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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: HEK293T cells were cultured at 37°C in air containing 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (Gibco) and penicillin (100 unit/mL)-streptomycin (0.1 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Passages were made before reaching confluence every 2-3 days by two washes with PBS (15 min each) and incubation in trypsin 0.05% for 5-10 min (Trypsin Cat#15400054, Thermo Fisher). MDCK cells stable transfected clones (MDCK-Shh ...
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bioRxiv - Microbiology 2020Quote: Purified RNA of 10 μg was denatured at 95°C for 5 min in gel loading buffer II (#AM8547, Invitrogen) and separated by 6% polyacrylamide gel with 7M urea at 300 V for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 65°C for 5 min and treated with 10 U RNase-free DNase I (ThermoFisher Scientific, Waltham, USA) in DNase 1 buffer at 37 °C for 40 min ...
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bioRxiv - Microbiology 2020Quote: ... Aag2 cells were grown at 30 °C with 5% CO2 in DMEM high glucose media supplemented with 10% heat-inactivated fetal bovine serum (Gibco), 1% penicillin-streptomycin ...
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bioRxiv - Neuroscience 2020Quote: Culture medium was replaced with Fluo-4 AM (5 μM) or X-Rhod-1 AM (10 μM) (both from Invitrogen) working solution in Ca2+ solution (pH 7.4) ...
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bioRxiv - Genomics 2020Quote: ... velifer embryonic fibroblasts into 6 well plates and transfected with the resulting Cas9-gRNA vector pair (5 μg DNA each, for a total of 10 μg DNA) via electroporation (ThermoFisher Neon Transfection System #MPK10025 ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Cancer Biology 2021Quote: For quantification of target expression, HPAECs (Day 0, Day 5, Day 10 of chip culture) were recovered using TrypLE Express Enzyme (Gibco) at 37°C for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Cell Biology 2020Quote: ... CD140a-PE (10 μl/106 cells, 558002, BDBioscience, Eysins, Switzerland) and CD140b-APC (5 μl/106 cells, A15719, Thermo Fisher). Right before analysis ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 was cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 5% Penstrep (Invitrogen and Himedia) in an incubator fixed at 37°C and equipped with 5% CO2 supply ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were grown at 5% CO2 and 37 °C in DMEM high glucose media supplemented with 10% heat inactivated fetal bovine serum (FBS) (Gibco), penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Microbiology 2022Quote: ... An equivalent of 0.25 midgut and 5 salivary glands were resolved in a NuPAGE™ 10% Bis-Tris Protein Gel (Invitrogen) under reducing conditions and transferred to a PVDF membrane Invitrogen™ Power Blotter Select Transfer Stacks ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were dried for 30 mins in a 37°C incubator and washed 3×5 min with 0.1% TritonX-100 in PBS (PBST) and incubated in 10% Horse Serum (ThermoFisher, #26050070), 0.4% TritonX-100 in 1x PBS (blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... at standard 5% CO2 and atmospheric O2 at 37°C in DMEM (5.5 mM glucose) supplemented with 10% FBS (Thermofisher #10437010), 50 μg/ml uridine (Sigma-Aldrich #U6381) ...
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bioRxiv - Cancer Biology 2022Quote: ... These three cell lines were grown at 5% CO2 and 37° C in RPMI 1640 without L-Glutamine media supplemented with heat inactivated 10% FBS (Gibco), penicillin/streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... The filtrate was spun at 500 x g for 5 min to pellet the cells that were then resuspended in Ham’s F-10 growth media (Thermo Fisher), 20% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and these cells were cultured by a standard protocol at 37 °C with 5 % CO2 in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (ThermoFisher).
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bioRxiv - Plant Biology 2021Quote: ... The 10 μL reaction mixture was prepared as follows: 5 μL TaqMan™ Fast Universal PCR Master Mix (Applied Biosystems), 0.2 μL each of forward and reverse primers (0.2 uM) ...
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bioRxiv - Cancer Biology 2020Quote: ... The CH12.F3 mouse B lymphocyte cell line (a generous gift from T. Honjo (Kyoto Univ.)) was cultured in RPMI/10% FCS/5% NCTC-109 (Invitrogen)/1X Penn-Strep/50μM αMeOH ...
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bioRxiv - Microbiology 2020Quote: Vero Ccl-81 cells (ATCC) were maintained Dulbecco Modified Eagle media supplemented with 5% Fetal Bovine Serum 10 units/mL penicillin (Gibco), 10 μg/mL streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: ... followed by buffer exchange using the 0.5 ml centrifugal filter described above (cat. no. UFC503024, MilliporeSigma) and 10 mM ammonium acetate buffer (cat. no. AM9070G, Invitrogen).
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... boiled for 5 min and size-fractionated by gel electrophoresis on NuPAGE 10% Bis-Tris 1.5-mm gels (Life Technologies) in 2-(N-morpholino ...
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bioRxiv - Physiology 2022Quote: ... The DMEM medium was supplemented with 5% penicillin/streptomycin resistance solution (Sangon, China) and 10% fetal bovine serum (Gibco, USA). The cells were grown at 37 °C with 5% CO2 and sub-cultured every 2–3 days ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown at 37°C in 5% CO2 on growth medium consisting of DMEM supplemented with 10% GlutaMAX (Thermofisher), ampicillin ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then permeabilized and blocked for 5 min in DPBS containing 0.2% saponin and 10% FBS (Thermo Fisher Scientific). Cells were stained with primary (1:25-50 ...
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bioRxiv - Bioengineering 2019Quote: ... or human organoid basal culture media consisting of conditioned media produced using stably transfected L cells (Wnt 50%; R-spondin 10%; Noggin 5%) and the following growth factors: B12 1X (Invitrogen), Nicotinamide 10mM (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... was added to the cells and incubated in the dark for 5-10 minutes prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done in duplicate and relative luciferase units (RLU ...
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bioRxiv - Cell Biology 2020Quote: ... The loading solution contained a 1:10 mixture of 5 mM Fluo-4 AM in dry DMSO and PowerloadTM concentrate (Invitrogen), which was diluted 100-fold into extracellular Tyrode’s solution (NaCl 140mM ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were cultured at 37°C and 5% CO2 in DMEM containing 10% [vol/vol] foetal bovine serum (Invitrogen). For passaging ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Developmental Biology 2021Quote: ... all cells were cultured at 37°C, 5% CO2 in growth medium (DMEM, 10% Embryonic Stem Cell qualified FBS (Gibco), NEAA ...
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bioRxiv - Cancer Biology 2021Quote: ... Suspension was centrifuged at 200g for 5 minute and cells were resuspended in 1ml of full medium (DMEM + 10% FBS; Gibco). Cells were either plated in 2D on cell culture treated flat bottom 96-well plates (Falcon ...
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bioRxiv - Neuroscience 2019Quote: ... with 10% fetal calf serum (FCS) at 37°C in an atmosphere with 5% CO2 and transfected with lipofectamine (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were washed in PBS (2×10 min at 1500 rpm) and RPMI 1640 + 10% fetal bovine serum (FBS) (5 min at 1500 rpm) (all products from ThermoFisher). Monocytes were isolated with anti-CD14 microbeads (Miltenyi ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... and mAb 18B7 (10 µg/ml) for 30 minutes followed by incubation with Alexa-Fluor 546 anti-mouse IgG1 (5 µg/ml; Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... 5% DTT) for 10 min at 70 °C before being loaded on a 4-12% Bis-tris gel (ThermoFisher NP0322BOX) at 10 µg/lane ...
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bioRxiv - Genomics 2021Quote: ... American Type Culture Collection (ATCC)] were cultured at 37°C and 5% CO2-concentration in DMEM supplemented with 10% fetal bovine serum (Gibco Invitrogen), 100 units of potassium penicillin and 100 μg of streptomycin sulfate per 1 ml (Lonza) ...
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bioRxiv - Biochemistry 2020Quote: ... charged by Ni2+ ions or 1 ml Tricorn 5/10 column (Cytiva) packed by Ni-NTA resin (Thermo Fisher Scientific). Columns were equilibrated by the 50 mM Na-PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... T-rex 293 cells (approximately 3 × 106 cells per 10-cm dish) were transfected with 5-HT2C wild-type or mutant DNA using Lipofectamine 2000 (Invitrogen) following manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of CD4-expressing cells was measured after incubation for 10 minutes with 1 µl of anti-CD4 antibody coupled to Alexa 647 (rat isotype, RM4-5, Invitrogen). For each cell line ...
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bioRxiv - Microbiology 2021Quote: ... or hydrogen peroxide (5 and 10 mM) and incubated with or without 50 μM of diphenyl-1-pyrenylphosphine (DPPP) (ThermoFisher) at 37°C (68 ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were dried for 20 min at room temperature then resuspended in 5 μL of 1 M Tris and 10 μL of protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Immunology 2021Quote: ... were added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher). Data was processed using GraphPad Prism v9.0.
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed in PBS (2×10 min at 1500 rpm) and RPMI 1640 + 10% fetal bovine serum (FBS) (5 min at 1500 rpm) (all products from ThermoFisher). Monocytes were isolated with anti-CD14 microbeads (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293T cells were cultured at 37°C in 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Biological Industries) ...