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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The HEK293 cell line was obtained from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID: CVCL_6911) was purchased from Thermo Fisher Scientific (#R70007) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μl of the resulting 1:10 dilution were mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher) and subjected to library preparation for next-generation sequencing (vertis Biotechnologie) ...
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bioRxiv - Physiology 2019Quote: ... Strand-specific RNA-seq libraries containing unique index sequences in the adapter were generated simultaneously following the dUTP method using 700 ng total RNA and ERCC spike mix 2 (Ambion, 4456740). Libraries were pooled and sequenced on Illumina HiSeq 2500 in 75bp paired-end mode ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (Hexapro antigen from Erica Ollmann Saphire, La Jallo for Immunology) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with sera samples to allow antibody binding to occur ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten- micro-liter of the aliquot was mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher). The resulting RNA samples were fragmented ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of RNA of each sample was mixed with 2 µl of ERCC ExFold spike-in RNA mixes based on the RNA ratio recommended by the protocol (Invitrogen 4456739), rRNA were depleted by hybridization of complementary DNA oligonucleotides and treatment with RNase and DNase ...
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bioRxiv - Bioengineering 2022Quote: ... Final water-PAAm mixtures were crosslinked with 0.5% APS and 0.15% N,N,N′,N′-tetramethylethylenediamine (ThermoFisher, 17919). 50 μL of the mixtures were added to each glass and allowed to crosslink for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... RBDs or NTD was performed by coating Nunc MaxiSorp flat-bottom high-binding 96-half-well plates (ThermoFisher Scientific, MS, USA) with 50 μl per well of a 0.1 μg ml−1 protein solution in PBS overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... NPCs were plated on Geltrex in a 6 well plate in StemPro NSC SFM supplemented with 20ng/ml of EGF and bFGF for 2 days before switching to astrocyte differentiation medium consisting of DMEM with 1% N-2 supplement (Gibco), 1% GlutaMAX-I supplement (Gibco ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We subsequently placed two frozen individuals from each family and treatment group (n = 9 families * 2 treatments (+/− supplemental thiamine) * 2 individuals = 36) into 10 volumes of RNAlater-ICE (Invitrogen) pre-chilled to −80 °C and allowed the samples to reach −20 °C overnight ...
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-APP C-Terminal (clone CT695; Invitrogen), mouse anti-APP N-terminal (clone 22C11 ...
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bioRxiv - Immunology 2022Quote: ... with terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in Gaspar et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Terminal deoxy transferase (TDT) was obtained from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... and terminal deoxynucleotidyl transferase (TdTase ThermoFisher Scientific #10533065). The reaction was kept at room temperature for 3-4 days and poly-dT was purified using phenol chloroform extraction and suspending the air-dried pellet in water ...
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bioRxiv - Immunology 2021Quote: ... with terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in (Gaspar et al. ...
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bioRxiv - Immunology 2021Quote: ... with terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in (34).
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bioRxiv - Molecular Biology 2023Quote: ... they reacted with terminal deoxynucleotidyl transferase enzyme (Invitrogen) and biotinylated 16-dUTP (Roche Diagnostics ...
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bioRxiv - Cell Biology 2024Quote: ... and terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in Gaspar et al ...
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bioRxiv - Cancer Biology 2024Quote: ... FOXA1 C-terminal (Thermo Fisher Scientific: PA5-27157); AR (Millipore ...
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bioRxiv - Immunology 2021Quote: ... The preparation of eVLPs expressing either Wuhan reference Spike or Beta variant Spike were formulated in Aluminum phosphate (Alum, Adjuphos®, Invitrogen) to obtain vaccine candidate VBI-2902a and VBI-2905a ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using D614G Spike and Delta Spike gene-specific primers and probes and TaqMan Fast Advanced Master mix (Cat# 4444963, Applied Biosystems) on a QuantStudio 5 system (Applied Biosystem) ...
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bioRxiv - Microbiology 2021Quote: ... and 0.144 µg pCG1-SARS-2-S or its derivative or 0.072 µg pCMV-VSVG as a control using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Immunology 2021Quote: RBDwt of SARS-CoV-2 and mutant RBDs (RBDK417N, RBDE484K, RBDN501Y, RBDK417N/E484K/N501Y, and RBDL452R/E484Q) were expressed using Expi293F cells (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: Phase diagrams of non-phosphorylated and phosphorylated NSARS-CoV-2 at different concentrations were determined using a NanoDrop spectrophotometer (ThermoFisher Scientific, Invitrogen). Increasing concentrations of polyU (0 - 2 µM ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...
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bioRxiv - Pathology 2021Quote: ... 10 µl of rSARS-CoV-2 NL virus stock was diluted in 10 ml of OptiMEM media (ThermoFisher Scientific, #51985091) and then added to cells ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Hydroxybenzotriazole (HOBt) and O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Fisher Scientific. Trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2021Quote: Whole ear pinnae homogenates were diluted in 10-fold steps in LB medium and 10 µl of undiluted and diluted samples were pipetted onto sheep blood agar plates (Columbia Agar with sheep blood Plates; Oxoid, Cat. # PB0123A, Thermo Fisher Scientific, Waltham, Massachusetts). After incubation for 18 h at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... Chromatin complexes were precipitated with 100 µl of Dynabeads M-280 (Sheep anti-mouse, # 11202D; sheep anti-rabbit, #11204; Thermo Fisher Scientific, Waltham, MA). After washes and elution ...
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bioRxiv - Plant Biology 2021Quote: ... or GLK2 was recombined into the destination vector pGWB605 for C-terminal fusion with sGFP or into pGWB617 for C-terminal fusion with 4×Myc through the Gateway LR reaction (Thermo Scientific). For the 35S:GLK1-4×Myc (or sGFP ...
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bioRxiv - Plant Biology 2019Quote: ... or the stop codon-less full CDS of PNPAR was recombined into the destination vector pGWB651 for C-terminal fusion with GFP or pGWB617 for C-terminal fusion with 4xMYC through Gateway LR reaction (Thermo Scientific) to create p35S:tPNP-A-GFP and p35S:PNPAR-4xMYC ...
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bioRxiv - Cell Biology 2020Quote: ... plus 10 % FCS (Gibco, Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1% FCS (Gibco). Followed by 3 days of culture ...
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bioRxiv - Bioengineering 2019Quote: ... FC from Life Technologies; and 2 ...
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bioRxiv - Neuroscience 2020Quote: ... containing 10% FCS (Invitrogen), and gentamycin (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... dialyzed FCS (Thermofisher, # 26400044), 28 μg/mL L-arginine-HCl (Arg0 or Arg10 ...
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bioRxiv - Biochemistry 2021Quote: ... including 10% FCS (Gibco), 2 mM glutamine (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... with 10 % FCS (Gibco), 2 mM glutamine (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... containing 10 % FCS (Gibco). Macrophages were seeded into 24-well plates at 105 cells per well.
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bioRxiv - Microbiology 2022Quote: ... containing 10% FCS (Gibco), 40 mM Hepes (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5% FCS (Gibco) in PBS with 0.01% Tween-20 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco, 26400044), 2.5% 1M HEPES buffer (Sigma ...