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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... RNA quantity and quality were determined with a NanoDrop OneC Spectrophotometer (Thermo Fisher), one-step RT-qPCR was performed using a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μg of protein was resolved on 4-12% Novex WedgeWell SDS-PAGE gels (Invitrogen). Following electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified antigens were then biotinylated with the EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) using a 50:1 biotin to protein molar ratio for calculations.
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bioRxiv - Molecular Biology 2024Quote: ... ExpiFectamine CHO Enhancer and ExpiCHO Feed reagents (Thermo Fisher Scientific) were added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% FBS (Gibco), 5 U ml−1 Penicillin-streptomycin (Lonza ...
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bioRxiv - Immunology 2024Quote: ... Memory CD8+ T cells were activated with αCD3/αCD28 beads (1:1 cell:bead ratio, ThermoFisher). For cytokine measurements ...
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bioRxiv - Immunology 2024Quote: ... B16-OVA cells (44) and MEC.B7.SigOVA cells (38) were cultured at 37°C and 5% CO2 in IMDM (GIBCO-BRL) containing L-glutamine and 25mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... naive CD4+ T cells were activated with αCD3/αCD28 beads (1:1 cell:bead ratio, ThermoFisher) in the presence of TGFB (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using Superscript III Reverse transcriptase (Invitrogen). Quantitative Real-Time PCR was performed in triplicate with SYBR green on a StepOne Plus (both Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from sorted OT-I T cells and extracted using Trizol reagent (Invitrogen). cDNA was synthesized using Superscript III Reverse transcriptase (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Quantitative Real-Time PCR was performed in triplicate with SYBR green on a StepOne Plus (both Applied Biosystems) using previously described primers for Ifng and Rpl32 mRNA (39) ...
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bioRxiv - Immunology 2024Quote: ... 0.1% β-mercaptoethanol (Invitrogen), 1% nonessential amino acids (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were cultured in DMEM (Invitrogen) or calcium-free DMEM (Gibco ...
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bioRxiv - Immunology 2024Quote: ... or 48 hours post stimulation in the presence of 2.5μM IMD0354 (n=3) were collected into TRIzol reagent (15596026, ThermoFisher). RNA isolations were performed according to the manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: ... or calcium-free DMEM (Gibco) containing 10% FBS (Gemini) ...
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bioRxiv - Immunology 2024Quote: ... Viability was assayed before permeabilization using LIVE/DEAD (Invitrogen) fixable stain according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... using (p)-S6 Ser235/236 (monoclonal cupk43k, Thermo Fisher Scientific). Viability was assayed before permeabilization using LIVE/DEAD (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were cultured on 3.5 cm glass-bottom poly-D-lysine coated dishes from Mattek (catalog #P35GC-1.5-14-C) in Dulbecco’s Modified Eagle Medium (DMEM) from Gibco (catalog #11965-092) and supplemented with 10% cosmic calf serum from Cytiva Hyclone (catalog #SH30087.03 ...
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bioRxiv - Molecular Biology 2024Quote: ... The specific proteins were visualized using ECL western blotting system (ThermoFisher, USA). For quantitation of western blot band intensity ...
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bioRxiv - Molecular Biology 2024Quote: ... Capillary sequencing was performed using forward and reverse primers with the BIG dye terminator chemistry v3.1 (Applied Biosystems, UK), according to the protocol adopted from CDC in Atlanta ...
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bioRxiv - Molecular Biology 2024Quote: ... was performed on a Bio-Rad CFX384 Real-Time PCR Detection System using PowerUPTM SYBRTM Green Master Mix from ThermoFisher Scientific (catalog #A25742) ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of the extracted DNA was determined using the Qubit Fluorometric Quantitation system (Thermo Fisher Scientific, Waltham, USA).
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bioRxiv - Molecular Biology 2024Quote: Cells were stained using 3 mM stock solution of Nile Red (Thermo Fisher, Invitrogen) (used for staining membranes ...
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bioRxiv - Molecular Biology 2024Quote: Cells were stained using 3 mM stock solution of Nile Red (Thermo Fisher, Invitrogen) (used for staining membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% foetal bovine serum (FBS) (Gibco) and 5 U ml−1 Penicillin-streptomycin (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... They were then transfected with Halo-tagged gene constructs using Lipofectamine LTX (Thermo Fisher Scientific, MA, USA). After 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... and cells were lysed and collected in 500 μl TRIzol Reagent from ThermoFisher Scientific (catalog #15596018 ...
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bioRxiv - Molecular Biology 2024Quote: ... after which then genomic DNA was isolated for detection of gene editing efficiency using the GeneArt genomic cleavage assay kit (Invitrogen, catalog #A24372). Hep3Bcas9 cells had high cleavage efficiency and thus was chosen for generating knockout cell lines using CRISPR ...
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bioRxiv - Molecular Biology 2024Quote: ... and a #1 size coverslip (Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... AcTEV™ Protease (12575015) and PreScission Protease (27–0843-01) were obtained from Thermo Fisher Scientific and GE Health Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: The pellets were thawed and resuspended with 50 mL Tris-Buffered Saline (TBS) with an added protease inhibitor tablet (Thermo Scientific Pierce Protease Inhibitor Tablets EDTA-Free ...
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bioRxiv - Molecular Biology 2024Quote: ... or ProLong Glass Antifade mounting medium (Invitrogen, USA). For α-tubulin staining ...
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bioRxiv - Molecular Biology 2024Quote: ... coli strain) on the automated KingFisher Flex platform using the MagMax™ Viral/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific, UK). Post extraction DNase treatment was performed using the TURBO DNA-free™ Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The absorbance of the working SYPRO Orange solution was 0.295 at 468 nm (1 mm path length in a NanoDrop One, ThermoFisher Scientific). For each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... sodium pyruvate (Gibco) and pen/strep/glutamine (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FBS (Gibco), sodium pyruvate (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... in 100mm tissue-culture dishes (ThermoFisher). PDX models TM00244 ...
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bioRxiv - Cancer Biology 2024Quote: ... and pen/strep/glutamine (Gibco) in 100mm tissue-culture dishes (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were grown in DMEM with glucose (Gibco), supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... All the cell lines were maintained in standard Dulbecco’s modified Eagle’s medium (DMEM, high glucose, Life Technologies, #12077579) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% anti-anti (Antibiotic-Antimycotic, Gibco, #15240062), LIF (1000 U/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% heat inactivated fetal bovine serum (FBS) (Gibco, #10270106) and 1% anti-anti (Antibiotic-Antimycotic ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% anti-anti (Antibiotic-Antimycotic, Gibco, #15240062).
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bioRxiv - Cancer Biology 2024Quote: ... mESCs were grown on gelatin 1% with ES medium (EM): Dulbecco’s modified Eagle’s medium (DMEM, high glucose, Life Technologies, #12077579) supplemented with 15% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 15% foetal bovine serum (FBS) (Gibco, #10270106), 1% anti-anti (Antibiotic-Antimycotic ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol and resuspended in Formamide (ThermoFisher Scientific). 24 ul (5ug ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from these cultures and from parallel cultures of NIH3T3 using Trizol and GeneJet RNA micro kit (ThermoFisher Scientific). RNA sequencing libraries were created using the NEBNext mRNA stranded protocol (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was performed using Superscript IV (Invitrogen) with random hexamers ...
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bioRxiv - Molecular Biology 2024Quote: ... 100U of S1 Nuclease (ThermoFisher Scientific) in 270 ul of ice-cold S1 reaction buffer (30mM Na actetate ...