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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 7H Cyclopenta c pyridin 7 one 5 6 dihydro 3 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Molecular Biology 2020Quote: ... 3) and four tyr (clutch no. 7) mutants using the Invitrogen TOPO TA kit according to the manufactures protocol (Invitrogen catalogue no. K4575J10), and Sanger sequenced the extracted plasmids from 6-10 colonies per embryo (Fig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: The number of epithelial cells in the Cervix Chip was obtained by digesting the Cervix Chip apical channel at day 7 of differentiation or day 3 on co-culture with bacteria with Collagenase IV (Gibco, Cat. No. 17104019) in TrypLE (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Microbiology 2021Quote: Bone marrow derived macrophages (BMDMs) were generated from 6-8 week old BALB/c mice in RPMI-1640 (Gibco) containing 10% foetal calf serum ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were incubated at 37°C for 30 min and loaded onto 6% DNA retardation gels (Thermo Fisher Scientific). DNA was detected by staining the gel with GelRed (VWR) ...
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bioRxiv - Microbiology 2022Quote: ... 60 °C for 1 min) X 49 cycles on the QuantStudio 6 flex Real-time PCR system (Applied Biosystems), using the TaqMan RNA-to-CT 1-step kit (Applied Biosystems).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... adult WT C57BL/6J mouse testes were dissected and incubated at 37 °C for 6 h in either Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, Thermo Scientific) or compound 5 (100 μM ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...
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bioRxiv - Neuroscience 2021Quote: ... Fly brains were incubated with 100 nM Tetramethylrhodamine methyl ester (TMRM) (Invitrogen, I34361) for 30 min at 37 °C and washed three times with Hank’s balanced salt solution (HBSS) ...
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bioRxiv - Neuroscience 2019Quote: ... The co-cultures were stained with tetramethylrhodamine methyl ester (TMRM, Invitrogen; 250 nM) for 45 min at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were loaded with 10 nM Tetramethyl Rhodamine Methyl Ester (TMRM) (Molecular Probes) and incubated at 37°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... and the mitochondria-specific dye tetramethylrhodamine methyl ester (TMRM, 25nM; Thermo Fisher Scientific) diluted in Hanks solution (Gibco ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Cells were incubated with 15 nM tetramethyl rhodamine methyl ester (TMRM; #T668; Invitrogen) in culture medium for 25 min at 37°C and 5% CO2 in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... FM4-64 and leucyl-L-leucine methyl ester (LLOMe) were from Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... and subsequently alkylated with 50 mM S-methyl methanethiosulfonate (MMTS) (Thermo Fisher Scientific) in USS for 1 h at room temperature ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... N-methyl-D-aspartate receptor 1 (NMDAR1, rabbit, 1:1000, Invitrogen PA5-34599), N-methyl-D-aspartate receptor 2B (NMDAR2B ...
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bioRxiv - Cell Biology 2022Quote: ... an equivalent of 1 μg total protein starting material was injected using the μL-pickup method with 0.1% v/v formic acid as a transport liquid from a cooled autosampler (5 °C) and loaded onto a trap column (μPrecolumn cartridge, Acclaim PepMap100 C18, 5 μm, 100 Å, 300 μmx5 mm, Thermo Scientific). Peptides were separated on the nano-LC column using a linear gradient from 2-45% v/v acetonitrile plus 0.1% v/v formic acid in 85 min at a flowrate of 300 nL/min ...
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bioRxiv - Microbiology 2019Quote: ... Strains were routinely grown at 37°C and 5% CO2 overnight on tryptic soy agar plates supplemented with 5% sheep blood (TSAII) (Thermo Fisher Scientific). Growth in broth culture was performed in BHI (Becton ...
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bioRxiv - Molecular Biology 2021Quote: ... After 5 h incubation at 37°C with 5% CO2 cell were washed twice with PBS containing proteases inhibitors (Thermo Fisher Scientific), spun down at 1000 g for 5 min at 4°C and snap frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubating at 37°C in a 5% CO2 humidified incubator in DFGM supplemented with 5 ng mL−1 TGF-β1 (Thermo Fisher Scientific) and 100 μg mL−1 ascorbic acid ...
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bioRxiv - Cell Biology 2023Quote: ... an equivalent of 1 µg total protein starting material was injected using the µL-pickup method with 0.1% v/v formic acid as a transport liquid from a cooled autosampler (5 °C) and loaded onto a trap column (µPrecolumn cartridge, Acclaim PepMap100 C18, 5 µm, 100 Å, 300 µmx5 mm, Thermo Scientific). Peptides were separated on the nano-LC column using a linear gradient from 2-30% v/v acetonitrile plus 0.1% v/v formic acid in 75 min at a flow rate of 300 nL/min ...
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bioRxiv - Genomics 2024Quote: Human dermal fibroblasts (ATCC PCS-201-010) were maintained at 37°C with 5% CO2 and 5% O2 using low glucose DMEM (ThermoFisher 11885-084) enriched with 15% FBS (GenClone 25-550 ...
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bioRxiv - Genomics 2019Quote: ... Samples were heat-denatured (95°C/ 5 min) and incubated o/n at 37°C with 150 U of λ-exonuclease (λ-exo; Thermo Scientific) in the presence of 40 U of RNAseOUT ...
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bioRxiv - Microbiology 2019Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 20 s and was run on QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). Provided that the efficiencies of the reactions were between 90% and 110% ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Molecular Biology 2022Quote: ... The grid was blotted for 7 sec at 4°C with 100% humidity and vitrified using a Vitrobot Mark IV (Thermo Fisher Scientific, USA) by plunging into the liquid ethane cooled by liquid nitrogen.
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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bioRxiv - Genomics 2022Quote: ... HEK293FT cells were replated at a 1:3 dilution one day post-transfection into media supplemented with 1 μg/mL final concentration puromycin (Thermo Fisher Scientific). HEPG2 cells (American Type Culture Collection (ATCC – HB8065 ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293FT cells were replated at a 1:3 dilution one day post-transfection into media supplemented with 1 µg/mL final concentration puromycin (Thermo Fisher Scientific). HEPG2 cells (American Type Culture Collection (ATCC – HB8065 ...
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bioRxiv - Synthetic Biology 2022Quote: ... situated between nsP4 and the viral 3’ untranslated region (UTR) were reverse-transcribed and PCR-amplified using the SuperScript™ IV One-Step RT-PCR System (ThermoFisher, #12594025) and the 26S-F primer and pooled SinRev primer ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 × 105 cells were seeded in one well of a 12-well plate on day 0 and counted on day one until day 3 with cell counters (Thermo Fisher Scientific). For colony formation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... One million live cells were plated in 5 ml complete Neurobasal culture medium (Neurobasal™ Medium (Gibco, cat. no. 21103049), 1x B-27 supplement (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... 5×105 MDCK II cells were seeded into 24 mm transwells one day before transfection using Lipofectamine 3000 Reagent (Invitrogen). 6 h post transfection ...
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bioRxiv - Plant Biology 2020Quote: Regions screened for transcription factor binding via Yeast One-Hybrid were first inserted into pENTR 5’TOPO TA entry vector (Thermofisher) and subsequently placed into the pMW2 and pMW3 destination vectors containing HIS3 and LACZ marker genes respectively (Deplancke et al. ...
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β-importins Tnpo-SR and Cadmus and the small GTPase Ran promote ovarian cyst formation in DrosophilabioRxiv - Developmental Biology 2021Quote: ... dissected ovaries were incubated for one hour at room temperature in Grace's media containing 10 μM 5-ethynyl-2′-deoxyuridine (EdU; Life Technologies). The following primary antibodies were diluted in blocking solution and used overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were isolated with NP40 lysis buffer with a 5 min incubation on ice and passed through a 70 μm cell strainer (Greiner Bio-One EASYstrainer, ThermoFisher). Samples were pooled based on nuclei concentration (Countess II cell counter ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for one hour with 5% normal donkey serum (NDS) in TKBPS (KBPS with 0.25% Triton X-100 (Fisher Scientific)) ...
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bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
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bioRxiv - Genetics 2020Quote: ... RNA was quantified using a NanoDrop™ One (#ND-ONE-W, ThermoFisher Scientific) and 2 μg per sample were used for cDNA synthesis by performing a retro-transcription reaction with the SuperScript™ VILO™ cDNA Synthesis Kit (#11754050 ...
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bioRxiv - Microbiology 2021Quote: ... as measured by NanoDrop One/One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific) was loaded onto a 50-cm μPAC capLC column (PharmaFluidics ...
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bioRxiv - Microbiology 2022Quote: NanoDrop One Microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific, catalog #: ND-ONE-W).