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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... RT-PCR reactions were performed in a 20 μL mixture containing 1× Power SYBR™ Green PCR Master Mix (Thermofisher Scientific), 0.5 μmol/L of each primer ...
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bioRxiv - Immunology 2023Quote: ... Quantitative polymerase chain reaction(PCR) was conducted with PerfeCTa SYBR-Green FastMix-Rox(Quanta-Biosciences) and the StepOnePlus rt-PCR system(Applied Biosystems). We used the 2-ΔCT method for statistical analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription PCR (RT-PCR) was carried out after cDNA synthesis using the High Capacity cDNA reverse transcription kit (Applied Biosystems). Real-time quantitative reverse transcription PCR (qRT- PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... was synthesized from 1000 ng of RNA using the Maxima First Strand cDNA Synthesis Kit for PCR with reverse transcription (RT–PCR) (Thermo Fisher). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-quantitative Reverse Transcription PCR (RT-qPCR) was carried out using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, USA) and primers for germ layer differentiation and pluripotency marker genes (Suppl ...
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bioRxiv - Genomics 2023Quote: The real-time quantitative PCR (RT-qPCR) assays were performed using the QuantStudieo 5™ Real-Time PCR System (Applied Biosystems). Amplifications were carried out in 25 μL reaction solutions containing 12.5 μL 2x PerfeCTa SYBR green SuperMix (2X ...
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bioRxiv - Bioengineering 2023Quote: ... Total RNA was then reverse-transcribed to cDNA and real-time polymerase chain reaction (PCR) was performed using the Applied Biosystems StepOne Plus RT-PCR with SYBR Green Master Mix (Applied Biosystems). Murine gene names and primer sequences are listed in the Supporting Information (Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression levels were determined by quantitative real-time polymerase chain reaction (RT-PCR) (StepOnePlusTM Real-Time PCR System; Applied Biosystems) with the 2-ΔΔCT method [35] ...
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bioRxiv - Developmental Biology 2021Quote: ... Excess of primers and dNTPs were then removed using ExoSAP-IT PCR Cleanup Reagents (ThermoFisher). Samples (3 ng ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and PCR primers were used for bidirectional sequencing on a 3130xl Genetic Analyzer (Applied Biosystems). Sequences were edited using CodonCode Aligner (CodonCode Corporation ...
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bioRxiv - Molecular Biology 2021Quote: ... with gene-specific primers using PowerUp SYBR Green PCR Master Mix (Thermo Fisher Scientific, USA). The details of the primers (sequences and annealing temperatures ...
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bioRxiv - Immunology 2021Quote: ... with specific primers (Metabion) on an Applied Biosystems StepOne Real-Time PCR system (Applied Biosystems). Relative gene expression was calculated after normalization to β-actin transcriptional levels.
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bioRxiv - Immunology 2021Quote: ... Gene expression was quantified with TaqMan real-time PCR primers (Thermo Fisher Scientific, Waltham, MA). The results were normalized against β-actin.
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bioRxiv - Plant Biology 2020Quote: ... re-amplified by PCR with primers with attB tails and BP-cloned into pDONR207 (Invitrogen). Promoter:GUS binary vectors were generated by LR recombination using Gateway LR clonase II (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers (STable 2) and SYBR Green PCR Master Mix (Applied Biosystems by Life Technologies, TX) were used for q-PCR analysis using the StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers (STable 2) and SYBR Green PCR Master Mix (Applied Biosystems by Life Technologies, TX) were used for q-PCR analysis using the StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... carried out on a BioRad CFX96 Real-Time System using TaqMan PCR primers (ThermoFisher Scientific) according to manufacturer instructions using 50ug of cDNA per reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The qRT-PCR for mRNAs of interest was performed using gene-specific primers (Applied Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Table S1) and all PCR primers were purchased from Thermo Fisher (Waltham, MA; Table S2). All protocols involving animals or using animal tissue have been approved by Brigham and Women’s Hospital Institution of Animal Care and Use Committee (IUCAC) ...
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bioRxiv - Microbiology 2019Quote: ... Primer 2: 5’-GGATGTTCGTCCAGTGAGATTAG-3’) using a 7500 Fast Real-Time PCR System (Applied Biosystems) and accompanying software to analyze qPCR data.
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bioRxiv - Microbiology 2021Quote: ... amplified with indicated primers using the Power SYBR Green PCR Master Mix (Thermo Fisher, # 4367659), and analyzed by QuantStudio 5 Real-Time PCR (Thermo Fisher) ...
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bioRxiv - Pathology 2021Quote: ... housekeeping control Bm-tub primers were used with SYBR Green real-time PCR (Applied Biosystems) per manufacturer specifications (14).
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bioRxiv - Cancer Biology 2020Quote: ... 150nM of each primer and 5 μl 2X-Jump Start SYBR Green PCR Mix (Invitrogen) in10 μl total volume ...
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bioRxiv - Cancer Biology 2019Quote: ... and 0.4 μM of primers using a QuantStudio 6 real-time PCR system (Applied Biosystems). All qPCR primers are listed in the dataset provided in Synapse database ...
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bioRxiv - Genomics 2021Quote: ... amplified with indicated primers using the Power SYBR Green PCR Master Mix (Thermo Fisher, # 4367659), and analyzed by QuantStudio 5 Real-Time PCR (Thermo Fisher).
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bioRxiv - Physiology 2022Quote: ... each specific primer at 200 nM and SYBR Green PCR Master Mix (Thermo Fisher Scientific), following the manufacturer’s conditions ...
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bioRxiv - Cell Biology 2023Quote: To remove leftover primers 6 μL ExoSAP-IT For PCR Product Clean-Up (Affymetrix – 78200) was added and the sample was incubated at 37°C for 15 min and then chilled and kept at 4°C.
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bioRxiv - Cell Biology 2023Quote: ... Nextera primers matching those used in the first PCR step and SYBR Green I (Invitrogen), the following qPCR program was performed ...
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bioRxiv - Cancer Biology 2024Quote: ... and following probes and primers on a 7300 Real-Time PCR System (Applied Biosystems, CA). Primer/probes for mouse TNF-a gene were purchased from Applied Bioscience (Mm00443258_m1) ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time qRT-PCR reactions were set up using FastStart Universal SYBR Green Master Mix (Thermo Fisher Scientific) and thermal cycling was performed using a StepOnePlus (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... PCR products (0.4 μL of each set) were added to 10 μL of HiDi™-Formamide (Applied Biosystems), containing 0.3 μL of the GeneScan™HD 500 ROX™ Size Standard (Applied Biosystems) ...
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bioRxiv - Physiology 2022Quote: ... and qPCR reactions were set up and performed on the StepOne-Plus Real-Time PCR System (Applied Biosystems) as previously described (Wilson ...
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bioRxiv - Microbiology 2019Quote: ... / Bac1115Probe (5’-CAACGAGCGCAACCC-3’) primer and probe set targeting a conserved region of bacterial 16S rRNA genes using a QuantStudio3 platform (Thermo Fisher Scientific, Waltham, MA) (ref ...
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bioRxiv - Neuroscience 2020Quote: ... Synthetic oligos were cloned into the GW/EmGFP-miR vector (Invitrogen), which contains sequences derived from miR-155 (see Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... We also measured miR-375-3p (Thermo Fisher Assay ID #00564), which was not included on the array ...
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bioRxiv - Cancer Biology 2019Quote: ... Anti-miR-146a (AM-34a) (ID: AM11030) were obtained from Ambion, CD24 siRNA (a pool of 3 target specific siRNAs ...
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bioRxiv - Neuroscience 2021Quote: ... The probes hsa-miR-499a-5p (Alexa Fluor 546; Thermo Fisher) and CamK2 (Alexa Fluor 488 ...
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Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancerbioRxiv - Cell Biology 2022Quote: ... Cells were transfected using either a miR-206 miRNA mimetic (Thermofisher) or negative control mimetic #1 (Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... 10-nM mirVana hsa-miR-21 specific inhibitor (Thermo Fisher Scientific), or mirVana miRNA inhibitor Negative Control (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and miR inhibitor negative control (CAT#4464076) were purchased from Ambion/Life Technologies.
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected using either a miR-206 miRNA mimetic (Thermofisher) or negative control mimetic #1 (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... synthetic cel-miR-39-3p (Thermo Fisher Scientific, Waltham, MA, USA) was added to samples during the cell/MP lysis step as a spike-in control ...
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bioRxiv - Cancer Biology 2023Quote: For the functionality validation of miR-34a mimic (MC11030, Thermo Fisher), PC3 cells were seeded onto individual wells of a 96-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... and miR-140-5p mimics and corresponding scrambled control (SC) (Ambion) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... The coding sequence of OsSWEET11b was PCR amplified without STOP codon (primers: Table S1) using Phusion High-Fidelity PCR polymerase (Thermo Fisher Scientific) and cloned into the Gateway donor vector pDONR221 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Diluted cDNA was used for qRT-PCR using gene specific primers (Table S2) and Power SYBR Green PCR Master Mix (Applied Biosystems, A25742). Gapdh was used as an endogenous control.
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bioRxiv - Cancer Biology 2022Quote: ... This single-stranded cDNA was then used to perform quantitative real-time PCR (qRT-PCR) with specific primers using PowerUP SYBR Green 2x Master Mix (Thermo Fisher Scientific) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR amplification was performed with barcoded reverse PCR primers in 25 cycles in Taq DNA Pol reaction buffer 1 × (Thermo Fisher Scientific), with 1 μM of each reverse barcoded primer and forward primer (Table SI1) ...
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bioRxiv - Cell Biology 2023Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...