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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ELISA experiments were performed in 96-well plates (Maxisorp, Nunc), which were previously coated overnight at 4°C with 50 µl/well of the purified RSP protein antigen or BSA at 3 µg/ml in PBS ...
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bioRxiv - Microbiology 2024Quote: ... Nunc MaxiSorp 386-well ELISA plate (Thermofisher, 12-565-347) was coated with neat samples ...
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bioRxiv - Microbiology 2024Quote: ... Reagents and antibodies for ELISA were purchased from Thermo Fisher. The following capture antibodies were used ...
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bioRxiv - Immunology 2024Quote: ... Soft vinyl round bottom ELISA plates (Fisher scientific, Ref# 10324165) were coated with guinea pig anti-RV hyperimmune serum (kindly donated from Prof ...
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bioRxiv - Immunology 2024Quote: ... prior to collection of supernatants for IL-5 ELISA (Invitrogen).
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μL of 3,3’,5,5’ tetramethylbenzidine (TMB) ELISA substrate (ThermoFisher) were added to each well for signal development ...
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bioRxiv - Immunology 2024Quote: ... Serum was also analyzed by ELISA for mCXCL1 (Thermo Fisher) and mSAA-3 (Millipore-Sigma).
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bioRxiv - Cell Biology 2024Quote: ... were added to 96 well ELISA plate (Thermo Fisher, 612U96) and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Transcripts of interest were analyzed using the QuantStudio 5 real-time quantitative PCR (RT-qPCR) system with the associated software (Thermo Fisher Scientific). cDNA was diluted with the Fast SYBR green master mix (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used: mouse monoclonal antibody against phosphorylated microtubule-associated protein tau (p-tau, UniProtKB P10637. Thermo Fisher Scientific, MN1020), mouse monoclonal antibody against slow myosin heavy chain I (MYH1 ...
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bioRxiv - Microbiology 2021Quote: ... Measurements and analysis were performed with the Applied Biosystems™ 7500 Fast Dx Real-Time PCR Systems and associated software (Applied Biosystems). Relative gene expression was calculated using the 2ΔΔCt method (27) ...
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bioRxiv - Microbiology 2021Quote: ... Measurements and analysis were performed with the Applied Biosystems™ 7500 Fast Dx Real-Time PCR Systems and associated software (Applied Biosystems).
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bioRxiv - Cancer Biology 2021Quote: RNA was purified from 18 HIV-associated kidney cancer tumor/adjacent normal tissue samples using TRIzol LS reagent (Invitrogen, Carlsbad, CA, US) and the RNAsimple total RNA kit (Tiangen Biotech Co ...
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bioRxiv - Microbiology 2023Quote: ... The alternative commercial MRS formulation associated with aberrant cell shape (MRS*) in Bifidobacterium breve UCC2003 (Figure 5) was Oxoid brand (Thermo Fisher, CM0359). The recovery broth (RB ...
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bioRxiv - Microbiology 2024Quote: ... 30% of the immunoprecipitated protein and 70% of the associated RNA were eluted with Laemmli 1X at 95°C for 10 min or with 1 mL TriReagent (Thermo Fisher Scientific), respectively ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Microbiology 2021Quote: ... Evaluation of NF-kBp65 and CREB activation was performed in infected or uninfected monocytes using NF-kB p65 (Total/Phospho) InstantOne™ and CREB (Total/Phospho) Multispecies InstantOne™ ELISA Kits (Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Total IgG and IgM from Snca+/+ and Snca-/- were measured by commercially available ELISA kits (Invitrogen, #88-50400-22 and #88-50470-22) according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were lysed and protein normalised following content assessment by the Bradford reagent (Walker and Kruger, 2003) for use in an InstantOne™ total/phosphor multispecies p38 ELISA kit (ThermoFisher Scientific, UK) according to the manufacturer’s instructions ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: The concentration of IL-1β in GAS muscle extracts at 2-day post-surgery was measured using Mouse IL-1 β ELISA Kit (Invitrogen #88-7013), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ELISAs for mouse studies were performed for the quantitative measurement of total mouse IgG production in plasma samples using IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, #88-50400-22) for BALC/c cohorts and IgG (Total ...
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bioRxiv - Biochemistry 2023Quote: α-synuclein was quantified in the striatum region of mouse brain utilizing the Enzyme-linked Immunosorbent Assay (ELISA) Kit from Invitrogen (Cat No. KHB0061) following the manufacturer‘s instructions.
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bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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The intersection of endocrine signaling and neuroimmune communication regulates neonatal nociceptionbioRxiv - Neuroscience 2024Quote: ... The levels of MCP1 in the muscle lysate were measured by commercial MCP-1 Mouse ELISA Kit (Catalog No. BMS6005, Invitrogen Thermo Fischer Scientific). Briefly ...
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bioRxiv - Immunology 2024Quote: ... and IL-17A were measured from memory Th cell supernatants using commercially available sandwich ELISA kits according to manufacturer protocols (ThermoFisher Scientific, Burlington, Canada). Modifications included 75µL of coating and capture antibody per well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2024Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901) and Bio-Plex Pro Human MMP-2 Set (Bio-rad ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: Human genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, USA) or QIAamp DNA Blood Mini Kit (QIAGEN ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated using a commercial kit (MagniSort Human CD4+ T Cell Enrichment; Affymetrix) and infected by incubation for 4 h at 37°C with 156,250 or 2,500 TCID50 (50% tissue culture infectious dose ...
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells were positively enriched by Magnisort Human CD8+T cell positive selection Kit (Invitrogen). Nuclear fraction was isolated using NE-PER Nuclear and Cytoplasmic extraction kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: Allogenic CD8+ were isolated by negative selection using Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen) and labeled with carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Bioengineering 2024Quote: ... CD14+ monocytes were isolated from the suspension using the MagniSortTM Human CD14 Positive Selection Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Molecular Biology 2022Quote: Cell death was determined in adult mouse cardiomyocytes by FITC-conjugated annexin V staining as described previously 31 (Invitrogen Canada Inc., Burlington, ON, Canada). Nuclei were stained by Hoechst 33324 (Invitrogen Canada Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... The araC gene and araBAD promoter were PCR-amplified from pBAD/His/lacZ (Invitrogen, Carlsbad, CA, USA). The lac promoter and inhibitory unit (iUnit ...
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bioRxiv - Molecular Biology 2021Quote: ... A SV40 polyA-PGK promoter-BlasticidinR cDNA-bGH polyA cassette was then synthesized by GeneArt (Thermo Fisher), PCR amplified ...
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bioRxiv - Genomics 2020Quote: Expression cassettes consisting of the doxycycline-inducible cytomegalovirus (CMV) promoter (derived from the Invitrogen T-REx system), the intron-containing EF1α 5 UTR ...
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bioRxiv - Physiology 2020Quote: ... SgRNA elements with the U6 promoter were amplified and subsequently cloned into pAd/PL adenovirus vector (Invitrogen) for virus packaging ...
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bioRxiv - Genetics 2021Quote: Quantitative PCR for promoter regions of interest was performed with Absolute Blue SYBR Green (Thermo Scientific AB4166B) on the CFX96 Real Time System Thermocyclers (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: The sequence of the OpIE2 promoter was obtained from pIB/v5-His map sequence (ThermoFisher Scientific, USA). The truncated version of the wild-type OpIE2 promoter sequence (tIE2) ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: For chrd WISH experiments amplified region was cloned into TOPO II dual promoter vector (Thermo scientific, USA). To generate antisense probe ...
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bioRxiv - Cell Biology 2021Quote: Tissue-specific promoters driving lbp-3 or nlp-11 vectors were generated using Multisite Gateway System (Invitrogen). Promoters were cloned to 1’ position Donor Vector pDONR 221/P1-P4 ...
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bioRxiv - Cell Biology 2023Quote: ... the promoter reporter vector and the pRL-CMV Renilla luciferase plasmid using Lipofectamine 2000 reagent (Invitrogen, USA). After transfection for 24 h ...