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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for N acetylglucosamine 1 phosphodiester alpha N acetylglucosaminidase NAGPA Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Biophysics 2021Quote: ... The silanized surfaces were incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were prepared from 5x106 cells treated with N-acetylcysteine (MilliporeSigma A7250) for 4 hours followed by stimulation/oxidation with 1 mM hydrogen peroxide (Fisher Scientific Canada, Mississauga ON) for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... alpha-MEM (Thermo Fisher Scientific) was supplemented with 2.5 % FBS ...
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bioRxiv - Genomics 2022Quote: ... a 1:100 dilution of Anti-Mouse CD45 antibody conjugated to FITC (Thermo Fisher Scientific, Cat#: 11-0451-810) and a 1:400 dilution of Anti-Mouse Thy-1.2 antibody conjugated to APC (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and followed by the incubation with the secondary antibody (FITC-goat anti-rabbit IgG, 1:250 dilution, #L43001 Invitrogen) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Antibodies (Human Siglec-1, PE conjugated Anti-Human CD14, FITC conjugated Anti-Human CD16) were obtained from Thermo Fisher Inc ...
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bioRxiv - Cancer Biology 2021Quote: SK-N-BE(2) and NB16 cells were seeded into 8-champer slides (Thermo Fisher Scientific, 154534) with a density of 6×103 cells/well overnight and treated with indicated treatment for 72h ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were counterstained with DAPI and slides mounted with Fluoromount-G (Invitrogen, Cat. N: 00-4958-02).
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bioRxiv - Cell Biology 2020Quote: ... PAA gel surface activation reagents were ethyl(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) solution (Fisher Scientific). Collagenase from Clostridium histolyticum was purchased from Sigma.
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Biochemistry 2020Quote: ... Rubisco holoenzyme was labeled at the N terminus with the fluorophore Alexa Fluor 532 NHS ester (ThermoFisher) according to manufacturer’s instructions (∼2 dye molecules bound per Rubisco holoenzyme) ...
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bioRxiv - Neuroscience 2020Quote: ... a subset of slices (n = 9) were post-processed with streptavidin-conjugated Texas Red (Molecular Probes, Inc), imaged with a Zeiss LSM 510 confocal microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... MRC5-N cell line was grown in Minimum Essential Medium Eagle (MEM-aplha, Gibco Life Technologies 22561021), RPE-1 cell line was grown in Roswell Park Memorial Institute Media (RPMI ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from tissues in adult flies (n=15 per genotype) using Trizol Reagent (Ambion). cDNA using oligo(dT ...
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bioRxiv - Plant Biology 2021Quote: ... 5-DAG seedlings were incubated in the dark in 10 μg/mL PI (Invitrogen, cat. n. P4170) for 10 min and then rinsed twice with water and observed under a Zeiss LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
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bioRxiv - Plant Biology 2020Quote: ... Tissue C and N content was measured with an elemental analyzer (FlashEA 1112, Thermo Scientific, Walham, MA), and the percentage of C and N per sample was calculated with the instrument’s software (Eager Smart ...
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bioRxiv - Developmental Biology 2020Quote: ... Poly-D-Lysine and N-Cadherin coated coverslips and cultured in plating media (Neurobasal (Gibco, #21103-049) medium supplemented with 10% heat-inactivated FBS and 1X penicillin/streptomycin/glutamine).
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bioRxiv - Developmental Biology 2020Quote: ... was used to transfer the WNK1 sequence into the Vivid Colors pcDNA6.2/N-YFP vectors (Thermo Fisher), which created the mammalian expression vectors with YFP fused to the N-terminal end of WNK1 (Fig ...
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bioRxiv - Genomics 2021Quote: ... DNA quantification was performed using a Qubit™ 1X dsDNA HS Assay Kits (ThermoFisher, cat. n. Q33230), and DNA quality assessed with an Agilent 2100 Bioanalyzer.
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bioRxiv - Cancer Biology 2020Quote: ... MRC5-N cell line was grown in Minimum Essential Medium Eagle (MEM-aplha, Gibco Life Technologies 22561021), RPE-1 cell line was grown in Roswell Park Memorial Institute Media (RPMI ...
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bioRxiv - Genetics 2021Quote: ... 2 μl samples (in duplicates) were mixed with 100μl Infinity glucose reagent (Thermo Scientific, Cat. N. TR15421) in a 96-well plate ...
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bioRxiv - Genomics 2020Quote: ... Enzyme mix 2 was added and gently mixed using a disposable Pasteur pipette (ThermoFisher, Cat. N: PP89SA). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: Mitochondria were identified by staining the pollen tubes with NAO (10-N nonyl-acridine orange; Molecular Probes) (5 µM).
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bioRxiv - Immunology 2021Quote: ... Gene expression assays were performed using Power SYBR® Green Master Mix (Cat. N. 4368577, ThermoFisher®) with primers described below ...
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bioRxiv - Cell Biology 2022Quote: ... Qiagen) in Optimem reduced serum media and lipofectamine (1μl/100 μl Optimem, RNAiMAX, Invitrogen, Cat. N. 56531) was added to the HUVEC and incubated overnight ...
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bioRxiv - Zoology 2023Quote: ... and 2-(N-morpholino)ethanesulfonic acid (MES) running buffer solution (Bolt™, Thermo Fisher Scientific, Waltham, Massachusetts). A standard ladder (Mark12™ ...
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bioRxiv - Molecular Biology 2022Quote: ... ROS content (n=60; minimum of 20 per group) with CellROX® Green Reagent (CRG) (Thermo Fisher) and MMP (n=60 ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids encoding for c17orf80-BirA* N- or C-terminal fusions were generated using Gateway cloning technology (Invitrogen) using the c17orf80 sequence (NM_001100621 ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: SK-N-BE(2) and NB16 cells were seeded into 8-champer slides (Thermo Fisher Scientific, 154534) with a density of 6×103 cells/well overnight and treated with indicated treatment for 72h ...
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bioRxiv - Cell Biology 2024Quote: N-glycan samples were loaded on a porous graphitic carbon column (100 x 0.32 mm, Thermo Scientific) using 10 mM ammonium bicarbonate buffer as the aqueous solvent (solvent A ...
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bioRxiv - Developmental Biology 2024Quote: ... inserts were then subcloned into pCS2+ N HA tagged vectors that had been converted into Gateway (Invitrogen) destination vectors ...
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bioRxiv - Biochemistry 2024Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Cancer Biology 2024Quote: ... N-glycosylation modification of proteins was assessed using the PNGase F Glycan Cleavage Kit (A39245, Gibco, USA). In brief ...
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bioRxiv - Microbiology 2023Quote: ... N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64) (Thermo Fisher Scientific, Waltham, MA, USA) as previously described (9) ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Microbiology 2023Quote: PC-intact or PC-deleted RhCMV were conjugated with DMSO-dissolved AF647–N-hydroxy succinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Then recombination step was done (Gateway® LR Clonase® II Enzyme mix, INVITROGEN, cat n°11791020) in the final mammalian cellular expression plasmid pEF-Dest51 (InvitrogenTM) ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally biotinylated 67B89 target protein was immobilized on Streptavidin Dynabeads M-270 (Invitrogen, Carlsbad, CA, USA). The target-coated beads were blocked with BSA and incubated with a suspension of 3.4·1012 phages in the presence of a 10-fold molar excess of the off-target (variant 6789) ...
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bioRxiv - Genomics 2023Quote: ... The homogenate was layered above 8mL of sucrose solution in ultracentrifuge tubes (Thermo Scientific, Cat N# 03699) (1mL per tube ...
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bioRxiv - Biochemistry 2022Quote: ... Target protein was coated overnight (O/N) at 4 °C on MaxiSorp™ 96-well plates (Nunc). ELISA-blocking buffer (PBS containing 0.5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: A cDNA encoding p38α was subcloned into pcDNA6 with an N-terminal myc tag (Invitrogen, Carlsbad, CA), and a kinase-dead (D168A ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples (n = 3 technical replicates, each condition) were sorted with an Attune NxT flow cytometer (Thermo Fisher) and the DNA content was analyzed for the percent of cells in G1 ...
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bioRxiv - Neuroscience 2023Quote: ... was added to 10 ml of ice-cold N-PER Neuronal Protein Extraction Reagent (# 87792, Thermo Fisher) by vigorous vortexing until the tablet was completely dissolved ...