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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Biochemistry 2020Quote: ... with either a C-terminal (Thermo Scientific) or N-terminal poly-histidine-tag (Genscript ...
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bioRxiv - Genomics 2021Quote: ... using Terminal Deoxynucleotidyl Transferase (TdT; Thermo Scientific) with the following reagent composition ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies for presynaptic terminals (SNAP-25; ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... using the Terminal Deoxynucleotidyl Transferase (Thermo Fisher)52 ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into the pFastBac1 vector with a 10xHis tag appended at the N terminus and was expressed in Sf9 cells (Gibco, catalog number 12659017). The cells were cultured at 27 °C for 60 h after being infected with baculoviruses using the Bac-to-Bac system (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... N’-tetrakis (2-pyridymethyl) ethylenediamine (TPEN) was from Molecular Probes (Waltham, MA, USA). Pisum sativum agglutinin (PSA ...
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bioRxiv - Cancer Biology 2019Quote: ... Gels were run using 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Invitrogen NP000202) and transferred onto nitrocellulose transfer membrane using an XCell II Blot Module or iBlot Dry Blotting System (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... SK-N-BE (2) and NGP cells were grown in RPMI1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... SMPCs were switched to N2 media (containing 1% N-2 supplement (Thermo Fisher), 1% Insulin Transferrin Selenium ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Cell Biology 2023Quote: ... 0.173% 2-methoxy(polyethyleneoxy)propyltrimethoxysilane (Gelest, SIM6492.7) and 0.62% n- butylamine (Acros Organics) with flowing nitrogen gas for 90 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... the plate containing the lysed cells is supplemented with RNA spike-ins (1 μL of a 1:1,600,000 dilution of ERCC spike-in mixture A (Life Technologies)) ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was diluted with 400 μl of NET-2 buffer and antibody-conjugated Dynabeads M-280 Sheep anti-mouse IgG (ThermoFisher Scientific) beads were added ...
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bioRxiv - Microbiology 2021Quote: ... 4 % FCS (Gibco), 200 units/mL penicillin and 0.2 mg/ mL streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS (InVitrogen) and either 1 µg/mL puromycin (DIvA cells ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Gibco), 1% non-essential amino acid (NEAA ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco) in RPMI ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% FCS (Invitrogen), 5% human serum (HS) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (Gibco), 1X Pen-Strep (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 % FCS (Gibco), 1 % PenStrep (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... 44% FCS (Gibco), and 6% DMSO (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... 5% FCS (Gibco), and 1x penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... 10% FCS (Gibco), penicillin/streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... N,N,N’,N’- tetramethyl ethylenediamine and glycylglycine were purchased from Acros Organics (New Jersey, USA). Protogel 30% acrylamide ...
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bioRxiv - Developmental Biology 2022Quote: ... WIK 24hpf samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 1 (4456740, Thermofisher Scientific) while all other samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 2 (4456740) ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of total RNA was mixed with 2 μl of 1:100 diluted ERCC RNA spike-in (Life Technologies), then ribosomal (r)RNAs were depleted using the RiboMinus Eukaryote v2 Kit (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... forward and reverse primers with overlapping SARS-CoV-2 spike sequence with the N501Y mutation followed by sequence complementary to the target plasmid (pEP-KanS) were synthesized (Invitrogen). A PCR fragment with overlapping ends carrying SARS-CoV-2 sequence flanking the Kanamycin resistance marker was amplified with the primers described using pEP-KanS as template ...
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bioRxiv - Cancer Biology 2019Quote: ... Input for each sample consisted of 500 ng of RNA and 5 μL of 1:500 diluted ERCC spike-in mix 2 (Ambion). Libraries were amplified for 11 cycles during the final amplification step ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was mixed with 2 µl of diluted ERCC RNA spike-in mix (1:100 dilution in nuclease-free H2O; Invitrogen). Ribosomal (r)RNAs were depleted using the Ribominus Eukaryote v2 kit (Ambion) ...
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bioRxiv - Genetics 2020Quote: ... or HA-Tag (Invitrogen) antibody for 1 h at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... TMT10plex tags (Thermo Scientific) were dissolved in anhydrous ACN and added to the peptide sample in a 1:10 peptide:TMT ratio ...
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bioRxiv - Biochemistry 2023Quote: ... anti-SNAP tag (Invitrogen), and DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
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bioRxiv - Bioengineering 2021Quote: ... N,N′-Diisopropylcarbodiimide (DIC, Fisher Scientific) and 10 equivalents of DIPEA in DMF for 4 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar: BD Difco, Fisher Scientific; defibrinated sheep’s blood: Thermo Scientific, Fisher Scientific), and M9 minimal media (BD Difco ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Immunology 2024Quote: ... We electroporated 3 μg of ODNs or gDNA into 2 × 106 HEK293 and 293A cells by Neon Transfection System (Thermo Fisher) based on the manufacturer’s introductions.
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 and OC43 were propagated in Vero E6 cells (ATCC) grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 0.2 μg empty pQCXIP or pQCXIP-WT IFITM3 or the indicated mutant fused with amino-terminal FLAG using Lipofectamine2000 (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... dUTP was added to DNA ends by pelleting stored cells and resuspending in 100 μL of elongation buffer (1X terminal deoxytransferase [TdT] buffer, 2.5 mM CoCl2, 0.3 mM 5-bromo-2’-deoxyuridine [BrdU] [Invitrogen, Waltham, MA]), then adding 5 U of TdT (Thermo-Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... and transfected with a total of 2 μg C-terminal EGFP tagged M2 tail using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer protocol after washing with 200 μL PBS and refreshing with 100 μL DMEM (ScienCell ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads Sheep anti-rat igG was obtained from Invitrogen, life technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-sheep Alexa 555-conjugated (Life Technologies, A21436) 1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... donkey anti-sheep 594 (1:500, Thermo Fisher, A11016). Slides were then washed 3 times for 10 min each in PBT and subsequently stained with 0.1 μg/ml DAPI in PBS for 10 min ...