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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for N acetylglucosamine 1 phosphodiester alpha N acetylglucosaminidase NAGPA Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Bone explants (n=8) were cultured in medium consisting of α-MEM (41061, GibcoTM, Thermo Fisher Scientific), 10% v/v FBS (HycloneTM ...
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bioRxiv - Cell Biology 2022Quote: ... Qiagen) in Optimem reduced serum media and lipofectamine (1μl/100 μl Optimem, RNAiMAX, Invitrogen, Cat. N. 56531) was added to the HUVEC and incubated overnight ...
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bioRxiv - Zoology 2023Quote: ... and 2-(N-morpholino)ethanesulfonic acid (MES) running buffer solution (Bolt™, Thermo Fisher Scientific, Waltham, Massachusetts). A standard ladder (Mark12™ ...
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bioRxiv - Biochemistry 2022Quote: ... Target protein was coated overnight (O/N) at 4 °C on MaxiSorp™ 96-well plates (Nunc). ELISA-blocking buffer (PBS containing 0.5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: A cDNA encoding p38α was subcloned into pcDNA6 with an N-terminal myc tag (Invitrogen, Carlsbad, CA), and a kinase-dead (D168A ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples (n = 3 technical replicates, each condition) were sorted with an Attune NxT flow cytometer (Thermo Fisher) and the DNA content was analyzed for the percent of cells in G1 ...
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bioRxiv - Neuroscience 2023Quote: ... was added to 10 ml of ice-cold N-PER Neuronal Protein Extraction Reagent (# 87792, Thermo Fisher) by vigorous vortexing until the tablet was completely dissolved ...
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bioRxiv - Neuroscience 2023Quote: The resulting peptide mixture was resolved by liquid chromatography (LC) using an EASY n-LC (Thermo Scientific) UHPLC system with buffer A (2% v/v acetonitrile/0.1% v/v formic acid ...
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bioRxiv - Cancer Biology 2024Quote: METTL3 siRNAs were used to induce transient knockdown in SK-N-BE(2) cells with RNAiMAX (ThermoFisher) reagent ...
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bioRxiv - Bioengineering 2024Quote: ... Unreacted maleimides were quenched by incubating the microgels in 100 mM N-acetyl-L-cysteine (Acros Organics) overnight at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... we purified the recombinant Rpf protein with the N-terminus polyhistidine-tag via Ni-NTA Purification (Invitrogen) using a 10 mL gravity-fed column with a 2 mL resin bed ...
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bioRxiv - Immunology 2024Quote: ... Control cohorts received 2 doses of: 104 PFU YF-S0*-vaccine [16] (n = 6) or MEM (Gibco) supplemented with 2% FCS (HyCloneTM ...
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bioRxiv - Immunology 2024Quote: Recombinant H3 (SinoBiological, 40859-V08H1) was immobilized on N-hydroxysuccinimide (NHS)–activated agarose resin (Thermo Fisher, 26197) by overnight rotation at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... protonation followed with 12 N HCl and methylation with 12% BF3-methanol (Thermo Fisher Scientific cat# AC402765000), as described previously in Plourde et al.111,112
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bioRxiv - Physiology 2024Quote: ... Additional males (n = 18) were administered either 50 nM of unconjugated quantum dots (QTracker 655 Fisher Scientific/Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... TIRMyD88 (residues 148-296) with an N-terminus myc-epitope was also cloned into pcDNA3.1+ vector (Invitrogen) for the luciferase activity assay ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmids encoding wild-type or N-glycosylation mutants of GPR52 were transfected into HEK293F cells (Gibco) cultured at 37 ℃ in 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... pET-11a expression vector carrying SARS-Cov-2 N gene was transformed in BL21 (DE3) cells (Invitrogen). Cells were grown in LB media at 37°C until reaching an optical density 0.5-0.6 at 600 nm ...
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bioRxiv - Bioengineering 2024Quote: ... The pH of the reaction was adjusted to 5 using 10 N HCl (Fisher Scientific, Hampton, NH), with temperature kept below 50 °C using ice packs to avoid gelatin denaturation ...
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bioRxiv - Cancer Biology 2024Quote: ... Sense target cDNA was prepared using the Affymetrix GeneChip WT Plus kit (Applied Biosystems P/N 902281). It was then fragmented and biotin-labeled using the GeneChip WT Terminal Labeling kit (Applied Biosystems P/N 900670 ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-mouse Ig-FITC (1:100,ThermoFisher,USA). Coverslips were mounted using Vectashield-DAPI-mounting-medium (Vector Laboratories,USA) ...
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bioRxiv - Physiology 2024Quote: ... FITC-anti-mouse F4/80 (Invitrogen #11480181, 1:100), PE-anti-mouse MERTK (BioLegend #151506 ...
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bioRxiv - Neuroscience 2024Quote: ... FITC conjugate (Thermo Fisher Scientific A90-234F, 1:200); Rabbit anti-Mfsd2a (Cell Signaling 80302 ...
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bioRxiv - Immunology 2024Quote: ... or CD49b – FITC (clone: DX5, 1:200) from Invitrogen.
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bioRxiv - Genomics 2024Quote: ... FITC (1:100, Thermo Fisher Scientific, 11-5981-82) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... A fluorescein isothiocyanate (FITC)–labeled goat anti-mouse antibody (Thermofisher, Waltham, MA) was added to the solution at the concentration of 0.5 g mL-1 ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescein (FITC)-conjugated mouse monoclonal anti-CD14 antibody (clone TuK4, Life technologies, Thermofisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... A FITC-conjugated anti-Influenza A NP monoclonal antibody (ThermoFisher MA1-7322) at 1:20 dilution was used to detect viral proteins using flow cytometry.
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bioRxiv - Cell Biology 2023Quote: Alexa 647 labelled Goat anti-Rabbit IgG secondary antibody and anti-alpha tubulin primary antibody was purchased from Thermo Fisher Scientific (Australia) ...
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bioRxiv - Immunology 2023Quote: ... FITC (Invitrogen), applied for 72 hours at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The sections were rinsed in TBST and further incubated with the secondary antibody of goat anti-mouse FITC (1:500) or goat anti-rabbit Alexa Fluor-647(1:500) (Life Technologies, Thermo Scientific, USA) or goat anti-Guinea pig Alexa Fluor 555(1:500)for 2 h at room temperature in a humid chamber ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated overnight at 4°C with the following primary antibodies diluted in 1% Normal Goat Serum: Rat anti-Ki67 conjugated FITC (1:50, Invitrogen, 11-5698-82), Mouse anti-active/pro-caspase3 (1:20 ...
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bioRxiv - Bioengineering 2022Quote: ... The serum-free medium used for encapsulation was alpha minimum essential medium (alpha-MEM, Gibco) supplemented with 10 % bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... sorted cells were cultured on irradiated OP9DLL1 monolayers in Alpha-MEM (Alpha-MEM, ThermoFisher, 12000063) supplemented with 20% FBS (HyClone) ...
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bioRxiv - Biophysics 2022Quote: ... Cells were rinsed 3 times by 1X PBS and labeled with 500 μL of 1:100 anti-alpha Tubulin primary antibody (Thermo Fisher Scientific, 322500), 1:200 anti-Tomm20 primary antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Neuroscience 2022Quote: ... consisting of DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml 1% penicillin/ streptomycin (pen/strep; all Thermo Fisher Scientific, Waltham, MA, USA) + 20 ng/ml BDNF + 20 ng/ml GDNF + 20 ng/ml NGFb (all Peprotech ...
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bioRxiv - Genetics 2022Quote: ... Over the course of the following 4 days, the colonies were gradually transitioned to neural induction medium (NIM) (consisting of DMEM/F12, 1% N-2 supplement (Fisher Scientific, Cat #: 17-502-048), MEM non-essential amino acids (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Silencer® Select N°1 negative control siRNA or predesigned Silencer® Select siRNAs for ZNF827 (s45694, s45695, s45696) (Thermo Fisher Scientific, Waltham, MA, USA) were used for the transfection process ...
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bioRxiv - Plant Biology 2024Quote: Three replicates of 50 after-ripened mature seeds were sterilized as above and subsequently imbibed on moist nitrocellulose filter papers (Whatman n°1; Thermo Fisher Scientific; Waltham, MA, USA) placed on 55 mm Petri dishes containing solid MS/2 with or without 1 μM 2-cis ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells expressing TMPRSS2 proteins or its domains in 6-well plates were washed with PBS and incubated with EZ-Link™ Sulfo-N-Hydroxy Succinimide– SS-Biotin (0.25 mg/ml, 1 ml/well) (Thermo Fisher Scientific; Cat No: A44390) label for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in a complete culture medium containing 1 mg/ml FITC-dextran (70,000 MW FITC-dextran; Thermo Fisher Scientific), and incubated at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were incubated with the FITC-conjugated goat anti-mouse IgG (H + L) secondary antibodies (1:300, Molecular Probes) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were incubated for 1 h in the dark with a secondary antibody conjugated to fluorescein isothiocyanate (FITC; Invitrogen). Next ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in 150 µL of an antibody cocktail containing 1:200 anti-Ter119 (FITC; Thermo Scientific, 11-5921-82), 1:50 anti-CD31 (FITC ...
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bioRxiv - Neuroscience 2021Quote: ... and/or alpha-bungarotoxin Alexa Fluor 594 (1:500, Molecular Probes, B-13423) in incubation buffer ...
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bioRxiv - Physiology 2022Quote: ... C-IV-II and C-V-alpha (ThermoFisher Scientific, 45-8199; 1:2500). Secondary antibodies used were horseradish peroxidase–conjugated anti-mouse (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2023Quote: HIF-1α was evaluated using a HIF-1 Alpha ELISA Kit (Invitrogen™). Procedure steps were followed according to the manufacturer’s instructions and results were monitored at 450 nm ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-phospho-CamKII alpha/bata/delta (Thr305) (1:400, Thermofisher, PA5-37832). Fluorescence-conjugated secondary antibodies (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5 in 1X PBS then treated with 400 μg/ml sulfo-N-hydroxysulfosuccinimide-biotin (Thermo Fisher Scientific) prepared in the washing buffer for 40 min on an orbital shaker at 4°C ...