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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and/or hygromycin (Invitrogen; U-2 OS: 250μg/mL, Huh7.5: N/A). BHK-21J cells were propagated in “complete” MEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS buffer containing 1× N-2 supplement (Gibco, Thermo Fisher Scientific) and 0.1% SDS and then analyzed by second-generation RT-QuIC assay ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS buffer containing 1× N-2 supplement (Gibco, Thermo Fisher Scientific) and 0.1% SDS and then analyzed by second-generation RT-QuIC assay ...
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bioRxiv - Molecular Biology 2023Quote: ... and plated in N-2 media consisting of Neurobasal-A medium (Invitrogen) supplemented with N-2 Supplement (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.5 μl of TEMED (N,N,N’,N’-tetramethylethylenediamine; 15524-010, Invitrogen). A 8 μl drop was quickly deposited onto each 19 mm coverslip ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... After staining of the transferred proteins with amido black the cleavage product was cut out for sequencing via N-terminal Edman degradation performed on a Procise Protein Sequencer 494C (Applied Biosystems, Foster City, United States).
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bioRxiv - Cell Biology 2023Quote: ... Expression constructs were made by LR reaction using the following destination vectors: pDEST15 (for N-terminal GST-fusion in E. coli, cat#11802014, Thermo Fisher Scientific, Waltham, MA, USA) and pcDNA-DEST53 (for N-terminal GFP fusion in mammalian cells ...
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bioRxiv - Cell Biology 2022Quote: ... The protein band corresponding to Fragment 2 was cut out and subjected to N-terminal sequencing analysis (Matsudaira, 1987) on an automated protein sequencer (#Procise 491 cLc; Applied Biosystems, Foster City, CA, USA) using the standard pulsed-liquid program for PVDF-blotted proteins.
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bioRxiv - Cell Biology 2023Quote: ... In the case of sheep polyclonal anti-H2A.Z (acetyl K4+K7+K11, ab18262) Alexa fluor 633 (A633) conjugated goat anti-sheep IgG (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Purifications were carried out from 150 μl of the whole-cell extract (2 mg protein) per experiment using 37 µl of Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen) and 2 µl of the anti-LytA antibody (Biomedal) ...
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bioRxiv - Bioengineering 2024Quote: ... at a 1:200 dilution for 2 h at room temperature (RT) followed by donkey anti-sheep Alexa Fluor 647 secondary antibody (Invitrogen) at a 1:400 dilution and donkey anti-mouse Alexa Fluor 555 antibody (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... GST-tag and 3xFLAG-tag fusions were achieved using pDEST27 (Invitrogen) and pCI-neo-3xFLAG vector ...
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bioRxiv - Molecular Biology 2020Quote: The remaining RNA was combined into four final bsRNA-seq fractions (Figure 1) and 10μg RNA was spiked with ERCC Spike-in Mix 2 (Ambion) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... A 2 μl aliquot of a 1:10 dilution of the ERCC RNA Spike-in Mix (Thermo Fisher Scientific) were added to each sample ...
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bioRxiv - Genomics 2021Quote: ... 2 µg of RNA from each sample was incorporated with ERCC ExFold RNA Spike-In mixes (Thermofisher Scientific, 4456739) following the manufacturer’s guideline ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl per well of 2 μg/ml Spike trimer or RBD in 1x PBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 2 µg/ml spike protein diluted in PBS was incubated over-night on 96-well high affinity plates (Nunc). To prevent unspecific binding ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740).
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740). Libraries were prepared using the Kapa RNA HyperPrep Kit with RiboErase (Roche #08098131702) ...
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bioRxiv - Developmental Biology 2020Quote: ... and sheep anti-eGFP (1:500, Invitrogen, 10396164). The following secondary antibodies were used ...
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bioRxiv - Microbiology 2022Quote: ... and donkey anti-sheep AF568 (A-21099, Invitrogen) diluted in 4% BSA in PBS for 1 hour at RT ...
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bioRxiv - Genomics 2020Quote: ... antibodies and Sheep anti-Mouse IgG Dynabeads (Invitrogen) were used ...
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bioRxiv - Microbiology 2020Quote: ... defibrinated sheep red blood cells (RBCs) (Fisher Scientific) were washed twice and diluted to 1% (vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... coupled Dynabeads® Sheep Anti-Rat IgG (Invitrogen, 50 µl/ brain ...
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bioRxiv - Molecular Biology 2022Quote: ... Dynabead M-280 Sheep Anti-Mouse IgG (Invitrogen) magnetic beads were washed three times using TBS+0.05% Tween-20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Defibrinated sheep erythrocytes (Oxoid-Thermo Fisher, the Netherlands) were washed and diluted in DMEM ...
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bioRxiv - Cell Biology 2019Quote: ... and sheep anti-rat Dynabeads (ThermoFisher Scientific, 11035) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Dynabeads (sheep anti-mouse IgG,11201D Life Technologies) were pre-incubated with anti-FLAG M2 antibody (F1804 ...
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bioRxiv - Developmental Biology 2019Quote: ... -rabbit or –sheep secondary IgG antibodies (Thermo Fisher). DNA was counterstained with 5 µg/ml Hoechst 33342 (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... and Rabbit Anti-Sheep HRP (Thermo Scientific, 31480). Blots were imaged after 5 minutes in the presence of Super-Signal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... polyclonal sheep anti-copeptin (a gift from ThermoFisher) and ...
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-sheep IgG-Alexa488 (#A-11015, Invitrogen). Free-floating sections were mounted on microscope slides and immunohistochemistry staining was visualized with the Zeiss LSM 710 confocal microscope (Zeiss).
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bioRxiv - Immunology 2023Quote: ... and Dynabeads® sheep anti-rat IgG (Invitrogen), and resuspended in complete RPMI ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies: sheep 488 and mouse 647 (Invitrogen)
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bioRxiv - Cell Biology 2023Quote: ... donkey anti- Sheep 488 (A11015; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... which was generating by replacing a V5 tag with a 3HA tag or a 3flag tag from pcDNA3.1-nV5-DEST vector (Invitrogen), and E ...
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bioRxiv - Developmental Biology 2021Quote: ... ERCC spike-in RNAs (4456653; ThermoFisher Scientific) were added at a 1:40 dilution of stock at 1µl per embryo (5 embryos and 5 µl diluted ERCC RNA per sample ...
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bioRxiv - Genomics 2020Quote: ... ERCC RNA Spike-In Mix 1 (Invitrogen), and the mCherry and EGFP open reading frames from the FUCCI plasmid (we included the latter to ensure that the transgene was being transcribed) ...
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bioRxiv - Neuroscience 2020Quote: ... and ERCC Spike-ins (ThermoFisher Scientific 4456740) diluted at 1:2.4×107 ...
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bioRxiv - Bioengineering 2020Quote: ... 1:600,000 diluted ERCC spike-in (ThermoFisher), and 32 µL of 10% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... and ERCC spike in (Thermo Fisher #4456740) was added ...
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bioRxiv - Microbiology 2021Quote: ... ERCC RNA Spike-In Control Mixes (Ambion) was added proportionally to the total RNA of each sample (0.1 µL per sample ...
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bioRxiv - Cell Biology 2022Quote: ... ERCC spike ins (ThermoFisher cat. num. 4456739) were added to each sample in a dilution ranging from 1:1000 to 1:10000 depending on the initial amount of RNA extracted.
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bioRxiv - Biochemistry 2022Quote: ... ERCC RNA Spike-In Mix (both Invitrogen) was added according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1:4000000 ERCC spike-in (Ambion, 4456740), 2.5 mM dNTPs (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... and ERCC spike-in synthetic RNAs (Ambion). 96-well plates were snap frozen and stored at –80 °C until use ...
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bioRxiv - Immunology 2020Quote: ... and ERCC RNA spike in controls (Ambion)) in a 96-well V-bottom PCR plate (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: ... ERCC ExFold RNA Spike-in mix (Invitrogen) was added to purified RNA ...
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bioRxiv - Cell Biology 2019Quote: ... ERCC Mix 1 spike-in controls (ThermoFisher) were added at 1e-5 final dilution ...