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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with Fast SYBR Green Master mix (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT-qPCR was performed in an ABI OneStep Plus Real-time PCR System (Applied Biosystems) from 50 ng RNA using the qPCRBIO SyGreen 1-step Go Hi-ROX System from (PCRBiosystems) ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was carried out using the ABI StepOnePlus thermocycler (Life Technologies, Grand Island, NY). The S-N501Y ...
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bioRxiv - Microbiology 2024Quote: ... RT-PCR products were quantified on a Qubit 4 Fluorometer using HS DNA reagents (Invitrogen). A volume of 3.5 µL of the cDNA product was used in a 50 µL PCR reaction with Phusion™ High-Fidelity DNA Polymerase (2 U/µL ...
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR was performed using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems) in duplicate using the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR reactions were performed in triplicate using PowerUp SYBR Green Master Mix (Thermo Fisher). cDNA amplification was performed for 40 cycles on a QuantStudio 3 (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were performed on QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) and analyzed using QuantStudio 12K Flex Applied Biosystems software v1.2.3 ...
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bioRxiv - Microbiology 2023Quote: ... Two-step RT-qPCR was performed on a StepOnePlus real-time PCR system (ThermoFisher Scientific) using the high-capacity RNA-to-cDNA kit (4387406 ...
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bioRxiv - Developmental Biology 2024Quote: ... The RT-PCR product was cloned into the TOPO® TA cloning pcr4 vector (Invitrogen) and sequenced ...
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bioRxiv - Synthetic Biology 2023Quote: ... using 126 ng DNA and 420 ng PEI in 16.8 μL OptiMEM medium (Life Technologies, Carlsbad, CA, cat. no. 22600-134) per well of a 96-well plate ...
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bioRxiv - Genomics 2019Quote: ... RT-PCR was performed with 1µl of template cDNA and Maxima Hot Start Green PCR Master Mix (ThermoFisher Scientific, Cat No-K1061). The cycling conditions were ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting cDNAs were subsequently used for two-step quantitative RT-PCR with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) on a 7500HT Fast Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... and qRT-PCR was performed using SYBR FAST Universal 2X qPCR Master Mix (Kapa) on a QuantStudio 3 RT-PCR System (Thermo Fisher Scientific). All samples were run in triplicate and the CT value was normalized to calculate relative expression of each gene ...
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bioRxiv - Immunology 2021Quote: ... 500 ng of RNA was used for the RT-PCR and the qPCR was performed using QuantStudio 6 PCR System (Thermo Fisher Scientific). The primer sequences were as follows ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (RT-qPCR) was performed using SYBR Green dye-based assay in a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) with the following reaction conditions ...
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bioRxiv - Microbiology 2021Quote: ... The presence of SARS-CoV-2 RNA was determined by conventional reverse transcription PCR using the SuperScript®III One-Step RT-PCR System with Platinum® Taq DNA Polymerase kit (Invitrogen) in a 25μl reaction volume containing 12.5μl of 2X buffer ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 subgenomic RNA of the E gene was reverse transcribed and copy numbers quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and viral genome copies quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: One-step RT-PCR was performed on 2ul total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232-1583 of the PPRV N gene using published primers NP3 and NP4 (Couacy-Hymann et al 2002 ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR was used for mRNA quantification (7500 Fast Real-Time PCR system, Applied Biosystems Inc., Foster City, California, USA). All primers were designed to cover exon-exon boundaries to avoid amplification of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... The amount of the intracellular RNA was estimated by real-time quantitative PCR performed with the RNA UltraSense™ One-Step Quantitative RT-PCR System (Applied Biosystems) by using the following primer sets ...
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bioRxiv - Immunology 2019Quote: ... Real-time RT-PCR was used for mRNA quantification (7500 Fast Real-Time PCR system, Applied Biosystems Inc., Foster City, California, USA). All primers were designed to cover exon-exon boundaries to avoid amplification of genomic DNA ...
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bioRxiv - Biochemistry 2021Quote: ... The viral titers were determined by the qRT-PCR method using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific) and a sequence of primers and probe for the E gene53 ...
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bioRxiv - Neuroscience 2021Quote: We performed gene expression analysis by quantitative real-time RT-PCR (qPCR) using SYBR Green PCR Master Mix (Thermo Fisher Scientifics, #4309155) and the ViiA™ 7 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNA was subjected to reverse transcription PCR for cDNA synthesis according to the RT-PCR kit (Applied Biosystems, Cat. No. 4368814). The relative gene expression was measured according to 2-ΔΔCT methods ...
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bioRxiv - Genetics 2022Quote: ... The SARS-CoV-2 infection was screened and confirmed by at least one quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) assay with the Applied Biosystems 7500 Fast RT-PCR System (Thermo Fisher Scientific), using a protocol previously described for the detection of 2019 novel Coronavirus (2019-nCoV ...
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bioRxiv - Synthetic Biology 2022Quote: ... situated between nsP4 and the viral 3’ untranslated region (UTR) were reverse-transcribed and PCR-amplified using the SuperScript™ IV One-Step RT-PCR System (ThermoFisher, #12594025) and the 26S-F primer and pooled SinRev primer ...
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bioRxiv - Microbiology 2022Quote: ... The viral cDNA was amplified from extracted RNA through one-step reverse transcription-PCR amplification by using SuperScript III One-Step RT-PCR system with High Fidelity Platinum Taq DNA Polymerase (Invitrogen, Life Technologies, USA) with degenerate primers (10uM MBTuni-12M and MBTuni-13 ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR was performed using 1 µl of eluted RNA and a SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) as described by the manufacturer.
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription quantitative PCR (RT-qPCR) assays were performed using the Quantstudio 5 Real-time PCR system (Applied Biosystems, Waltham, MA, USA). Premixes were prepared for each amplification reaction using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted with NucliSens easyMAG (BioMérieux) and quantified by quantitative real time PCR (RT-qPCR) using SuperScript™ III Platinum™ One-Step qRT-PCR Kit (Invitrogen) in a CFX96 Thermal Cycler (BIORAD) ...
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bioRxiv - Microbiology 2023Quote: ... real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using Taqman probe (Mm00458221 ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-Time quantitative PCR (RT-qPCR) was performed on the QuantStudio™ 5 Real-Time PCR System 384-well (Applied Biosystems, #A28140) using SenSMix™ SYBR® No-ROX buffer (Meridian Bioscience) ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with duplicates on a MicroAmp™ Fast Optical 96-Well Reaction Plate (Themo Fisher Scientific) using the QuantStudioTM 5 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
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bioRxiv - Physiology 2023Quote: ... Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science) with the StepOnePlus Real-time PCR System (Thermo Fisher Scientific), as in our previous studies (26 ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was reverse transcribed using Superscript III One-Step RT PCR Kit with Platinum® Taq High Fidelity enzyme followed by amplification using Platinum® PCR SuperMix High Fidelity (Invitrogen). A 2200bp amplicon spanning gag and protease was generated by nested PCR as detailed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science) with the StepOnePlus Real-time PCR System (Thermo Fisher Scientific). Results were normalized using the means of Actb and Gapdh as reference genes ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription and PCR were performed on 35ng of total RNA per well using AgPath-ID One-Step RT-PCR Reagents (4387424, Thermo Fisher Scientific) with the following TaqMan primer-probe sets (all Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time reverse-transcription PCR (RT–qPCR) assays were performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). The primer sequences were listed in S1 Table ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR of the cDNA was performed using a primer set for the N gene of RSV (45) and ACTB (Applied Biosystems, TaqMan Assay ID: Hs99999903_m1) as a housekeeping gene ...
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bioRxiv - Genomics 2023Quote: ... qPCR reactions were set up in PowerUpTM SYBRTM Green PCR master mix (Thermo Scientific, PA, USA, Cat No. A25743) with gene specific primers (Table 1) ...
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bioRxiv - Plant Biology 2020Quote: ... and primers in 10 μL of reaction and an ABI7500 real-time PCR system (Life Technologies). The expression of each gene was normalized to the expression of Actin and quantified using the 2-ΔCt method ...
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bioRxiv - Bioengineering 2020Quote: ... and primer-probe assays (IDT) on a Step One Plus Real-Time PCR System (Applied Biosystems). The AR-V7 primer-probe assay included ...
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bioRxiv - Immunology 2019Quote: ... qRT-PCR was performed using commercially available Taqman primer/probes for cd5 and ubc (Thermo Fisher).
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bioRxiv - Microbiology 2019Quote: ... qPCR was performed using specific primers and the SYBR Green PCR master mix (Applied Biosystems, USA) in a 20 μl reaction system ...
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bioRxiv - Genetics 2019Quote: ... and 0.2 µM each primer in a 7500 Fast Real-Time PCR System (Applied Biosystems, USA). RT-qPCR conditions were as follows ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Primer/Probes was run on QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) with standard settings ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR was performed with target-specific primers using the TaqMan Gene Expression Assay (Applied Biosystems) or the Fast SYBR Green Assay (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... and the primers listed in table S4 on a Quantastudio Real-time PCR instrument (Applied Biosystems). ☐-actin levels were used to normalize input amounts ...