - Molecular Biology 2020Quote: ... Human transferrin-568 (ThermoFisher) was added at 10μg mL−1 concentration in serum free media and incubated at 37°C to allow for internalization ...
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bioRxiv - Immunology 2021Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: Probes Hs00171064_m1 (human, ThermoFisher), Mm00440280_g1 (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human hepatocytes (Gibco) were seeded in the top channel at a density of 3.5 x 106 cells/mL using complete hepatocyte seeding media ...
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bioRxiv - Immunology 2020Quote: ... or anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 25 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... or human IgG (Invitrogen) as control ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (Gibco) at 50 μg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... human (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (ThermoFisher, #33016015); Laminin 111 ...
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bioRxiv - Immunology 2023Quote: ... A Human ProcartaPlexTM (Invitrogen) immunoassay was additionally used to detect 45 human cytokines ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... CD36 (Human tissue Thermofisher: PA1-16813 1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... Full length Cavin1 and ΔDR1 mutants were expressed and purified from suspension cells HEK-293F (Invitrogen, Carlsbad, CA, USA) as described previously (33) ...
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bioRxiv - Cancer Biology 2020Quote: HEK-293T cells were plated at a density of 1.0 × 106 cells/10 cm plate in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Biochemistry 2019Quote: Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose (Gibco)) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: HEK cells were grown in 6 well plates and transfected with 4µg of each DNA with lipofectamine 2000 (Invitrogen) for 6hrs according to manufacturers instructions.
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bioRxiv - Bioengineering 2019Quote: HEK 293T cells (authenticated by Bio-Synthesis, Inc. and tested negative for mycoplasma using a kit from Molecular Probes) were plated at low density on poly-L-lysine coated coverslips (18 mm ...
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bioRxiv - Cancer Biology 2020Quote: RPE1-hTERT and HEK 293T cell lines were purchased from ATCC and cultured in DMEM (41966052; Thermo Fisher Scientific) containing 10% fetal bovine serum (10500064 ...
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bioRxiv - Neuroscience 2020Quote: HEK 293T cells were cultured at 37°C in 5% CO2 in DMEM+ GlutaMax (Thermo Fisher, cat. no. 10566016) with 10% fetal bovine serum (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... pcRV-Gagpol and pMD2G vectors into HEK-293T cells with lipofectamine 3000 according to manufacturer instructions (Thermo Fisher, LF300001). Target cells were infected with lentivirus supplemented with 5µg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... HEK cells were transfected using Lipofectamine 2000 according to different siRNA quantity in Opti-MEM medium (Thermo scientific, USA). The transfected cells were infected with either CHIKV strains PS or IS with MOI 0.1 at 24 hours post transfection (hpt) ...
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bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
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bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
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bioRxiv - Immunology 2022Quote: Proteins were expressed by transient transfection of HEK 293F cells (0.8-1.2 million cells/mL) maintained in Freestyle medium (Life Technologies). A 3:1 ratio of PEImax and expression plasmids (312.5 µg/L cells ...
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bioRxiv - Biochemistry 2022Quote: A polyclonal HEK cell line that stably expresses AE1 upon tetracycline induction was generated using the TRex system (Invitrogen). Cells were plated in a 96-well plate and incubated overnight with or without 2 μg/ml tetracycline at 37° C ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and Fyn-KD-HEK 293T) were carried out using Lipofectamine 3000 according to the manufacturer’s instruction (Life Technologies). Cells were checked for expression of plasmid of interest after 18 hours post transfection by fluorescence microscopy ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 × 105 HEK-293A cells were seeded in 12-wells plates and transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with 180 ng pcDNA3-DDX3-HA ...
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bioRxiv - Immunology 2021Quote: HEK 293T-hACE2 and Vero E6 cells (RRID:CVCL_0574) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) containing 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: Plasmids encoding full length SARS-CoV-2 S (GenBank ID: QHD43416.1) with one or more mutation sites were carried into HEK-293T cells using lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... HEK 293T cells (2.5 × 105 cells/well in 24 well plates) were grown in DMEM (Gibco Cat# 11995-065) with 10% FBS and 1X PSA (100 units/mL penicillin ...