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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... mRNA gene expression was determined using SYBR Green RT-PCR on the cDNA template and PCR was performed using StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, Massachusetts, USA). The mRNA expression of each gene was evaluated using the comparative Ct (ΔΔCT ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative reverse-transcription PCR (q-RT-PCR) was performed on a Step One Plus Real Time PCR System (Applied Biosystems, Foster City, CA, USA) using TaqMan gene expression assays (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Organoids were then carefully transferred using FBS pre-coated wide orifice p200 pipette tips (Fisher Scientific, 02-707-134) into an ultra-low attachment 24-well plate (Corning ...
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bioRxiv - Immunology 2021Quote: ... and Cg specific primers in a 20 μl PCR reaction using High Fidelity Platinum PCR Supermix (Life Technologies, Carlsbad, CA) and amplified by 40 cycles ...
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bioRxiv - Molecular Biology 2020Quote: The PCR products were purified to remove unincorporated primers and dNTP’s using the PCR purification kit (Purelink PCR purification kit, Invitrogen, USA). The purified products were processed for sequencing on a next generation sequencer platform @ 40X coverage (MiSeq ...
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bioRxiv - Genomics 2020Quote: ... PCR were performed with primers ordered from IDT using either Phusion U DNA Polymerase Green MultiPlex PCR Master Mix (ThermoFisher) or Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... real-time quantitative PCR analysis with a gene specific primer pair using SYBR green PCR mix (Applied Biosystems, cat: 4385612) was performed and data was analysed on the Applied Biosystems ViiATM 7 Real-Time PCR System ...
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bioRxiv - Microbiology 2022Quote: ... These 2 reaction products were then combined in a second PCR reaction with primer pairs 53 + 56 and the resultant DNA cloned into pCR-Blunt (Invitrogen) before being excised using XbaI + EcoRI and cloned into XbaI + EcoRI cut pSGRJFH1luc ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Primer to be used for quantitative reverse transcription-PCR (qRT-PCR) were designed using the Primer Express 3.0 software (Applied Biosystems) and efficiencies were validated prior to use ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinesin-1 motor domain was PCR amplified from cDNA using gene-specific primers and cloned into the pCR-XL-2-TOPO vector (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using specific primers (0.2µM concentration and SYBR Green PCR Master Mix (Applied Biosystems, #4309155) on the Mastercycler RealPlex2 (Eppendorf) ...
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bioRxiv - Bioengineering 2023Quote: Total miR levels in extracted RNA samples were quantified using a Qubit miR Assay Kit and Qubit 3.0 Fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA); the manufacturer’s protocols were employed.
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bioRxiv - Systems Biology 2020Quote: ... The Taqman method was used for real-time PCR with primers from Life technologies. The expression of mRNA was normalized to the geometrical mean of 3 house-keeping genes ...
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bioRxiv - Cell Biology 2020Quote: ... primers (Table 1) and Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific) were used in standard conditions and relative RNA quantification was normalized to the housekeeping gene GAPDH.
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bioRxiv - Developmental Biology 2019Quote: ... We performed qRT-PCR with bcd-specific primer pair using SYBR Green (Thermo Fisher) protocol and the housekeeping gene rpl32 was used as internal reference ...
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bioRxiv - Genomics 2019Quote: ... using TVN PCR reverse primer (5’-CAAGCAGAAGACGGCATACGATTTTTTTTTTTTTTTTTTVN-3’) and SuperScript III Reverse Transcriptase (Invitrogen), and then the reaction was stopped by heating at 70°C for 15min ...
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bioRxiv - Cancer Biology 2022Quote: ... real-time PCR for genes was performed using individual gene Taqman primers (Applied Biosystems) with an ABI 7500 real-time PCR system ...
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bioRxiv - Cancer Biology 2022Quote: ... along with gene specific primers on a 7900HT real-time PCR machine (Applied Biosystems). Statistical significance was determined by Students t test based on triplicated experiments.
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bioRxiv - Microbiology 2020Quote: ... The backbone of pENTR plasmid was amplified by PCR using primers pENTR_gibson_backbone_F and pENTR_gibson_backbone_R from the pENTR-D-TOPO cloning kit (Invitrogen). The resulting PCR product was subsequently treated with DpnI to remove methylated template DNA ...
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bioRxiv - Immunology 2022Quote: ... and target specific TaqMan real-time PCR assay primer probes (ThermoFisher Scientific, Waltham, MA). Viral burden was determined by quantitative real-time RT-PCR on lung RNA for viral matrix protein (M1 ...
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bioRxiv - Genetics 2019Quote: ... PCR-based primer panels were designed with Ion AmpliSeq™ Designer (Thermo Fisher Scientific) to amplify all exons and flanking regions (10 bp ...
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bioRxiv - Physiology 2020Quote: ... qRT-PCR experiment was performed by Taqman Gene Expression Assay primers (Thermo Fisher Scientific) in triplicates using LightCycler 480 Real-Time PCR System (Roche Diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... using TaqMan® Universal PCR Master Mix and predesigned primer pairs (Thermo Fisher Scientific) for rat Gapdh (Rn01775763_g1) ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR was performed using Taqman primers for Csf3 (Thermo Fisher Scientific Mm00438334_m1), Cxcl1 (Mm04207460_m1) ...
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bioRxiv - Molecular Biology 2022Quote: ... using TaqMan® Universal PCR Master Mix and predesigned primer pairs (Thermo Fisher Scientific) for rat Gapdh (Rn01775763_g1) ...
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bioRxiv - Plant Biology 2022Quote: ... Gene-specific PCR primers (Table S3) were used in a reaction with Dreamtaq (Thermofisher). Colonies were picked into 20 μl sterile water with a micropipette tip and resuspended ...
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bioRxiv - Developmental Biology 2023Quote: ... gene-specific primers and Power SYBR Green PCR Master Mix (Applied Biosystems, UK, #4309155) with a Bio-Rad C1000Touch™ Thermal Cycler ...
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bioRxiv - Cell Biology 2024Quote: ... 200 nM of primers and Power SYBR™ Green PCR Master Mix (Applied Biosystems) in a BioRad thermocycler ...
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bioRxiv - Immunology 2021Quote: ... followed by specific TaqMan® miR-34a Assay (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Ambion pre-miR miRNA precursor molecules (Thermo Fisher Scientific, UK) were used for transient miR-187 overexpression ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with hsa-miR-3p mimic (Invitrogen) or a control mimic at 5 nM final concentration using Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... or hsa-miR-106b-3p mirVana™ miRNA Mimic (Ambion) at final concentration of 5 nM ...
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bioRxiv - Neuroscience 2021Quote: ... miRNA mimics (Ambion™ Pre-miR miRNA Precursor, Thermo Fisher), and pLNAs (miRCURY LNA miRNA Power Inhibitors ...
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bioRxiv - Neuroscience 2022Quote: ... and hsa-miR-132 (Applied Biosystems miRNA assay ID 000457).
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bioRxiv - Neuroscience 2023Quote: ... or respective quantities of pre-miR negative control duplex (Ambion) at DIV9 ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR reaction was set by using TaqPath 1-Step Master Mix (Cat. No. A28527; Thermo Fisher) with a primers and a FAM-labeled probe targeting the N1 amplicon of N gene (2019-nCoV RUO Kit ...
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bioRxiv - Microbiology 2019Quote: ... The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies, Carlsbad, CA) in a total volume of 50 µl ...
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bioRxiv - Microbiology 2022Quote: ... The cycling conditions were set in the PikoReal™ Real-Time PCR System (Thermofisher Scientific, Waltham, MA) as follows ...
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bioRxiv - Neuroscience 2023Quote: ... Amplification reactions (triplicates) were set up using Power up SYBR Green PCR Master Mix (Thermo Fisher Scientific) and qRT-PCR was validated with the QuantStudio 1 Real Time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Amplification reactions (triplicates) were set up using the PowerSYBR Green PCR Master Mix (Thermo Fisher Scientific, 4367659) and qRT-PCR was performed using the QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and the amplification of RT-PCR was performed with Superscript III Platinum One-Step reagents RT-qPCR System (Cat. 11732-088, Invitrogen, Carlsbad, CA, USA). The Applied biosystems 7500 fast platform was used in all cases ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was used to detect and determine virus titers using the AgPath ID one-step RT-PCR kit (ThermoFisher Scientific, Waltham, MA) from the cell culture supernatants as previously described [28] ...
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bioRxiv - Plant Biology 2020Quote: ... the ViN vectors and housekeeping genes was quantified using qRT-PCR performed with the one-step SuperScript RT-PCR kit (Invitrogen) on a BioRAD thermocycler ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using the 7500 Fast Real-time PCR System (Applied Biosystems by Life technologies, Paisley, UK) with the SYBR Select Master Mix for CFX (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using the 7500 Fast Real-time PCR System (Applied Biosystems by Life technologies, Paisley, UK) with the SYBR Select Master Mix for CFX (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... and ICV using real-time RT-PCR on a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) using previously reported primer and probe sets (Supplemental Table A ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was reverse transcribed for quantitative RT-PCR (qRT-PCR) experiments by adding 10µg of total RNA to a mixture containing random hexamers (Life Technologies), 0.01M dithiothreitol ...
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bioRxiv - Immunology 2022Quote: ... was used and results were analyzed by quantitative RT-PCR with a Vii 7 Real-time PCR system (Applied Biosystems). The comparative threshold cycle method and internal control (Gapdh ...
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bioRxiv - Molecular Biology 2020Quote: ... all samples were analyzed using the Applied Biosystem 7300 Fast Real-Time PCR (RT-PCR) System (Life Technologies, California, USA). RT-PCR analysis was performed in a 96-well plate ...