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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Apoptosis was evaluated thanks to 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, USA) and Annexin-V-APC (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:3000; #D3571, Invitrogen) then mounted on glass slides with Mowiol ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI) for nuclear staining (H3570; Invitrogen; dilution 1:200), diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear counterstaining was performed with 100 ng/ml DAPI (4’,6-diamidino-2-phenylindole; Invitrogen) solution in PBS for 20 min ...
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bioRxiv - Biophysics 2024Quote: ... to visualize actin filaments and with 4’-6-diamidino-2-phenylindole (DAPI, Invitrogen, 1:200) for the nucleus ...
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bioRxiv - Cell Biology 2024Quote: ... mice were injected intraperitoneally with 4-ethynyl-2’-deoxyuridine (EdU; E10187; Invitrogen, Waltham, MA, USA) at 10mg/kg to assay cellular proliferation ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected retinas were either fixed in 4% formaldehyde (MilliporeSigma, Cat.# FX0415-4) in PBS for 2 hours at room temperature or lysed with RIPA buffer (Thermo Fisher Scientific, Cat.# 89900) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... cell pellets were resuspended into PBS with 2% FBS and 1µg/ml of 4’,6-diamidino-2-phenylindol (DAPI) (Thermo Fisher Scientific, Cat#D1306) and transferred into 5 ml round bottom polystyrene flow tubes with cell strainer (Corning Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were cut into 2-4 mm3 pieces and resuspended in RPMI 1640 media supplemented with 2% FBS (Thermo Scientific, Waltham, MA, USA). These pieces then underwent mechanical and enzymatic digestion for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were grown exponentially in an incubator at 37°C under 5% CO2 and subcultured at ratios of 1:2-1:10 every 2 to 4 days using 0.05% trypsin-EDTA (Thermo Fisher Scientific, Cat# 25300120).
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from BoDV-2-infected Vero cells was ligated with either 5’ adaptor oligoRNA (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA (Thermo Fisher Scientific, USA; #L150201)) or 3’ adaptor oligoRNA (5’-GAAGAGAAGGUGGAAAUGGCGUUUUGG ...
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bioRxiv - Biochemistry 2020Quote: In vivo Δψm was measured using the cell-permeant red-fluorescent dye TMRE (tetramethylrhodamine ethyl ester, Thermo Fisher Scientific). For each time point ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
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Loss of TMEM55B modulates lipid metabolism through dysregulated lipophagy and mitochondrial functionbioRxiv - Cell Biology 2024Quote: ... HepG2 cells were harvested with 0.05% Trypsin-EDTA and resuspended in PBS with 50 nM tetramethylrhodamine ethyl ester (TMRE, T669, Invitrogen) for 25 minutes at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with 60 nM of the potentiometric dye tetramethylrhodamine methyl ester perchlorate (TMRM) (Invitrogen), 30 nM MitoTracker™ Green (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250 ...
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bioRxiv - Cell Biology 2022Quote: ... Δψm in hPASMC was measured using the fluorescent probe tetramethylrhodamine methyl ester (TMRM, ThermoFisher Scientific, T7668), following the staining method described by Creed and McKenzie68 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed twice and loaded with 25 nM tetramethylrhodamine methyl ester (TMRM, Thermo Fisher Scientific), a lipophilic cationic dye that accumulates within mitochondria in inverse proportion to Δψm according to the Nernst equation ...
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bioRxiv - Molecular Biology 2020Quote: Mitochondrial membrane potential was evaluated with a flow cytometer and Tetramethylrhodamine Methyl Ester (TMRM, Invitrogen™) stain ...
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bioRxiv - Synthetic Biology 2020Quote: Quantification of methyl ketones was performed on a Trace GC Ultra (Thermo Scientific, Waltham, MA, USA) equipped with a flame ionization detector (FID) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with 3X cholesterol or 5mM methyl beta cyclodextrin (MßC) (#377110050, Thermo Fisher) (doses chosen for biological effectiveness but minimal cellular toxicity [Supplementary Figures 2 and 3] ...
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bioRxiv - Genetics 2024Quote: ... Oligonucleotides for gene promoters were designed in Methyl Primer Express™ Software v1.0 (Applied Biosystems, USA), following directions in Wojdacz et al ...
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bioRxiv - Neuroscience 2023Quote: ... Tetramethylrhodamine methyl ester (TMRM, #2321830), horseradish peroxidase (HRP, #9003990) and AmplexUltra-Red (#2365697) were from Invitrogen Thermo Fisher Scientific (OR ...
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bioRxiv - Neuroscience 2024Quote: ... 100 nM tetramethylrhodamine methyl ester perchlorate (TMRM) combined with 100 nM MitoTracker Green (both from Invitrogen) was used ...
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks and nasal washes were diluted 10-fold in 1× minimum essential medium (MEM) (10% 10× minimal essential medium [Gibco], 2 mM L-glutamine, 0.1% of sodium bicarbonate [wt/vol; Gibco], 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovaries from ∼2-5 flies were dissected in Schneider’s Drosophila media (ThermoFisher SKU #21720-001) that was also used for ex vivo culturing ...
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and loaded with 5 μM Rhod-2 AM (R1244, Invitrogen) in HBSS without calcium/magnesium for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached after 2 or 5 days of co-culture with StemPro Accutase (Thermofisher), experimental duplicates were performed for each timepoint and each condition ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Trimer was reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Thermo Scientific) and alkylated with 10 mM 2-chloroacetamide (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... an EdU (5-ethynyl-2′-deoxyuridine) proliferation assay was performed as recommended by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Fibers were incubated in 5 µM rhod-2 in the membrane permeable AM form (Invitrogen). Rhod-2 was excited with 531 nm light and the emitted light was collected through a 585 nm long-pass filter.
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bioRxiv - Molecular Biology 2022Quote: ... 5 x 107 ESCs were fixed with 2 mM DSG (disuccinimidyl glutarate, Thermo Fisher Scientific) for 50 min at 25⁰C and then 1% formaldehyde (methanol-free ...
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bioRxiv - Microbiology 2022Quote: Larvae were immersed in 100 μg/mL 5-ethynyl-2′-deoxyuridine (EdU) solution (A10044; Invitrogen) for 16 h before termination of the experiment ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were counterstained by Hoechst 33342 (Thermo Fisher Scientific, 2 μg/mL, 5 min, RT), and slides were mounted in ProLong Gold antifade mountant (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of proteins was achieved with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and samples were subsequently alkylated in 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, E10187) before and after the session ...
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bioRxiv - Neuroscience 2022Quote: Mice were injected with 100 mg/kg of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen #E10187) by intraperitoneal injection for 5 consecutive days ...