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Citations for Thermo Fisher :
1651 - 1700 of 6394 citations for GITR Human T45A HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were grown to a density of 3 × 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific). Three to 5 days post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 G614 or its mutants or S2 together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... All fusion proteins were expressed in Expi-293F cells in 250-280 ml culture using the ExpiFectamine 293 Transfection Kit (Life Technologies) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... and human embryonic kidney 293 cells (HEK293) were purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Fisher Scientific, #MT10013CV) with 10% FBS at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: The Flp-In cell line expressing GFP-CENP-E2055-2608 was generated using HeLa T-REx Flp-In 293 cells according to the Flp-In system protocol (Thermo Fisher). GFP-CENP-E2055-2608 was cloned into pcDNA5 FRT/TO vector ...
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bioRxiv - Microbiology 2023Quote: ... All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific). Four days after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). At 7 days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a mass ratio of 2:1 (HC:LC) using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). After transfection ...
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bioRxiv - Microbiology 2023Quote: HEK293S GnTI-(ATCC CRL-3022) suspension cells used for expression of HIV Env proteins were maintained in FreeStyle 293 expression medium (Life Technologies), supplemented with 1% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... elegans BUB-1 TPR (1-189)::3xFLAG under a CMV promoter was transfected into Freestyle 293-F cells (Thermo Fisher Scientific). 48 hours later ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells were grown to a density of 3 x 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and expression carried out for 3 to 5 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... A mixture of 15 μg of pcDNA3.4-V-gene-mIgG1 vector and 15 μg of pcDNA3.4-V-gene-kappa vector were transfected into Expi293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... a kind gift from Jason McLellan) was expressed by transiently transfecting transiently transfecting plasmid using FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Bioengineering 2024Quote: ... cells were diluted to 3 × 106 cells/mL in antibiotic-free Expi293 Expression Medium and transfected using an ExpiFectamine 293 Transfection Kit (Thermo Scientific). ExpiFectamine 293 Transfection Enhancers 1 and 2 were added 20 h post transfection and cultures were incubated for 5 days post-transfection ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Transfer and packaging vectors were transfected into HEK 293T cells to produce lentiviruses using Lipofectamine 3000 reagent (ThermoFisher Scientific, MA, USA). Lentiviruses were harvested 48hr post transfection ...
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bioRxiv - Immunology 2022Quote: ... In-house expressed antibodies were made through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: HEK-293T cells (293T/17, ATCC) were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS, Life Technologies). U2OS cells (ATCC ...
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bioRxiv - Genomics 2021Quote: ... Human embryonic kidney (HEK) 293T cells (CRL-11268, ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (11965-092, Gibco, Gaithersburg, MD, USA) supplemented with 10% (16000044 ...
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bioRxiv - Cell Biology 2021Quote: ... and pCMV-dR8.91 (Zufferey et al., 1997) (5 mg each) were transfected into HEK 293T/17 cells using Lipofectamine LTX (Thermo Fisher Scientific). Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: A lentiviral pLKO.1-puro constructs targeting KSR1 and non-targeting control were transfected into HEK-293T cells using trans-lentiviral packaging system (ThermoFisher Scientific). The virus was collected ...
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bioRxiv - Cancer Biology 2022Quote: ... MSCV KSR2 IRES GFP resistant to binding hairpin sh5 (sh5RKSR2) was transfected into HEK-293T cells using trans-lentiviral packing system (ThermoFisher Scientific). The virus was collected 48 hours post transfection and used to infect KP1 sh5 cells with 8 ug/mL Polybrene for 72 hours ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: 106 HEK cells were plated in 6-well plates and transfected with the indicated plasmids with Lipofectamine 2000™ reagent (Invitrogen). 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Flag-CTPS1H355 and TRE-promoter driven myc-IMPDH2 and myc-IMPDH2Y12A were delivered into HEK 293T or HeLa cells with TurboFect™ transfection reagent (R0532, ThermoFisher). The expression of myc-IMPDH2 was turn off with culture medium containing 1 μg/ml doxycycline (ab141091 ...
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bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells (INS-1 832/13 and HEK-293T) were seeded onto poly-D-lysine coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a cell density of 70,000 cells/cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... Expression characteristics of each plasmid were evaluated in HEK 293T cells by transient transfection using Lipofectamine™ 3000 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... HepG2 and HEK 293T cell lines were cultured in Dulbecco’s modified eagle medium (DMEM high glucose, Life Technologies, Carlsbad, CA, USA), supplemented with 10 % (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transiently transfected in HEK-293T cells using polyethyleneimine (PEI, PolyAR) in a 1:2.5 DNA:PEI ratio in OptiMem medium (Thermo Fisher Scientific, 22600050). The supernatant containing recombinant proteins was harvested 72 h post-transfection and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, United States) supplemented with 10% FBS (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... commercially synthesized RNA with m6A methylation (Sequence: GGAAAGGA(m6A)CUAAAAA) and the corresponding unmethylated RNA were transfected into HEK-293T cells by lipofectamine 2000 (Invitrogen, 11668019), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T cells for viral vectors production were cultured in DMEM high-glucose (w/ 4.5 g/l; #11995065; Thermo Fisher Scientific) with 10% heat inactivated FBS ...
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bioRxiv - Physiology 2023Quote: CHO-Kv7.2/7.3 and HEK-CaSR were cultured separately in Gibco™ Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 (Life Technologies) supplemented with 10% foetal bovine serum (Life Technologies) ...
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bioRxiv - Genomics 2023Quote: ... Two 15-cm dishes were each seeded with 1.5×107 HEK 293T cells (ATCC, CRL-3216) in 25 ml DMEM complete medium: DMEM (Gibco, 11965-092) supplemented with 10% FBS (VWR ...
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bioRxiv - Cancer Biology 2023Quote: Metastatic breast cancer cell line MDA-MB-231 and HEK-293T cells were maintained in DMEM/F12 medium (Gibco, ThermoFisher Scientific), supplemented with 10% FSB and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: Metastatic breast cancer cell line MDA-MB-231 and HEK-293T cells were maintained in DMEM/F12 medium (Gibco, ThermoFisher Scientific), supplemented with 10% FSB and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... 293 and HEK 293T cells were cultured at 37°C with 5% CO2 in Dulbecco’s modified Eagle medium (DMEM) (Gibco, Carlsbad, USA) containing 2 mmol/L L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T/17 cells were maintained at 30° C in a low-glucose formulation of DMEM (Cat # 12320–032; ThermoFisher Scientific), as previously described (22).
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bioRxiv - Cell Biology 2024Quote: ... The pLV vectors were co-transfected with packaging plasmids (Celecta #CPCP-K2A) into HEK 293T cells using Lipofectamine 3000 reagent (Invitrogen #L3000001) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2024Quote: HEK 293T cells (UCSF Cell Culture Facility) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco #10569-010) and 10% fetal bovine serum (FBS ...