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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Cancer Biology 2023Quote: BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Invitrogen D3821) was used to assess the ability of 3T3s to transport fatty acids into the cell from their surroundings ...
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bioRxiv - Plant Biology 2019Quote: ... in hydrochloric acid 0.01 N (1:3, v:v) (Fisher Scientific; Hampton, New Hampshire, USA).
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bioRxiv - Biochemistry 2020Quote: Acetic Acid (Fisher Scientific, Cat. # 351269-4)
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bioRxiv - Biochemistry 2021Quote: Acetic Acid (Fisher Scientific, Cat. # 351269-4)
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bioRxiv - Plant Biology 2022Quote: ... RNAs are treated with Lucigen Baseline-ZERO DNase and purified by acid-Phenol-Chloroform pH:4.5 (Invitrogen™, Thermofisher). RT-PCR was performed using the SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Invitrogen™ ...
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bioRxiv - Plant Biology 2022Quote: ... RNAs are treated with Lucigen Baseline-ZERO DNase and purified by acid-Phenol-Chloroform pH:4.5 (Invitrogen™, Thermofisher). RT-PCR was performed using the SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Invitrogen™ ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, concentration 5 mg/mL, Invitrogen, cat. M6494) in PBS was added to each well containing culture medium and incubated for 2.5 h at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability was determined by the MTT [3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Invitrogen, Waltham, MA) reduction assay ...
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bioRxiv - Cancer Biology 2021Quote: ... conditioned media was replaced by a 1.2 mM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Synthetic Biology 2020Quote: ... and cell viability was then determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay (Invitrogen, USA) according to the manufacturer’s protocol 28 ...
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bioRxiv - Neuroscience 2023Quote: Cellular viability was determined utilizing a MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Invitrogen, Waltham MA), as described previously (Kumar et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (1:10,000, Invitrogen) for 3 min and eventually coverslips were mounted with Dako mounting kit (Fluka) ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI was used (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10 000, Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... and 1:500 dilution of DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (ThermoFisher). The brain slices were washed 3x with 1XPBS and then mounted with mounting media (Fluoro-Gel ...
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bioRxiv - Immunology 2020Quote: ... and stained for DAPI (4′,6-diamidino-2-phenylindole) (Life Technologies, 1:1000). For each condition ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were stained with 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, 1:10,000) in 1X PBS for 5 min.
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bioRxiv - Cell Biology 2024Quote: ... and stained with 4’,6-diamdino-2-phenylindole (DAPI) (1:500, ThermoFisher, 62248) in 1X DPBS for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... sections were counterstained using 4’,6-diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) and mounted with glycerol-gelatin aqueous slide mounting medium (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... to which 1 μl of 4′,6-diamidino-2-phenylindole (DAPI) (D1306, Invitrogen) was added ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded 4–5 × 104 cells per well in 8-well Lab-Tek II chambered cover glass slides (Nunc, Rochester, NY, USA) and incubated overnight ...
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bioRxiv - Microbiology 2024Quote: Proteins were subjected to reduction in 4X reducing sample buffer (8% SDS, 400 mM Dithiothreitol) and resolved on a precast 4%–12% SDS-polyacrylamide gel (Thermo Fisher Scientific, #XP04125BOX). Coomassie staining was performed using Imperial Protein Stain (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Microbiology 2021Quote: Fixed cells wells were resuspended in PBS containing 8 μg ml−1 Alexa Fluor 555 NHS ester (Invitrogen) and incubated at room temperature for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... The crosslinking reaction was stopped with 1/10 volume of 1.25 M glycine pH 8 (Thermo Fisher Scientific). Crosslinked cell pellets were washed 3 times with ice-cold DPBS followed by lysis or snap freezing and storage at −80°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... at a 1:8 dilution with an ABI PRISM 3130 Avant Genetic Analyser automated sequencer (Applied Biosystems, USA) and the primers included in the PCR protocols.
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bioRxiv - Developmental Biology 2020Quote: ... 1 × 107 HTR-8/SVneo cells were homogenized using RIP lysis buffer plus RNase inhibitor (Thermo Fisher Scientific) and a proteinase inhibitor cocktail (Roche) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were 1 x washed and incubated with 8 µg / ml Alexa488 labelled anti Mouse IgG (ThermoFisher, A11001) for 1 hr at RT in the dark ...
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bioRxiv - Genomics 2021Quote: ... cells were resuspended in 1 mL cold lysis buffer: 10 mM Tris-HCl pH 8 (Thermo Scientific, AM9855G), 10 mM NaCl (Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... was coated with Oregon Green 488-conjugated gelatin (Invitrogen; diluted 1:8 in solution of 2.5% (w/w) gelatin/2.5% (w/w ...
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bioRxiv - Molecular Biology 2022Quote: ... the pH was lowered to 8 by addition of 1 M Tris base and monobromobimane (mBBr, Life technologies) was added in excess to derivatize the CoASH ...
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bioRxiv - Physiology 2024Quote: ... This valve was placed before a 1/8” (3.175 mm) Nalgene rubber tube (ThermoFisher Scientific, Waltham, MA, USA), through which the air flow was adjusted to ∼ 0.8 L min-1 through a series of clamps ...
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bioRxiv - Microbiology 2023Quote: ... and counterstained with 4′,6-diamidin-2-phenylindole (DAPI, 4 μg mL-1, Thermo Fisher Scientific, MA, USA). From the 18 biofilms from each year ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... After neutralization with 1/10 volume of 1 M sodium phosphate buffer (pH 8) the antibody was coupled to M270 Epoxy Dynabeads (Thermo Scientific) in ammonium sulfate buffer (0.1 M sodium phosphate pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained with primary antibodies: cytokeratin-8 (1:100; TROMA-I, DSHB) and cytokeratin- 14 (1:250; Invitrogen, PA5-16722) and with species-specific fluorochrome-conjugated secondary antibodies (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... Plates were blocked on the following day for 1hr with TBS-TMS (20mM Tris pH 8, 150mM NaCl, 0.05% Tween 20, 1% non-fat dry milk, 1% goat serum (Gibco 16210-072)) ...
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bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
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bioRxiv - Microbiology 2021Quote: ... mixed at an 1:1 ratio in a test tube and added to wells of an 8-well slide (Thermo Fisher) coated with poly-lysine (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... then lysed in Triton lysis buffer (1% Triton, 150 mM NaCl, 50 mM Tris pH 8, 1× Halt protease-inhibitor cocktail (Thermo Scientific)) ...
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bioRxiv - Biophysics 2023Quote: ... and U-251 cells were seeded onto PLL-PEG-RGD-coated 8-well chambers in the respective medium with penicillin (1 unit/mL) and streptomycin (1 µg/mL; Gibco, Life Technologies) at densities between 0.5 x 104 to 2.5 x 104 cells/well ...
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bioRxiv - Cell Biology 2020Quote: ... 4.5 μg psPAX2 and 3 μg pM2G in Opti-MEM reduced serum media (Thermo Fisher, Waltham, MA) was added to 250 μl PEI mix (44.4 μM PEI ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Pathology 2019Quote: DBA/2-PMLECs were plated in a Lab-Tek chamber slide system made of Permanox containing 8 wells (3 ×104 cells/well) (Thermo Fisher Scientific - 177 455). Afterwards ...
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bioRxiv - Microbiology 2019Quote: ... Samples were mixed with an equal volume of NuPAGE® Tris-glycine native sample buffer (Novex®) and loaded on to NuPAGE™ 3-8% Tris-acetate gel (Thermofisher, USA). Gel blots were run at 150 V for 3 hours ...
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bioRxiv - Neuroscience 2019Quote: 3 × 104 A4 and untransfected SH-SY5Y cells were seeded into 8-well Lab-Tek®II Chamber Slides (Nalge Nunc, Naperville, IL, U.S.A) and left for 48 h to strongly adhere ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 µg of protein was mixed with NuPAGE sample reducing agent and NuPAGE LDS sample buffer and run on NuPAGE 3 to 8% Tris-Acetate gel (all Thermo Fisher Scientific, Abingdon, UK). Protein was electrotransferred onto a 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: MEF R1441C cells were plated on 8-well dishes at 3×104 cells per each well (Nunc™ Lab-Tek™ II Chambered Coverglass from Thermo Fisher Scientific). Cells were cultured overnight with complete media including 10% FBS ...