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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... anti-integrin β1 antibody and 4’,6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was then visualised using 1 µg ml-1 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) or 4 µM TO-PRO-3 Iodide (TOPRO ...
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bioRxiv - Cell Biology 2022Quote: ... typically 2-20 μg samples were loaded on 4–12% Bis-tris gradient gels (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Coverslips were mounted with ProLong Gold antifade plus 4’,6-diamidino-2-phenylinodole (DAPI; Molecular Probes). Images were acquired on a DeltaVision Elite microscope using a 60X ...
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bioRxiv - Physiology 2023Quote: ... isolated FDB fibers were loaded with 4 µM fura-2 AM (Thermo Fisher, Carlsbad, CA, USA) in Ringer’s solution at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were counterstained with 0.3 µM 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies, OR, USA) for 15 minutes before rinsing with cold phosphate-buffered saline pH = 7.5 (Panum Institute Substrate Department ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants were first precleared for 2 h at 4°C using Protein G Dynabeads (Invitrogen). Precleared samples were immunoprecipitated with anti-H3K9me3 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4 mL trituration buffer (Hibernate-A with 2% B27; ThermoFisher Scientific) and 2 mM sodium pyruvate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in PBS containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, #D1306) and analyzed with CytoFLEX Flow Cytometer (Beckman Coulter).
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bioRxiv - Evolutionary Biology 2023Quote: ... Embryos older than 4 days were treated with 2 u/µl T1 RNAse (Thermo Fisher Scientific) after probe washing in order to reduce background ...
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bioRxiv - Bioengineering 2023Quote: ... Hoechst 33342 trihydrochloride trihydrate and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... fetal bovine serum (FBS) and 4’,6-diamidino-2- phenylindole (DAPI) were purchased from Life Technologies. Antibiotics (penicillin/streptomycin ...
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bioRxiv - Biophysics 2023Quote: ... covered with sulfosuccinimidyl 6(4-azido-2-nitrophenyl-amino) hexanoate (sulfo-SANPAH) (ThermoFisher Scientific, Loughborough, UK) 0.5 mg/mL in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... counterstaining was performed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, Thermo Fisher Scientific, Cat# D1306) (1μg/ml ...
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bioRxiv - Biophysics 2024Quote: ... Gel surfaces were functionalized with sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH, ThermoFisher 22589) and coated overnight with collagen-I at 4 °C to ensure cell attachment ...
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bioRxiv - Bioengineering 2024Quote: ... Cell nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI; D1306, Invitrogen, Thermo Fisher Scientific). Stained organoid unit sections were observed with optical sectioning fluorescence microscopy (Axio Observer 7 with Apotome 3 ...
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bioRxiv - Bioengineering 2024Quote: ... Cell nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI; D1306, Invitrogen, Thermo Fisher Scientific). Stained organoid unit sections were observed with optical sectioning fluorescence microscopy (Axio Observer 7 with Apotome 3 ...
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bioRxiv - Immunology 2024Quote: ... These were transfected at a 1:2:4 w/w ratio using Lipofectamine 3000 (Invitrogen #L3000001), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysate was incubated at 4°C for 2 hours with Dynabeads Protein G (Invitrogen, 10003D) magnetic beads that bound anti-RNA polymerase II C-terminal domain antibody CTD4H8 (Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... coverslips were mounted with ProLong Gold antifade plus 4’,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Images were captured using a DeltaVision Elite microscope equipped with a 60X ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were counterstained with the nuclear marker 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen Cat# D1306) at a dilution of 1:1000 in diluent (Agilent Cat# S0809 ...
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bioRxiv - Microbiology 2024Quote: We quantified round 2 PCR product concentration using a Qubit 4 Fluorometer (ThermoFisher, Ref. No. Q33238). We then pooled samples at masses (ng ...
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bioRxiv - Physiology 2024Quote: ... for 20 min at 4°C in PBS containing 2% fetal bovine serum (FBS) (10270, Gibco). Cells were then labeled with CD45 (25-0451-81 ...
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bioRxiv - Neuroscience 2024Quote: ... nuclei were visualized with DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 0.2 μg/ml; Life Technologies). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI: Thermo Fisher Scientific Cat D1306). The use of P2Y13 ...
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bioRxiv - Genomics 2024Quote: ... and lacking sodium pyruvate and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (McCoy‘s 5A, Gibco 16600082), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2024Quote: ... cell viability was measured using 4′,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific, Catalog No. 62248) where only dead cell nuclei would be labelled ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Cas. No. 28718-90-3, Thermo Scientific GmbH, Germany), Tween 20 (Art ...
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bioRxiv - Cell Biology 2024Quote: ... ProLong Gold Diamond 4′,6-diamidino-2-phenylindole (DAPI) mounting media was purchased from Invitrogen (#P36962). Trpv4 antagonist GSK2193874 (GSK219 ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 mL HisTrap column was washed with 10 column volumes of wash buffer (2× GIBCO 14200-075 PBS, 5 mM Imidazole, pH 7.5) followed by 6 column volumes of elution buffer (2× GIBCO 14200-075 PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 300 μm × 5 mm, 5 μm, 100 Å, separation column: C18, Acclaim PepMap, 75 μm × 500 mm, 2 μm, 100 Å, Thermo Fisher Scientific). After loading the sample on the pre-column ...
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bioRxiv - Immunology 2020Quote: ... 25 μl/well of 60 mM water solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Thermo Fisher Scientific) were added ...
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bioRxiv - Neuroscience 2023Quote: ... The carboxylic groups on the carbon fiber surface were electro-activated by incubation in 0.4 M 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC; Life Technologies) and 0.1 M and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Physiology 2022Quote: ... The mitochondria membrane potential was also assessed in cells stained with 50 nM tetramethyl rhodamine ethyl ester (TMRE, ThermoFisher, T669) and subsequently collected in HBSS containing 5 mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... y229Gt (Tg(tnks1bp1:EGFP)) transgenic larvae were exposed to 250 nM tetramethylrhodamine ethyl ester perchlorate (TMRE, Thermo Fisher, Cat# T669) diluted in EM for 25 minutes while wrapped in foil ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of ion-pairing agent (0.1% o-toluidine blue, Carolina Biological) and 3 µL of ethyl acetate (ACS grade, Fisher Scientific). The mixture was vortexed and centrifuged briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... For EU pulse labelling and Click-iT reaction cells were grown in the same conditions as for the immunostaining but pulsed with 50 mM ethyl-uridine (Thermofisher) (EU ...
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bioRxiv - Cell Biology 2021Quote: Mitochondrial membrane potential was tested using the fluorescent potentiometric compound tetramethylrhodamine methyl ester (TMRM, 20 nM; Invitrogen). Cells were incubated in DMEM without phenol red with 0.1% FBS and CsH (1 µM ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA plus PIC) with 20 mM thiol-reactive methyl-methane thiosulfonate (MMTS, Thermo Fisher Scientific) to block free cysteine residues ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 μl of N-methyl-N-trimethylsilyl trifluoroacetamide (MSTFA, Pierce) with 1% trimethylchlorosilane (1% TMCS, Thermo Scientific) was added to react for 30 min in 60°C ...
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bioRxiv - Cancer Biology 2022Quote: ... then disaggregated and labelled with 1,1’-di-octa-decyl-3,3,3’,3’-tetra-methyl-indo-carbo-cya-nine perchlorate (DiI, ThermoFisher) and finally resuspended in a buffer containing 5% FBS in PBS ...
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bioRxiv - Physiology 2024Quote: ... samples were incubated with methyl methanethiosulfonate (MMTS, 1 mol/L, Cat. # 23011; Thermo Fisher Scientific, Dreieich, Germany) at 50°C for 30 minutes to block free thiols ...
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bioRxiv - Microbiology 2024Quote: ... then PEGylated in situ using the methyl-PEG-NHS-ester reagent MS(PEG)12 (#22685, ThermoFisher Scientific). PEGylation was performed to reduce preferred orientation ...
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bioRxiv - Microbiology 2024Quote: ... The extracted fatty acid methyl esters were analysed using gas chromatography (Trace GC Ultra, Thermo Scientific, Germany) coupled to a mass spectrometer (ISQ ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected and 48 later incubated for 40min with 100nM tetramethylrhodamine methyl ester (TMRM) (#T668, Thermofisher), a fluorescent lipophilic cation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Infected cell pellets were centrifuged at 5000xg for 5 min at 4 °C and resuspended in Trizol (Ambion).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then stained with 5 µL FITC anti-human DR4 (Thermo Fisher Scientific, Clone DR-4-02) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...