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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 7H Cyclopenta c pyridin 7 one 5 6 dihydro 3 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... at 4°C for 3 hours followed by addition of 10 ml Protein A Dynabeads (Invitrogen) for 20 min to capture the antibody-protein complexes ...
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bioRxiv - Bioengineering 2023Quote: ... KM-met released from CS-NP was quantified at 233 nm using a NanoDrop One microvolume UV-Vis spectrophotometer for up to 6 hours at room temperature (Thermofisher Scientific, Waltham, MA, USA). At the endpoint ...
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bioRxiv - Microbiology 2019Quote: ... 5 to 6 seeds were pipetted into 120 x 120 mm square petri dishes (Thermo Fisher Scientific) containing half-strength Murashige and Skoog salts (MS) ...
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bioRxiv - Cancer Biology 2022Quote: ... real-time quantitative PCR was done using the ABI Quant Studio 5 and 6 (Life technologies, USA). The cDNA was used as template and DyNAmo Flash SYBR Green qPCR kit (#F-416L ...
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bioRxiv - Cell Biology 2022Quote: ... about ∼5 μg of bacmid were transfected using 6 μl of Cellfectin II reagent (Thermo Fisher Scientific). 5 days after initial transfection ...
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bioRxiv - Immunology 2021Quote: ... Sorted memory CD4+ T cells were labelled with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, ThermoFisher) and cultured at a ratio of 2:1 with irradiated autologous monocytes untreated or pulsed for 3 h with recombinant SARS-CoV-2 Spike protein (2.5 μg/ml) ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection with 5 ug total DNA in a 6 well plate was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Cell Biology 2023Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole or TO-PRO-3 (Thermo Fisher Scientific) staining.
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Microbiology 2023Quote: ... A NanoDrop One (ThermoFisher) was used to measure the RNA concentration and purity ...
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bioRxiv - Genomics 2023Quote: ... Nanodrop One (Fisher Scientific) for purity ...
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bioRxiv - Microbiology 2021Quote: ... A recombinant lentivirus vector expressing the coding sequence of the EBV transactivator BZLF1 under control of a tetracycline-regulated promoter was constructed by cloning the open reading frame amplified with the primers 5’-CGACCGGTATGATGGACCCAAACTCGAC-3’ and 5’-CGACGCGTTTAGAAATTTAA GAGATCCTCGTGT-3’ into the Age I and Mlu I sites of the pTRIPZ lentiviral vector (Thermo Fisher Scientific, USA). For virus production ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection of MCF-7 and MCF-7/ADR cells was performed with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Hematopoietic precursor cell-7 (HPC-7) cells were grown in IMDM medium (12440-053, Invitrogen) with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... and single cell suspensions stained with 7-aminoactinomycin D (7-AAD; ThermoFisher Scientific, Waltham, Massachusetts) were analyzed on an LSR II flow cytometer (Becton Dickinson).
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bioRxiv - Immunology 2020Quote: ... Cell death was assessed by 7-aminoactinomycin D (7-AAD, Affymetrix eBioscience, San Diego, CA) staining and flow-cytometry (FACSCantoII™ ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 as well as the flanking 5’ and 3’ nuclear localizing sequences and 5’-V5 tag from the GeneArt CRISPR Nuclease Vector (Thermo Fisher Scientific). HEK293-Cas9 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Cell Biology 2023Quote: ... All drug treatments were performed for 3 – 5 hours prior to imaging with a final concentration of 5 nM of actinomycin D (Gibco, 11805-017) or 1 µM of BMH-21 (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2020Quote: ... and then 40 cycles of 95°C for 1 s and 60°C for 30 s in QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). PCR primers were designed using the Primer-BLAST tool available from the NCBI web site (57) ...
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bioRxiv - Microbiology 2021Quote: ... then 40 cycles of 94°C for 1 minute and 60°C for 1 minute on a QuantStudio 3 real-time thermocycler (Thermo Fisher Scientific, Waltham, MA, USA). KPPR1 genomic DNA was used as positive control and 100% reference for calculating Klebsiella relative abundances ...
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bioRxiv - Immunology 2022Quote: 90μL of paraformaldehyde fixed cells was incubated with 5 x 10-7 M DAPI and 1:100 Vybrant™ DiD Cell-Labeling Solution (Invitrogen) at 37C for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Neurospheres were passaged by centrifuging 5 min at 1,000 rpm and incubating for 7 min in Accutase (Gibco® A11105-01) before mechanically dissociating the spheres into single-cell suspension.
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bioRxiv - Immunology 2021Quote: ... a T-cell proliferation assay was performed by incubating collected mice spleen cells for 7 min at RT with 5 μM Carboxyfluorescein succinimidyl ester (CFSE) (Life Technologies), staining was quenched with FCS and splenocytes cultured at 106 cells/well in 96-well plates for 5 days at 37°C in 5% CO2 with 3 μg/ml of rSp antigen ...
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bioRxiv - Immunology 2022Quote: 22-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-23,24-Bisnor-5-Cholen-3β-Ol-cholesterol (22-NBD-cholesterol) (N1148) and 7-NBD-PE (N360) were obtained from Thermo Fisher and dissolved in ethanol at 1 mM ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was performed by standard TaqMan Assay on either the QuantStudio 7 or Quantstudio 5 Real-Time PCR platform (Applied Biosystems). Briefly ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The medium was changed every day and cells were passaged at 70%-80% confluency every 5-7 days using PBSCa-/Mg- (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was performed with 2.5 ng of DNA in triplicates using PowerSYBR Green PCR master mix at Quant Studio 7 qPCR machine (both Applied Biosystems) according to the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...