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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: For chrd WISH experiments amplified region was cloned into TOPO II dual promoter vector (Thermo scientific, USA). To generate antisense probe ...
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bioRxiv - Cell Biology 2021Quote: Tissue-specific promoters driving lbp-3 or nlp-11 vectors were generated using Multisite Gateway System (Invitrogen). Promoters were cloned to 1’ position Donor Vector pDONR 221/P1-P4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Mitfa:mCherry was Gateway recombined using the mitfa zebrafish promoter and a mCherry middle entry plasmid (ThermoFisher #12538120). Ubi:caSMAD2 was cloned by PCR amplifying human SMAD2 pDONR221 (DNASU HsCD00045549 ...
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bioRxiv - Genetics 2024Quote: ... efk-1 promoter and genomic sequence were cloned into Zero Blunt™ TOPO™ vector (Invitrogen 450245), transformed into TOPO One Shot™ MAX Efficiency DH5α-T1R E ...
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bioRxiv - Cell Biology 2023Quote: ... the promoter reporter vector and the pRL-CMV Renilla luciferase plasmid using Lipofectamine 2000 reagent (Invitrogen, USA). After transfection for 24 h ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected with 0.5 pmol of pGL4.54[luc2_TK] and 0.05 pmol of the promoter-test vectors per well with Lipofectamine 3000 (Invitrogen). Luciferase activity was assessed 48h post-transfection (72h post siRNA transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... The FLIP-promoter-luciferase plasmids were transiently transfected with lipofection 2000 (Life Technologies, catalog no. 11668-027) into cancer cells as stated in the figures for 24 hours ...
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bioRxiv - Genetics 2021Quote: ... Selected disease-associated variant findings were genotyped using standard capillary sequencing on an ABI3730xl DNA Analyzer platform (Thermo Fisher Scientific, Waltham, MA, USA) as a secondary technology to provide further validation of results that were unexpected for the breed ...
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bioRxiv - Cell Biology 2022Quote: ... of the standard curve used in cf-mtDNA/cf-nDNA assessment were measured separately using singleplex ND1 and B2M assays using a QuantStudio 3D Digital PCR System and associated reagents (Thermo Fisher, cat#A29154) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... of the standard curve used in cf-mtDNA/cf-nDNA assessment were measured separately using singleplex ND1 and B2M assays using a QuantStudio 3D Digital PCR System and associated reagents (Thermo Fisher, cat#A29154) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT PCR reaction was run on a QuantStudio(TM) 7 Flex System in a 96 well format with associated software performing analysis (Applied Biosystems Cat. 4485701).
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bioRxiv - Bioengineering 2022Quote: ... bacteria were captured using commercially available beads with their associated magnetic separator device (anti-E. coli O157 Dynabeads®, DynaMag™ -2 magnet, Life Technologies) following the manufacturer’s guidelines for use ...
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bioRxiv - Cell Biology 2022Quote: All PURE translation reactions were carried out for 2 h at 32°C and then ribosome-associated nascent chains were released by addition of 1 mM puromycin (Thermo Fisher Scientific, USA) and further incubation for 10 min at 32°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the membrane-associated proteins were transferred to a new tube followed by protein concentration determination by BCA assay (Thermo Fisher, A53225/A53226). For ubiquitin immunoprecipitation at 3.4 mg per sample ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was examined by 5-bromo-2-deoxyuridine (BrdU) cell proliferation enzyme-linked immunosorbent assay (ELISA) according to the kit manufacturer’s protocol (Life Technologies, Grand Island, NY, USA). The cells were incubated in presence of various concentrations of CLEFMA (0 – 10 µM) ...
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bioRxiv - Microbiology 2021Quote: ... Fasting glucose level (measured with a glucometer; Bayer, Leverkusen, Germany) and fasting insulin level (insulin mouse ELISA kit, Thermo Fisher Scientific, Waltham, MA, USA) were measured in fasted mice.
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bioRxiv - Molecular Biology 2022Quote: ... TGF-β1 and monocyte chemotactic protein-1 (MCP-1) was measured using the ELISA kit following the manufacturer’s protocol (eBioscience Affymetrix, Santa Clara,CA, United States). The levels of Muc5ac and Muc5b in BALF were also measured according to Lu et al ...
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bioRxiv - Immunology 2022Quote: ... Murine IFNγ levels and IL-2 levels were measured using the Mouse Interferon gamma (IFNγ) Uncoated ELISA Kit (Thermo Fisher Scientific, 88-7314-76) or IL-2 Mouse Uncoated ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: TNF-α was quantified in cell culture supernatants of infected cells by Ready-SET-Go! ELISA kits (Thermo Fisher Scientific, Cat. 88-7324-77) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The total immunoglobulin E (IgE) level in the serum samples was measured using an IgE mouse ELISA kit (Thermo Fisher Scientific, Waltham, Mass, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... ratio and pERK:ERK (pT202/pY204, pT185/pY187) ratio was done using the AKT (Total/Phospho) InstantOne ELISA Kit (Thermo Fisher Scientific, 85-86046-11) for pAKT:AKT ...
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bioRxiv - Immunology 2023Quote: ... 10 μgs of protein were loaded into manually coated IL-22 96-well High Affinity plates and IL-22 levels were determined by IL-22 ELISA kit (ThermoFisher-Scientific #88-7422-22) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and nuclear PPARα in V1 were measured using ELISA kits according to manufacturer’s instructions (total AMPK: ab181422, Abcam, UK; phosphorylated AMPK: KHO0651, ThermoFisher, UK; PPARα: ab133107, Abcam, UK). For tissue collection ...
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bioRxiv - Immunology 2024Quote: ... IL-33 and IL-25 concentration was determined via Enzyme-Linked Immunosorbant Assay (ELISA) kit according to manufacturer’s protocol (IL-33: R&D Systems, DY362605; IL-25: Invitrogen, 88-7002-22).
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bioRxiv - Neuroscience 2024Quote: ... we determined the concentrations of Aβ-42 in the hippocampus and cerebral cortex tissue lysates using an ELISA kit from Invitrogen (n=6 animals/group).
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bioRxiv - Immunology 2024Quote: ... Supernatant was collected 24 hours later for analysis of IL-12p40 and IL-6 production by ELISA (Thermo Fisher Scientific Ready-SET-Go! Kit) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: Antibodies (Human Siglec-1, PE conjugated Anti-Human CD14, FITC conjugated Anti-Human CD16) were obtained from Thermo Fisher Inc ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested and cytokine concentrations measured by ELISA (Thermo Fisher) and cells were stained and analysed using flow cytometry as described below.
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested and cytokine concentrations measured by ELISA (Thermo Fisher) and cells were stained and analysed using flow cytometry as described below.
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bioRxiv - Immunology 2022Quote: ... 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific: 34029) was added for colorimetric detection ...
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bioRxiv - Immunology 2022Quote: ELISAs were performed on 96-well half-area microplates (ThermoFisher Scientific) as described previously 15 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μL 1-Step Ultra TMB – ELISA Substrate (Thermo Scientific 34028) was added to each well and incubated at room temperature for 30 mins ...
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bioRxiv - Immunology 2021Quote: ... Incubation was performed in a 96-well ELISA plate (Nunc Maxisorp) pre-treated with PBS containing10% FCS for 1 h at 4°C to avoid direct binding of serum IgG to the plate ...
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bioRxiv - Immunology 2021Quote: High binding ELISA plates (Thermo Scientific, catalog no 44-2404-21) were coated at 0.5µg/mL with goat anti-mouse IgG2b (Southern Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... The ELISA was developed using 3,5,3′,5′-tetramethylbenzidine (TMB, Thermo Scientific) solution and the reaction was stopped after 10 min incubation using 0.16M Sulfuric acid ...
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bioRxiv - Bioengineering 2021Quote: ... ELISA was performed according to manufacturer’s protocols for Ang-1 (ThermoFisher) and Ang-2 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... ELISAs were developed by addition of TMB solution (Thermo Fisher Scientific) and quenched using 10% phosphoric acid ...
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bioRxiv - Bioengineering 2021Quote: ... 100 μL of Slow TMB-ELISA substrate solution (#34024, Thermo Fisher) was added per well ...
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bioRxiv - Immunology 2020Quote: ... ELISA assays were done with material and reagents from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Nunc MaxiSorp round-bottom 96-well ELISA microtiter plates (Thermo Scientific) were coated overnight at 4°C with 10 μg/ml HSV-1 inactivated Vero cell extract (Advanced Biotechnologies) ...
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bioRxiv - Synthetic Biology 2020Quote: ... ELISAs were performed with 96-well MaxiSorp plates (Thermo Scientific, 442404).
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bioRxiv - Immunology 2020Quote: ... Incubation was performed in a 96-well ELISA plate (Nunc maxisorp) pre-treated with PBS/10% FCS (v/v ...
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bioRxiv - Microbiology 2021Quote: ... or S15 proteins were quantified by ELISA assay (Thermo Fisher Scientific) and stored at −80□.
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bioRxiv - Bioengineering 2021Quote: 96-well ELISA plates (Nunc MaxiSorp flat-bottom plates, Thermo Fisher) were coated with 10 nM RBD ...
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bioRxiv - Microbiology 2020Quote: ... the wells of 384-well ELISA plates (NUNC Maxisort cat# 464718) were coated with 15 µl of 4 µg/ml neutravidin (ThermoFisher cat # 31000 ...
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bioRxiv - Cancer Biology 2021Quote: ... One-Step™ Turbo TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added to the wells for color development ...
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bioRxiv - Immunology 2020Quote: ... Nunc high-binding ELISA plates (Thermo Fisher Scientific; Waltham, MA, USA) were coated with 2 μg/ml of recombinant SARS-CoV-2 proteins (S1 and RBD-His proteins were produced in the lab and S1 +S2 ECD spike protein was purchased from Sino Biologicals ...
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bioRxiv - Immunology 2020Quote: ... using 1-Step™ Slow TMB-ELISA (Thermo Scientific, Rockford, IL) and 2N HCl according to manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... mouse IFNβ (PBL Biomedical Laboratories) and mouse CXCL10 ELISA (ThermoFisher; BMS6018MST) were obtained from commercial sources.
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bioRxiv - Immunology 2021Quote: ... IFN-α in the culture supernatant was measured using ELISA (Invitrogen).