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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and then incubated with 50 μg Dynabeads Protein G/A (ThermoFisher) blocked with bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... The AciveXTM desthiobiotin-ATP probe (Thermo Fisher) was reconstituted in ultrapure water to 20 μM and 10 μL added to each sample followed by incubation for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... or an equivalent amount of the corresponding IgG Isotype control (ThermoFisher) and 30 μl of Dynabeads® Protein A/G (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... were loaded onto an EASY-nLC1200 system (Thermo Fisher Scientific) coupled with an EASY-Spray column (C18 ...
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bioRxiv - Molecular Biology 2024Quote: ... The fractionated peptides were then subjected to analysis on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Data acquisition was set to data-dependent mode ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA yields were determined using the Qubit RNA BR assay kit (ThermoFisher Scientific). cDNA was synthetized from 2ug of total RNA using MMLV-Reverse transcriptase and d(N)6 random primers in a 20ul reaction following the manufacturer’s protocol (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Appropriate volumes containing 20 µg of protein combined with NuPage LDS Sample Buffer and NuPage Reducing Agent (Invitrogen) were run on 4–12% NuPage Bis-Tris gels in MOPS buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthetized from 2ug of total RNA using MMLV-Reverse transcriptase and d(N)6 random primers in a 20ul reaction following the manufacturer’s protocol (ThermoFisher Scientific). RT-qPCR analyses were performed in triplicate using 2.5 μl of cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was recovered with 1 ml Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol and resuspended in Formamide (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... or PowerUp™ SYBR™ Green Master Mix (ThermoFisher) in a final volume of 20 ul ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... followed by DAPI staining and three PBT washes before mounting in ProLong Gold mounting media (Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... 20 nM of siRNA (sequences in Supp. Table 2) were mixed with Lipofectamine™ RNAiMax (ThermoFisher Scientific) following the manufacturer’s instructions and cells were harvested 48 h after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclear lysate was centrifuged for 15 min to remove cell debris and soluble proteins were quantified by BCA (ThermoFisher). The lysate was pre-cleared with 30 μg of Dynabeads Protein G/A (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol and resuspended in Formamide (ThermoFisher Scientific). One microgram of RNA was loaded onto a 1% formaldehyde/MOPS Buffer gel (Stefanovsky and Moss ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... All genomic DNA was amplified using Phusion polymerase (Invitrogen), and the repair matrix was assembled in pBluescript-II SK(+) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μg of purified RNAs were treated with Dnase I (ThermoFisher) and retrotranscribed using Maxima reverse transcriptase (ThermoFisher) ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... All genomic DNA was amplified using Phusion polymerase (Invitrogen), and the repair matrix was assembled in pBluescript-II SK(+) ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... 0–2 h embryos were homogenized in Trizol (Invitrogen) and RNA was extracted as directed by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... Lipofectamine 3000 was used according to the manufacturer’s protocol (Invitrogen). The following plasmids were transfected into U2OS ...
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bioRxiv - Molecular Biology 2024Quote: ... Grids were double blotted and subsequently plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) set to 100% humidity and 4 ℃ with a blot time of 5 seconds ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies) using the QuantiFast SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 20-40 μg of protein lysates were combined with 4X NuPAGE LDS sample buffer (Invitrogen) and 10X NuPAGE Sample Reducing Agent (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... The resolved samples were run on either NuPAGE 3–8% Tris-acetate or NuPAGE 12% Bis-Tris protein gels (Thermo Fisher Scientific) with the appropriate running buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in 2X NuPAGE LDS sample buffer (Invitrogen), and incubated at 95 °C in a heat block for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 200–500 μg of nuclear lysates were incubated with 2 μg of specific antibodies and 50 μg of Dynabeads Protein G (Invitrogen). Following an overnight incubation on a rotator at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Grids were blotted and subsequently plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) set to 100% humidity and 4 ℃ with a blot time of 5 seconds ...
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Assessment of salivary microRNA by RT-qPCR: Challenges in data interpretation for clinical diagnosisbioRxiv - Molecular Biology 2024Quote: ... Qubit™ microRNA Assay Kits (ThermoFisher Scientific) and Labchip small RNA assay (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... The default probe sets defined by Affymetrix were used to assess array quality using the Area Under the [Receiver Operating Characteristics] Curve (AUC ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted using TRIzol (Invitrogen) followed by miRNeasy mini column (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration and degree of labelling were determined using the Nanodrop 2000/2000c (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% Penicillin/Streptomycin (P/S, GIBCO) at 37 °C in a 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were detached from the plate and %GFP positive cells were measured on an Attune flow cytometer (ThermoFisher Scientific). Titer was quantified by the following calculation and reported as infectious units (IU ...
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bioRxiv - Cancer Biology 2024Quote: ... Absolute cell counts were quantified using CountBright™ Absolute Counting Beads (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and mounted with Permount (Thermo Fisher Scientific, Waltham, MA). For immunofluorescence staining ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 2.5U SUPERas-In (Thermo Fisher Scientific) in LoBind 96-well full skirted plates (Eppendorf ...
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bioRxiv - Cancer Biology 2024Quote: Protein concentration was determined using Pierce™ BCA™ Protein Assay Kit (Thermo Scientific 23225). 15-35 µg of protein samples were ran on precast 4-15% gradient gels (Bio Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... 15-35 µg of protein samples were ran on precast 4-15% gradient gels (Bio Rad) and transferred to nitrocellulose membranes (Invitrogen). 4-15% Mini-PROTEAN® TGX™ precast protein gel (Bio-Rad 4561084 ...
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bioRxiv - Molecular Biology 2024Quote: ... Wells were then stained with 2 μM Hoechst 33342 nuclear stain (Invitrogen H1399) and imaged on a Cytation C10 confocal plate reader (BioTek) ...
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bioRxiv - Molecular Biology 2024Quote: ... and DOT1L protein (IDs s39011, s39010; Ambion) was performed using Lipofectamine 3000 according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... EDTA-free (Thermo Scientific)) or RIPA lysis buffer (0.1% SDS ...
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bioRxiv - Cancer Biology 2024Quote: ... RKO and GP2D were cultured in DMEM (Thermo-Fisher Scientific#11965092), SW1463 were cultured in DMEM with 1mM Na-Pyruvate (Thermo-Fisher Scientific#11360070) ...
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bioRxiv - Cancer Biology 2024Quote: ... mixed with Iscove’s Modified Dulbecco’s medium (Gibco 12200-036) containing 20% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was incubated for 30 minutes at 4 ℃ before adding 10X CMC CHAPS (VitroEase™ Buffer Screening Kit, ThermoFisher Scientific) to a final concentration of 0.25X CMC ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated from 1-2 mg of total RNA using random hexamers and Maxima Reverse Transcriptase (Thermo Fisher Scientific). Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: The sample was loaded onto a Zeba Spin Desalting Column (7K MWCO, 0.5 mL, ThermoFisher Scientific), pre-equilibrated with 50 mM PB (pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The control cells were treated with the same concentration of the negative control silencer (Ambion, Cat. #AM4611). The protein extracts and the RNA samples were collected after 48 hours.
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bioRxiv - Molecular Biology 2024Quote: ... 1% glutamine (L-GLUT; GIBCO), and 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Molecular Biology 2024Quote: Alexa Fluor 488-maleimide (AF488) fluorophore and Alexa Fluor 647-maleimide (AF647) (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... NeutrAvidin (an analog of streptavidin) (ThermoFisher Scientific) was applied directly to the coverslip and incubated for 15 min before rinsing with Milli-Q water and drying with nitrogen gas.