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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for N acetylglucosamine 1 phosphodiester alpha N acetylglucosaminidase NAGPA Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Some N-terminal deletion mutations were cloned by PCR using Phusion Polymerase (Thermo Fisher Scientific) followed by ligation using T4 DNA ligase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... The fixed tissue was blocked using 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate; ThermoFisher) diluted in NHS-acetate buffer (0.1M NaP+0.1% Tween PH8 in DEPC H2O ...
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bioRxiv - Neuroscience 2024Quote: Frozen tissues were homogenized in ice-cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) containing protease inhibitor and phosphatase inhibitor cocktail (Cat# PPC1010 ...
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bioRxiv - Cell Biology 2023Quote: ... Enriched N-terminal peptides were then desalted with a C18 spin column (Thermo Fisher Scientific) and the eluate fraction was freeze-dried ...
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bioRxiv - Neuroscience 2024Quote: ... and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N, Gibco, A14700)-coated plates ...
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bioRxiv - Biochemistry 2022Quote: ... each with an N-terminal (His)6 tag from plasmids generated by GeneArt (ThermoFisher Scientific). For each purification ...
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bioRxiv - Cell Biology 2023Quote: ... and 50 units/ml penicillin and 50 μg/ml streptomycin (Gibco; cat n° 5070-063). Cells were cultured at 37 °C in a 5% CO2 gas-equilibrated humidified incubator and passaged every 3-4 days.
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bioRxiv - Cell Biology 2023Quote: ... were cultured in GlutaMAX containing Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, cat n° 31966-047) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-AS cells were cultured in Iscove′s Modified Dulbecco′s medium (IMDM; Thermofisher) with 20% FBS (Gibco) ...
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bioRxiv - Plant Biology 2024Quote: ... rafflesiana n = 3) were determined colorimetrically using the Bradford protein assay kit (Thermo Scientific, USA) with bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection into cultured SK-N-AS cells was performed using Lipofectamine 2000 (Thermo Fisher Scientific) following standard protocols ...
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bioRxiv - Microbiology 2024Quote: ... N sgRNA was detected using TaqMan™ Universal PCR Master Mix (Applied Biosystems – cat # 4304437) and each reaction contain ...
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bioRxiv - Neuroscience 2024Quote: Frozen brain samples were lysed with Neuronal Protein Extraction Reagent (N-Per) (Thermo Scientific # 87792) and Halt Protease inhibitor (Thermo Scientific # 1861278 ...
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Decoding protein glycosylation by an integrative mass spectrometry-based de novo sequencing strategybioRxiv - Biochemistry 2024Quote: N-glycopeptide identification was performed with Biopharma Finder (version 5.2, Thermo Fisher Scientific, Waltham, MA) to search against the de novo sequencing output sequences with corrected Asn-Asp conversion ...
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bioRxiv - Cell Biology 2024Quote: ... N/TERTs were cultured in Keratinocyte Serum-Free Media (K-SFM) (37010022, Gibco, Waltham, MA). For daily maintenance and subculturing of N/TERTs ...
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bioRxiv - Cell Biology 2024Quote: ... coated with 2ml supplemented mTeSRTM 1and 10ng Vitronectin (rhVTN-N, A14700, Life Technologies, Paisley, UK) and 10uM Rock Inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... mass spectrometer interfaced with a Nanospray Flex™ ionization source (Thermo Scientific, P/N ES072) coupled to an Ultimate 3000 ultra-high-pressure chromatography system with 0.1% formic acid in water as mobile phase A and 0.1% formic acid in acetonitrile as mobile phase B ...
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bioRxiv - Cancer Biology 2024Quote: ... and then hybridized to a Clariom S Mouse GeneChip array (Applied Biosystems P/N 902931). Probe signals were normalized and summarized to log2-expression values by using a robust microarray analysis (69 ...
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bioRxiv - Developmental Biology 2023Quote: ... Antibodies used for immunofluorescence-labelling: FITC-conjugated mouse anti-α-tubulin (1:200, Alexa Fluor 488, Invitrogen). DNA was labelled using 10 min incubation in Hoechst 33342 (10 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... and incubated with an anti-Candida antibody coupled to fluorescein isothiocyanate (FITC) (1:500 dilution; ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cell suspensions were mixed with antibodies (1 μl of FITC-CD45 (11-0541-82, Thermo Fisher Scientific), 1.25 μl of PE-F4/80 (12-4801-82 ...
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bioRxiv - Cell Biology 2021Quote: Bovine photoreceptor outer segments (POS) were labeled with FITC using the Pierce FITC Antibody Labeling Kit (Thermo Fisher Scientific). Adherent RPE cells were treated with ~ 5 POS per cell and 1 x 106 POS were applied per RPE organoid ...
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bioRxiv - Neuroscience 2022Quote: ... A similar protocol was established to measure the control anti-FITC antibody utilizing FITC-conjugated BSA (A23015, Molecular Probes) to capture the antibody (Strobel et al. ...
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bioRxiv - Cell Biology 2020Quote: ... labelled with the fluorescent lipid dye BODIPY® FL Maleimide [BODIPY® FL N-(2-Aminoethyl) Maleimide] (Thermo Fisher Scientific; 1:200) and incubated at room temperature in the dark 10 minutes prior to analysis ...
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bioRxiv - Immunology 2021Quote: ... to target the N1 amplicon of the N gene along with TaqPath 1-Step RT-qPCR Master Mix (Applied Biosystems Waltham, MA). For the subgenomic assay ...
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bioRxiv - Microbiology 2021Quote: ... sterols dissolved in chloroform were dried under nitrogen gas (N-EVAP, Organomation) followed by high vacuum for at least 1 hour (Savant SpeedVac, Thermo Fisher Scientific). They were then hydrated with 2.5 mM MβCD prepared in serum-free medium ...
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bioRxiv - Molecular Biology 2020Quote: ... linker oligos with a 5’-terminal amine modification were obtained from IDT (Coralville, Iowa) and activated using sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) reagent (Thermo Fisher, #A39268) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... 30 and 60 min after induction of neutralization of Col 1 and enzymatic gelation (n=2-5 samples/group) of freshly prepared materials with a Multiskan™ GO Microplate Spectrometer (Thermo Scientific). The following solutions were analyzed ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:1000 in PBS for 15 m at RT and mounted on glass microscope slides (Fisher Scientific, cat n. 1157-2203) using VectaShield Vibrant mounting reagent (2B Scientific).
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bioRxiv - Cell Biology 2021Quote: ... co-cultures were fixed in 4% PFA for 30 min and immunostained for the anti-N (protocol described above) and with HCS Cell Mask TM Blue Stain (Invitrogen, 1:300). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Cancer Biology 2023Quote: ... The Flag plasmids were transiently transfected in to N/Tert-1 cells using Lipofectamine™ RNAiMAX transfection (Invitrogen, catalog no. 13778-100) protocol and transfected in to U2OS cells using the calcium phosphate transfection protocol.
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Developmental Biology 2023Quote: ... Two month old kidneys were harvested and isolated kidney cortex was cross-linked with 2 mM Di (N succinimidyl) glutarate (DSG, Proteo Chem C1104) in PBS for 30 min followed incubation in 1% formaldehyde (Thermo Scientific 28906) for an additional 10 min at room temperature and quenched with 125 mM glycine for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and overlaid with 200 μL of alkylation buffer (40 mM HEPES, 50 mM NaCl, 1 mM EGTA, protease inhibitors) supplemented with 25 mM N-ethylmaleimide (NEM; Fisher Scientific, AA4052606). Cells were incubated for 10 minutes at room temperature and then 20 μL of 10% CHAPS detergent (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... Subsamples of 1000–2000 μg were carefully weighed in tin capsules to determine the total N percentage (N% as mg (100 mg DW)−1) and the 15N abundance using a FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific) coupled to a Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... into the medial septal area (MS) of C57Bl/6NTac mice (n=6). For the colocalization experiments (Fig. 1) we injected yellow-green FluoSpheres (Thermo Fisher Scientific) into the MS of two C57Bl/6NTac mice or AAVrg-syn-FLEX-jGCaMP8m-WPRE (Addgene ...
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bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
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bioRxiv - Immunology 2024Quote: ... Four microliters RNA was used RT- qPCR to detect and quantify N gene of SARS-CoV-2 using the TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) with the following primers and probes for the N-gene ...
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bioRxiv - Plant Biology 2024Quote: ... The free thiols of ∼1 mg of LCNP were blocked using 100 mM N-ethyl maleimide (NEM) (Thermo Fisher, Cat. No. 23030) in darkness for 1 h (Muthuramalingam et al. ...
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bioRxiv - Biophysics 2024Quote: ... Samples were stained in DAPI (solution from the RNAscope kit) for 5 minutes or O/N in 1:1000 Spy505 (Spirochrome) and then mounted in ProLong™ Gold (Invitrogen, P36930) in between two coverslips (thickness 1.5mm ...
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bioRxiv - Microbiology 2020Quote: ... in MEM-alpha (Gibco) with 20% FBS (Hyclone) ...
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bioRxiv - Bioengineering 2023Quote: ... in alpha-MEM (Gibco). MSCs were cultured to 80% confluence following standard protocols (65) ...
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bioRxiv - Developmental Biology 2024Quote: ... Alpha-Bungarotoxin (Invitrogen B1601) was injected into the embryonic heart of 24-26 hpf embryos ...
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bioRxiv - Developmental Biology 2021Quote: ... alpha-smooth muscle actin (1:500; Thermo Fisher Scientific, MA1-06110), ACE2 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti IL-1 alpha (Thermo Fisher Scientific; Cat.# 11-7118-81).
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bioRxiv - Cancer Biology 2022Quote: ... PE-Cyanine7_TNF alpha (1:100, Thermo Fisher Scientific, #25-7423-82)
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4 °C and alpha-Tubulin (Invitrogen, 11224-1-AP) for 1-2 hours at room temperature ...