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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Quantitative RT-PCR reactions were prepared with SYBR select master mix (Applied Biosystems, 4472919). The sequences of the different primers used are listed in Supplementary Table S4 ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 ug total RNA was resuspended in 87 µl RT-PCR grade water (ThermoFisher), and 3 µl exonuclease T with 10 µl buffer 4 (NEB ...
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bioRxiv - Neuroscience 2022Quote: Quantitative RT-PCR was performed with SYBR™ Green (applied biosystems, Thermo Fisher Scientific) and custom synthesized oligonucleotide primers from Gene Link ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were then run on an Applied Biosystems QuantStudio 3 RT-PCR Machine (ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed using SYBR Green or TaqMan Universal PCR MasterMixes (Applied Biosystems) on QuantStudio 7 Flex Real time PCR system (Applied Biosystems) ...
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bioRxiv - Pathology 2022Quote: ... and subsequent RT-PCR was conducted using the Superscript II Reverse Transcriptase kit (Invitrogen). Samples were incubated at 42°C for 50 min followed by incubation at 70°C for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR analysis were performed with 2X SYBR Green PCR Master mix (Applied Biosystems) on a 7500 Fast Real time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... and SuperScript III One-Step RT-PCR System with Platinum Taq DNA polymerase (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The RT-PCR product was cloned in the Zero blunt Topo vector from Invitrogen. After the sequence was verified ...
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bioRxiv - Physiology 2022Quote: ... Quantitative RT-qPCR -was carried out using TaqMan real-time PCR (7900HT, Applied Biosystems). All gene probes and master mix were purchased from Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... qPCR reactions were performed using StepOne Plus RT-PCR equipment (ThermoFisher, Carlsbad, CA, USA) according to the manufacturer’s instructions for 40 cycles ...
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bioRxiv - Pathology 2023Quote: ... RT-PCR was performed using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems) and analyzed using a Step-One-Plus Real-Time PCR system (Applied Biosystems).
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed on a 7500 Real Time PCR System from Applied Biosystems using universal cycling conditions (10 min at 95 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the RT-PCR products were recombined into the Gateway donor vector pDONR207 (Invitrogen). DNA fragments were introduced into the destination Gateway vectors pGBKT7-GW ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed using the QuantStudio 6 Flex detection system (Applied Biosystems) and SYBR Green (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR was performed using SYBR Green master mix (Applied Biosystems, USA, Cat# 4367659) using specific primers for IL-6 ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using Power SYBR Green PCR Master Mix (4368708, ThermoFisher Scientific) on a Step One Plus Real-Time PCR machine (Applied Biosystems) ...
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bioRxiv - Genetics 2023Quote: ... RT-qPCR analysis was performed in a 7500 Real Time PCR System (Applied Biosystems) using the SYBR® Green Reagent protocol (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed on synthesized cDNA using a QuantStudio6 RT-PCR system (Applied Biosystems). Relative expression levels were quantified by the ΔΔCt method using the qPCR primers described above ...
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bioRxiv - Biochemistry 2023Quote: ... RT-PCR was performed using SYBR green dye (Thermo Fisher Scientific cat. no. 4367659) and a Bio-Rad C1000TM Thermal Cycler ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed with a StepOnePlus™ Real-Time PCR System (Applied Biosystems). Standard cycling conditions were 10 min at 95 °C followed by 40 cycles of 15 s at 95 °C and 1 min at 60 °C ...
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bioRxiv - Physiology 2023Quote: ... RT-PCR reactions were run on the SimpliAmp thermocycler (Applied Biosystems, Foster City, CA) using an equivalent to 100 ng of RNA and specific primers for GAPDH2 ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR amplifications were performed using SYBR® Green PCR Master Mix (Applied Biosystems) on a StepOnePlus™ thermocycler (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: We used SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen 18080-051) for cDNA synthesis ...
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bioRxiv - Genetics 2023Quote: ... RT-PCR was performed using the DreamTaq Green DNA Polymerase (Thermo Scientific™, USA) and the PCR products were running in the 1.2% agarose gel ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCR was performed in the QuantStudio 3 Real-Time PCR (Thermo Fisher) and used SyberGreen to monitor double-stranded DNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) in a LightCycler480 (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... and reverse-transcribed using the ThermoScript RT-PCR kit (Thermo Fisher Scientific, CA, USA). The qRT-PCR reactions were set up with FastStart DNA Master SYBR Green I master mix (Takara ...
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bioRxiv - Microbiology 2023Quote: ... we performed RT-qPCR using the SYBR Green PCR Master Mix (Applied Biosystems #A25742) following the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was carried out using SYBR Green real-time PCR master mixes (Invitrogen) and the StepOnePlus Real system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time RT-PCR analysis was performed using a StepOnePlus System (Thermo Fisher Scientific) and THUNDERBIRD Next SYBR qPCR Mix reagents (TOYOBO ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by cDNA synthesis using SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR was performed in 20 μl reactions in 96-well plates (ThermoFisher:AB0800W) using BRYT Green Dye contained in the GoTaq 1-Step RT-qPCR kit (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was carried out on a StepOne Real-Time PCR System (Applied Biosystems) according to the following programme ...
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bioRxiv - Cell Biology 2020Quote: ... mimics for murine miR-147-3p or miR-298-5p and Lipofectamine® RNAiMAX Reagent were resuspended with Opti-MEM® Medium (Gibco, Waltham, MA, USA) and incubated for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from 5 ng of total RNA using TaqMan® Small RNA Assays RT-primers (Thermo Fisher Scientific) with the High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Physiology 2020Quote: ... and 0.5 μg of RNA was reverse transcribed with random hexamer primers using a High-Capacity cDNA RT kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Approximately 200 ng of extracted vRNA was reverse transcribed using a universal 3′ primer (5′-AGGGCTCTTCGGCCAGCRAAAGCAGG) and Superscript III reverse transcriptase (RT) (Invitrogen). The RT product was diluted approximately 10,000-fold and used as a template for quantitative PCR (qPCR) ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed with 5x RT-buffer mix with M-MuLV- RH reverse transcriptase (Biolabmix) and random hexamer primer (Invitrogen) and diluted 1:10 in MilliQ H2O ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized using DNA-free RNA with oligo-dT (20) primers and SuperScript III First-Strand Synthesis System for RT-qPCR (Invitrogen). After incubation at 50°C for 1 h and 70°C for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using oligo-dT primer and Superscript III reverse transcriptase (RT) in a 20 μl reaction (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... then reverse transcribed into cDNA using a TSO primer (59-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-39) and RT maxima reverse transcriptase (Thermo Fisher Scientific). Amplification of cDNA was performed using an ISPCR primer (59-AAGCAGTGGTATCAACGCAGAGT39 ...
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bioRxiv - Neuroscience 2021Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using oligo(dT) primer and Superscript IV reverse transcriptase (RT) according to the manufacturer’s instructions (Life Technologies). First-strand cDNA samples were diluted 1:3 and 5 µL was used as template ...
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bioRxiv - Neuroscience 2019Quote: ... First-strand cDNA was synthesized from 2 micrograms of RNA using oligo(dT) primer and Superscript IV reverse transcriptase (RT) according to the manufacturer’s instructions (Life Technologies). First-strand cDNA samples were diluted 1:5 and 5 μl was used as template ...
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bioRxiv - Immunology 2020Quote: ... 100 ng to 1 µg total RNA was subjected to reverse transcription using random primers and M-MLV RT (Invitrogen). Specific primer pairs for each gene were designed ...
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bioRxiv - Genetics 2020Quote: ... First-strand cDNA was synthesized from 2 micrograms of RNA using oligo(dT) primer and Superscript IV reverse transcriptase (RT) according to the manufacturer’s instructions (Life Technologies). First-strand cDNA samples were diluted 1:10 and 5 μl was used as template ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using 1 ug of RNA reverse transcribed for 50 min at 42°C using oligo(dT) primer (IDT) and SuperScript II RT (Invitrogen). For qPCR analysis ...
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bioRxiv - Microbiology 2022Quote: ... Extracted vRNA was reverse transcribed using a 1:1 ratio of universal influenza primers (53)(S2 Table) and Maxima RT (Thermofisher) per protocol instructions ...
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bioRxiv - Genomics 2023Quote: ... 23 μL were mixed with reverse transcription master mix (1.25 μL of 10 μM scc primers, 10 μL Maxima RT buffer (ThermoFisher #EP0753), 5 μL dNTPs (New England Biolabs #N0447L) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was converted to cDNA using multiplexed RT primers and High Capacity cDNA Reverse Transcription Kit (ThermoFisher; Carlsbad, CA). miRNAs were then quantified using individual TaqMan assays on an ABI QuantStudio 7 real time PCR machine (Applied Biosystems) ...