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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and primers and normalized to β-actin (Hs01060665_g1 ThermoFisher 4331182) using TaqMan Universal Master Mix (ThermoFisher 4304437) ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Thermo Scientific), 100 μg/ml ampicillin and 50 μg/ml kanamycin after 1 day at 30°C ...
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bioRxiv - Biochemistry 2023Quote: ... rabbit anti-β-actin (1:10,000, Life Technologies, MA5-15739), donkey anti-goat IgG HRP-conjugated (1/10,000 ...
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bioRxiv - Genomics 2023Quote: ... and 55 μM β-mercaptoethanol in Knockout D-MEM (Invitrogen) containing 2i (1 μM PD0325901 ...
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bioRxiv - Immunology 2023Quote: ... and 50 mM β-Mercaptoethanol (Gibco, Thermo Fisher Scientific, USA). To induce dendritic cell differentiation ...
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bioRxiv - Immunology 2023Quote: ... and 50 mM β-Mercaptoethanol (Gibco, Thermo Fisher Scientific, USA). To induce dendritic cell differentiation ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by incubation with rabbit-anti β-galactosidase (Invitrogen #A11132) and goat anti-rabbit IgG AlexaFluor488 (Invitrogen #A11008) ...
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bioRxiv - Genomics 2023Quote: ... and β-actin (Invitrogen, cat. no. #MA1-744, 1:5,000) overnight at 4°C with gentle agitation ...
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bioRxiv - Genetics 2022Quote: ... 20mM β-Mercaptoethanol) and protein concentrated by ultrafiltration (Thermo Scientific Pierce Protein Concentrator PES 30K Cat# PI88529S).
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bioRxiv - Cell Biology 2023Quote: Aβ40 and Aβ42 levels were mainly measured by Invitrogen amyloid-β human ELISA Kit (Thermo Fisher, KHB3481 and KHB3441) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and β-actin (1:5000, MAB1501, Chemicon International, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 1mM IPTG (Isopropyl β-d-1- thiogalactopyranoside, Thermo Fisher Scientific) was added to induce gene expression ...
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bioRxiv - Developmental Biology 2023Quote: ... 1x β-Mercaptoethanol (Catalog no. 21-985-023, Fisher Scientific), 3 μM CHIR99021 (Chir ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1x β-mercaptoethanol (Catalog no. 21-985-023, Gibco). For stem cell maintenance N2B27 medium was supplemented with 3 μM CHIR99021 (Chir ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse monoclonal anti-β-actin (1:7500, ThermoFisher, MA1-140) was used as a loading control ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... or goat anti-rabbit Alexa Fluor 488 (Invitrogen, β-actin) diluted in blocking buffer were added and incubated for 1 hour in the dark ...
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bioRxiv - Microbiology 2024Quote: ... fluorescein di-β-D-cellobioside (FDC) purchased from Thermo Fisher Scientific and AAT Bioquest ...
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bioRxiv - Biochemistry 2023Quote: ... rabbit anti-β-Tubulin (Thermo Fisher, 32-2600, 1:2500), rabbit anti-UCP1 (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... with 2.75 mM β-mercaptoethanol (Thermo Fisher Scientific, 21985-023) and RIPA buffer (total volume 10 µL ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-β-Actin antibody (Thermo Fisher Scientific, Waltham, MA) was used as a loading control.
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bioRxiv - Microbiology 2024Quote: ... β-Actin was used (Thermo Fisher Scientific, catalog no. 4331182). The reaction was performed using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... and subsequently cloned into CMV-mCherry-Gateway vector or Vivid Colors™ pcDNA™6.2/N-EmGFP-DEST vector (ThermoFisher Scientific) to obtain CMV-mCherry-DBNL or CMV-EmGFP-DBNL ...
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bioRxiv - Biophysics 2019Quote: ... N-terminal-tagged MBP-TRPA1 under the control of a CMV promoter was expressed in HEK293F cells using the BacMam system (Thermo Fisher). Protein expression was induced at cell density 1.5-2.5 × 106 cells ml- with media supplemented with 5 mM sodium butyrate and 5 µM ruthenium red ...
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bioRxiv - Microbiology 2020Quote: ORFs were cloned into the pENTR1A construct and transferred into the pLENTI CMV puro or blast vectors using a Gateway enzyme mix (Invitrogen #56484). Lentivirus was produced by co-transfection of the pLenti CMV plasmids with pMD2.G and psPAX2 plasmids ...
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bioRxiv - Neuroscience 2019Quote: ... 1.5 ug of CMV-Egr3 vector or CMV vector was co-transfected with 1 ug of each promoter reporter vector per well using Lipofectamine 3000 reagents (ThermoFisher Scientific). Transfection was performed with 3.75 μl of Lipofectamine 3000 reagent ...
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bioRxiv - Microbiology 2020Quote: ... 3.6 µg pSp-Cas9(BB)-2A-GFP-sgRNA and 400 ng pSc-TIA- CMV-BSR-TIA were mixed with polyethylenimine and OptiMEM (#51985026, ThermoFisher Scientific) for transfection of cells ...
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bioRxiv - Systems Biology 2020Quote: 5637 cells were placed in 24 wells plate and transfected with the lentiviral vectors pTSB-CMV-puro and SiRNA against ACSS1 reaching 70%-80% confluence using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... pCMV-EML4-ALK(K589M)-P2A-H2B-iRFP and CMV-3XFLAG-mCh-EML4-ALK were transiently transfected into wt Beas2B or Beas2B mNG:Grb2 cells using Lipofectamine™ 3000 (Invitrogen, L3000001). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... an shRNA-generating sequence equivalent to the above siRNA sequence was inserted into the pSilencer 4.1 CMV-Hygro vector (Ambion, Austin TX). The plasmid was transfected into Neuro2a cells and clonal lines selected by incubation with Hygromycin (300 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... was provided by Allele Biotechnology and Pharmaceuticals (Shaner et al. 2013)) under constitutively active CMV promoter was transfected to the seeded cells using Lipofectamine 3000 (Invitrogen, L3000001) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... They were individually transferred into either bait pB-CMV-CVn-neo (for PLK2) or prey pB-CMV-YFP-CC-puro (for RB1, TUBB, CHK1, PLK1) vectors using Gateway recombination reaction (Life Technologies) (76) ...
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bioRxiv - Biochemistry 2020Quote: ... containing 300 µg of PB-CMV plasmid DNA was mixed with 15 mL Opti-MEM medium containing 400 µL 293fectin reagent (Thermo Fisher). The mixture was incubated for 5 minutes before being added to the shaker flask ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA of N-terminal Flag-tagged wild-type human eIF4A1 was amplified by PCR and subcloned into the pLenti-CMV-Puro-DEST (w118-1) vector 90 using the Gateway cloning system (Thermo Fisher). The TIMMDC1 ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
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bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the cytomegalovirus (CMV) promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen, Carlsbad, CA). The Fbxl22 RNAi plasmid encodes EmGFP and an artificial pre-miRNA targeting the full length gene of mouse Fbxl22 under bicistronic control of the CMV promoter ...
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bioRxiv - Immunology 2021Quote: ... and firefly Luc reporter gene plasmid (pHR′ CMV Luc) (47) in a 1:17:17 ratio in Opti-MEM (Life Technologies). Transfection mixtures were added to pre-seeded HEK 293T/17 cells in T-75 flasks containing 12 ml of growth medium and incubated for 16-20 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: A construct encoding for CDC-20::3xFLAG under a CMV promoter was transfected into Freestyle 293-F cells (Thermo Fisher Scientific). 48 hours later ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transiently co-transfected with individual sgRNA cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA along with pCMV-sadCas9-VP64 for 48 hours using Opti-MEM Reduced Serum Medium (Thermo Fisher) and X-tremeGENE HP (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Co-transfection of CMV-GFP construct and plasmids packaged with pCMV-vsvg and pCMV-gp was achieved using lipofectamine 3000 (Life Technologies). The medium of transfected cells was collected and concentrated by ultracentrifugation (20,000 for two hours at 4°C ...
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bioRxiv - Physiology 2023Quote: ... as previously described.23 The cDNA of mitAT1.03 was ligated and cloned into the SalI/NotI sites of a pAd-CMV-V5-DEST Gateway Vector (Thermo Fisher Scientific), and the adenovirus vector containing mitAT1.03 cDNA (Ad-mitAT1.03 ...
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bioRxiv - Molecular Biology 2023Quote: ... For the overexpression of SERPINB2, pLENTI-GII-CMV-GFP-2A-Puro (abm, #434730610395) was transfected in fibroblasts using Lipofectamine 2000 (Invitrogen, #11668019).
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bioRxiv - Developmental Biology 2023Quote: ... The gene block was integrated into a plasmid containing a CMV promoter using pcDNA3.3-TOPO TA cloning kit (Invitrogen, K8300-01) in accordance with manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were co-transfected with 100 ng of each target plasmid along with 100 ng of pGL3-ISRE-Luc and 20 ng of pRL-CMV using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000015). Cells were lysed 24 h post-transfection using the Dual-Glo Luciferase Assay System (Promega ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... elegans BUB-1 TPR (1-189)::3xFLAG under a CMV promoter was transfected into Freestyle 293-F cells (Thermo Fisher Scientific). 48 hours later ...
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bioRxiv - Microbiology 2020Quote: ... The appropriate amounts of HILIC particle in 80% ACN were placed in Pierce spin columns (Thermo Scientific) and equilibrated three times using 80% ACN ...
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bioRxiv - Molecular Biology 2022Quote: ... Data acquisition was performed with EPU Automated Data Acquisition Software for Single Particle Analysis (Thermo Fisher Scientific) at -0.3 μm to -3.5 μm defocus.
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bioRxiv - Bioengineering 2021Quote: ... streptavidin-functionalized particles (Life Technologies (Dynabeads MyOne™ Streptavidin C1, Cat. # 65001, Life Technologies, Carlsbad, CA, USA) were coated with biotinylated antibodies for CTXII (cat ...
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bioRxiv - Biochemistry 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Scientific) equipped with a Dionex IonPac AS11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were injected onto a Hypercarb column (3 μm particle size, 4.6 × 100 mm, Thermo Fisher Scientific) at a flow rate of 0.6 mL/min on an ACQUITY UPLC System ...