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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Chondrocytes were collected and cultured in an incubator containing 5% CO2 at 37°C with Dulbecco’s DMEM/F12 (HyClone) supplemented with 10% fetal bovine serum (FBS, Gibco) and 100 U/ml penicillin G sodium ...
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bioRxiv - Cell Biology 2023Quote: ... the tubules were digested using 10 ml of DMEM-F12 buffer containing 5 mg of trypsin (Thermo Fisher 27250018) on a 50 ml conical tube and the reaction was carried out on a 35°C shaker water- bath ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBS/5% FBS at 37°C for 10 min or 2 μM H2-DCFDA (Thermo Fisher Scientific, C6827) in PBS/5% FBS at 37°C for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae cells (OD 5-10) were pelleted and lysed in 200 µL 0.9% physiologic salt supplied with 2 µL RNAse cocktail (Invitrogen) and 5mg/mL Zymolyase 100T (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2023Quote: ... Dispase 5 mg/mL [GIBCO], Y-27632 10.5 μM [Sigma], DNase I 10 μg/mL [Sigma], Advanced DMEM/F12 [GIBCO] ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 8-10 times in PBS for 5 minutes and incubated with DAPI (0.1 mg/ml in PBS; #62248, Invitrogen) to stain nuclei ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 1:10 for quantitative real-time PCR (qPCR) run on an Applied Biosystems QuantStudio™ 6 fast real-time PCR system (Applied Biosystems, Foster City, CA) using PowerUp SYBR green master mix (Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... the transwell was moved to a standard 6-well cell culture plate and submerged in 10% neutral-buffered formalin (Fisher Scientific Cat. #22-026-435) for at least 24h ...
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bioRxiv - Cancer Biology 2019Quote: ... 300,000/6-well) and IDC (5,000/96-well; 100,000/6-well) cells were seeded in regular (Thermo Fisher Scientific) or ULA (Corning Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were forward transfected in 6-well dishes by using 6 μl of FuGENE® HD (PRE2311, Fisher Scientific) with 3 μg of sgRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2020Quote: Cells in the 6-well plates were transfected with 2μg plasmid of AMPKα or OGG1 via 6 μl Lipofectamine 2000 (Invitrogen) for 6 hours following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The Cas9-sgRNA-containing plasmid and donor vector were co-transfected at a molar ratio of 1:6 into XX mESCs cells on a 6-well plate using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher). Transfected cells were passaged at different densities into three Petri dishes with feeders ...
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bioRxiv - Biochemistry 2021Quote: ... 6% native PAGE gels were prepared by first making a gel solution containing 6% acrylamide (29:1) (Fisher Scientific).15 This solution was then degassed under vacuum and mixing ...
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bioRxiv - Developmental Biology 2020Quote: Skin tissues collected from newborn Foxn1−/− (Cby.Cg-Foxn1
/cmdb) and Foxn1+/+ (C57BL/6; B6) mice were incubated in dispase (6 U/ml; Life Technologies) overnight at 4°C ... -
bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
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bioRxiv - Molecular Biology 2020Quote: ... 2:6 and 3:6 dilution ratios to allow efficient selection of Hygromycin B (Thermo Fisher Scientific Catalog Number: 10687010). The Hygromycin selection was started at the 48 hours after transfection time point with a final concentration of 150µg/ml and refreshed every 3-4 days until the control non-transfected cells on a separate plate were completely dead (takes approximately 3 weeks from the start of transfection until the cells are expanded and frozen) ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated without ligand or with EGF (6 μM) or EREG (6 μM) in buffer A containing disuccinimidyl suberate (DSS) at 62.5 μM (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: 293T-ACE2 and HT1080-ACE2 cells were seeded in 24-well plates and 6-well plates respectively to achieve 70% confluency after 4-6 hours and then transfected with Lipofectamine RNAiMAX (Thermofisher) using the indicated dsiRNAs (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transiently transfected in 6-well plates with 6 individual IFNLR1-gRNA plasmids using Lipofectamine 3000 (Life Technologies). The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... treated wild-type C57BL/6 and 12-week-old Rad1 CKO mice were subjected to phosphopeptide enrichment and 6-plex TMT (ThermoFisher) labeling (Sims et al. ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was transfected into the Sf9 insect cells seeded in 6-well plates at 0.5 × 106 cells/ml using Fugene 6 reagent (Invitrogen protocol). At 72 hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Cancer Biology 2021Quote: ... COS-1 cells were transiently transfected with either 5 or 10 μg of each of pCIneo-FOXA1-V5 and pCIneo-V5 plasmids using lipofectamine 3000 Reagent (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... and 20 µg protein lysate was denatured at 95°C for 5 min and then separated on a 10-20% Tris-tricine gel (Invitrogen) at 125V for 90 min ...
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bioRxiv - Cell Biology 2020Quote: ... sections / cells were then incubated in PBS−/− containing either DAPI (5 μg ml−1; 10 min; RT; Thermo Fisher Scientific) or DRAQ5 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings were made with patch pipettes (3-5 MΩ) containing aCSF and 10 µM alexa-594 (Thermofisher, Waltham, MA, USA) and CSFcNs were targeted under visual guidance using their fluorescence ...
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bioRxiv - Molecular Biology 2021Quote: ... acetonitrile/water (95/5) with 10 mM ammonium acetate (pH 8.2) (All solvents were LC–MS Optima grade from Fisher Scientific). The total run time was 11 min ...
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bioRxiv - Molecular Biology 2021Quote: HEK-293 cells used in the endogenous UPF1LL knockdown and RNA-seq experiments were received from ATCC (CRL-3216) and maintained at 37°C and 5% CO2 in DMEM with 10% FBS (Gibco) and 1% pen/strep ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T cells were grown at 37 °C and 5 % CO2 in DMEM supplemented with 10% tetracycline-free fetal bovine serum and 1% Penicillin-Streptomycin (Invitrogen). Cells were transfected with the LentiX VSV-G transfection mix (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Molecular Biology 2021Quote: ... For RNA-extraction: Three replicates of 10 (Oil and Nic) or 5 (Phe) pair of eyes were either added to RNAlater (Invitrogen) (Oil ...
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bioRxiv - Biophysics 2022Quote: ... the cells were resuspended at 5-10×106 cells per ml in HBSS without Ca2+ and Mg2+ (Thermo Fisher Scientific) to ensure their quiescence prior to stimulation by contact with IgG.
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bioRxiv - Developmental Biology 2019Quote: The E11.5 Yolk sacs from miR144/451+/GFP mouse embryos were first collected in 1xPBS+10% Fetal Bovine Serum (10270106, Gibco). Upon dissection they were fixed in 4% Paraformaldehyde solution in PBS (sc-281692 ...
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bioRxiv - Cell Biology 2019Quote: ... Porcine jejunum epithelial cell line IPEC-J2 (ACC-701) was grown in DMEM F12 medium supplemented with 10% FBS and 5% penicillin/streptomycin (P/S, Gibco) in 75 cm2 culture flasks ...
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bioRxiv - Neuroscience 2019Quote: ... The cultures were recovered in serum-containing media (Neurobasal media supplemented with 10% horse serum, 5% fetal bovine serum, and 2 mM glutamine [Gibco]) for 4 h and maintained in serum-free media (Neurobasal media supplemented with B-27 (1:50 diluted) ...
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bioRxiv - Cancer Biology 2020Quote: ... maintained in Dulbecco’s modified Eagle’s Medium (DMEM) with 10% FBS at 37°C and 5% CO2) were transiently transfected using Lipofectamine 2000 (Life Technologies). Retroviral particles were obtained and concentrated by centrifugation of the conditioned media (6000 × g overnight at +4°C) ...
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bioRxiv - Systems Biology 2020Quote: ... sonicated for 5 min and infused at 10 µL/min into a Thermo Q Exactive Plus spectrometer (Thermo Fisher Scientific) equipped with the HESI II ion source for shotgun lipidomics ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells and HeLa cells were cultured at 37°C in 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco).
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bioRxiv - Biochemistry 2020Quote: ... was added to the cells and incubated in the dark for 5-10 minutes prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done in duplicate and relative luciferase units (RLU ...
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bioRxiv - Molecular Biology 2021Quote: ... aliquots of 10 x 104 uninfected macrophages suspended in 10 mL SFB-free DMEM were incubated with: i) 5 µg of lipofection pBlueBacHis2/CAT plasmid (Invitrogen); ii ...