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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... or donkey anti-sheep-AlexaFluor568 ABs (Thermo Scientific, cat.# A21099) were used except for the V5 condition ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Cancer Biology 2021Quote: ... NC1 domain was digested with trypsin (MS Grade, Thermo Fisher Scientific) overnight at 37 °C and then analyzed by LC-MS analysis on a Shimadzu UFLC 20ADXR HPLC system in-line with an AB Sciex 5600 Triple TOF mass spectrometer (AB SCIEX ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2-Spike construct was expressed in Spodoptera frugiperda Sf9 cells following instructions from bac-to-bac baculovirus expression system (Thermo Fisher, SG). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed twice with ELISA wash buffer to remove any excess unbound spike protein and blocked with 2% BSA (ThermoFisher Scientific; 37525) in ELISA wash buffer overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... were coated with 50 μL per well of 2 μg/mL Spike trimer or RBD in 1 x PBS (Fisher Scientific, 11510546) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of this dilution was mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher Scientific) and subjected to library preparation for next-generation sequencing (Vertis Biotechnologie ...
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bioRxiv - Bioengineering 2023Quote: ... N,N’-Dicyclohexylcarbodiimide (DCC, 0.02 mol; Fisher Scientific) was dissolved in DCM with Norbornene-2-carboxylic acid (0.04 mol ...
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bioRxiv - Cancer Biology 2019Quote: ... HF2597 cells were grown in DMEM/F12 containing 1% N-2 Supplement (Gibco 17502-048), 500 mg/l BSA ...
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bioRxiv - Neuroscience 2021Quote: ... medium was changed to DMEM/F-12 supplemented with 1× N-2 supplement (Life Technologies), 2 µg/mL heparin ...
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bioRxiv - Neuroscience 2021Quote: ... iNeurons were dissociated and grown in Neurobasal medium containing N-2 supplement (Gibco, cat# 17502048), B-27 supplement ...
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bioRxiv - Genomics 2019Quote: ... and grown in proliferation medium: Advanced DMEM/F-12 plus N-2 Supplement (Thermo Fisher), 2 mM L-glutamine (Sigma ...
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bioRxiv - Evolutionary Biology 2021Quote: ERCC RNA Spike-In Mix (Thermo Fisher Scientific 4456740) was used in library preparation ...
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bioRxiv - Developmental Biology 2020Quote: ... containing the ERCC RNA spike-in Mix (Ambion, #4456740) diluted (1:100.000 ...
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bioRxiv - Molecular Biology 2021Quote: ... ERCC RNA Spike-in control mixes (Ambion, catalogue #4456740) were added to 1µg of total RNA according to manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... spiked-in with ERCC RNA Spike-In Mix (Ambion) and analyzed on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... ExFold RNA Spike-In Mixes (ERCC, Thermo Fisher Scientific) were added to each sample for normalizing gene expression ...
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bioRxiv - Neuroscience 2022Quote: ... we included ERCC spike-in controls (Ambion, cat#4456740) at a 1:106 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... ERCC RNA spike-in mix (Thermofisher Scientific, Waltham, Massachussetts) was also added to the solution for further amplification quality filtering (dilution 1/40.10e7) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1:500000 ERCC RNA spike-in mix (Ambion, 4456740)) ...
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bioRxiv - Microbiology 2020Quote: ... After adding ERCC RNA Spike-In Mix (Life Technologies), the sequencing library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB) ...
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bioRxiv - Immunology 2020Quote: ... the spike genes were synthesized by GeneArt (Life Technologies). The SARS-CoV-1 (1255 amino acids ...
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bioRxiv - Microbiology 2022Quote: ... ERCC RNA Spike-In Control mixes (Life Technologies, USA) were added to 5 μg of total RNA ...
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bioRxiv - Plant Biology 2022Quote: ... One nanogram of ArrayControl™ RNA Spikes (Invitrogen, USA) was added during tissue homogenization ...
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bioRxiv - Biochemistry 2022Quote: ... ERCC RNA spike-in control (4456740, Thermo Fisher Scientific) was added to each sample (0.1 µL per sample) ...
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bioRxiv - Molecular Biology 2022Quote: A synthetic spike-mix (ERCC, Thermofisher, Catalog Number 4456740) was used as a source of long RNAs for the sequencing experiments and as a spike in control for the Nanopore experiments involving biological samples ...
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bioRxiv - Cancer Biology 2023Quote: ... and ERCC spike-in Mix 1 (Thermo Fisher, #4456740) was added to the samples.
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bioRxiv - Cancer Biology 2023Quote: ... and 1:8,000,000 diluted ERCC spike-in mix (Ambion). 25 μL of lysis buffer was dispensed into each well of a 384-well stock plate using a Formulatrix Mantis with a high-volume chip ...
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bioRxiv - Systems Biology 2023Quote: ... ERCC spike-in (1:100,000 dilution, Thermo Fisher Scientific) was added to the reverse crosslinking mix ...
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bioRxiv - Genomics 2023Quote: ERCC RNA Spike-In Controls (Thermofisher, catalogue number: 4456740) were employed to establish a consistent baseline measurement of RNA ...
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bioRxiv - Biochemistry 2023Quote: ... While ERCC RNA Spike-In Mix (Invitrogen™, 4456740) was added to isolated RNA samples prior to sequencing ...
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bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... 6-well plate format) infected (MOI of 0.01) with WT or ΔORF rSARS-CoV-2 was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: 1 μg of SARS-Cov-2 S protein was diluted in loading buffer and loaded on 10–20% Novex Tricine pre-cast gel (Invitrogen, USA). The run was performed at 120 V for 1 h ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S 2P ectodomain and RBD constructs were produced by transient transfection of 500 mL of Expi293 cells (Thermo Fisher) and purified from clarified transfected cell supernatants four days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences) ...
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bioRxiv - Cell Biology 2020Quote: ... For transient overexpression (Fig 2 E-H, S2G) seipin tagged with C-terminal EGFP [34] was inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific) through HindIII/NotI sites ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney cells (HEK293) were maintained in IMDM (Gibco) supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293) T-Rex Flp-In (ThermoFisher Scientific) cells were grown in DMEM supplemented with 10% FBS in an atmosphere of 5% CO2 ...
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bioRxiv - Developmental Biology 2019Quote: HEK293 (Flp-In™-293 Cell Line, Thermo Fisher Scientific) were grown in DMEM supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2019Quote: HEK293-SUMO3m cells were grown in DMEM (Thermo Fisher Scientific) containing light (0Lys ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Invitrogen) with 5% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were maintained in DMEM (Thermo Fisher Scientific, 10569010) supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells or FreeStyle 293F cells (Invitrogen, Waltham, MA, USA) were cultured in DMEM medium with 10% FBS and 1% Pen-Strep at 37°C with 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and pHelper62 into HEK293 cells using Lipofectamine LTX (Invitrogen 15338030) following standard protocols62 ...
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Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoproteinbioRxiv - Biophysics 2020Quote: ... The construct was transiently transfected into HEK293 cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were maintained in DMEM (High Glucose DMEM; Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 Flp-In T-Rex cells (Thermo Fisher Scientific, R78007) were cultured in DMEM + GlutaMAX-I (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293 was grown in DMEM (Thermo Fisher, USA, Cat# 11995065) supplemented with 10% FBS (HyClone ...
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bioRxiv - Cancer Biology 2020Quote: ... VCaP and HEK293 were grown in DMEM with Glutamax (Gibco); LNCaP ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In™ T-REx™ HEK293 parental cells (Invitrogen) were seeded at 1×106 cells per 10 cm culturing dish in 15 ml media (DMEM High Glucose ...