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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco, A29133). IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8 T-cells were isolated from PBMCs using the MagniSort Human CD8 Naïve T-cell enrichment kit according to the manufacturer’s instructions (Invitrogen). 1 million naïve CD8+ T-cells were activated with plate-bound anti-CD3 (Biolegend Ultra-LEAF purified ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: Systemic inflammation in plasma was assessed by measuring cytokine levels using commercially available quantitative sandwich ELISA kits for interleukin 1 beta (IL-1β) (Invitrogen, USA, Cat no.:88-7013-88), and tumor necrosis factor α (TNF-α ...
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bioRxiv - Microbiology 2022Quote: ... We extracted the genomic DNA of ELISA-positive cattle using either the magLEAD 12gC or a MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific Inc., Waltham, USA) with an automated nucleic acid extraction system (KingFisher Duo Prime ...
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bioRxiv - Neuroscience 2023Quote: TNFα and IL-6 released in the microglia conditioned medium were quantified using mouse TNFα and IL-6 ELISA kits according to manufacturer’s instructions (Invitrogen, 88-7324 and 88-7064-22, respectively) and a SpectraMax i3x spectrophotometer (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... Tor1A and Tor1B coding regions were amplified from HeLa cDNA and cloned into the vector pcDNA5.1-GFP FRT/TO (cytomegalovirus [CMV] promoter; Invitrogen) for generation of stable ...
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bioRxiv - Cancer Biology 2021Quote: ... and empty pGL3 vector or pGL3 vector containg the DUSP6 promoter regions 191p and 508p using Lipofectamine 2000 (Invitrogen). After 24 hours the Dual-Glo Luciferase Assay System (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... the premiR-PHOT1or premiR-PHOT2 was cloned into the pDONR™ 221 and the SCR promoter into pDONRP4P1r (Invitrogen) vectors which are Gateway® compatible by BP reactions ...
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bioRxiv - Microbiology 2020Quote: ... MDCK-SiaT1 cells were prepared by transfection of the ST6Gal1 gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Cell clones with increased levels of α2,6-linked Sia were identified by staining with the Sambucus nigra (SNA ...
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bioRxiv - Cell Biology 2021Quote: ... Proliferating C2C12 cells were transfected with the myogenin promoter and hnRNPK-expressing plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). The total amount of DNA was kept constant by the addition of the pcDNA3 vector ...
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bioRxiv - Neuroscience 2020Quote: ... we amplified a 1648 bp c-fos promoter fragment by PCR using Phusion High-Fidelity DNA Polymerase (ThermoFisher Scientific) from a previously described plasmid c-Fos-PSD95:Venus-Arc ...
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bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the CMV promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen). The UBR5 RNAi plasmid also encodes EmGFP plus an artificial pre-miRNA targeting mouse UBR5 under bicistronic control of the CMV promoter ...
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bioRxiv - Plant Biology 2020Quote: The plasmid pMDC163 was modified by inserting a 35S CaMV promoter from pDONR207 by using a LR reaction (Invitrogen). The resulting plasmid ...
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bioRxiv - Plant Biology 2022Quote: The GeneART pUC plasmid containing the prOsMYB1R35 promoter sequence was transformed into Escherichia coli DH5α cells (Thermo Fisher Scientific). The promoter was liberated from purified plasmid using BamHI and BglII restriction enzyme digests for the 5’ and 3’ end ...
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bioRxiv - Neuroscience 2021Quote: ... a genomic sequence spanning 150 bp upstream and downstream of the miRNA sequence was PCR-amplified and cloned into a modified pcDNA3.1 with a U6 promoter (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... pENTR-Smarcd1 was combined with pol2 promoter and C-terminal Myc-tag entry clones in a Gateway LR reaction according to the manufacturer’s protocol (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... NFκB-reporter Jurkat cell line expressing cyan fluorescent protein (CFP) under control of NFκB promoter was cultured in RPMI 1640 (Gibco), 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: Genomic regions that were amplified from genomic DNA using PCR or gBlocks were placed upstream of the Ciinte.Fog basal promoter and LacZ using the Gateway technology (Invitrogen) 23,33 ...
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bioRxiv - Plant Biology 2024Quote: ... The HvCLV1 coding region without stop codon (3,573 bp) was amplified from Morex gDNA and inserted downstream of the promoter by Gateway cloning (Invitrogen). A C-terminal mVENUS was integrated downstream of the gateway site by restriction and ligation via PacI and SpeI (Suppl.table1) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then transfected with Renilla and original or promoter-containing pGL4.10 Firefly plasmids in a 1:10 ratio using lipofectamine 3000 (Invitrogen). After 48 h ...
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bioRxiv - Neuroscience 2024Quote: The adcyap1b promoter (Entry Clone) was then recombined into an Gal4FF destination vector (Gateway™ LR recombination, Invitrogen™), derived from the Tol2Kit (Kwan et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... which includes AmpR and gentamicin resistance (GmR) genes and is driven by the AOX1 promoter (Thermo Fisher Scientific, USA). Following the cloning procedure ...
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bioRxiv - Microbiology 2024Quote: ... a DNA fragment containing the promoter region of slr1790 was amplified by PCR and cloned into pJET1.2 (Thermo Scientific). The aadA gene ...
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bioRxiv - Developmental Biology 2024Quote: ... was co-transfected with the empty luciferase reporter vector or the STAT6-promoter luciferase reporter using Lipofectamine3000 (Invitrogen #L3000008) following the manufacturers protocol ...
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bioRxiv - Cell Biology 2021Quote: Primary human vein endothelial cells (HUVECs) were cultured in human endothelial SFM medium (Invitrogen) containing 20% foetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) and cultured in AIMV medium (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA expression analysis was performed with Affymetrix Human ClariomS® microarray using the WT Plus Reagent kit (ThermoFisher Scientific). As starting material ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Biochemistry 2020Quote: Cas9 was produced by IVT using either a continuous exchange 1-Step Human Coupled IVT Kit (Thermo Fisher Scientific) or a PURExpress bacterial IVT kit (New England Biolabs) ...
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bioRxiv - Systems Biology 2020Quote: ... and then cells were fixed and permeated in the plate using a Human Cardiomyocyte Immunocytochemistry Kit (Thermo Fisher Scientific). The primary antibodies included ...
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bioRxiv - Bioengineering 2022Quote: cDNA libraries were constructed using Ion Ampliseq Transcriptome Human Gene Expression Kit from Thermo Fisher (MA, USA, Cat# A26325) according to manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of total RNA was depleted of rRNAs by using the Human/Mouse RiboMinus Transcriptome Isolation Kit (Invitrogen). Ribosomal RNA depletion was confirmed by an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2020Quote: ... and hybridized to the Affymetrix Human Gene 1.0 ST Arrays using the Gene Chip WT Terminal Labeling and Hybridization Kit (Affymetrix). GeneChip fluidics station 450 was used for processing of the arrays and fluorescent signals were detected with the GeneChip scanner 3000-7 G ...
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bioRxiv - Immunology 2021Quote: ... T lymphocytes were purified using the Pan T cell isolation kit (Miltenyi Biotech) and activated with Dynabeads Human T-Activator CD3/CD28 (1:1 beads:cell) (ThermoFisher) in X-vivo 15 medium (Lonza ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA from native pig and human lung tissue (fresh snap-frozen) was isolated using the PureLink Mini Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Anti-human IgM and IgG conjugates provided within the kit were replaced with anti-mouse IgM-HRP-labeled (Invitrogen) and anti-mouse IgG-HRP-labeled (Invitrogen ...
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bioRxiv - Immunology 2020Quote: A CRISPR plasmid targeting human Hk2 was generated using the GeneArt® CRISPR Nuclease Vector Kit from Thermo Fisher. Caco-2 cells were purchased from DSMZ (ACC-169 ...
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bioRxiv - Genomics 2021Quote: ... RNA for gene expression analysis from primary human pseudoislets was extracted using the PicoPure RNA isolation kit (Life Technologies) and cDNA was synthesized using the Maxima first strand cDNA synthesis kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC lines were generated from human peripheral blood CD34+ hematopoietic stem cells (HSC) with a CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... and human CD45+ (hCD45+) bone marrow (BM) cells from transplanted mice using the PureLink Genomic DNA Mini Kit (Invitrogen). DNA was digested using DraI restriction enzyme (NEB ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: RNA extracted from human left ventricular tissue was digested with DNase I (Invitrogen DNA-free DNA removal kit AM1906). 20 ng digested RNA was depleted of rRNA (NEBNext rRNA depletion kit E6350L) ...
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bioRxiv - Molecular Biology 2024Quote: Quantitation of human DNA isolated from blood cells was performed using Qubit 2.0 with the dsDNA High Sensitivity Assay Kit (Thermofisher A custom Ampliseq panel for the detection of SNPs was designed using Ion AmpliSeq Designer and ordered as Ampliseq Custom Panel ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from target K562 cells using the Dynabeads™ Untouched™ Human T Cells Kit (Invitrogen). Surface CAR-positive cells were sorted by flow cytometry ...
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bioRxiv - Cell Biology 2024Quote: A bead-based multiplex immunoassay system (Multiplex kit Cytokine & Chemokine 34-Plex Human ProcartaPlex™ Panel 1A, ThermoFisher, USA) was applied to measure the secretion of cytokines and chemokines by the Silk-Ovarioids samples into the spent culture media after 42 days of culture ...
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bioRxiv - Microbiology 2021Quote: ... Measurements and analysis were performed with the Applied Biosystems™ 7500 Fast Dx Real-Time PCR Systems and associated software (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Bioengineering 2021Quote: ... + 2% human serum albumin (ThermoFisher) + 5 μM ROCKi (Tocris) ...