Labshake search
Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... A 2210-bp SYT3 promoter fragment was cloned into pDONRP4P1R via a BP reaction (Invitrogen) to generate pEN-L4-proSYT3-R1 ...
-
bioRxiv - Microbiology 2021Quote: ... pCP23 multicloning site and Fjoh_0139 promoter after the PstI restriction site was synthesized (Geneart, Thermofisher). A fragment containing lacI open reading frame was PCR amplified using oligonucleotide primers oTM495 and oTM496 ...
-
bioRxiv - Neuroscience 2022Quote: ... in place of the EF1a promoter using Anza 28 MluI and Anza 14 SalI (ThermoFisher). The inverted hM4D(Gi)-mCherry coding sequence in the resulting vector was replaced with inverted human wild-type or NLS1 TDP-43 coding sequences (136 ...
-
bioRxiv - Plant Biology 2023Quote: TOB1 promoter region (2.2 kb) was PCR amplified and cloned into pCR_Blunt vector (Thermo Fisher). This plasmid was used as a template to introduce mutations into putative KNOX1 binding site BS1 to BS3 by inverse PCR ...
-
bioRxiv - Biochemistry 2024Quote: ... or FLAG:ACAD11 expressed under a CMV promoter in pcDNA3.1 constructs using Lipofectamine 3000 (Thermo Scientific) in OptiMEM (Gibco) ...
-
bioRxiv - Immunology 2024Quote: ... Amplified PCR fragments were inserted into the metallothionein promoter driven pMT-V5-His vector (Invitrogen). The generated vector was co-transfected with a pCoBlast blasticidin resistance vector with a ratio of 19:1 and cells were selected in blasticidin containing medium according to manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... 3.1 Kb of DIR13 promoter was PCR-amplified using Phusion DNA Polymerase (Thermo Fisher Scientific) from Col-0 gDNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... driven by an SV40 promoter was mutated using PCR (SuperFI II polymerase Thermo Fisher Scientific) to generate small deletions removing small ELMs from the TDRD3 sequence ...
-
bioRxiv - Plant Biology 2024Quote: ... Amplification of all genes and promoters was performed using Phusion U polymerase (Thermo Fisher Scientific) with primers containing uracil overhangs ...
-
bioRxiv - Cell Biology 2023Quote: ... Percent of cell death was determined by the staining with 5 µM Hoechst 33342 and 2 µM propidium iodide (PI) (Invitrogen, Carlsbas, CA). Images were taken and counted by Zeiss microscope equipped with automated computer-assisted software (Axiovision 4.6 ...
-
bioRxiv - Microbiology 2023Quote: Necrotic cell death was evaluated by staining cells with 500 nM of propidium iodide (PI; Thermo Fisher Scientific, Waltham, MA, USA; P3566). Following a 5-minute staining period ...
-
bioRxiv - Biophysics 2023Quote: ... and treated with 500 ng/ml Doxycycline and directly before the experiment 3 µM SytoxBlue cell death stain (Thermo Fisher Scientific, S34857) is added ...
-
bioRxiv - Immunology 2024Quote: To compare cell death in the presence and absence of calcium cells were stimulated in DMEM or calcium free DMEM (Thermo Fisher Scientific) + 10 μM BAPTA-AM (Tocris).
-
bioRxiv - Immunology 2024Quote: We evaluated the integrity of the neutrophil cell membrane as a metric for cell death using CyQUANT™ lactate dehydrogenase (LDH) cytotoxicity assay (Invitrogen #C20300), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Death cells have been excluded from the analysis using the eBioscience Fixable Viability Dye eFluor780 (1:1000, Thermo Fisher, 65-0865-14). Cells were washed and resuspended in FACS buffer before flow cytometric analysis on a Fortessa X-20 ...
-
bioRxiv - Plant Biology 2024Quote: ... Cell death was quantified as a proportion of green-fluorescing cells by fluorescence imaging (EVOS™ Cy5 and GFP Light Cubes, ThermoFisher Scientific). Growth curves were produced by graphing OD750 as a function of time ...
-
bioRxiv - Microbiology 2020Quote: ... Cell associated immunoglobulin was detected with an appropriate FITC-labelled secondary antibody and fluorescence measured using an Attune NXT cytometer (Thermofisher, UK). Data are shown as relative fluorescence intensities calculated as a percentage of the negative control fluorescence.
-
bioRxiv - Microbiology 2020Quote: Viral contigs were validated experimentally and associated to individual algal sample using Reverse Transcription (SuperScript™ IV reverse transcriptase – Invitrogen™) followed by PCR (Platinum™ SuperFi™ DNA polymerase – Invitrogen™) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Targets of JMML associated Genes were enriched using a custom in-house panel (AmpliSeq JMML panel v2, IAA11909_192; Thermo Fisher Scientific) and processed with the NEBNext Ultra II DNA library preparation kit (New England Biolabs) ...
-
bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
-
bioRxiv - Cell Biology 2023Quote: ... biopsies were co-cultured with 3T3-J2 fibroblasts and Rho-associated protein kinase inhibitor (Y-27632) in epithelial cell expansion medium consisting of a 3:1 ratio DMEM:F12 (Gibco, UK; 21765), 1X penicillin/streptomycin and 5% FBS (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was done on brain sections to assess expression of plasticity associated proteins Nogo-A and BDNF with rabbit anti-Nogo-A (1:200; Invitrogen, USA), rabbit anti-BDNF (1:500 ...
-
bioRxiv - Neuroscience 2024Quote: ... harboring a human AβPP transgene with the K670N and M671L Swedish mutations associated with familial early-onset AD [26] were grown (37°C, 5% CO2) in DMEM/F12 medium (Thermo Scientific) supplemented with 5% FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were analyzed thanks to nano-liquid chromatography (LC) associated to tandem mass spectrometry: an UltiMate 3000 system (NCS-3500RS Nano/Cap System; ThermoFisher Scientific) and an Orbitrap Exploris 480 mass spectrometer supplied with a FAIMS Pro device (ThermoFisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... The resulting lysate was used to quantify the amount of clusterin present in each sample using the Mouse Clusterin ELISA Kit (Thermo Fisher EM18RB) according to manufacturer’s instructions.
-
bioRxiv - Physiology 2021Quote: The concentrations of IL-1β in tissue extracts of ovaries and livers were determined using an IL-1 beta Pro-form Mouse Uncoated ELISA kit (88-8014-22; Thermo Fisher Scientific) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: Supernatants and cell pellets were collected from the interaction between IGRP-GFP CD8+ T-cells and βlox5 cells at a 10:1 ratio and were evaluated using a granzyme B ELISA kit (BMS2027; ThermoFisher Scientific, USA.). The standard curve was produced to determine the granzyme B concentration ...
-
bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
-
bioRxiv - Immunology 2022Quote: ... Mouse lung homogenates were used to determine IgM protein using an IgM uncoated ELISA kit as directed (Invitrogen-ThermoFisher Scientific, Waltham, MA).
-
bioRxiv - Immunology 2022Quote: ... Mouse lung homogenates were used to determine IgM protein using an IgM uncoated ELISA kit as directed (Invitrogen-ThermoFisher Scientific, Waltham, MA).
-
bioRxiv - Immunology 2020Quote: ... The cell supernatant was diluted 10-fold and the TNF-α content was measured with a mouse TNF-α Elisa kit obtained from Invitrogen. The remaining cells were subjected to CCK8 assay to detect cytotoxicity.
-
bioRxiv - Bioengineering 2021Quote: ... The samples were allowed to culture for 12h at 37°C and cytokine concentration was measured in the media by Ready-Set-Go™ ELISA kits (Thermo Fisher) as detailed in the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: The total IgG concentration in mouse serum was determined using the IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, Cat no. 88-50400) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Levels of murine Aβ (1–42) were measured by enzyme-linked immunosorbent assay (ELISA) using commercial kits (Thermo Fisher Scientific, cat# KMB3441) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
-
bioRxiv - Neuroscience 2024Quote: ... The concentration of Aβ1-40 and Aβ1-42 in the s-extracts was determined by commercially available ELISA kits (ThermoFisher Scientific: KHB3481 and KHB3544). Aβ1-42 was undetectable in the Aβ negative sample ...
-
bioRxiv - Immunology 2024Quote: Individual cytokines in supernatants from cell culture experiments were measured using the species-appropriate ELISA Kits mouse IL-6 (Invitrogen™ 88706488), mouse IL-1beta (Invitrogen™ 88701388) ...
-
bioRxiv - Cancer Biology 2022Quote: Levels of phosphorylated proline-rich AKT substrate of 40kDa (phospho-PRAS40 (pT246)) were assessed using PRAS40 ELISA kits (Invitrogen, Camarillo, USA, KHO0421). Cells were lysed in extraction buffer (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22, murine IL-6: Invitrogen 88-7064-88) and a SpectraMax M2 plate reader ...
-
bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... human chimeric B2 were digested with immobilized papain using Pierce™ Fab Preparation kit (ThermoFisher) following the instructions.
-
bioRxiv - Immunology 2022Quote: IL-18 immunoassay was preformed using the ProQuantum Human IL-18 Immunoassay Kit (A35613, Invitrogen) per manufacturer’s protocols with a 1:3 dilution of supernatants collected from primary BEC following indicated infection with S ...
-
bioRxiv - Microbiology 2021Quote: ... IgG yields were quantified using an Easy-Titer Human IgG Assay Kit (ThermoFisher Scientific; 23310). Remaining deidentified plasma samples were aliquoted and stored at -80C.
-
bioRxiv - Immunology 2020Quote: ... through specifically assembled kits MixMatch Human 26 Panel for Luminex MAGPIX detection system (Affymetrix, eBioscience) and following the manufacturers’ instructions ...
-
bioRxiv - Immunology 2021Quote: ... bead-based multiplex kit for Non-Human Primate Cytokines (Cat # EPX370-40045-901, ThermoFisher Scientific) was used according to manufacturer instructions ...
-
bioRxiv - Genetics 2021Quote: ... and qualified via Quantifiler™ Human DNA Quantification Kit (Applied Biosystems, Foster City, CA, USA). All samples were genotyped at the WeGene company (Shenzhen) ...
-
bioRxiv - Microbiology 2020Quote: ... Total RNA pools from human blood infections were globin-depleted using a GLOBINclear kit (Ambion) according to manufacturer’s specifications ...
-
bioRxiv - Immunology 2022Quote: IFN-γ ELISpots were performed using Human IFN gamma ELISpot KIT (Invitrogen, San Diego, CA) and MultiScreen Immobilon-P 96 well filtration plates (EMD Millipore ...
-
bioRxiv - Neuroscience 2024Quote: ... Human IL-1β was measured by IL-1β kit (Thermo Fisher, catalog no. 88-7261). Human Aβ42 was measured by Aβ42 kit (ExCell Bio ...