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Citations for Thermo Fisher :
1701 - 1750 of 6394 citations for GITR Human T45A HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Cells were cultured in RPMI-1640-Glutamax (H358) or DMEM (A549, HeLa, HEK-293T) medium (GibcoBRL, Life Technologies, Cergy Pontoise, France) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral packaging plasmids (pCMV-D8.9 and pCMV-VSVG) and reporter plasmids were transfected into HEK-293T cells using Lipofectamine 3000 (ThermoFisher cat#L3000001). Virus-containing supernatant was harvested at 48 and 72 hours after transfection and viral particles were concentrated with Lenti-X Concentrator according to the manufacturer’s instructions (Takara Bio cat#631232) ...
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bioRxiv - Neuroscience 2024Quote: Protein lysates from HEK cells or two-month-old mice frontal cortex were prepared using Pierce IP Lysis Buffer (Thermo Scientific) supplemented with cOmplete EDTA-free Protease Inhibitor Cocktail (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: Luciferase emissions from transfected Hek-Luc cells were measured using the Pierce® Firefly Luciferase Glow Assay Kit (Thermo Fisher Scientific). To initiate cell lysis ...
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bioRxiv - Neuroscience 2024Quote: ... Both HEK cells and astrocytes were transfected the day after plating with 500 ng of plasmid DNA using lipofectamine 2000 (Invitrogen #11668019) and OptiMEM (Life Technologies #31985-070) ...
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bioRxiv - Cell Biology 2024Quote: Human FLAG-ATGL (Uniprot ID: Q96AD5) was expressed in 20 15-cm dishes of HEK 293T cells were cultured in DMEM (ThermoFisher Scientific) with 5% FBS and then transiently transfected with a solution of 1 µg mL-1 plasmid DNA and 1 mg mL−1 PEI ...
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bioRxiv - Cancer Biology 2024Quote: ... lentiviral vector DNA was transfected with psPAX2 and pMD2.G packaging plasmids into HEK-293FT cells using Lipofectamine 2000 transfection reagent (Invitrogen, 11668019) and D10 media lacking antibiotics ...
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bioRxiv - Microbiology 2024Quote: HEK 293T cells sorted as described in ‘Flow Cytometry’ below were counted using the Countess II Cell Counter (Thermo Fisher Scientific) and cell samples were diluted such that all conditions contained the same cell numbers as input ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant constructs of heavy and light chain were transfected at 1:1 ratio into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (Gibco™ ...
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bioRxiv - Immunology 2021Quote: ... The HA–6XHIS and HA–AviTag constructs for each HA were co– transfected using 293Fectin Transfection Reagent into FreeStyle™ 293–F Cells (ThermoFisher Scientific) at a 2:1 ratio ...
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer” s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant antibodies were expressed in Expi293 cells following co-transfection of heavy and light chain plasmids (1:1 ratio) using Expifectamine 293 (Thermo Fisher Scientific). Supernatants were harvested after 5-6 days ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Munc13L (with or without Halo-tag) was expressed in ExpiHEK-293 cell cultures using expifectamine as a transfection reagent (Thermofisher, Waltham, MA). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... Flp-In T-REx 293 cells stably expressing AKAP95 WT or mutants were generated using the Flp-In system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were selected for FRT site-specific recombination into the genome and stable Flp-In 293 T-REx cell lines were maintained with hygromycin (Life Technologies, 10687010) at 2ug/ml ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells growing at 37°C in 5% CO2 atmosphere in shake flasks in FreeStyle 293 medium were transfected with the corresponding plasmids using ExpiFectamine reagent (Thermo Fisher Scientific). Conditioned medium containing secreted proteins was harvested twice ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Expi293F cells were transfected at a density of 2 × 106 /ml with 400 ng (pCEP4-myc-ACE2 plasmids) or 500 ng (pCEP4-myc-S plasmids) per ml of culture using ExpiFectamine 293 (Thermo Fisher Scientific) according to the manufacturer’s directions ...
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bioRxiv - Biophysics 2020Quote: ... This plasmid was subsequently transfected with the pOG44 plasmid encoding the FLp recombinase into the Flp-In™ T-Rex 293 cell line (#R78007, Thermo Fisher Scientifi ...
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bioRxiv - Bioengineering 2021Quote: ... sequence coding for the amino acid 319-541 and fused with the N-terminal SARS-CoV-2 spike secretory signal and a C-terminal hexa-histidine tag was transfected into 293F cells cultivated in Freestyle 293 expression media (Thermo Fisher, #12338018) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... plasmids with antibody heavy and light chain genes were used to transfect suspension Expi 293i cells using ExpiFectamine 293 transfection reagents (Thermo Fisher Scientific) as described (20) ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Immunology 2022Quote: ... Cells grown to a density of 3 million per mL were transfected using the respective plasmids with the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) and cultivated for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid DNA (1µg/mL) and ExpiFectamineTM 293 reagent were individually diluted with Opti-MEM Reduced Serum Medium (Thermo Fisher, Waltham, MA), incubated 5 min at room temperature (RT) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells in a total culture volume of 10 ml were transfected with 10 µg of plasmid DNA using the ExpiFectamineTM 293 Transfection Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Individual plasmids or combinations of (sub)species-specific gH and gL expression plasmids were transfected into FreeStyle 293-F cells (Thermo Fisher Scientific) using polyethylenimine (PolySciences) ...
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bioRxiv - Biophysics 2023Quote: S1PR1-Flag and CD69-StrepII were separately expressed using baculovirus-mediated transduction of mammalian HEK293S GnTI− cells (ATCC CRL-3022) in a medium containing FreeStyle 293 (Gibco Cat# 12338018) supplemented with 2% charcoal-dextran stripped fetal bovine serum (Gibco Cat# 12676029) ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were produced by transient co-transfection of Expi293F cells (maintained in serum-free Expi293 Expression Medium) with heavy- and light-chain constructs using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific), and subsequently purified using Protein G Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... After verification by sequencing the constructs were subsequently introduced into Flp-In™ T-REx™ 293 cells (Life Technologies, Carlsbad, CA) as described by Liu et al 52,53.
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested three days post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK-293 cells with 80% of confluency were transfected using 250ng/ml of pCMVHA hEZH2 and Lipofectamine 2000 (#11668030, Invitrogen, Thermo Fisher Scientific) for 4 hrs ...
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bioRxiv - Neuroscience 2023Quote: ... was expressed in Expi293 cell line (3X106 cells/ml, 200 ml in 1 L flask) using ExpiFectamine™ 293 Transfection Kit (Gibco, A14524). After 48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... The ectodomain of ancestral SARS-CoV-2 spike (GenBank: MN908947.3) was expressed as previously described.10 The protein was purified from FreeStyle 293-F cells (Thermo Fisher Scientific, USA) using affinity chromatography followed by size exclusion chromatography ...
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bioRxiv - Microbiology 2023Quote: ... Expi293F cells were diluted to a density of 3 million cells per mL and transfected using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cells were incubated shaking at 130 rpm at 37°C and 8% CO2 ...
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bioRxiv - Biophysics 2023Quote: ... 1 L of Expi293 cells were grown at 37 °C under a 5% CO2 atmosphere in Gibco® FreeStyleTM 293 Expression Medium (ThermoFisher Scientific) to reach the density of 2 × 106 mL-1 ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested four days post-transfection and proteins were purified using HisTrap™ High Performance column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... SJ293TS cells were transfected with the transfer vector and the helper plasmids pCAG-kGP1-1R, pCAG-VSVG and pCAG4-RTR2 using PEIpro (Polyplus Transfection, Strasbourg, France) and grown in Freestyle 293 Expression media (Thermo Fisher Scientific) at 37°C with 8% CO2 and shaking at 125 RPM ...
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were transiently expressed in FreeStyle 293-F cells using pSecTag2A plasmids and 293Fectin transfection reagent according to the suppliers’s protocol (Thermo Fisher Scientific; 12347019). 4-6 days after transfection ...
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bioRxiv - Immunology 2023Quote: ... The HA–6XHIS and HA–AviTag constructs for each HA were co–transfected using 293Fectin Transfection Reagent into FreeStyle™ 293–F Cells (ThermoFisher Scientific) at a 2:1 ratio ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested four days post-transfection and proteins were purified using HisTrap™ High Performance column (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... comprising the variable heavy chain and light chains joined with the 15-residue linker was transiently expressed in a secreted form using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1x MEM non-essential amino acids (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: A mixture of 1 μg of antibody-expressing plasmid was transfected into FreeStyle 293 cells using the 293fectin Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions and cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) and cultured at a density of 3-5×106 cells/ml in a 37°C incubator with ≥80% relative humidity and 8% CO2 on an orbital shaker at 125×g for 3-4 days ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...